| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 4,296,090 | 0 | C | T | 22.5% | 54.1 / 14.5 | 41 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (20/11); new base T (5/4); total (26/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA > NC_000913_3_vch_tpiA/4295945‑4296230 | ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc > 2:311660/1‑149 (MQ=17)ggAGAAGGCGTGTAGGCCGCAGGCGACATGAACGCCTGGTGCGACGCTTAACGCGTCTGATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc < 2:375990/149‑1 (MQ=11) aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCTTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCtt < 1:223018/149‑1 (MQ=255) gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGc > 1:363675/1‑149 (MQ=18) aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt < 1:311660/149‑1 (MQ=21) tCCGACATCAACGCCTGATGTGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc < 1:55067/149‑1 (MQ=255) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc > 1:489233/1‑149 (MQ=37) cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc > 1:410470/1‑149 (MQ=25) cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg > 2:373113/1‑149 (MQ=38) cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:520213/149‑1 (MQ=39) aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc > 2:136073/1‑149 (MQ=21) cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc < 1:52497/149‑3 (MQ=16) cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATatc > 1:466976/1‑147 (MQ=12) cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg > 1:231397/1‑149 (MQ=37) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCATGATTTTGTAGGTCAGAAAAGACGTTTACGCCGCATCCGACATCAACGCGTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTAcac > 1:140715/1‑147 (MQ=11) gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCTTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa < 1:323900/149‑1 (MQ=38) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTGGGTCGAATAAGGAGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCCGCGCa > 1:267063/1‑149 (MQ=11) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGACAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAAACAGTGCa > 2:352811/1‑149 (MQ=255) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTGGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTCAGCCAGACAGCGCAATAGCCTAAt > 1:117178/1‑149 (MQ=11) ttATCAGGCCTACGCCAGACAGCGCAATACCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAGGCGACGCTAGCCGCGTCTTATCAGGCCTCCGCACGCCAGCCAAACAGCCtaca > 2:221851/1‑146 (MQ=11) aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTAATGCGACGCTTGCCGCGTCTTATCAGGCCTACTCCAGACAGCGCACTAGCGTGATTTatc > 1:111743/1‑147 (MQ=255) aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa < 2:489233/149‑1 (MQ=38) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCAGCCTCCCACATCAACGCCTGATGCGACGCTTGCCGCGTATTATCAGGCCTACGCCATCCAGTCCAATAACGTGATTTAGCGGGATTTTGTAg > 1:47146/1‑149 (MQ=11) aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATa > 2:292740/1‑149 (MQ=38) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGc < 1:192745/149‑1 (MQ=37) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGGCTTATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTCACGCAATTCTGTCGATCGGATAAGCCgc > 2:73043/1‑148 (MQ=11) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCCCATCCGTCTTAAGCGGATCAGTCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt < 1:542724/149‑1 (MQ=11) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTGCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAACCTGATTTAGCGTAACTTTACAGGTCGAACAAGGCGtg > 1:266628/1‑148 (MQ=11) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATCAACGCCTGATGCTACGCTTGCCGCGTCTTAGAAGGCCTACTCCAGCCAGCGCAATAGCCTGATTTAGCGCGATTTTGTAGGTCGTGTCAGGCGtt > 1:500191/1‑149 (MQ=11) gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCACGTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCCGATAAGGCGCTTATGCCGCATCCGACac > 2:298150/1‑148 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGc > 2:343472/1‑136 (MQ=40) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGc < 1:343472/136‑1 (MQ=40) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCAGATAGGGCGTTTATGCCGCAGCCGACATCAAc > 2:3689/1‑149 (MQ=255) ggCGTTTACGCCGCATCCGCTATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTTGTCTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc < 1:106072/149‑1 (MQ=11) ggCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt < 2:102001/43‑1 (MQ=255) ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCtt > 1:102001/1‑43 (MQ=40) gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATGCCAGACAGTGCAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc < 1:78894/149‑1 (MQ=255) ccgcATCCGACATCAACGCCTGATGCGACCCTTGCCGCGTCTTATCAGGCGTACGCCCGACAGCGCACTAGCCTGATTTAGCGTGAGTTTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGAGATCAACGCCTGATGCGACGTGTAAc > 1:466930/1‑149 (MQ=11) cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCAGACAGACAGCGCAATAACCTAATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAAcg > 1:47178/1‑149 (MQ=11) gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc < 1:373113/149‑1 (MQ=35) gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa > 2:102880/1‑149 (MQ=35) | GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA > NC_000913_3_vch_tpiA/4295945‑4296230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |