Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,594,214 C→A R549L (CGT→CTT)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,594,2140CA96.0% 443.4 / ‑0.3 126R549L (CGT→CTT) tmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (61/60);  total (62/64)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

AAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCAT  >  NC_000913_3_pae_tpiA/2594067‑2594360
                                                                                                                                                   |                                                                                                                                                  
aaGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGt                                                                                                                                                  <  2:216785/150‑1 (MQ=255)
 aGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTa                                                                                                                                                 >  2:1412143/1‑150 (MQ=255)
   cAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTaaa                                                                                                                                               <  4:887761/150‑1 (MQ=255)
    aaCCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAAc                                                                                                                                              >  4:113066/1‑150 (MQ=255)
       cTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGt                                                                                                                                           <  4:561828/150‑1 (MQ=255)
        tAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTa                                                                                                                                          >  2:67313/1‑150 (MQ=255)
         aaCGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTaa                                                                                                                                         <  2:754909/150‑1 (MQ=255)
            gacgTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGc                                                                                                                                      <  2:1438484/150‑1 (MQ=255)
              cgTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTc                                                                                                                                    >  2:1380767/1‑150 (MQ=255)
               gTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCa                                                                                                                                   <  2:297133/150‑1 (MQ=255)
                 tAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACg                                                                                                                                 >  3:249393/1‑150 (MQ=255)
                  aaTAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGt                                                                                                                                >  4:281264/1‑150 (MQ=255)
                    tAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTc                                                                                                                              <  2:687567/150‑1 (MQ=255)
                     aGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTca                                                                                                                             >  1:954036/1‑150 (MQ=255)
                             aTGAGCGACAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTg                                                                                                                     <  2:1288133/150‑1 (MQ=255)
                              tGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGt                                                                                                                    >  4:131415/1‑150 (MQ=255)
                              tGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGt                                                                                                                    <  3:281264/150‑1 (MQ=255)
                                aGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGttt                                                                                                                  >  3:825701/1‑150 (MQ=255)
                                 gCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTa                                                                                                                 <  2:621464/150‑1 (MQ=255)
                                  cGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCATGTTca                                                                                                                             >  1:1137674/1‑137 (MQ=255)
                                  cGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCATGTTca                                                                                                                             <  2:1137674/137‑1 (MQ=255)
                                  cGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTAc                                                                                                                <  1:1380767/150‑1 (MQ=255)
                                   gAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTAcc                                                                                                               <  3:575230/150‑1 (MQ=255)
                                   gAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTAcc                                                                                                               <  3:117347/150‑1 (MQ=255)
                                    aaaGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCCTCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTAccc                                                                                                              <  1:1121363/150‑1 (MQ=255)
                                    aaaGCAAAACCGGCCAGTTCAAGCCAGTCGTCGCCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACAGCCCGAGAGCTCGCGCATCGCGCAGTAAACGGTAACGCTCACGTTCAGCCAGCTGTTTAccc                                                                                                              >  4:276462/1‑150 (MQ=255)
                                      aGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGc                                                                                                            <  2:118898/150‑1 (MQ=255)
                                      aGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGc                                                                                                            <  2:1278332/150‑1 (MQ=255)
                                        caacgccGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGACTCAACAAAAAGGCTTTCGCCATTGCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCAt                                                                                                          <  1:1453227/145‑1 (MQ=255)
                                        cAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTca                                                                                                                             >  4:876302/1‑131 (MQ=255)
                                        cAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTca                                                                                                                             <  3:876302/131‑1 (MQ=255)
                                        cAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCATGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCAt                                                                                                          <  1:850715/150‑1 (MQ=255)
                                         aaaaCCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATc                                                                                                         >  2:1107751/1‑150 (MQ=255)
                                          aaaCCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGCGCTTGCGCATCGCTAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCa                                                                                                        >  1:594344/1‑150 (MQ=255)
                                           aaCCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCAc                                                                                                       >  1:611256/1‑150 (MQ=255)
                                              cGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTaa                                                                                                                                         <  3:496568/113‑1 (MQ=255)
                                              cGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTaa                                                                                                                                         >  4:496568/1‑113 (MQ=255)
                                              cGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCa                                                                                                    >  3:545625/1‑150 (MQ=255)
                                                gCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATc                                                                                                  >  2:332243/1‑150 (MQ=255)
                                                  cAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCgg                                                                                                <  2:604450/150‑1 (MQ=255)
                                                       cAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACa                                                                                           >  1:269747/1‑150 (MQ=255)
                                                       cAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACa                                                                                           >  4:849566/1‑150 (MQ=255)
                                                       cAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACa                                                                                           >  1:158407/1‑150 (MQ=255)
                                                           ccAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAgcgc                                                                                       >  3:563454/1‑150 (MQ=255)
                                                                  tcgtcAGAAAGGATGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGcc                                                                                <  2:1276968/150‑1 (MQ=255)
                                                                      cAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGt                                                                                               >  1:623571/1‑131 (MQ=255)
                                                                       aGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGtata                                                                           >  4:281756/1‑150 (MQ=255)
                                                                       aGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGtata                                                                           >  1:1010019/1‑150 (MQ=255)
                                                                        gAAGGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGACGTATAg                                                                          >  3:824832/1‑150 (MQ=255)
                                                                        gAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAg                                                                          <  3:685803/150‑1 (MQ=255)
                                                                                ggCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgct                                                                  <  1:955160/150‑1 (MQ=255)
                                                                                 gCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgctg                                                                 <  3:131415/150‑1 (MQ=255)
                                                                                   aTCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTAcc                                                                                                               <  1:136439/102‑1 (MQ=255)
                                                                                   aTCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgctgct                                                               <  2:1010019/150‑1 (MQ=255)
                                                                                   aTCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgctgct                                                               >  4:322897/1‑150 (MQ=255)
                                                                                   aTCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGATAACAGCGCCATCGCCGTATAGCAACCgctgct                                                               >  2:830422/1‑150 (MQ=255)
                                                                                   aTCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCCCAGCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgctgct                                                               <  1:92097/150‑1 (MQ=255)
                                                                                    tCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGctgctg                                                              <  4:563454/150‑1 (MQ=255)
                                                                                    tCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGctgctg                                                              <  1:891591/150‑1 (MQ=255)
                                                                                    tCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGctgctg                                                              <  3:113066/150‑1 (MQ=255)
                                                                                       tttAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCt                                                           >  2:209999/1‑150 (MQ=255)
                                                                                          aGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTcc                                                        >  4:714658/1‑150 (MQ=255)
                                                                                            tGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCg                                                      <  1:1412143/150‑1 (MQ=255)
                                                                                              gATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCgat                                                    <  2:901307/150‑1 (MQ=255)
                                                                                               aTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTcagcca                                                                                                                         >  1:417625/1‑80 (MQ=255)
                                                                                               aTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTcagcca                                                                                                                         <  2:417625/80‑1 (MQ=255)
                                                                                                 cAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGtat                                                                            >  2:844395/1‑123 (MQ=255)
                                                                                                 cAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGtat                                                                            <  1:844395/123‑1 (MQ=255)
                                                                                                   aCAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGc                                                                                                            <  1:34440/89‑1 (MQ=255)
                                                                                                   aCAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGc                                                                                                            >  2:34440/1‑89 (MQ=255)
                                                                                                   aCAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTa                                               <  4:825701/150‑1 (MQ=255)
                                                                                                    cAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTAc                                              <  4:656383/150‑1 (MQ=255)
                                                                                                    cAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTAc                                              <  1:1196614/150‑1 (MQ=255)
                                                                                                     aGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTAcc                                             >  3:704714/1‑150 (MQ=255)
                                                                                                      ggAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTAccc                                            >  4:621511/1‑150 (MQ=255)
                                                                                                       gAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgctgc                                                                >  1:1005483/1‑129 (MQ=255)
                                                                                                       gAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGATAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCa                                           >  1:312906/1‑150 (MQ=255)
                                                                                                        aaGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCAt                                          >  1:678247/1‑150 (MQ=255)
                                                                                                               tCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCAcc                                   <  3:322897/150‑1 (MQ=255)
                                                                                                                cGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCa                                  <  2:1087416/150‑1 (MQ=255)
                                                                                                                 gCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAg                                 >  3:648067/1‑150 (MQ=255)
                                                                                                                 gCCATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAg                                 >  2:572637/1‑150 (MQ=255)
                                                                                                                  ccATTCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCCGc                                >  1:1001540/1‑150 (MQ=255)
                                                                                                                    cgtttaCTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGcac                              >  1:1341478/6‑150 (MQ=255)
                                                                                                                      tCCACTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACaa                            >  1:1221670/1‑150 (MQ=255)
                                                                                                                         aCTGCGCGAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAc                         >  3:729954/1‑150 (MQ=255)
                                                                                                                           ttcgcgAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCg                       >  1:649163/3‑150 (MQ=255)
                                                                                                                              gcgAGAGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAg                    <  1:492034/150‑1 (MQ=255)
                                                                                                                                gagaGCTTGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCg                  >  3:565699/1‑150 (MQ=255)
                                                                                                                                    gCTTGCGCACCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCGATCCGCACCAGCAAAAAACCGCAGCGTTGc              <  2:752164/150‑1 (MQ=255)
                                                                                                                                      ttGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGc                                                                         >  2:136912/1‑89 (MQ=255)
                                                                                                                                      ttGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGc                                                                         <  1:136912/89‑1 (MQ=255)
                                                                                                                                      ttGCGCATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCa            >  3:785669/1‑150 (MQ=255)
                                                                                                                                           cATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAAc       <  4:561782/150‑1 (MQ=255)
                                                                                                                                           cATCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAAc       >  3:108565/1‑150 (MQ=255)
                                                                                                                                             tCGCGAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCACCCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGc     <  2:611256/150‑1 (MQ=255)
                                                                                                                                              cgcgAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCAc                                                                                                       <  2:88419/51‑1 (MQ=255)
                                                                                                                                                cgAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGc                                >  1:1015623/1‑120 (MQ=255)
                                                                                                                                                cgAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCAt  >  2:1452128/1‑150 (MQ=255)
                                                                                                                                                cgAAGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACGCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCAt  >  4:167766/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCAT  >  NC_000913_3_pae_tpiA/2594067‑2594360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: