Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA264,0980GT100.0% 98.1 / NA 33L50L (CTC→CTAykfHuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/14);  total (19/14)

CCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT  >  NC_000913_3_vch_tpiA/263958‑264243
                                                                                                                                            |                                                                                                                                                 
cccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGCTGCGCATGACAGTTCTCCACTATTGTCAGTTCAGGGTGCTGCTCATCAGGCAGGTATAGGGCCCATTGCAGTCCTGGCGGCGTTCGGCGTATACAGCTAGGACTcc                                                                                                                                           >  2:450604/1‑149 (MQ=255)
 ccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                                          <  1:145880/149‑1 (MQ=255)
           tgtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGCTCAGGGTGATGCTCATCAGGCAGGTATAGGGCGCATTGCGGTCCTGGCGGCGTTCGGCCGATACCGCTAGGAGTCCGGCGATTTCCg                                                                                                                                >  2:413744/1‑149 (MQ=255)
                  tcCCGCTGCAGGCCCCTGGAAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATGAGGCTGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACATc                                                                                                                         >  2:175133/1‑149 (MQ=255)
                   cccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGGTGCTCATCAGGCAGGGATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTcc                                                                                                                        >  2:327835/1‑149 (MQ=255)
                      gCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                                                                                                     <  2:294709/149‑1 (MQ=255)
                         gCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCGCCACTATTGTCAGTTCAGGGTGATGCTCATGAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCg                                                                                                                  <  2:127695/149‑1 (MQ=255)
                             gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                                                                                                              >  1:195573/1‑149 (MQ=255)
                                ccTGGTAGGGATGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATGGGGGCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATGCCCGGTACGTCCCTGCCGGtatact                                                                                                           >  2:38566/1‑147 (MQ=37)
                                       gggTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                                                                                                    <  1:605326/149‑1 (MQ=255)
                                            gCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAc                                                                                               <  2:195573/149‑1 (MQ=255)
                                                            cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAttt                                                                               <  2:390959/149‑1 (MQ=255)
                                                             aCTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTg                                                                              >  2:631955/1‑149 (MQ=255)
                                                              cTATTGTGAGTTCAGGGCGATGCTCATCAGGCAGTTATAGGGCCCATGGCGGTCCGGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                             <  2:624103/149‑1 (MQ=21)
                                                                       gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATcc                                                                                                                                 <  1:75262/88‑1 (MQ=255)
                                                                       gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATcc                                                                                                                                 >  2:75262/1‑88 (MQ=255)
                                                                       gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGt                                                                    >  2:345839/1‑149 (MQ=255)
                                                                        ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAtt                                                                                >  2:62437/1‑136 (MQ=255)
                                                                        ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAtt                                                                                <  1:62437/136‑1 (MQ=255)
                                                                          cAGGGTGAGGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGc                                                                 <  2:566671/149‑1 (MQ=255)
                                                                                            gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCCATATCCGGAACGTCCCTGCCGGTGTACTGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGGAAGATTCGGGACTTAg                                               >  2:434038/1‑149 (MQ=18)
                                                                                                  ataGGGCCCATTGCGGTCCTGGCCGCGTGCGGGCTATACCGCTAGGCCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGGAGTAGCGAAAGATTCGGGACTCAGGATGCt                                         <  2:478627/149‑1 (MQ=18)
                                                                                                   taGGGCCCATTGCGGTCCTGTCGGCGTTCGGCGTATACCGCTGGGTGTGCGGCGATATCCGGAACGTGCCTGCCGGTCTACCGTCTGGCACTCCGGTTCCGCTGGTATTTTCCTGTGCCGTCCCGAATGATTCGAGCCCCCGGctgctg                                        >  2:243894/1‑149 (MQ=255)
                                                                                                      ggCCCATTGCGGTCCTGGCGGCGTGTGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCg                                     <  2:395094/149‑1 (MQ=255)
                                                                                                                ggTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTc                           >  1:569515/1‑149 (MQ=255)
                                                                                                                             ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt              >  1:603934/1‑149 (MQ=255)
                                                                                                                             ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt              <  1:631955/149‑1 (MQ=255)
                                                                                                                             ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAACGAAAGATTCGGGACTCAGGATGCTGCCGGTATATCGTCATTGCCCTGCGtt              >  2:202972/1‑149 (MQ=255)
                                                                                                                                ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGGCGGTGTAATGACAGACGCTACCGTGCCCCTGGGCTTTGCCGGTGCAGGAGCGAAAGAAATGGGGCTCAGGATGCCCGCGGTAAATCGGAATTTCACTGCGATTAc           >  2:531631/1‑149 (MQ=17)
                                                                                                                                 gCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACt          >  1:170097/1‑149 (MQ=255)
                                                                                                                                       aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGc                      <  2:587586/131‑1 (MQ=255)
                                                                                                                                       aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGc                      >  1:587586/1‑131 (MQ=255)
                                                                                                                                       aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGGGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGGGGTACATCGTCATTGCCCTACGTTTCCTGATAAt    >  2:971/1‑149 (MQ=14)
                                                                                                                                         cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAAttt  >  2:90762/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
CCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT  >  NC_000913_3_vch_tpiA/263958‑264243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: