Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	2,679,525	0	G	T	100.0%	59.6 / NA	21	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/15);  total (6/15)

GGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACA  >  NC_000913_3_vch_tpiA/2679382‑2679657                                                                                                                                                |                                                                                                                                     ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGCTGTGCGTGAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGa                                                                                                                                 <  1:386491/149‑1 (MQ=255) ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGa                                                                                                                                 <  1:216000/149‑1 (MQ=255)  gTTTTTATTAACAACGGCTTATTCTAATTATATTGTGATGAGCGGCAGCGCGTGCCGCACAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAt                                                                                                                                <  2:385840/149‑1 (MQ=255)       tattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGCTTAGTGAACGCGGTTAAAAATAAAAGGTATTACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATc                                                                                                                           <  1:23410/149‑1 (MQ=255)                        tCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGcgcg                                                                                                          <  1:378001/149‑1 (MQ=255)                                          aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGTTAATCCGGATTAATTGCCGTCAATTCAGCGCGAGCAGACTCAGATTgcgc                                                                                        <  1:53852/149‑1 (MQ=255)                                            cGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTTGTGAATAATGCCGCTTTCGGCCAATCCGGATTTTTAAATCCCGAATCAGCGCGAGCAGACTCATATTGCGCaa                                                                                      <  1:577689/149‑1 (MQ=255)                                              gCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAcc                                                                                    <  1:402045/149‑1 (MQ=255)                                                     gTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGa                                                                             <  1:227706/149‑1 (MQ=255)                                                               aaaatTACAGTTAATAGTGTTTAGTTAACGCGGTTAAAAATAAAAGGCATCACGGTTGTGAATAATGTCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGa                                                                   <  2:472745/146‑1 (MQ=255)                                                                              aGGGTTTAGTTAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTTATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGccac                                                    <  1:173492/149‑1 (MQ=255)                                                                                ggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGTGCAGTCTCATATTGCGCAATCTCCCCGAGTCCCGCGGACGCCAGCATCGccacca                                                  <  1:302073/149‑1 (MQ=255)                                                                                                               aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAATAATCGCCGTAAATTCAACGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTTCTGTGAACGCCATCTTCGGCACCCTCAGGCATTTGTCTTTATGCTTCTGTTGTaa                   >  1:474594/1‑149 (MQ=255)                                                                                                                tCAGGGTTGGGAAGAATGCGTCTTGCGGCCAATGCGGATTAAGGGCCGTTATTTCAGCGCGGGGAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTaaa                  <  2:143094/149‑1 (MQ=255)                                                                                                                tCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATTCTGCCGTTGTaac                  >  2:280884/1‑148 (MQ=255)                                                                                                                tCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTaaa                  <  1:397214/149‑1 (MQ=255)                                                                                                                     gtggtgAATAATGCCGCTTTCTTCCAATCCGTAATAATCGCCGCCAATTCATCGCTAGCAGACTCATATTGCGCAACCTCCCCGAGTTCCTCGTACGCCAGCATCGCCACCATCAGGCATTTTTCTTTTTGCTGCTGTTTTAAATCGcc             <  2:447562/149‑1 (MQ=255)                                                                                                                      tggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCa            >  1:309860/1‑149 (MQ=255)                                                                                                                        gtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCCCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCCTCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCAATa          >  2:497225/1‑149 (MQ=255)                                                                                                                          gAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACa        >  1:331830/1‑149 (MQ=255)                                                                                                                                tGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGGGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCCCCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGaca  >  2:150378/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                     GGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACA  >  NC_000913_3_vch_tpiA/2679382‑2679657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑