| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 1,544,022 | C→A | L13F (TTG→TTT) | narU ← | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 1,544,022 | 0 | C | A | 96.2% | 70.9 / NA | 26 | L13F (TTG→TTT) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (13/12); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAA > NC_000913_3_vch_tpiA/1543897‑1544157 | gcgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCATAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTtct < 1:73178/149‑1 (MQ=255) gcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCAAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCATTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCa > 2:249514/1‑149 (MQ=255) tAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATAGCCAGACATTACTCCGAGCAATATGTGTTCCTTTATTTTCCCCGAAGGCCGGATTTTCGCGTTTCCAGNCGCG‑A‑A‑ATAANANANAGACTATTTTTCTCATATTGc > 1:485284/1‑149 (MQ=255) caGGCAANAAGTAGACAGCTGCTTAATGTCCAGAGATTTCTTCGATCAATATGTATACCTTTATTTTCCCTGTAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAA‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTcc < 2:485284/148‑1 (MQ=255) aGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCt > 1:83605/1‑149 (MQ=255) agaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt < 2:83605/149‑1 (MQ=255) cAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCA‑TTTGCAGTGCCATATTGTTc > 1:431296/1‑132 (MQ=255) cAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCA‑TTTGCAGTGCCATATTGTTc < 2:431296/132‑1 (MQ=255) gactgaTATCCAGAGATTTGTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTaa < 1:426770/149‑1 (MQ=255) gaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTGCCTCACATGCACACATTGGTAATGaa < 1:7744/149‑1 (MQ=255) agaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTACCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACa > 1:296628/1‑149 (MQ=255) cttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac < 1:490457/149‑1 (MQ=255) ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg > 2:457629/1‑149 (MQ=255) tctaGCAATATGTTTTGCTTTGTTTTCCCATAATGCCGGATTTTCTGGTTTCCAGTCGCG‑AAA‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTTCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa < 2:146451/146‑1 (MQ=255) tatGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGGGGTAAGgc > 1:51214/1‑149 (MQ=255) cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgcgccacaacc > 2:551773/1‑142 (MQ=255) tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa > 1:326806/1‑149 (MQ=255) tttATTTTCCCAGAAGGCCGGATTTTCTGGATTCCAGACGCGAC‑A‑C‑AAGATAACAACTCTTTCACTCCTCTTGCAGCGCCAAATTCTTCCTCACATAAAAACCTAGGTCATGAACAAAAGACAACACCCGACGTATGGCGGAATCGccc > 2:13806/1‑148 (MQ=255) ttaagtttCACAGAAGGGCGGATTTTTTGGTTTTCAGGCGCG‑A‑A‑ATAAGATAACGAGTATTTTTCTAATTTTGCACCGCCATATTGTTCCTGACATGTACATATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa < 2:28876/144‑1 (MQ=255) cAGACGGCAGGATGTTCTGTTTTTCAGCTACGAA‑A‑A‑AAGATAACGATGATGTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACCCGCGGTAAGGCGCAATAGCCAGTTATTAGaa < 2:218353/149‑1 (MQ=255) cAGAATGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa < 2:390182/149‑1 (MQ=255) aGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAAt > 1:555775/1‑149 (MQ=255) cGGATTTTCTGGTTTCCAGTCGCG‑AAA‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTACCCTAATGTTCCTCACATGCACACATTGGTAATGAAAAAAAGATAAAACACGGGGTAAGGGGCAATAGCCAGTTATTAGAATTAAGGAt > 1:179445/1‑149 (MQ=255) cGGATTTTCTGGTTTCCAGTCGCG‑AAA‑A‑AAGATAAAGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGAAATAGCCAGTTATTAGAATTAAGGAt > 2:560872/1‑149 (MQ=255) gatgtggtGGTTTCCGGCCGCG‑A‑ATA‑AAGATAACGACTTTTTTTCGTGTTGTGCATTGCGATATTGTTCCGCAAAGACACACATTTTTAATGAAAAAAAGACAAAACACGAGTTAAGGCTCAATAGCCAGTTATTAGAATTAAGGACGa < 2:212765/142‑1 (MQ=255) tttCCAGTCGCGAA‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCATCACCTACACTCATTGGTAATGAAAAACAGACAAAACACGAGATAAGGCGCAATAGACAGTTATTAAAATAAAGAATCAATTGGATGaa > 2:572656/1‑149 (MQ=255) | GCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑A‑A‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAA > NC_000913_3_vch_tpiA/1543897‑1544157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |