Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA2,817,1700CA100.0% 60.7 / NA 24E112* (GAA→TAA) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/12);  total (12/12)

TTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGG  >  NC_000913_3_vch_tpiA/2817030‑2817313
                                                                                                                                            |                                                                                                                                               
ttGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTAGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCg                                                                                                                                         >  1:443402/1‑149 (MQ=255)
      gCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCTTTCCTa                                                                                                                                   >  2:187022/1‑149 (MQ=255)
               cTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTg                                                                                                                          <  2:259425/149‑1 (MQ=255)
                     gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAACGACGACGGCGTCAAAATAATAGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac                                                                                                                    >  1:223824/1‑149 (MQ=255)
                        caacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGACAGCGACGACAGCAACAAAATAATGACGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCAGATCCTACAGCCATTGGGCgacgac                                                                                                                 >  1:57500/1‑149 (MQ=255)
                           caaAACTGGGTCTGTGGCGGGTTTATTGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCTCCAGCAATGCCTCAGCGATGTCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                              <  1:557782/149‑1 (MQ=255)
                             aaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGc                                                                                                            >  2:20975/1‑149 (MQ=255)
                             aaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCATTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGc                                                                                                            >  2:21628/1‑149 (MQ=255)
                                     tccggCGCGGGTTTATTGTGTTTGACGTAATCGGCAACGACGACCGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCTTCTGCGATGGCGCTTTAATTCCTCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtttt                                                                                                    <  1:187022/146‑1 (MQ=255)
                                                 ttATGGTGTTTGACGTGATCGGCAGCGACGATGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTATCTCCTTGTTCCTACATCCATTGGGCGACGACCATGCCAACTTTTCACCACATcaac                                                                                        <  1:336847/149‑1 (MQ=255)
                                                  tATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGTTGCGCCAGCAATGCCTCAGCGATGGCTCTTTAACTCCCCTTTTCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATcaaca                                                                                       <  1:447269/149‑1 (MQ=255)
                                                    tGGTGTTTGACGTGATCGACAGCAACGACGGCGTCAAAATAATGGCGTAATCCCAAGTGCGCCAGCAATGCCACAGCGCTGGCGCTTTAACTCCCCCTTCCTACAGCCATTAGGCGACGACCATACCAACTTTTCACCACATCAACAag                                                                                     >  1:534694/1‑149 (MQ=255)
                                                         gttGAGGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGaa                                                                                <  2:244682/148‑1 (MQ=255)
                                                               gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCTTTCCTACAGCTATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                                                                          <  1:197875/149‑1 (MQ=255)
                                                                        aGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTGTCACCACATCAACAAGAGGAAGCGGTCCGACGCTAt                                                                 >  2:493095/1‑149 (MQ=255)
                                                                           ggcgacgGCGTCAAAAGAATGGGGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTATCTCCCCCTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCca                                                              <  2:129717/147‑1 (MQ=255)
                                                                                                      tCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActt                                                   <  1:107790/133‑1 (MQ=255)
                                                                                                      tCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActt                                                   >  2:107790/1‑133 (MQ=255)
                                                                                                            gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCg                                                                             >  1:286116/1‑101 (MQ=255)
                                                                                                            gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCg                                                                             <  2:286116/101‑1 (MQ=255)
                                                                                                              gcgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                           <  1:85202/149‑1 (MQ=255)
                                                                                                                            tCAGCGATGGCGCTTTAACTCACCGTTCCTGCAGCCATTGGGCTACGACCATGCCAACTTTTCACCCAATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACCAGCCAGCGCATGCgg             >  2:98162/1‑149 (MQ=255)
                                                                                                                                cGATGGCTCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCg         <  2:215063/149‑1 (MQ=255)
                                                                                                                                       gcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTATTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGGTTTTTCACGCGCTAACGCATGCGGGTCGAGATCgg  >  1:187842/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                               
TTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGG  >  NC_000913_3_vch_tpiA/2817030‑2817313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: