Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 257908–258675 258683 9–776 106 [0] [0] 106 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 2579070 (0.000)101 (0.910) 64/264 0.9 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_pae_tpiA 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 106 total aligned reads displayed.

TTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_pae_tpiA/257763‑258055
                                                                                                                                                |                                                                                                                                                    
ttGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                                                                  >  1:888727‑M1/1‑145 (MQ=255)
   tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAggtgcaa                                                                                                                                               >  2:2052313‑M1/1‑142 (MQ=255)
     ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATGGCAATGACGTTACCGAGTGGACACCCGAAGAGCAAATTGATCAAAAAATTTACCGCACTAGACCCGTATATTCGAGAAGGTAagtgcaagg                                                                                                                                             >  1:142421‑M1/1‑140 (MQ=255)
     ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCGGATTGATCAAAAAATTTACCGCACTAGGCCCGAAGATTCGTGAAGATAagtgcaaag                                                                                                                                             >  1:482248‑M1/1‑140 (MQ=255)
     ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaag                                                                                                                                             >  2:791829‑M1/1‑140 (MQ=255)
         aaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATATCAATGACGTTACCGAGTGGACACCCGAAGAGCAGCTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaacgataa                                                                                                                                         >  2:196697‑M1/1‑136 (MQ=255)
         aaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataa                                                                                                                                         >  2:2074347‑M1/1‑136 (MQ=255)
          aaTTTGTTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGCATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                        <  2:1775244‑M1/149‑15 (MQ=255)
          aaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGATATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                        <  2:1164903‑M1/149‑15 (MQ=255)
          aaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                        >  2:644312‑M1/1‑135 (MQ=255)
          aaTTTGATAAAATTTGCCAATTTGGGAAAACAGTTGCATCACAACAGGATATAGTAATGACGTTACCGAGTGGACACCCGAAGAGAAAAAAAAAAAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                        <  2:1164907‑M1/149‑15 (MQ=255)
                 tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                 >  2:687256‑M1/1‑128 (MQ=255)
                 tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATAACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                 >  1:1110276‑M1/1‑128 (MQ=255)
                   aaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGCCGTTACCGATTGGACACCCGAAGAGCAGATTGATCGAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttt                                                                                                                               >  2:1849420‑M1/1‑126 (MQ=255)
                     ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                             <  2:652735‑M1/149‑26 (MQ=255)
                     tgttgcCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGCCACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                             <  2:1849696‑M1/147‑26 (MQ=255)
                      ttggccaATTTGGTAAAACAGTTGAAACACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGAAAGAAAAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                            <  2:819292‑M1/146‑27 (MQ=255)
                         gCCAATTTGGTAAAACAGCTGTATCACAACAAGAGATAGCAATGACGTTACCGAGTGGAAACCCGAAGAGCAGATTGAACAAAAAATTTACCGCACTAGGCCCGTATATTCTTGAAGGTAagtgcaaagataatcgattctttttcgat                                                                                                                         <  2:396506‑M1/149‑30 (MQ=255)
                          ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGTAGGTAagtgcaaagataatcgattctttttcgatt                                                                                                                        <  2:1825050‑M1/149‑31 (MQ=255)
                          ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatt                                                                                                                        >  2:1931737‑M1/1‑119 (MQ=255)
                             aTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGAGGACACCCAAAGAACAGATTGATCAAAAAATTTACCGCACTCGGCCAGTAAATTCGTGAAGGTAagcacaaaaataatcgattctttttcgattatc                                                                                                                     >  2:1967092‑M1/1‑116 (MQ=255)
                               ttGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctg                                                                                                                   <  2:1417846‑M1/149‑36 (MQ=255)
                                tGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  <  1:266614‑M1/149‑37 (MQ=255)
                                gtgtaAAACAGTGGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  <  2:931719‑M1/147‑37 (MQ=255)
                                  gTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgactctttttcgattgtctggct                                                                                                                >  2:629079‑M1/1‑111 (MQ=255)
                                    aaaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgt                                                                                                              >  1:556227‑M1/1‑109 (MQ=255)
                                     aaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                                                                             >  1:1263831‑M1/1‑108 (MQ=255)
                                           ttGCATCACAACAGGAGATAGCAATGACGTTCCCGAGTGGACACCCGAAGAGCAGATTGATCAAAACATTTACCGCACTAGGCCCGTATCTTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggcggtatgcgtc                                                                                                       >  1:1282559‑M1/1‑102 (MQ=255)
                                             gCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaa                                                                                                     >  1:223658‑M1/1‑100 (MQ=255)
                                              cATCACAACAGGGGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTACATTCGTGCAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                    <  1:1124827‑M1/149‑51 (MQ=255)
                                               aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtttggctgtatgagtcaacg                                                                                                   >  2:189515‑M1/1‑98 (MQ=255)
                                                tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGGGCAGATTGATCACAAAATTTACCGCACTCGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgcttcattttcgattatctagctcgatgcgtccacgt                                                                                                  >  2:898435‑M1/1‑97 (MQ=255)
                                                tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  <  2:1132853‑M1/133‑37 (MQ=255)
                                                tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  >  1:1132853‑M1/1‑97 (MQ=255)
                                                tCAAAACAGGAGATGACAATGACGTTACCGAGTGGACACCCGAAGAACAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                                  <  1:1800639‑M1/149‑53 (MQ=255)
                                                  acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtga                                                                                                <  2:261782‑M1/149‑55 (MQ=255)
                                                  acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGCATATGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtga                                                                                                <  1:1811829‑M1/149‑55 (MQ=255)
                                                    aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGGTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcacggtgaaa                                                                                              >  1:1771029‑M1/1‑93 (MQ=255)
                                                    aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaa                                                                                              >  1:365722‑M1/1‑93 (MQ=255)
                                                                     aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcg                                                                             <  1:1362674‑M1/149‑74 (MQ=255)
                                                                        ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                                  <  2:607374‑M1/125‑53 (MQ=255)
                                                                        ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                                  >  1:607374‑M1/1‑73 (MQ=255)
                                                                         tACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaa                                                                         <  1:1931737‑M1/149‑78 (MQ=255)
                                                                         cacCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaa                                                                         <  1:369513‑M1/148‑78 (MQ=255)
                                                                             gAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgcattct                                                                     >  1:1079707‑M1/1‑68 (MQ=255)
                                                                             gAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagagcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtggattct                                                                     >  1:1337769‑M1/1‑68 (MQ=255)
                                                                              aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                    <  1:1075662‑M1/149‑83 (MQ=255)
                                                                               gTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                   >  2:1742963‑M1/1‑66 (MQ=255)
                                                                                tGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggg                                                                  >  1:847735‑M1/1‑65 (MQ=255)
                                                                                 ggACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggg                                                                 <  2:944159‑M1/149‑86 (MQ=255)
                                                                                  gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacctgcaccggaaatgccttaattttggtgc                                                                >  2:1614029‑M1/1‑63 (MQ=255)
                                                                                  gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggc                                                                >  1:960015‑M1/1‑63 (MQ=255)
                                                                                  gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtaaacgtgaaaccggcaccggaagtgcgtgaattctgggga                                                                >  2:246049‑M1/1‑63 (MQ=255)
                                                                                       ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcacagataatcgattctttttcgattgtctggctttatcattcaacgtgaacccggccccggagatgcgtgaattcttggtcgggtg                                                           >  2:514392‑M1/1‑58 (MQ=255)
                                                                                       ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                           >  1:1717837‑M1/1‑58 (MQ=255)
                                                                                        cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggccccggaagtgcgtgaattctggggcgggtgg                                                          >  1:2075143‑M1/1‑57 (MQ=255)
                                                                                         gAAGAGCCGATTAATCAGAAAATTTACCGCACTAGGCCTGTATTTTTGTTAAGGTAagtgcaacgataatcgattctttttcgattgtatggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                         <  2:142421‑M1/149‑94 (MQ=255)
                                                                                           agagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                       <  1:1929091‑M1/149‑96 (MQ=255)
                                                                                           agagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                       <  2:1929751‑M1/149‑96 (MQ=255)
                                                                                              ggagaTGGAACAAAATATGTAACGTACTAGGCCCGTATATTCGGGAAGGTAagtccaaagataatcgaatcttttgcgattgtctggcggtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggag                                                    <  2:886505‑M1/147‑99 (MQ=255)
                                                                                              gCAGATTGATCAAAAAATTAACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaacccggctcctgaagtgcgtgatttctgtggctggtggatggag                                                    <  2:888507‑M1/149‑99 (MQ=255)
                                                                                               cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtcagcgtcgacgtgcaaccggcaccgaaggtgcgtgcaaactggggctggagggtggagc                                                   >  1:436823‑M1/1‑50 (MQ=255)
                                                                                               cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat                                                        >  2:173615‑M1/1‑50 (MQ=255)
                                                                                               cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat                                                        <  1:173615‑M1/144‑95 (MQ=255)
                                                                                                aGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagct                                                  <  2:223658‑M1/149‑101 (MQ=255)
                                                                                                  atggatcAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgggggtatgcgtgcacgtgaaaccggcaccgaaagcgcgtgagttctggggctggtgggtggagatgg                                                >  2:182329‑M1/4‑47 (MQ=255)
                                                                                                  aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                <  1:1742963‑M1/149‑103 (MQ=255)
                                                                                                  aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                >  1:127277‑M1/1‑47 (MQ=255)
                                                                                                  aTTGATCAAAAAATTTACCGCAACAAGCGAGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                <  2:436823‑M1/149‑103 (MQ=255)
                                                                                                  aTTGAACAAAAAATTTACCGCACTCGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                <  1:969183‑M1/149‑103 (MQ=255)
                                                                                                      aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagc                                            >  2:1611664‑M1/1‑43 (MQ=255)
                                                                                                      aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggag                                                    <  1:953419‑M1/141‑99 (MQ=255)
                                                                                                       tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                           >  2:113157‑M1/1‑42 (MQ=255)
                                                                                                       tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggt                                                            <  2:604886‑M1/132‑91 (MQ=255)
                                                                                                       tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggt                                                            >  1:604886‑M1/1‑42 (MQ=255)
                                                                                                         aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaacccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  1:358153‑M1/1‑40 (MQ=255)
                                                                                                         aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgcattgtggtgctggtggatggagctggaagcgca                                         >  2:1119947‑M1/1‑40 (MQ=255)
                                                                                                         aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  2:1775232‑M1/1‑40 (MQ=255)
                                                                                                         aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  2:931151‑M1/1‑40 (MQ=255)
                                                                                                          aaaaaTTTACCGCCCTAGGCCCTTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                        <  2:1111970‑M1/149‑111 (MQ=255)
                                                                                                          aaaaaTGTACCGCACTTGGCCCGTATATTCTGTAAGGTGagtggaaagataaacgattctgtgtcgattgtcttgctgtatgcgtgaacgtgaaaccggccccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                        <  2:1282559‑M1/149‑111 (MQ=255)
                                                                                                           aaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccg                                                                                           <  2:845417‑M1/97‑60 (MQ=255)
                                                                                                           aaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccg                                                                                           >  1:845417‑M1/1‑38 (MQ=255)
                                                                                                                    cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagcagaaagcgcaggaatcccgtt                              >  2:1529592‑M1/1‑29 (MQ=255)
                                                                                                                    cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttgcgattgtctggctgtatgcgtcaacgtggaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtt                              <  1:1914131‑M1/149‑121 (MQ=255)
                                                                                                                      cACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttgcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggattgacctggcagcgcaggaatcccgtttt                            >  2:2021633‑M1/1‑27 (MQ=255)
                                                                                                                        cTAGGCCCGGATAGTCGGTAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                          <  2:365722‑M1/149‑125 (MQ=255)
                                                                                                                        cTAGGCCCGAATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgggaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                          <  1:687256‑M1/149‑125 (MQ=255)
                                                                                                                            gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgacttctggggctggtggatgaagctggaagcgcaggaatcccgttttacctac                      >  2:1768797‑M1/1‑21 (MQ=255)
                                                                                                                            gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                        >  1:1388433‑M1/1‑21 (MQ=255)
                                                                                                                            gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                        <  2:1388433‑M1/131‑111 (MQ=255)
                                                                                                                               cGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttttttttttttttctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaagtctggggctggtggatggagctggaagcgcaggagtcccgttttacctacagt                   >  2:1599815‑M1/1‑18 (MQ=255)
                                                                                                                               cGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                >  2:1271690‑M1/1‑18 (MQ=255)
                                                                                                                               cGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                <  1:1271690‑M1/120‑103 (MQ=255)
                                                                                                                               cGTATATTCGTGAAGGTAagtacaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagt                   <  1:1482007‑M1/149‑132 (MQ=255)
                                                                                                                                    atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca              <  1:107740‑M1/149‑137 (MQ=255)
                                                                                                                                         tGAAGGTAagtgcaaagatgatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg         >  2:1761715‑M1/1‑8 (MQ=255)
                                                                                                                                         tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg         <  1:1192403‑M1/149‑142 (MQ=255)
                                                                                                                                                aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  2:556227‑M1/149‑149 (MQ=255)
                                                                                                                                                aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  1:149072‑M1/149‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_pae_tpiA/257763‑258055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: