Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	264,098	0	G	T	100.0%	90.2 / NA	34	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/17);  total (17/17)

GGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAAT  >  NC_000913_3_vch_tpiA/263954‑264241                                                                                                                                                 |                                                                                                                                                ggCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGa                                                                                                                                             <  1:457578/149‑1 (MQ=255) ggCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCAAAAAAGATCACAACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATGGGGCCCATTGCGGTCCTGGCGGGGTTCGGCGTATACCGCTAGGa                                                                                                                                             >  1:47042/1‑149 (MQ=38)         ggCGCAATGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGTAGGTATTGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCg                                                                                                                                     <  1:433721/149‑1 (MQ=255)                             tGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCTATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAATTCGGCGATATCCGGAACGTCTCTGcc                                                                                                                 >  2:50537/1‑149 (MQ=38)                                agtccccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATTAGGCAGGTATAGGGCCCATTGCGTTTCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                                                                                                              <  1:260729/146‑1 (MQ=255)                                aGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                                                                                                              >  1:457983/1‑149 (MQ=255)                                    cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                                                                                                          >  2:350935/1‑149 (MQ=255)                                    cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGAAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGGACGtccctccccgggcaa                                                                                                          >  2:34605/1‑142 (MQ=37)                                     ccgggaaGGGATGCTCATTACAGGTCTCCACTGTTGTCAGTTCAGGGTGATGCTAATTAGGCAGGTAGAGGGCCCATTGCGGTCTGGGCGGCGTTCGGCGTATACCGGTAGGATTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAAt                                                                                                         <  2:149545/143‑1 (MQ=255)                                           agtgTTTCTCATTACATTTCTCCACTATTGTCAGTTCATGGTGATGCTCATCAGGCAGGTATAGTGCCCATTGCGGTCCTTGCGGCTTTCGGTGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGa                                                                                                   <  1:50537/146‑1 (MQ=25)                                                  cTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAcc                                                                                            >  1:369142/1‑149 (MQ=255)                                                   tCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACACCGGCGTTATCCGGAACGTCCCTGCCGGTGTAATGACGGACGCTACCg                                                                                           >  1:380597/1‑149 (MQ=255)                                                      ttACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGGCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACGGACGCTACCGTGc                                                                                        >  1:49108/1‑149 (MQ=255)                                                          aGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACt                                                                                    >  2:281882/1‑149 (MQ=255)                                                          aGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTAGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACt                                                                                    >  1:239039/1‑149 (MQ=255)                                                           gtgccccACTATTGTCAGTCTAGGGTGATGCTCATCAGGTAGGGATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg                                                                                   <  2:218183/144‑1 (MQ=21)                                                               tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAt                                                                               >  2:318105/1‑149 (MQ=255)                                                                 cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAttt                                                                             >  2:470988/1‑149 (MQ=255)                                                                                        gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTACCACTGGTATTTGCCGGTGCAATGGCGAAAGAtgc                                                      >  2:158483/1‑147 (MQ=255)                                                                                         cTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCg                                                     <  1:470988/149‑1 (MQ=255)                                                                                          tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCTGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                                    <  1:2114/149‑1 (MQ=255)                                                                                          tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                                    <  2:369142/149‑1 (MQ=255)                                                                                                 gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGTTACCGTGCCATTGGTATTGGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                                             <  1:20361/149‑1 (MQ=255)                                                                                                 gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                                             <  2:376720/149‑1 (MQ=255)                                                                                                     gTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATg                                         >  2:373899/1‑149 (MQ=255)                                                                                                     gTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATg                                         <  1:281882/149‑1 (MQ=255)                                                                                                                  tgaggTCCTGGGGGCGTTCGGCGTATACCGCTACGCCTGCGGCGATATCCGGAACGTCCCTGACGGTGTAATGACAAGCGCTACCGTGCAACTGGTATTTGCCGGTGCAGTAGCGAAAGACTCGGGACTCAGGATGCTGGCGGTATATc                            <  1:75657/146‑1 (MQ=17)                                                                                                                             cggcgTTCGGCGTGTACCGCTAGGCCTCCGGCGCTATCCGGAACGTCCCTGCCGGTGTAATGACCCACATTATCTTGCTACTTGGATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCt                 <  2:474998/149‑1 (MQ=14)                                                                                                                             cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATCTCGTCATTGCCCt                 <  2:409428/149‑1 (MQ=255)                                                                                                                             cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGATACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCt                 >  2:453172/1‑149 (MQ=255)                                                                                                                                  ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt            <  1:453172/149‑1 (MQ=255)                                                                                                                                     ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAAAGGCTTGACTCAGCATGTTCGCGGTATATCGTCATTGCCCTGCGTTTAc         >  1:488270/1‑149 (MQ=17)                                                                                                                                        ttataCCGCTAGTGCTGCGGTGAGAGGCGGAACGTCCCTGCCGGGGTAATGATACACGCTGCCGTGCCACTGGTATTTGCCGGTGCAGTGGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGa      <  2:416612/148‑1 (MQ=14)                                                                                                                                            aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTACCATGCGCTTATTGATAAt  >  1:400188/1‑149 (MQ=14)                                                                                                                                                 |                                                                                                                                                GGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAAT  >  NC_000913_3_vch_tpiA/263954‑264241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑