Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,817,1700CA100.0% 358.6 / NA 110E112* (GAA→TAA) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (45/65);  total (45/65)

Only 100 of 110 total aligned reads displayed.

CGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGG  >  NC_000913_3_pae_tpiA/2817027‑2817313
                                                                                                                                                |                                                                                                                                                
cGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTcc                                                                                                                                             <  1:580156/149‑1 (MQ=255)
cGGTTGCACGCCCACAGCCGGCTCGCCCAACAAAAATCTTGTCGGCGCGGGTTTATGGTGTTGGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGTCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTcc                                                                                                                                             <  1:921742/149‑1 (MQ=255)
 ggTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTccc                                                                                                                                            <  1:677387/149‑1 (MQ=255)
  gTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCACTGCCTCAGCGATGGCGCTTTAACTcccc                                                                                                                                           >  1:645456/1‑149 (MQ=255)
      cACCCCCATACGGTGCGCGCACAACAAAAATGTGTCTGGCGCGGATTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                       <  2:1656720/149‑1 (MQ=255)
       aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGGGCGGGTTTATGGTGTTTGACCTGATCAGCAGCGACGACGGCGGCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCTCTTTAACTCGCCGTTcc                                                                                                                                      >  2:1173095/1‑149 (MQ=255)
          cccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTAc                                                                                                                                   <  1:62180/149‑1 (MQ=255)
          cccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTAc                                                                                                                                   <  2:704308/149‑1 (MQ=255)
           ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACa                                                                                                                                  <  1:1663105/149‑1 (MQ=255)
            cATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAg                                                                                                                                 >  2:1340941/1‑149 (MQ=255)
                   tGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTGATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTgg                                                                                                                          <  1:1809777/149‑1 (MQ=255)
                     cgcgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTACGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGc                                                                                                                        >  1:1258654/1‑149 (MQ=255)
                        gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac                                                                                                                    >  1:1561437/1‑149 (MQ=255)
                          acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacga                                                                                                                  >  2:352730/1‑149 (MQ=255)
                            aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc                                                                                                                >  1:1155742/1‑149 (MQ=255)
                            aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc                                                                                                                >  1:339426/1‑149 (MQ=255)
                             acaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCGTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCa                                                                                                               >  1:941884/1‑149 (MQ=255)
                                aaaaTGTGTCTGGCGCGGGTTTAAGGTGTTGGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGc                                                                                                            <  2:1776455/149‑1 (MQ=255)
                                      gtgtCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACt                                                                                                       <  1:162853/149‑1 (MQ=255)
                                         tCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGTGCGGAAATATCCCGTGCCGACAGCCATTGGGCGACGACCATGCCAACtttt                                                                                                    >  1:162211/1‑149 (MQ=255)
                                         tCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtttt                                                                                                    <  2:645456/149‑1 (MQ=255)
                                           tGGCGGGGGGTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                  <  2:1859768/149‑1 (MQ=255)
                                           tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                  <  2:1507598/149‑1 (MQ=255)
                                           cggCGCGGGGTTATGGTGTGTGACGTGATCGGCAGCGAAGACGGCGTCAAAATAAGGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                  <  2:529766/148‑1 (MQ=255)
                                            ggCGCGGGTTTATGGTGTTTGACGTGATCGGCAACGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTGCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcac                                                                                                 <  2:682062/149‑1 (MQ=255)
                                              cgctGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATACTGGCGTAATCCCATGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACCCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcacca                                                                                               <  2:1970230/149‑1 (MQ=255)
                                               gcgAGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCACGTGCGCCAGCAATGCCTCAGCGATGGCCCTTTAACTCCCCGTTCCTACAGCCATTGGGCGCACCACCTGCCAACTTTTcacccc                                                                                              >  2:264405/1‑147 (MQ=255)
                                                 gggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAATTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACAt                                                                                            <  1:1453552/149‑1 (MQ=255)
                                                 gggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACAt                                                                                            <  1:1074608/149‑1 (MQ=255)
                                                  ggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATc                                                                                           <  2:1630721/149‑1 (MQ=255)
                                                       aTGGGGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACGTcaacaa                                                                                      <  2:406307/149‑1 (MQ=255)
                                                           tgtTTGACGTGATCGGCAGCGACGAGGGCGTCAAAATAATGGCGTAATCGCAGGTGCGCCAGCAATGCCTAAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAGCATGCCAACTTTTCAACACATTAACCAGAgg                                                                                  >  2:1014783/1‑149 (MQ=255)
                                                            gtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGa                                                                                 >  2:1587090/1‑149 (MQ=255)
                                                             tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAggga                                                                                >  1:473494/1‑147 (MQ=255)
                                                              ttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAg                                                                               <  2:171491/149‑1 (MQ=255)
                                                              gtggcGTGACCGGCAGCGACGAGGGGGTGAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAg                                                                               <  2:342960/145‑1 (MQ=255)
                                                                 aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGGCTCAGCGATGGCGCTTTAACTCCCCGTTACTACAGCCATTGGGCGACGACCATGCCAACTTTTCACTACATCAACAAGAGGAAGCgg                                                                            >  2:1818204/1‑149 (MQ=255)
                                                                 aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCgg                                                                            <  2:1257697/149‑1 (MQ=255)
                                                                   gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                                                                          <  2:422986/149‑1 (MQ=255)
                                                                     gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCg                                                                        >  2:1348935/1‑149 (MQ=255)
                                                                      aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGa                                                                       <  1:453668/149‑1 (MQ=255)
                                                                      aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGa                                                                       <  2:1546788/149‑1 (MQ=255)
                                                                          gtaGCGACGACGGCGACAAAATAAGGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCt                                                                   <  2:1342655/147‑1 (MQ=255)
                                                                               gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCca                                                              <  2:736775/149‑1 (MQ=255)
                                                                               gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCca                                                              <  1:2006674/149‑1 (MQ=255)
                                                                                acgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcag                                                             <  2:844536/149‑1 (MQ=255)
                                                                                acgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcag                                                             <  1:1348935/149‑1 (MQ=255)
                                                                                acgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcag                                                             <  2:339426/149‑1 (MQ=255)
                                                                                  gacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                           >  2:744854/1‑149 (MQ=255)
                                                                                     ggCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACAt                                                                                            >  1:1153004/1‑113 (MQ=255)
                                                                                     ggCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACAt                                                                                            <  2:1153004/113‑1 (MQ=255)
                                                                                        gTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACTGCCATTGGGCGACGACCATGCCAACTTTTCACCACATc                                                                                           <  2:1827878/111‑1 (MQ=255)
                                                                                        gTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATc                                                                                           >  1:1827878/1‑111 (MQ=255)
                                                                                           aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcga                                                                                                                     >  1:1366459/1‑82 (MQ=255)
                                                                                           aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcga                                                                                                                     <  2:1366459/82‑1 (MQ=255)
                                                                                           aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAGCAAGAGGAAGCGGTTCGACGCTCTCCAGCAGCATActtc                                                  >  1:1078026/1‑149 (MQ=255)
                                                                                            aaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAAGAAGAGGAAGCGGTTCGACGCTCTCCAGCAGCATActtct                                                 >  2:1716271/1‑149 (MQ=255)
                                                                                            aaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGACGGCGCATTAACTCCCCGTTCCTACAGCCACTGGGCGACGACCATGCCCACTTTTCACCACATCAACAAGAGGACGCGGTTCAACGCTATCCAGCAGCATCcttct                                                 >  1:1096610/1‑149 (MQ=255)
                                                                                            aaaTAATGCCGTAATCCCAGGGGCGCCAGCAATGCCTCAGCGATGGCGCTTTATCTCCCCGTTCCTACAGCCATTGAGTGACGTTAAAGCGAGCTTTTTTTCCTATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtct                                                 <  2:1492055/149‑1 (MQ=255)
                                                                                              ataatGGCGTAATCCCAGGTGCNCCANCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa                                               <  1:1962649/149‑1 (MQ=255)
                                                                                              ataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa                                               <  1:352730/149‑1 (MQ=255)
                                                                                              ataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAAGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa                                               <  1:1959505/149‑1 (MQ=255)
                                                                                               taatGGCGTAACCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTAc                                              <  2:1439937/149‑1 (MQ=255)
                                                                                                   ggCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCTAGCAGCATACTTCTTACTGCt                                          >  2:217477/1‑149 (MQ=255)
                                                                                                   ggCGTAAACCCACGCGCGACAGCAATGATTCGGCGATGGCGCTTTAACCTCCCGTTCCTACAGCCATTGGGCGACGACCCTGCCTCCTTTTCACCACATCAGCCGGAGGAAGCGGTTCGACTCTATCCAGCAGCATACTTCTTACTGCt                                          <  2:340235/149‑1 (MQ=255)
                                                                                                    gCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCtt                                         >  1:1720181/1‑149 (MQ=255)
                                                                                                    gCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATAATTCTTACGGCtc                                         >  1:268769/1‑148 (MQ=255)
                                                                                                        agacacAGGTGCGCCGGCAATGCCTCGGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGt                                     <  2:701703/144‑1 (MQ=255)
                                                                                                          tCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttt                                   <  2:1689743/149‑1 (MQ=255)
                                                                                                           cccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttt                                  <  2:368467/149‑1 (MQ=255)
                                                                                                            ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCAAATACACGAGGGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttttt                                 >  1:2040286/1‑149 (MQ=255)
                                                                                                            ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACGCCACGTTCCAACAGCAATTGGGCGACGCCCATGCCAACTTTTCACCACATCAACACCAGAACGCGGAACGACCCTACCCAGCAGCATACTACTTTCTGGTCCTGttttt                                 >  2:1455653/1‑149 (MQ=255)
                                                                                                             cAGGGGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttttt                                <  2:743401/149‑1 (MQ=255)
                                                                                                               ggTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCa                              <  1:669710/149‑1 (MQ=255)
                                                                                                                gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCTAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAc                             <  1:217477/149‑1 (MQ=255)
                                                                                                                gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAc                             >  2:1203692/1‑149 (MQ=255)
                                                                                                                gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAc                             <  1:100776/149‑1 (MQ=255)
                                                                                                                  gtgcCAGCAATGCCGCAGCGAGGGCGCTTTAACTCCCCTGTCCTACCGCCATTTGTCGCCGACCATGTCAACTTTTCACCACATCAACAAGATGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                           <  1:1329980/147‑1 (MQ=255)
                                                                                                                  gcgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                           >  1:1179002/1‑149 (MQ=255)
                                                                                                                  acgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                           <  2:1258654/148‑1 (MQ=255)
                                                                                                                    gcCAGCAATGCCTTAGCGGTGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACgcgc                         <  2:1542194/149‑1 (MQ=255)
                                                                                                                    gcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                  <  2:366801/76‑1 (MQ=255)
                                                                                                                    gcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                  >  1:366801/1‑76 (MQ=255)
                                                                                                                    gcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACgcgc                         >  1:325291/1‑149 (MQ=255)
                                                                                                                     ccAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCt                        >  2:2031901/1‑149 (MQ=255)
                                                                                                                     ccAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCt                        >  1:1040546/1‑149 (MQ=255)
                                                                                                                      cAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCaa                                                                                                         <  1:1031519/67‑1 (MQ=255)
                                                                                                                      cAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCaa                                                                                                         >  2:1031519/1‑67 (MQ=255)
                                                                                                                      cAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCCCGCGCTa                       >  2:1857003/1‑149 (MQ=255)
                                                                                                                        gCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcacca                                                                                               >  1:862494/1‑75 (MQ=255)
                                                                                                                        gCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcacca                                                                                               <  2:862494/75‑1 (MQ=255)
                                                                                                                        gCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTTTGTTTTTTCACGCGCTAAc                     <  2:1760150/149‑1 (MQ=255)
                                                                                                                           aTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGACGCTTTTCGCCCTCAGACTGCGGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCa                  <  1:497486/149‑1 (MQ=255)
                                                                                                                                tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCCACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg             >  2:79994/1‑149 (MQ=255)
                                                                                                                                tCAGCGATGGCCCTTTAACTCCCCGTTCCTACAGCCATTGGTCGACGACCATGCCACCTTTTCCCCACATCCCCAAGAGGAAGCGGCTAGACCCTATCAAGCACCGTACTTGTTACTGCGTCTGTTTTTTCTCGTGCTTACGTCTGCgt             >  2:410577/1‑148 (MQ=255)
                                                                                                                                tCAGCGATGGAGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTCCTTACTGCTTCTGTTATTTCACGCGCTAACGCATGCgg             >  1:2030654/1‑149 (MQ=255)
                                                                                                                                     gATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGa                                                                       <  2:107008/86‑1 (MQ=255)
                                                                                                                                     gATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGa                                                                       >  1:107008/1‑86 (MQ=255)
                                                                                                                                         gcgcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATc    <  1:1340941/149‑1 (MQ=255)
                                                                                                                                           gcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAGCgg  >  1:658533/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGG  >  NC_000913_3_pae_tpiA/2817027‑2817313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: