Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	3,815,859	Δ82 bp		[rph]–[rph]	[rph], [rph]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	3815859	3815940	82	47 [0]	[0] 47	[rph]–[rph]	[rph],[rph]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3815858	0 (0.000)	44 (0.890)	39/264	0.3	100%	pseudogene (24/48 nt)	rph	ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
	?	NC_000913_3_pae_tpiA	3815941 =	0 (0.000)					pseudogene (609/669 nt)	rph	ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

TACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATC  >  NC_000913_3_pae_tpiA/3815794‑3816088                                                                                                                                                    |                                                                                                                                                    ctttaacacctgcttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccataacatttagttagccagcgccgccttctgcCg                                                                                                                                                    >  2:193403‑M2/148‑149 (MQ=255)     aacacctgcttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTccc                                                                                                                                                <  1:298560‑M2/6‑1 (MQ=255)        acctgcttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTg                                                                                                                                             >  2:811357‑M2/141‑149 (MQ=255)         cctgcttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcaatttggccgcgctgccttctgcCGTCCCCGGc                                                                                                                                            >  1:912815‑M2/140‑149 (MQ=255)                gctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaacaaggcgactcatcagtcgccttaaacatcagtttgccagcgccgccttctgcCGTCCCCGGCACttcca                                                                                                                                     >  1:63545‑M2/133‑147 (MQ=255)                cataaccgcaaattcaataaaccggcgctgatatggtttcacgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtccccttaaaactcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCaa                                                                                                                                     <  1:716494‑M2/17‑1 (MQ=255)                 ctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcattttgccagcacctccttctgcCGTCCCCTGCACTTCAat                                                                                                                                    >  1:353504‑M2/132‑149 (MQ=255)                 ctaagcgcaaactcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAat                                                                                                                                    <  1:189382‑M2/18‑1 (MQ=255)                 ccaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcaccgccttctgcCGTCCCCTGCACTTCAat                                                                                                                                    >  1:353481‑M2/132‑149 (MQ=255)                    agcgcaaattcaataaactggcgcagatctgaagagagtccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAatgat                                                                                                                                 >  2:441425‑M2/129‑149 (MQ=255)                      cgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATgc                                                                                                                               >  1:606973‑M2/127‑149 (MQ=255)                       gcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGT‑CCCTGCACTTCAATGATgcgc                                                                                                                             >  1:112852‑M2/126‑149 (MQ=255)                       gcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGCCCCCTGCACTTCCATGAtgca                                                                                                                              >  1:484650‑M2/126‑148 (MQ=255)                        caaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATgcgc                                                                                                                             <  2:730614‑M2/25‑1 (MQ=255)                          aattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaacaaaggcgactcatcagtcgccttcaaaatctgcttgccaccgccgccttctgcCGTCCCCCGCCCTTCCATCATGCcccc                                                                                                                           >  2:725053‑M2/123‑149 (MQ=255)                                    actggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagaaaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtc                                                                                                                 <  2:698319‑M2/37‑1 (MQ=255)                                      tggcgctgatatggtttcatgccttcgctcctcatcttgcttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                                                                                               >  2:821136‑M2/111‑149 (MQ=255)                                        gcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                                                                                             <  1:40829‑M2/41‑1 (MQ=255)                                             gatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACg                                                                                                        >  1:790050‑M2/104‑149 (MQ=255)                                              atatggtttcatgccttcgctcctcatcttacttttctccatacaaacaaaaggcgactcatcagtcgccttaaaaatcagtttcccagcgccgccttctacCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGCTAATCACtactt                                                                                                       >  2:614915‑M2/103‑147 (MQ=255)                                              atatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                                                                                                       >  1:395257‑M2/103‑149 (MQ=255)                                                atggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                                                                                                     >  1:482705‑M2/101‑149 (MQ=255)                                                      tcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                                                                                               <  2:837337‑M2/55‑1 (MQ=255)                                                      tcatgccttcgcccctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                                                                                               <  2:173577‑M2/55‑1 (MQ=255)                                                           ccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtctc                                                                                          >  1:315458‑M2/90‑149 (MQ=255)                                                                  tcctcatcttacttttctacagacaaaaaaaaggcggctcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCACTGATGCGCCCGTCTTCGGTAATCACGACGTTCATATCGTGCTCTGCGGCa                                                                                   >  2:401340‑M2/83‑149 (MQ=255)                                                                    ctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTTGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga                                                                                 >  1:175601‑M2/81‑149 (MQ=255)                                                                    ctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccaacgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTACTCACTACGTTCATGTCGGTCTCTGcggccgc                                                                                 >  2:405290‑M2/81‑146 (MQ=255)                                                                    cacaccttacttccctacagacaaaaaaaaggcgactcctcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga                                                                                 <  1:24949‑M2/69‑1 (MQ=255)                                                                       atcttgcgtttcgacagacaaaaaaaaggcgtctcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCTCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc                                                                              <  2:518418‑M2/72‑1 (MQ=255)                                                                        tcttacctttccacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt                                                                             <  1:932653‑M2/73‑1 (MQ=255)                                                                         cttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGCTGCGCCCGTCTTCGGTCCTCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCtt                                                                            >  1:209188‑M2/76‑149 (MQ=255)                                                                                       gacaaaaaaaaggcgactcatcagtcgccttaaacatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGa                                                              <  1:114646‑M2/88‑1 (MQ=255)                                                                                             aaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCaa                                                        >  2:290236‑M2/56‑149 (MQ=255)                                                                                                   gcgactgatgagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCt                                                  >  1:765694‑M2/50‑149 (MQ=255)                                                                                                          atcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGt                                           <  2:658164‑M2/107‑1 (MQ=255)                                                                                                               tcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTAATGTCGGTGTTACCTGCAGAGCCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTcaca                                      >  1:648905‑M2/38‑149 (MQ=255)                                                                                                                cgccttaaaaatctgttgtccagctccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTTCGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACaa                                     <  1:193403‑M2/113‑1 (MQ=255)                                                                                                                       aaaatctgtgtgccagcgccgtcttctgcCGTCCCCTGCACTTCAATGATGCGCCCTTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGAc                              <  2:65839‑M2/120‑1 (MQ=255)                                                                                                                             agtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAAc                        >  1:354710‑M2/24‑149 (MQ=255)                                                                                                                               tttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGt                                                                               >  2:426532‑M2/22‑92 (MQ=255)                                                                                                                               tttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGt                                                                               <  1:426532‑M2/71‑1 (MQ=255)                                                                                                                               gttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTg                      <  1:531215‑M2/128‑1 (MQ=255)                                                                                                                                         ccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCAt            <  2:606973‑M2/138‑1 (MQ=255)                                                                                                                                              ttctgcCGTCCCCTGCATTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCtt       <  2:536668‑M2/143‑1 (MQ=255)                                                                                                                                              ttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCtt       >  1:605002‑M2/7‑149 (MQ=255)                                                                                                                                                   cCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATc  <  2:354710‑M2/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATC  >  NC_000913_3_pae_tpiA/3815794‑3816088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑