Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_vch_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_vch_tpiA 1299499–1300694 1300697 4–1199 33 [0] [0] 33 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_tpiA = 12994980 (0.000)32 (1.040) 29/280 0.2 100% noncoding (1299498/4641682 nt) repeat_region repeat region
?NC_000913_3_vch_tpiA 1300698 = 0 (0.000)noncoding (1300698/4641682 nt) repeat_region repeat region

TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCC  >  NC_000913_3_vch_tpiA/1299351‑1299645
                                                                                                                                                   |                                                                                                                                                   
ttctCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                                    <  2:236546‑M1/149‑2 (MQ=255)
   tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                                 >  2:352873‑M1/1‑145 (MQ=255)
       aTTACAGTTATTTATAAGTATTTCCTTAACTGCTCCTCCTCACCATCATGTTATTTTCTCCACATCATAATCCTGGGCTTTCTGAAGAATAATTTAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                                                                                                                             <  1:206807‑M1/149‑9 (MQ=255)
          aCAGTTAGTTATAAGGATTTTCTTAACTGCTTTTCCTTACCATCATGTTATTTTCGCCACATCATAATCCTTGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                          <  1:149923‑M1/149‑12 (MQ=255)
            agttagttATAAGGATTTCCTTAACTGCTTCTCCTCCCCATCATTTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                        <  2:134010‑M1/149‑14 (MQ=255)
                     taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATGTTCGTGGTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                                                                                                                               >  1:264903‑M1/1‑127 (MQ=255)
                     taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGACTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                                                                                                                               >  1:233183‑M1/1‑127 (MQ=255)
                                 tAACTGCTTCTCCGCACCATCATGTTATTTTCGCCACAACATAATCCTGTGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                                   <  2:529714‑M1/149‑35 (MQ=255)
                                           tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTACACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttacattattgtgcatttcactacttgaac                                                                                                         >  1:35452‑M1/1‑105 (MQ=255)
                                                       tGTTATTTTCGCCACATCATAATCCTGGGCTTGCGGAAGAATAATTGAAATGATATTATTAATCCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                             <  1:492471‑M1/149‑57 (MQ=255)
                                                            ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                        >  2:537953‑M1/1‑88 (MQ=255)
                                                              ttCGCTAGATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcataaaagataga                                                                                      <  2:104351‑M1/149‑64 (MQ=255)
                                                                    acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTACTAAataataatcaattgttaaattattgtgaatttcactactggaactgtaatcagaaaagatagacatgct                                                                                >  1:128620‑M1/1‑80 (MQ=255)
                                                                        catAATCCTGGGCTTGGTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                            <  2:95160‑M1/149‑74 (MQ=255)
                                                                                  ggCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                                  <  1:352873‑M1/149‑84 (MQ=255)
                                                                                  ggCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagacaatctctat                                                                  >  1:299407‑M1/1‑66 (MQ=255)
                                                                                           aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattttactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                         <  1:333696‑M1/149‑93 (MQ=255)
                                                                                           aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                         <  1:329557‑M1/149‑93 (MQ=255)
                                                                                           aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGATAAAGGAAATTGCTAAataataatcaattgttacattatttttcatttcactactggaactgtaatcagaaaagatagacatgattagcatatctctatttgatgtaa                                                         >  2:367328‑M1/1‑57 (MQ=255)
                                                                                                        aagatATAAATAATTCCAACGCCTATAGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaaaatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                            <  2:224711‑M1/147‑106 (MQ=255)
                                                                                                        aTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcaaaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                            <  1:106562‑M1/149‑106 (MQ=255)
                                                                                                          gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatacttagcaaatctctatttgattgaattgaaagatgtttgt                                          >  2:97962‑M1/1‑42 (MQ=255)
                                                                                                                attaAGTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggc                                    <  2:34208‑M1/149‑114 (MQ=255)
                                                                                                                 ttaATTCCACTGCGTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                                   <  2:206740‑M1/149‑115 (MQ=255)
                                                                                                                 ttaATTCCACTGCCTTTCGTAGAAGAAAGTGCAAAataataatcaattgttaaattattgtgcatttaactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                                   >  2:547651‑M1/1‑35 (MQ=255)
                                                                                                                    aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattaaattgaaagatgttttttaaggcatgg                                >  2:485962‑M1/1‑32 (MQ=255)
                                                                                                                          cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattaaaatacaaataactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                          >  1:141946‑M1/1‑26 (MQ=255)
                                                                                                                          cTGACTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                          <  2:233183‑M1/149‑124 (MQ=255)
                                                                                                                                ttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata                    <  2:430602‑M1/149‑130 (MQ=255)
                                                                                                                                     agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcacgcaagagattc               >  2:162768‑M1/1‑15 (MQ=255)
                                                                                                                                     agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc               <  1:264036‑M1/149‑135 (MQ=255)
                                                                                                                                              tGCTAAataataatcaattgttacataagtttgcattttaattccagacgtgtgatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt      <  1:540372‑M1/149‑144 (MQ=255)
                                                                                                                                                  aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaat  <  1:72183‑M1/149‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCC  >  NC_000913_3_vch_tpiA/1299351‑1299645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.