Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,111,596 G→A L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,111,5960GA98.0% 302.1 / NA 102L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (57/42);  minor base C (1/1);  total (58/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATC  >  NC_000913_3_pae_tpiA/4111451‑4111741
                                                                                                                                                 |                                                                                                                                                 
aaaGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTATGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAccc                                                                                                                                                <  2:851893/149‑1 (MQ=255)
   ggAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTCTGCTTTTTGTCATTTATTAACACGCCCAAGCGATTCAGATAGCGCCCGCTTAATCGGTTCAACAGCGAAGGTCGACCCCtt                                                                                                                                             >  1:487249/1‑149 (MQ=255)
     aaGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCtttt                                                                                                                                           >  1:1303396/1‑149 (MQ=255)
         ctgctgccCTGCGGGGCGGCAGGTCTGTGAAACAGTATAGAGATGCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGCTTCAGATAGCGCCAGCTGAATCGGTTCAACAGACAAGATCAACCCCTTTTGGca                                                                                                                                       >  2:599406/6‑148 (MQ=255)
          ggCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCACAAGCAAAGGACCACCCCTTTTCGCCg                                                                                                                                      >  2:527368/1‑149 (MQ=255)
           gccgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACACCGAAGGTCAACCCCTTTTCGCCGt                                                                                                                                     >  2:1152971/1‑149 (MQ=255)
            ccgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGtt                                                                                                                                    <  2:1357181/149‑1 (MQ=255)
             cgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGGGCAAGCTTAATCGGTTCAACAGCGACGGTCAACCCCTTTTAGCCGGTg                                                                                                                                   >  1:441243/1‑149 (MQ=255)
              gccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGt                                                                                                                                  >  1:1292016/1‑149 (MQ=255)
                 ccGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTTTTTTTTTTTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCACCCCCTTTTCTCCGTTGTCcg                                                                                                                               >  2:95901/1‑149 (MQ=255)
                  cGCAGGGCAGCCGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAACACTTTTCGCCGTTGTCcgc                                                                                                                              >  1:1320378/1‑149 (MQ=255)
                  cGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgc                                                                                                                              >  1:1703161/1‑149 (MQ=255)
                  cGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgc                                                                                                                              >  2:1112983/1‑149 (MQ=255)
                    cAGGGCAGCAGGTCTGTGAAACAGTATAGAGATGCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGa                                                                                                                            <  2:1836001/149‑1 (MQ=255)
                       ggCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacaa                                                                                                                         >  1:1339390/1‑149 (MQ=255)
                       ggCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacaa                                                                                                                         <  1:540948/149‑1 (MQ=255)
                        gcagcaGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACGGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacaac                                                                                                                        >  2:926544/1‑149 (MQ=255)
                        gcagcaGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacaac                                                                                                                        <  2:467368/149‑1 (MQ=255)
                          agcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACAt                                                                                                                      >  1:540422/1‑149 (MQ=255)
                            cagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCAGCGACGACATaa                                                                                                                    >  2:1862110/1‑149 (MQ=255)
                                  tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACGGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                              <  1:926544/149‑1 (MQ=255)
                                  tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGt                                                                                                                                     >  2:760648/1‑126 (MQ=255)
                                  tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGt                                                                                                                                     <  1:760648/126‑1 (MQ=255)
                                  tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcg                                                                                                                               >  2:1321619/1‑132 (MQ=255)
                                  tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcg                                                                                                                               <  1:1321619/132‑1 (MQ=255)
                                   gtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTg                                                                                                             >  1:403575/1‑149 (MQ=255)
                                   gtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTg                                                                                                             <  2:172460/149‑1 (MQ=255)
                                    tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAcc                                                                                                                                                 <  2:133147/112‑1 (MQ=255)
                                    tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGCCCACATAACGCATTGc                                                                                                            >  1:647061/1‑149 (MQ=255)
                                    tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGc                                                                                                            >  1:1697275/1‑149 (MQ=255)
                                    tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATGCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAcc                                                                                                                                                 >  1:133147/1‑112 (MQ=255)
                                      aaaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTAAAACTTGCATTCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAc                                                                                                          >  1:1124958/1‑149 (MQ=255)
                                        aCAGTATAGCGATTCATCGGCACAAAGGCTTTGCTTTTTATCAGTCATTCAAACCTTCAAGCGATTCTGATTGCGCCAGCTTAATCGGTTGAACAGTGAAGGTCAACCCCGCTTCGCCGAAGTCCGCGACAACATAACGCAGTGCATCt                                                                                                        <  1:250027/149‑1 (MQ=255)
                                           gTATAGGGATTCATCGGCACAAAGGCTGTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCt                                                                                                     <  2:703244/149‑1 (MQ=255)
                                               agagATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCCGTGCACCTTCTGtcc                                                                                                 >  2:750944/1‑148 (MQ=255)
                                               agagATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAACCCATTGTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtct                                                                                                 >  2:750446/1‑149 (MQ=255)
                                                   aTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGCCAACCTAACGCAGTGCACCTTCTGTCTCGgt                                                                                             >  1:2029366/1‑149 (MQ=255)
                                                   aTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgt                                                                                             >  1:1605469/1‑149 (MQ=255)
                                                     tCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgtgt                                                                                           >  2:1195665/1‑149 (MQ=255)
                                                     tCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgtgt                                                                                           >  2:373110/1‑149 (MQ=255)
                                                       aTCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCACGCGATTCAGATAGCGCCAGCTTAATCGGTTCACCAGCGAAGGTCAACCCCTTTTCGCAGTTGTCCGCGACAACATAAAGCCGTGCACCTTCTGTCTCGGTGtaa                                                                                         >  1:1208074/1‑149 (MQ=255)
                                                             acaAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgt                                                                                   >  1:1414088/1‑149 (MQ=255)
                                                                aaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCCGTGCACCTTCTGTCTCGGTGTACTCACgtttg                                                                                >  2:1626069/1‑149 (MQ=255)
                                                                 aGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgtttgt                                                                               <  1:1460465/149‑1 (MQ=255)
                                                                      ttgcttcTTGCCATTTCTTCACCCCTCCAAGCGATTCAGCTCGCGACAGCTTAATCGGTTCCCCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTc                                                                          <  1:1994794/149‑1 (MQ=255)
                                                                          tttttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATCACGATTGTTTTTCCccg                                                                      >  1:620221/1‑149 (MQ=255)
                                                                          tttttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccg                                                                      <  1:57962/149‑1 (MQ=255)
                                                                            gttGTCATTTATTCAAATCCTTAGGCGGTTCGGGTTTGGTCTTTTTAATCGGTTCAACTGCGAAGGTCAATCCCTTTTGGCCGGTTTCCGCGATAACATAGCGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccgcc                                                                    <  2:155425/148‑1 (MQ=255)
                                                                             ttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGATTAACGTGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAACAACGTTTGTTTTTCCccgccg                                                                   <  1:1214452/149‑1 (MQ=255)
                                                                                tCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTta                                                                >  2:607157/1‑149 (MQ=255)
                                                                                  aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTGAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                              >  1:1762550/1‑149 (MQ=255)
                                                                                    ttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagtag                                                            >  2:378617/1‑149 (MQ=255)
                                                                                       ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGa                                                         <  1:944463/149‑1 (MQ=255)
                                                                                        tCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATATCCCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                        <  1:68904/149‑1 (MQ=255)
                                                                                        tCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCgcc                                                        >  1:455552/1‑147 (MQ=255)
                                                                                          aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTc                                                                                                                                          >  1:974056/1‑65 (MQ=255)
                                                                                           aatcTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGc                                                     <  1:1134920/146‑1 (MQ=255)
                                                                                             ccTTCAAGCGATTCAGAAAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAg                                                   <  1:804356/149‑1 (MQ=255)
                                                                                               ttCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                 >  1:2071846/1‑149 (MQ=255)
                                                                                                tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttt                                                <  1:1301374/149‑1 (MQ=255)
                                                                                                 cAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTGTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTGTTTCCCCGCCGTTAGTAGCGACTGCAGtttt                                               <  1:142924/149‑1 (MQ=255)
                                                                                                  aaGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttttt                                              >  1:1425383/1‑149 (MQ=255)
                                                                                                  aaGCAATTCAAATAGCGCCAATTTAATCGATTCAACAGCGATGGTCAATTCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttttt                                              <  2:1563956/149‑1 (MQ=255)
                                                                                                   aGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAAGGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAgacttgt                                             >  2:1475917/1‑143 (MQ=255)
                                                                                                   aGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtttttt                                             >  2:695862/1‑149 (MQ=255)
                                                                                                   aGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGACGTTAGTAGCGACTGCGGtctttt                                             >  2:1883626/1‑149 (MQ=255)
                                                                                                    gtgaatCAGTTAGCGCCTTCATAATCAGTTCAACTTCGAATGTCGACACTTTTTCGACGCTGTCCGCGACAACATAACGCACTGCTCCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                            <  2:2042378/144‑1 (MQ=255)
                                                                                                     cgaaacAGATAGCGACAGTCTACTGGGTTCAGCAGTGAAGGTTTGCCCTTTGTTGCAGTTGTCCGCGAGTACAGAACGCAGTGCCCTTTCTGTCTCGGTGGAATAACGTTTGTTTTTCCCCGCCGTGAGTAGCGACTGCAGTTTTTTCt                                           <  2:1975291/144‑1 (MQ=255)
                                                                                                     cGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTc                                                                                                      >  2:56583/1‑90 (MQ=255)
                                                                                                     cGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTc                                                                                                      <  1:56583/90‑1 (MQ=255)
                                                                                                     cGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCt                                           <  1:378617/149‑1 (MQ=255)
                                                                                                      gATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTg                                          <  1:913677/149‑1 (MQ=255)
                                                                                                       aTTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTATCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTgg                                         >  1:1973003/1‑149 (MQ=255)
                                                                                                           agttAGCGCCAGCTTAATCGGTTCAACAGCTAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCttt                                     <  1:1768563/146‑1 (MQ=255)
                                                                                                           aGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCTACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTTGCttt                                     >  1:1640979/1‑149 (MQ=255)
                                                                                                            tgactagcCAGCTTTAGCGGGCCAACAGCGAAGGGCAACCCCTTTTCGCCGGTGTCCGCGACAACATAACGCAGTGCACCTTCTCTCTCGGGGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtg                                    <  2:803279/143‑1 (MQ=255)
                                                                                                             aTAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGAAATAACGTTTGTTTTTCCCCGCTGTTAGTAGCGCGTGCAGTTTTTTCTTGCTTtgt                                   >  1:962226/1‑149 (MQ=255)
                                                                                                             aTAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCCATTCGCCGTTGTCCACGACAACATAACACAGTGCACCTTCTTGCTCGGCGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                   <  1:610851/149‑1 (MQ=255)
                                                                                                                gcgcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtt                                <  1:811581/149‑1 (MQ=255)
                                                                                                                  gcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt                              >  2:1877315/1‑149 (MQ=255)
                                                                                                                   ccAGGTTAATCGGTTCAACAGGGAATGTCACCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                             <  2:1640979/149‑1 (MQ=255)
                                                                                                                   ccAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                             <  1:1112983/149‑1 (MQ=255)
                                                                                                                   ccAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                             <  1:655680/149‑1 (MQ=255)
                                                                                                                     aGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                           >  1:1694330/1‑149 (MQ=255)
                                                                                                                       cTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                         <  1:2055413/149‑1 (MQ=255)
                                                                                                                        ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                        <  2:1425383/149‑1 (MQ=255)
                                                                                                                        ggaaTCGGTGCAACAGCGAACGTCCACCCCTTTTCGCGGTTTTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGAGCGCTCAGTGTTTTCTGGCTTTGTGTTTTGGTCAt                        <  2:455552/147‑1 (MQ=255)
                                                                                                                           aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTTCACCTTCTGTCGCGGTGTACTACCGCTTATTTCACCCCGCCGGTAGTAGCGCCTGCAGTTTTATCTGCCTTTTTGTTTTGGTCCTTat                     >  2:1387829/1‑148 (MQ=255)
                                                                                                                           aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTGTTGGTCATTaa                     >  1:1518214/1‑149 (MQ=255)
                                                                                                                              ggTTCAACAGCGAAGGTCAACCCCTGTTCGCCGTTGTCCGCGCCAACACACCGCAGAGCACCTACTGTCTCGGTGTAATAACGTTTGTTTTTCCCCCCCGTTAGTAGCGCCAGCAGATTCTTCTGCCATTGTGCTTTGGTCCGTAATgg                  >  2:1203461/1‑149 (MQ=255)
                                                                                                                                 tCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt               <  2:1605469/149‑1 (MQ=255)
                                                                                                                                   aaCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                              >  1:819494/1‑100 (MQ=255)
                                                                                                                                   aaCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                              <  2:819494/100‑1 (MQ=255)
                                                                                                                                    aCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTCTGTGGTCTGGTCATTAATGGGGTGAg            >  1:274128/1‑149 (MQ=255)
                                                                                                                                    aCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACGGGAGTTTTTTCTGGCGTTGTGTTTTGGTCATTAATGGGGTGAg            >  1:1231445/1‑149 (MQ=255)
                                                                                                                                       gCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATgg                  >  2:575683/1‑140 (MQ=255)
                                                                                                                                       gCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATgg                  <  1:575683/140‑1 (MQ=255)
                                                                                                                                             gTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTCATGGGGTGAGTGTGCGGAt   >  2:553592/1‑149 (MQ=255)
                                                                                                                                             gTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAt   <  1:1877315/149‑1 (MQ=255)
                                                                                                                                              tCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGGTc  >  1:727309/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATC  >  NC_000913_3_pae_tpiA/4111451‑4111741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: