Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_vch_tpiA	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_vch_tpiA	1299499–1300697	1300697	1–1199	60 [0]	[0] 59	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_tpiA	= 1299498	0 (0.000)	58 (1.260)	48/278	0.0	100%	noncoding (1299498/4641682 nt)	repeat_region	repeat region
	?	NC_000913_3_vch_tpiA	1300698 =	0 (0.000)					noncoding (1300698/4641682 nt)	repeat_region	repeat region

TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_vch_tpiA/1299351‑1299646                                                                                                                                                    |                                                                                                                                                     atacccaaTTACAGTGAATTAACAGGGTTTCCTTAACTGCTTTTCCTCAACAGCATGATATTGTCGCCACATCATAACCCTGGGCTTGCGGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTTCTaaa                                                                                                                                                     <  2:151794/143‑2 (MQ=255)   ctcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCTCCACATCATAATCCTGAGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                                                                                                                   >  2:690092‑M1/1‑146 (MQ=255)    tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTCCCTTTGTTAGAGGAAAGTGCTAAttaa                                                                                                                                                  >  2:259612‑M1/1‑145 (MQ=255)      aaaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                                <  1:262489‑M1/149‑7 (MQ=255)         ttACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                                                                                                                             <  1:545624‑M1/149‑10 (MQ=255)            tagttagtgaTAAGGGTTTCCTTAACTGCTTCTCCTCCCCATTATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCTATTGCTTTTTGTTGTCGAAAGTGCTAAataataatcaat                                                                                                                                          <  2:96773‑M1/148‑13 (MQ=255)              gttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattg                                                                                                                                        >  2:129125‑M1/1‑135 (MQ=255)                  gttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                                    >  1:504848‑M1/1‑131 (MQ=255)                      taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                                                                                                                                >  2:756635‑M1/1‑127 (MQ=255)                       aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCGCCACCATAATCCTGGGCTTGCTCAAGAATAATTCAAATGATATTATTAATTCCACTGCCTTTGATAGAGGAAAGTGCTAAataataatcaattgttcaattat                                                                                                                               >  2:304286‑M1/1‑126 (MQ=255)                         ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattattaaattattg                                                                                                                             >  2:266617‑M1/1‑124 (MQ=255)                                 ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttc                                                                                                                     >  1:224389‑M1/1‑116 (MQ=255)                                  tAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                                    <  2:737834‑M1/149‑35 (MQ=255)                                         ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactcg                                                                                                             >  1:663784‑M1/1‑108 (MQ=255)                                            tcctcACCAACATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaac                                                                                                          >  2:200489‑M1/1‑105 (MQ=255)                                             cctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                         >  1:786088‑M1/1‑104 (MQ=255)                                                  ccttcatgttATTTTCGCCACATCATAATTCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGTTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                    <  2:317998‑M1/146‑51 (MQ=255)                                                   catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                                   >  2:565703‑M1/1‑98 (MQ=255)                                                   catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatggttaaattgttgtacatttcactactggaactgtaatc                                                                                                   >  1:273428‑M1/1‑98 (MQ=255)                                                          ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattttgcatttcactactggaactgtaataagaaaag                                                                                            >  1:508372‑M1/1‑91 (MQ=255)                                                          ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                            >  2:170265‑M1/1‑91 (MQ=255)                                                             ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataataaattattaaattattgtgaacatcactactggaactgtaagcagaaaagata                                                                                         >  1:17791‑M1/1‑88 (MQ=255)                                                                   ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatctgagaagatagacatg                                                                                   >  1:1080‑M1/1‑82 (MQ=255)                                                                   ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCAACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattcttatgcagttcactactggaactgtagtcagaaaagatagacatg                                                                                   >  1:298797‑M1/1‑82 (MQ=255)                                                                    cacaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCGACTGACTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggcactgtaatcagatacgatagacaggc                                                                                  >  1:545682‑M1/1‑81 (MQ=255)                                                                        tcatAAGCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                              <  2:469229‑M1/149‑73 (MQ=255)                                                                              tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                                                                        >  1:793197‑M1/1‑71 (MQ=255)                                                                                   ggCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatccattgttaaattattgtgcatttcactactggaactgtaattagaacagatagccatgtttcaccaatatctat                                                                   >  1:380788‑M1/1‑66 (MQ=255)                                                                                       tGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttga                                                               <  1:522636‑M1/149‑88 (MQ=255)                                                                                           gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattga                                                           <  2:54277‑M1/149‑92 (MQ=255)                                                                                            aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                          <  1:129125‑M1/149‑93 (MQ=255)                                                                                            aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                          >  1:365443‑M1/1‑57 (MQ=255)                                                                                               aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgaaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattg                                                       >  2:630829‑M1/1‑54 (MQ=255)                                                                                                    ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattcaaata                                                  >  2:485318‑M1/1‑49 (MQ=255)                                                                                                       aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgt                                               >  1:629571‑M1/1‑46 (MQ=255)                                                                                                           gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                           >  1:4495‑M1/1‑42 (MQ=255)                                                                                                            atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                          >  2:410098‑M1/1‑41 (MQ=255)                                                                                                            atatCATTAATTTCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                          <  2:329891‑M1/149‑109 (MQ=255)                                                                                                                tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtgatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                      >  2:667702‑M1/1‑37 (MQ=255)                                                                                                                tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                      >  1:691092‑M1/1‑37 (MQ=255)                                                                                                                   taATTCCACTTCCTGTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                                   <  1:162481‑M1/149‑116 (MQ=255)                                                                                                                    aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatg                                  <  2:4495‑M1/149‑117 (MQ=255)                                                                                                                     aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                                 >  2:601975‑M1/1‑32 (MQ=255)                                                                                                                      ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataaacaatggctaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgga                                <  1:572427‑M1/149‑119 (MQ=255)                                                                                                                       tCCACTGCCTTTGGTAGAGGAAAAGGCTAAatcatgatcagtaggtagattgtggtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                               <  2:504848‑M1/149‑120 (MQ=255)                                                                                                                         cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgc                             >  1:292185‑M1/1‑28 (MQ=255)                                                                                                                           ttgCCTTTGGTATAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                           <  1:641237‑M1/148‑124 (MQ=255)                                                                                                                           cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                           <  2:786088‑M1/149‑124 (MQ=255)                                                                                                                                tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                      <  1:1757‑M1/149‑129 (MQ=255)                                                                                                                                tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                      >  1:63539‑M1/1‑21 (MQ=255)                                                                                                                                tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaaatgtaatcagaaaagatagacatgcttagccaatctctatttgatggaattgaaagatgtttgttaaggcatggatgcaagcgat                      >  2:463424‑M1/1‑21 (MQ=255)                                                                                                                                    gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat                  >  2:356784‑M1/1‑17 (MQ=255)                                                                                                                                    gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat                  >  2:458598‑M1/1‑17 (MQ=255)                                                                                                                                    gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                    <  1:803927‑M1/83‑67 (MQ=255)                                                                                                                                    gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                    >  2:803927‑M1/1‑17 (MQ=255)                                                                                                                                          gAAACTGTTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat            <  1:231178‑M1/149‑139 (MQ=255)                                                                                                                                            aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatac          <  1:565703‑M1/149‑141 (MQ=255)                                                                                                                                               tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt       >  2:570734‑M1/1‑6 (MQ=255)                                                                                                                                               tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt       <  1:539364‑M1/149‑144 (MQ=255)                                                                                                                                                    aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatatgagtaaattgaaagatgtttgttaaggcattgatgcaagctatagattctgatacggtcaata  >  2:92141‑M1/1‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_vch_tpiA/1299351‑1299646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑