New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 34987659 (1.110)6 (0.190)
+CTACAGTTCAGTTCACCCCGTAGGCCTGATAAGTCGCGTCAGCGGCGCATCAGGCATCG
4/166 4.8 14.8% noncoding (272/365 nt) REP25 (repetitive extragenic palindromic) element; contains 8 REP sequences REP25 (repetitive extragenic palindromic) element; contains 8 REP sequences
?NC_000913_3_pae_tpiA = 349972 NA (NA)intergenic (+400/‑40) prpB/prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACA  >  NC_000913_3_pae_tpiA/349730‑350021
                                                                                                                                                  |                                                                                                                                                  
cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACaa                                                                                                                                                  >  1:278916/1‑149 (MQ=32)
             tGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGCATAAGACGCATAGCGTCGGATCCGACAACCGATGCCTGATg                                                                                                                                     >  1:22688/1‑149 (MQ=255)
                   ggaGGTCGCACAAGACGCATAGCCTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCGTACGGGGTGCACTGAACTGTAGGTCGGATAACACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                                                                                               <  2:277896/147‑1 (MQ=11)
                   ggaGGGCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                                                                                               <  1:899093/147‑1 (MQ=37)
                       gTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGGGTCGCATCCGACAACCGATACCTGATGCGACGCTGAc                                                                                                                           >  1:808914/1‑149 (MQ=255)
                           gATAACACGCATTGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCTATGCCTGATTCGCCGCTGACGCGa                                                                                                                       <  1:810922/149‑1 (MQ=255)
                               aGACGCAGGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTa                                                                                                                   <  2:924252/149‑1 (MQ=17)
                                   gCAGAGCGGCGCATCCGCCAACCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACGGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAg                                                                                                               <  1:880251/149‑1 (MQ=11)
                                     attgCGTCGCACCCGACAACCGATGCCTGATGCGCGGCTGACGCGACGTCTCAGGCCTACGGGGTGCACTGAACTGTAGGTTGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGc                                                                                                             <  2:725055/146‑1 (MQ=11)
                                        ccGTCGCATGCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTa                                                                                                          <  1:506481/148‑1 (MQ=17)
                                          gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg                                                                                                        <  1:350776/149‑1 (MQ=32)
                                              cATCGGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGt                                                                                                    <  2:720150/149‑1 (MQ=17)
                                                 ccGACAACCGATGCCTGATGCGCCGTCGACGGGACTTATCAGGCCCAAGAAGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                                                                 <  1:215070/149‑1 (MQ=11)
                                                     cAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                                                                                              <  2:647385/132‑1 (MQ=32)
                                                     cAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                                                                                              >  1:647385/1‑132 (MQ=33)
                                                      agccGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactgaa                                                                                            <  2:239911/147‑1 (MQ=19)
                                                      aaCCTATGCCTGATGCGCCGCTCACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactgaa                                                                                            <  1:299666/149‑1 (MQ=25)
                                                      aaCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTg                                                                                                                                        <  1:387131/105‑1 (MQ=33)
                                                       aCCGATGCCGGATGCGCCGCGGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactgaac                                                                                           <  2:601417/149‑1 (MQ=25)
                                                        ccGATGCCTGATGCGCCGCTGACGCGACTTATCCGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTgaactgaact                                                                                          <  1:12137/149‑1 (MQ=25)
                                                        ccGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGTTgaactgaact                                                                                          <  1:579187/149‑1 (MQ=25)
                                                        ccGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTgaactgaact                                                                                          <  1:427916/149‑1 (MQ=35)
                                                                  aTGCGGCGCGGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGa                                                                                <  1:701852/149‑1 (MQ=25)
                                                                  aTGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGa                                                                                <  1:584721/149‑1 (MQ=35)
                                                                     cgccggTGAGGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAGGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATaa                                                                             <  2:94486/149‑1 (MQ=255)
                                                                                          ggCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                       <  1:176796/149‑1 (MQ=32)
                                                                                             cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGacaa                                                    >  1:929236/1‑149 (MQ=32)
                                                                                             cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGacaa                                                    >  1:723614/1‑149 (MQ=32)
                                                                                                    ttGCACGGAACTGTAGGTCGGACAAGACGCATAGCGTCGCATCCGACAACCGATGACTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc                                             <  1:8494/148‑1 (MQ=255)
                                                                                                    gTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc                                             <  2:723614/149‑1 (MQ=38)
                                                                                                      gCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTTGCATCCGACAACAATCTCGa                                           >  2:25282/1‑149 (MQ=255)
                                                                                                       cattggaCTGTAGGGGCGATAAGACGGATGGCCACGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc                                          <  2:32174/143‑1 (MQ=11)
                                                                                                       cACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc                                          >  1:531970/1‑149 (MQ=39)
                                                                                                        aCTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATGCGACGCTATGCGTCTTATCCGaccta                                                   >  2:445926/1‑136 (MQ=11)
                                                                                                        aCTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGa                                                                          <  1:205063/116‑1 (MQ=33)
                                                                                                         cTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAccc                                        <  2:929236/149‑1 (MQ=255)
                                                                                                               cgTAGGCCGGATGAGACGCATAGCGTCGCATCCGACAACCGATGCCGGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACaaa                                  <  1:247412/148‑1 (MQ=255)
                                                                                                                  agttCGGACAAGACGCCTAGCGTCCCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGa                               <  1:287392/146‑1 (MQ=255)
                                                                                                                    gTCGGATAAGACGCATAGCGTCGCATCCGACAACCTATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactgaa                                                                                            >  2:818279/1‑87 (MQ=25)
                                                                                                                    gTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactgaa                                                                                            <  1:818279/87‑1 (MQ=35)
                                                                                                                     tCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGt                                                                <  2:310614/113‑1 (MQ=33)
                                                                                                                     tCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATaa                            <  1:880833/149‑1 (MQ=255)
                                                                                                                         aTAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACCGAACTGTAGGTCGGATAAGACGCATAGCGTTGCATCCGACAACAATCTCGACCCTACAAATGATAACAAt                        <  1:25282/149‑1 (MQ=255)
                                                                                                                           aaGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                      <  1:632729/149‑1 (MQ=255)
                                                                                                                              aCGCATAGCGTCGCATCCGACAACCGATGCCTGATGTGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGAACGCTACTGGCATGTCCGCCAACAATCCCTACCCTACAAATGGTAACAATGACGa                   >  1:685219/1‑149 (MQ=14)
                                                                                                                              aCGCAACGCGCCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGa                   <  2:881291/149‑1 (MQ=255)
                                                                                                                                gctatgCGTCTTATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAgg                 >  2:678738/6‑149 (MQ=255)
                                                                                                                                  aTAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactgaa                                                                                            <  1:850157/73‑1 (MQ=34)
                                                                                                                                  aTAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactgaa                                                                                            >  2:850157/1‑73 (MQ=35)
                                                                                                                                    ggCGTCGCATCCGACAACCGATGCCTGATGCGCCACTGACGCGACTTATCAGGCCTACGGGGTCAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGacaa             <  1:833519/148‑1 (MQ=255)
                                                                                                                                    aGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATg                                >  2:526875/1‑130 (MQ=255)
                                                                                                                                    aGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATg                                <  1:526875/130‑1 (MQ=255)
                                                                                                                                        tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGCTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                      <  2:753735/136‑1 (MQ=255)
                                                                                                                                        tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGCTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                      >  1:753735/1‑136 (MQ=255)
                                                                                                                                           cATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGc      <  1:752638/149‑1 (MQ=255)
                                                                                                                                           cATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGc      >  2:746611/1‑149 (MQ=255)
                                                                                                                                             tCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc                                                             <  2:783169/92‑1 (MQ=34)
                                                                                                                                             tCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc                                                             >  1:783169/1‑92 (MQ=34)
                                                                                                                                             tCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGa    <  2:602727/149‑1 (MQ=255)
                                                                                                                                               cGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGaca  <  1:933519/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACA  >  NC_000913_3_pae_tpiA/349730‑350021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.