Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA2,679,5250GT100.0% 43.5 / NA 18A1074D (GCT→GAT) yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/10);  total (8/10)

TAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGA  >  NC_000913_3_vch_tpiA/2679379‑2679655
                                                                                                                                                  |                                                                                                                                  
tAGGGTTTTATTTAACAACGGCTTATTCTAATTATGTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTTATAGGGTTTAGTGATCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATcc                                                                                                                                  <  1:150927/149‑1 (MQ=255)
          attaACAACGGCTTATTCTAATTATTTTGGGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCg                                                                                                                        >  2:500316/1‑149 (MQ=255)
                  cGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAt                                                                                                                <  1:349761/149‑1 (MQ=255)
                    gCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCACTCCGGATTAATCGCCTTCatcta                                                                                                              >  2:404290/1‑145 (MQ=255)
                     ttgaTTCTAACTCTGGTGTGTTGGGCGGCGGTGCGTGCGGTGCAAAGGTTACAGTTACTAGAGTTTGTTAAGCGCGGTTAAAAATAAAAGTTCTCACGGTGGTGAATAATGCCGCTTTCGGTCAATCCGGATTAAACGCCGTCAATTCa                                                                                                             <  2:68422/146‑1 (MQ=255)
                           ttAATTATTTTGTGATGAGCGGTAGCACGTGACGATCAACTATTACAGTTTATAGTGTTTAGTGGACGCGGTTAAAAATAAGAGGCATCACGGTGGTGAATACTGCCGCTTTCGGCCAATCCAGATTAAGCGCCGTCAATTCAGCACGa                                                                                                       <  2:258721/148‑1 (MQ=255)
                                             gCGGAAGCGCGTGCAGCGAAAAGATTAAGGTTATGAGGGCTTAGTGAATGCGGGTAAAAGTGAGAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACGCATATTACGCa                                                                                     >  2:408962/1‑149 (MQ=255)
                                                                               tAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCAAGCATCGcca                                                   >  1:344767/1‑149 (MQ=255)
                                                                                          gAACGCGGTTAAAAATAAAACACATCCTGCTTGTGAAAAATCCCGCGTACGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGCCTCATATTGCGCAACCTCCCCTCGTCCCGCGGACGCCAGCATCGCCACCGTCAGGCAt                                        >  2:200537/1‑149 (MQ=255)
                                                                                            aCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCACCCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAttt                                      <  1:500316/149‑1 (MQ=255)
                                                                                                  ttAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCttt                                <  1:447893/149‑1 (MQ=255)
                                                                                                                  tCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCTCGGACGCCAGCATCGCCACCATCAGTCATTTTTCTTTATGCTGCTGTTGTaaa                <  1:185998/149‑1 (MQ=255)
                                                                                                                    aCGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATc              <  1:445142/149‑1 (MQ=255)
                                                                                                                           tgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGACTTTTTCTTTATGCTGCTGTTGTAAATCGCCCTAc       >  2:270290/1‑149 (MQ=255)
                                                                                                                            gAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCCTCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATAcc      >  2:310164/1‑148 (MQ=255)
                                                                                                                                aatGACGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGTCTCATATTGCGCAACCTCCCCGAGTCCCGCGGACTCTAGCATTGTCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGa  <  1:38930/149‑1 (MQ=255)
                                                                                                                                     cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTa                               <  2:336402/115‑1 (MQ=255)
                                                                                                                                     cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTa                               >  1:336402/1‑115 (MQ=255)
                                                                                                                                                  |                                                                                                                                  
TAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGA  >  NC_000913_3_vch_tpiA/2679379‑2679655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: