Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA264,0980GT96.2% 146.7 / NA 52L50L (CTC→CTAykfHuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (29/21);  minor base C (1/1);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATT  >  NC_000913_3_vch_tpiA/263960‑264242
                                                                                                                                          |                                                                                                                                                
cAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                                                                                                                        >  2:866707/1‑149 (MQ=255)
 aGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc                                                                                                                                       >  1:676368/1‑149 (MQ=255)
     gcAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATGGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGTCAGGGCTCCGGCGata                                                                                                                                   >  2:590429/1‑149 (MQ=255)
         tgtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGGATACCGCTAGGACTCCGGCGATATCCg                                                                                                                               >  1:605731/1‑149 (MQ=255)
            aaTCCCCCGACGCAGCCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGaa                                                                                                                            <  1:661253/149‑1 (MQ=38)
             aTCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAAc                                                                                                                           >  1:16132/1‑149 (MQ=255)
             aTCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAAc                                                                                                                           >  1:643088/1‑149 (MQ=255)
                   cGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                                                                                                     >  2:771310/1‑149 (MQ=255)
                        cAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCgg                                                                                                                <  1:261104/149‑1 (MQ=255)
                                tGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGa                                                                                                        >  1:646464/1‑149 (MQ=255)
                                 ggTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAc                                                                                                       >  2:93762/1‑149 (MQ=255)
                                  gcaGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCCGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACa                                                                                                      <  1:748471/147‑1 (MQ=255)
                                          gCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGTGCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCTCTAGGACTCCGGCGATATCCGGAGCGTCCCTGCAGGTGCAAGGGCAGACGCtcc                                                                                              >  1:215413/1‑147 (MQ=25)
                                               ttACAGTTCTCCTATATTGTCAGTTTATGGTGATGCGTATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGc                                                                                         <  1:486473/149‑1 (MQ=21)
                                                 aCAGTTCTCCACTATTGTCAGTTCAGGGTGATGCACATCAGGCAGGTATAGGGCCTATTGCGGCCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCa                                                                                       <  1:929853/149‑1 (MQ=255)
                                                 aCAGTTCTCCACTAGTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCa                                                                                       <  1:71307/149‑1 (MQ=255)
                                                    gTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg                                                                                    <  1:771310/149‑1 (MQ=255)
                                                     ttCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                                         >  2:896188/1‑95 (MQ=255)
                                                     ttCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                                         <  1:896188/95‑1 (MQ=255)
                                                          cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATTACAGACGCTACCGTGCCACTGGTAttt                                                                              <  2:646464/149‑1 (MQ=255)
                                                            cgaTTGTAAGTTCTGGTGGATGCTAATCAGGCAACTATGGCACAGAGTGCGGTCTGGGCGGTGTGCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                            <  2:893654/147‑1 (MQ=17)
                                                            cTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                            >  2:1037451/1‑149 (MQ=255)
                                                                  tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCTTGCCACTGGTAGTTGCCGGTGc                                                                      >  2:444163/1‑149 (MQ=255)
                                                                  tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTCCCGGTGc                                                                      >  1:656570/1‑149 (MQ=255)
                                                                  tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAAACGCTACCGTGCCACTGGTATTTGCCGGTGc                                                                      >  1:1000358/1‑149 (MQ=255)
                                                                      ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                                  >  1:423186/1‑149 (MQ=255)
                                                                        cAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGc                                                                <  1:93762/149‑1 (MQ=255)
                                                                           ggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGaa                                                             >  1:349977/1‑149 (MQ=255)
                                                                                 gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg                                                                                   <  1:452820/121‑1 (MQ=255)
                                                                                 gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg                                                                                   >  2:452820/1‑121 (MQ=255)
                                                                                 gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAAAGCCGCTACCGTGCCACTGGTATTTGCCGGTGCCGTAAGGaaaacttc                                                       >  1:176759/1‑144 (MQ=255)
                                                                                   tcatcaTGCAGGTATAGGGCCCATTACGGTCCTGGCGGCGTTCGGCGTACACCGCTAGGACTCCTGCGATATCCGGAACGTAACTGCCGGTGTAATGACAGATGCTACCGTGCCACTGGTATTCGCCGGTGCAGTAGCGAAAGATTCgg                                                     <  1:944317/149‑1 (MQ=18)
                                                                                          gcaggTATGGGGCCCATTGCGGTGGTGGCAGCGTTCGGCGTATACCGCCAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                                              <  2:155309/149‑1 (MQ=18)
                                                                                              gTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTTCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCCGTAGCGCAAGATTCGGGATTCAGGATg                                          >  2:755044/1‑149 (MQ=18)
                                                                                                 taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTc                                                       >  1:343840/1‑133 (MQ=255)
                                                                                                 taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTc                                                       <  2:343840/133‑1 (MQ=255)
                                                                                                   gggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGc                                     >  1:861020/1‑149 (MQ=255)
                                                                                                   gggCCCATTGCGGTCCTGGCGGCGCTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGc                                     >  2:891595/1‑149 (MQ=255)
                                                                                                    ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGCCTCAGGATGCTGGCg                                    >  1:696141/1‑149 (MQ=255)
                                                                                                       ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTATGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGta                                 <  1:590429/149‑1 (MQ=255)
                                                                                                       ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGta                                 <  2:861020/149‑1 (MQ=255)
                                                                                                        ctaggCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtat                                <  1:60730/145‑1 (MQ=255)
                                                                                                        cactGCGGGCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtat                                >  2:130774/4‑149 (MQ=255)
                                                                                                         aTTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTGCCTGCCGGTGTAATGACAGTCGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtata                               >  2:792591/1‑149 (MQ=255)
                                                                                                           tGCGGTCCTGGCGGCGTTGGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATc                             <  1:628759/149‑1 (MQ=255)
                                                                                                           tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATc                             <  2:676368/149‑1 (MQ=255)
                                                                                                               gTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                          <  1:735293/100‑1 (MQ=255)
                                                                                                               gTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                          >  2:735293/1‑100 (MQ=255)
                                                                                                                tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCAGGAGATATGTGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGGCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATAGCGTcgt                        >  1:516735/1‑147 (MQ=17)
                                                                                                                     gcggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGccc                   <  1:124368/149‑1 (MQ=255)
                                                                                                                             cGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTTGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtttc           >  2:21286/1‑148 (MQ=255)
                                                                                                                                      ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTGCCGTGCCACTGGTATTTGCCGGTGCAGTCGCGAAAGATTCGGGACTCAGGATGCCGGCGGTAGATCGTCATGGCGCTGCGTTTGCTGATAAtt  >  2:17270/1‑149 (MQ=14)
                                                                                                                                          |                                                                                                                                                
CAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATT  >  NC_000913_3_vch_tpiA/263960‑264242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: