| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 1,544,022 | 1 bp→AA | coding (39/1389 nt) | narU ← | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 1,544,021 | 1 | . | A | 77.8% | 93.3 / 22.6 | 36 | coding (40/1389 nt) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base . (6/2); new base A (11/17); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913_3_vch_tpiA | 1,544,022 | 0 | C | A | 100.0% | 115.4 / NA | 36 | L13F (TTG→TTT) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/19); total (17/19) | |||||||||||
GAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑G‑‑C‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA > NC_000913_3_vch_tpiA/1543881‑1544170 ||| gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑G‑‑A‑a‑aaaaga > 1:771548/1‑146 (MQ=255) tAAATAGCATCCAGAAACAGAAGGCAAGAAGTAGACAACTGATTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAATCGC‑AA‑A‑A‑AAA‑GATAACGACTATTTTTCTCAtt < 2:492801/149‑1 (MQ=255) tAGCATCCAGACAGAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑G‑‑A‑A‑AAACGATAACGACTATTTTTTTAAtttttc > 2:485778/1‑147 (MQ=255) tAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGc > 2:668060/1‑149 (MQ=255) aGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCa < 1:481702/149‑1 (MQ=255) aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATCTCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GAAA‑A‑AAA‑CATAAGGACAATTTTTCTCATTTTGCAgg > 1:255447/1‑148 (MQ=255) ccAGACACAGGAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTACGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGcc < 1:224174/149‑1 (MQ=255) acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGGGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTg > 1:333702/1‑149 (MQ=255) gAAGGCATGAAGTAGACAATTGACTGATATCCAAAGATTGCTTCGAGCAATATGTTTTCCTTTATTTTACCAGATGGCCGGATTTTTTGGTTAAAAAAAAA‑AA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc < 2:576923/149‑1 (MQ=255) agaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATGGTTCCTCACAt > 1:768501/1‑149 (MQ=255) gTACACAACTGACTGATAACCAGAGATTTATTCGAGCAATATGTTTTCCTTTATTGACCCAGAAGGCCGGATTTTCTGGTTTAAAAAAAA‑AA‑A‑A‑AAA‑GATAACGACTATTATTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcac < 2:739685/149‑1 (MQ=255) aCAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCTC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAt < 1:732953/149‑1 (MQ=255) aCAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCTA‑‑A‑A‑AAA‑GATAAGGATTATTTTTCTCATTTTATAGTGCCATATTATTCTTCACATGCAAACAt > 2:149966/1‑149 (MQ=255) actgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCCCATGCACACATTgtaggt > 2:594644/1‑144 (MQ=255) tgaTATCCAGAGATTTCTTCGAGCAATATGTGTTCCTTTATTTTCCCAAAAGGCCGCAGTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTCACCGTGCCGTATCGTCAGTCACATGCACACATGTGTAAtaa > 2:586303/1‑147 (MQ=255) tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaa > 2:623464/1‑149 (MQ=255) cAGAGATATCTTCGATCAATAAGTTTTCCTTTATTATCCCACAAGGCCGGATTTTCTTGTTTACAGACGA‑A‑‑AAA‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg < 2:596147/149‑1 (MQ=255) tttCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaaaa > 1:488301/1‑149 (MQ=255) cttcGAGCATTATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac < 1:217907/149‑1 (MQ=255) cGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAg < 1:668060/149‑1 (MQ=255) gAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAgg < 1:463328/149‑1 (MQ=255) gAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTACTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAgg < 1:62639/149‑1 (MQ=255) aaTATGTTTTCCTAAAAATAACAAGAAGGCCGAATTTTCTGGTTTCGAATCGA‑G‑‑A‑ACAAA‑GATAACGAATATATTAAACATCTTCAAGAATTATATTGTTCCTCTCATGCATAGATAAAAAAAAAAAAAAAGACAAAACACGAGGTAAg < 2:798592/149‑1 (MQ=255) tGTTCTCCTTTTTTTTCCCAGTAGGCTGGATTTTCTTGTTTCCAGTGGC‑GA‑A‑A‑AAA‑GATGACGCCTCTTTTGTTCATTTTGCAGTGCCATCTTGTTCCTCTCATTAACACATTGGTAATGAAAAAAAGACAGAACACGAGGTAAGgcgc < 2:609828/149‑1 (MQ=255) ttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAAGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATa > 1:787929/1‑149 (MQ=255) ttCCTTTATTTTCCAAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACTACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATa < 2:538651/149‑1 (MQ=255) ccgttATTTTCCCAGAATCTCGGATTTTCTTGTTTCCAGGCGC‑GA‑A‑A‑AAA‑GATAATGACTATTTTTCTCATTTTTCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAATGACAAAACACGAGGTAAGGCGCAATAGc < 2:815067/146‑1 (MQ=255) cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGcc > 1:437616/1‑149 (MQ=255) ttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg < 1:391882/149‑1 (MQ=255) ttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg < 1:268863/149‑1 (MQ=255) aGGCCGGATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATGTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa < 2:571048/149‑1 (MQ=255) ggATTTTCTGGTTTCCAGTCGC‑GA‑A‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATg > 1:757763/1‑149 (MQ=255) tttCTGGTTTCCAGTCGC‑G‑‑AAA‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAAtt > 2:25834/1‑149 (MQ=255) tCTGGTTTCCGGTCGA‑GA‑A‑A‑AAA‑TATAACGACTAGTGTCACAATTTTTCGGTGCCATATTTTTCCTAGCATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGTAATAGCCAGTTATTAGAATTAAGGATGAATTgg < 2:392358/149‑1 (MQ=255) aGTCGC‑G‑‑AAA‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGGGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAGTTGGGTGAGGTGCt > 2:484057/1‑149 (MQ=255) aGTCGC‑G‑‑AAA‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCt > 1:420052/1‑149 (MQ=255) tcgcgaaa‑aaa‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGa > 1:572324/8‑149 (MQ=255) aa‑aaa‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa < 1:189859/148‑1 (MQ=255) aa‑aaa‑GATAACGACGATTTTTGTCATTTTGCAGTGCCATATTGTTCGTCAGAGGCACACAGTGGTAATGAAAAAAAGACAAAACACGGGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa < 2:477008/148‑1 (MQ=255) ||| GAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑G‑‑C‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA > NC_000913_3_vch_tpiA/1543881‑1544170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |