Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.45.6% inf / inf 999intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (306/221);  new base . (321/120);  total (634/365)
Fisher's exact test for biased strand distribution p-value = 2.13e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.14e-01

Only 100 of 1237 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377
                                                                                                                                    |                                                                                                                                  
  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttt                                                                                                                                  >  1:228357/1‑128 (MQ=255)
  aataTTTAGTTTCATTAAATTATAGCAATTGGATGTAATTGTAATAAAAAAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAAATAATTTTAAATTGTAAAAAATAGC‑‑ttt                                                                                                                                  <  2:1812682/128‑1 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttt                                                                                                                                 >  2:944232/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttt                                                                                                                                 >  2:395949/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttt                                                                                                                                 >  1:2969390/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttttt                                                                                                                              >  1:3815889/1‑128 (MQ=255)
      tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttttttt                                                                                                                             >  2:2970399/1‑128 (MQ=255)
        tattttCATTAAATTATAGCACTTTTTTTAATTAGTAACAAAATAAAAATAATATCTTTTTATATATTTTTTTTATATGCAAATTTTTTAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑ttttttttt                                                                                                                           <  1:3449709/125‑1 (MQ=255)
        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttttttttt                                                                                                                           >  1:1148259/1‑128 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttttttttttt                                                                                                                         >  1:1043048/1‑128 (MQ=255)
           tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttttttttttt                                                                                                                        >  2:1875736/1‑128 (MQ=255)
           tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttttttttttt                                                                                                                        >  1:2354649/1‑128 (MQ=255)
                     ttttCGCACTTTTATTTAATAGTAATTAAATAAAAATATTATCTTTTTATATTTTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAAAAGC‑‑ttttttttttttttttttatat                                                                                                              <  1:3153638/125‑5 (MQ=255)
                     ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTtaaa                                                                                                              >  2:3837962/1‑126 (MQ=255)
                       aTCGCAGTTGTTTGAATTATTAATAAACAAAAACTAATATCTTTTTAGTAATTTTTTGTATATTGAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAAAAGC‑‑TTTTTTTTTTTTTTTTtataatgg                                                                                                            <  1:311809/128‑5 (MQ=255)
                        ttgCACTTGTTTGAATTATTAATAAAATAAAATTATTATCTTTTTTTTTATTTTTTTTATATGCAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATTG‑‑GTTTTTTTTTTTTTTTTTTATAATgg                                                                                                          <  2:514181/126‑1 (MQ=255)
                        tCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTtaataaat                                                                                                           >  1:1591967/1‑126 (MQ=255)
                         cggatttttttGAATTAGTAATAAAATAAAATTTTTATATTTTTATATATTTTTTTTATTTGTAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGa                                                                                                         <  2:2172952/118‑1 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑tttttttttttttttttaaaattaaa                                                                                                          >  1:505778/1‑119 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCATATTAGATATTTATTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAATATATC‑‑ATTTTTTATTTTATTATTTaaaagaa                                                                                                          >  1:765246/1‑125 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAata                                                                                                     >  1:2060753/1‑128 (MQ=255)
                              ttttATTAATTATTTATAAAATAAAAATATTATATTTTTTTATATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAata                                                                                                     <  1:881909/125‑1 (MQ=255)
                               tGTATTAATTAATTATAAACAAAAAATATTATTTTTTTAGTTATTTTTTGTATATGCAAAATTTTAAAAAATTAATTTTAACTTGTAAAAAAAAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                                                 <  1:1294737/128‑1 (MQ=255)
                                 tATTAATTATTTATAAAATAAAACTATTATATTTTTTTATTTTTTTTTTATATTTAAATTTTTATAAAATTTATTTTAAATTTTAAAAAATATC‑‑TTTTTATTTTTTTTTTTTTTATAATGGAAAtata                                                                                                  <  2:2851469/128‑1 (MQ=255)
                                 tATGAATTATTAATAAAATAAAAATATTATTTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  1:1409667/128‑1 (MQ=255)
                                 tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTAGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAATAATTAAAAAATaaa                                                                                                >  1:2486160/1‑128 (MQ=255)
                                  aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATAATtaaaataaaat                                                                                                >  2:2765429/1‑127 (MQ=255)
                                   ttaaTTAGTAATAAAATAAAAATATTATCTTTTTATTTATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  1:3349412/126‑1 (MQ=255)
                                    ttatttttaattaacaaaaaataTTTTTTTTTTATATTTTTTTTTTTTATTCAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  1:3911621/116‑1 (MQ=255)
                                    ttatatttataaaaataaaaataTTATTTTTTTTTTTATTTTTTTTTTATTCAAATTTTTAAAAAAATAATTTTAATCTTTTAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  2:346784/117‑1 (MQ=255)
                                     aattttttaGAAAATAAAAATTTTTTCTTTTTTTATATTTTTTTTATTTTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                                                          <  2:1411919/120‑1 (MQ=255)
                                     aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAAtttt                                                                                            >  1:3529887/1‑128 (MQ=255)
                                       ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAATAATAAAAAAATAAATTTTa                                                                                          >  2:1016795/1‑128 (MQ=255)
                                        ttttaattaaataaaaattTTTTTTTTTTTTATTTTTTTTTTTTATTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATTTT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                                                         <  2:3084553/117‑1 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTTAATATAAATTTTatt                                                                                        >  1:3659624/1‑126 (MQ=255)
                                         aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTTAAAAAAAAAGA‑‑TTTTTTTTTTTTTTTTTT‑‑TAATTGAAATATTAAATTTTAgtt                                                                                       >  2:1284220/1‑126 (MQ=255)
                                            aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTTAAATAAAAATTTTattaatt                                                                                    >  1:1827511/1‑122 (MQ=255)
                                              gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTAAATTTAAAAAAAAAATTTATTTAtttt                                                                                  >  2:3685281/1‑128 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAGTTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAATTATATAtttttttttttttg                                                                                  >  2:1628010/1‑118 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTTTATAATGTAAAATAAAtttttttgttttt                                                                                   >  2:2061177/1‑119 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTAAAATTAAAATTTTattatttttt                                                                                 >  2:1131141/1‑119 (MQ=255)
                                                aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATTAAAAAAAAAATTTAATTTAtttt                                                                                  >  1:1478013/1‑128 (MQ=255)
                                                    aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTTTTttgtttta                                                                            >  1:3237350/1‑125 (MQ=255)
                                                      aataattattTTTTTTTATTTTTTTTTTTTATTTAATTTTTTAAAAAATTATTTTTAATTTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                                                         <  1:1649230/124‑1 (MQ=255)
                                                       actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTTATGGAAATATAAATTTTATGTATTTTTTTGTCTaat                                                                        >  1:3754941/1‑127 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATTTAAATATAATTTTTATTTATTTTTTTTTATaa                                                                         >  1:657927/1‑128 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTATaat                                                                        >  1:3289185/1‑127 (MQ=255)
                                                          tttatTTTTTTTGTTATTTTTTTTTTATGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                                                      <  2:2525662/127‑1 (MQ=255)
                                                           ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTATTTTTTTTTATaatt                                                                       >  1:2795026/1‑126 (MQ=255)
                                                            tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAAATATAAATAAAAATTTAATTTATTTTTTTATATaataaaa                                                                    >  2:2438891/1‑123 (MQ=255)
                                                            tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTTTTGTATAAGtatata                                                                  >  1:827457/1‑128 (MQ=255)
                                                               cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGTTATTTTGTTTTATAAGTATATaaaaa                                                              >  2:146725/1‑128 (MQ=255)
                                                                tttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTTTATAATTATATaaa                                                                >  1:2738657/1‑128 (MQ=255)
                                                                  tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATATaaaaa                                                              >  1:2660234/1‑128 (MQ=255)
                                                                   ttATATTTTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAATAG‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                                                            <  2:1343282/128‑1 (MQ=255)
                                                                   ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTTTTTTTTTTTATAATTATaaaaaaaa                                                             >  2:72715/1‑128 (MQ=255)
                                                                   ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTTGTTTTTTTTTTTTATAATTATGaaaaaaaa                                                            >  2:3889830/1‑128 (MQ=255)
                                                                      taatttttttGTATATTTAAAATTTTAAAAAAATATATTTTAATTTTATAAAAAACTCT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaa                                                             <  1:3062579/124‑1 (MQ=255)
                                                                      gATTTTTTTTGTTTATGTAAATTTTTAAAAAATTTATTTTTACTTGTAAAAAATTG‑‑GTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                                                         <  2:3542044/128‑1 (MQ=255)
                                                                       ttatTTTTTTTATTTTTAAATTTTTAAAAAATTATTTTTAAATTTTTAAAAAT‑‑T‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                                                        <  1:2453844/127‑1 (MQ=255)
                                                                        tatTTTTTTTATTTTAAAATTTTTAAAAATATAATTTTAACTTTTAAAAAAATG‑‑GTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                                                       <  1:2014065/128‑1 (MQ=255)
                                                                         atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTGTATAATTATATAAAAAAATATaaa                                                      >  1:1004419/1‑128 (MQ=255)
                                                                          ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTTTGTATTTTTTTTTATAATTATCTAATAAAAAATAAAg                                                     >  2:211544/1‑128 (MQ=255)
                                                                              tttttttatttaaaTTTTTAAAAATTTAATTTTAAATTTTAAAAAATAT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                                                   <  2:2678483/117‑1 (MQ=255)
                                                                                tttttatttaaaTTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                                                <  2:31377/119‑1 (MQ=255)
                                                                                    atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGAAATTATAAATTTTAGGTTTTTTGTTAAATAAGTATCAAAAAAAATAGAAAGTaaataaat                                            >  1:2126754/1‑126 (MQ=255)
                                                                                    atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTAAATGAAAAATAAATTTTAAGTTATTTTTTTTAAAAATTATATAAAAAAAAAAAATTAAAAAAATAtt                                          >  2:1559681/1‑128 (MQ=255)
                                                                                     tttttaaTTTTTTAAAAATTTATTTTTAATTTTAAAAAATATA‑‑TTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                                             <  1:3730119/123‑1 (MQ=255)
                                                                                      tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTTAATGGAAAATTAAATTTATGTTTTTTTTTTTTAAAATTAAAAAAAAAAATAAAAAGTAAAAAatat                                           >  2:851565/1‑128 (MQ=255)
                                                                                      tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTTTGTTTTTTTTTTTGTAAGTATATAAAAAAATTTAAAGAAAAAAATAtt                                          >  2:1698290/1‑128 (MQ=255)
                                                                                       gCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTGTTTTATAATTATATAAAAAAATAAAAATTaaaaataattt                                         >  1:1506320/1‑122 (MQ=255)
                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTTTTTAAGTATATAAAAAAATATAAAGAAAAAAatatat                                         >  1:3584810/1‑126 (MQ=255)
                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑CTTTTTTTTTTTTTTTTTTTTAATGGTAATATAAATTTTATGTATTTTTTTTTATTATTATATAAAAAAATATAAATTaaaaatttttt                                         >  1:1212057/1‑122 (MQ=255)
                                                                                          aaTTTTTAAATAATTAATTTTTAATTTTAAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa                                      <  2:2279374/128‑1 (MQ=255)
                                                                                            ttttAAAAAATTAATTTTAAATTTTAAAAAATTT‑‑ATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaa                                    <  1:944232/128‑1 (MQ=255)
                                                                                                  tttttaaaaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTTATTGGAAATATAAATTTTAGGTTTTTTTTTGTATAATTAAATAAAAAAATAAAAAGGAAAAAATATTTTTAAAATTa                                >  2:3259922/9‑128 (MQ=255)
                                                                                                    aaattaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTATAATTTTATAAAAAAATATAAAGTAAAAAATATTTTTAAATGTAATAc                            >  1:2603504/2‑128 (MQ=255)
                                                                                                     aattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTAAAATGGAAATATAAATTTTAGGTATTTTTTTTTATAATTATATAAAAAAATATAAATGAAAAATTATTTTTAAAATTAATa                             >  2:3191198/1‑128 (MQ=255)
                                                                                                      attaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATTAATGGAAAAAAAATTTTTTGGTTTTTTTTTGTCTATGTATCAAAAAATATATAAAGTAAAAAATATTTTTTAAAGTATTag                            >  1:1743217/1‑127 (MQ=255)
                                                                                                       ttaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTATAAGTATATAAAAAAATATAAAGTAAAAAATATTTTTAAATTtaataataa                         >  1:2410813/1‑128 (MQ=255)
                                                                                                         taattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGGTTTTTTTTGTATAATTTTAAAAAAAAATAGAAATGAAAAAATTTTTTTaaattttaataa                           >  1:2213852/1‑119 (MQ=255)
                                                                                                         taattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATAAAATTTTTAGTTTTTTTTTTTTATATTTATATAAAAAAATTTAAAGTAAAAAATTTTTTTAAAATTATtaataa                         >  1:578544/1‑128 (MQ=255)
                                                                                                              tGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATGTAAATTAAAATTTTAGTTTTTTTTTTTTAAAATTAAAAAAAAAAAAAAAATGAAAAAAAAATTTTTAAAATTAAAAAAAAAattt                    >  2:64484/1‑127 (MQ=255)
                                                                                                                taattttAAAAAATATT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAattat                   <  1:1356659/122‑1 (MQ=255)
                                                                                                                 aCTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAATTTAAATTTTAGTTTTTTTTTTGTCTAAGTATATAAAAAATTATAAAGTAAAAAATATTTTTAAATTTAATAATAAAAtttttt                 >  2:3025652/1‑128 (MQ=255)
                                                                                                                  tttttAAAAAATTAT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTAttt                 <  2:3345002/124‑1 (MQ=255)
                                                                                                                  cTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTTATTTGAAATAAAAATTTTAGGTTTTTTTTTTTCAATTTATAAAAAAAAAAAAAAAGGAAAAAATATTTTTAAAAATAATAAAAAAATTATTTa                >  2:443098/1‑128 (MQ=255)
                                                                                                                  cTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTATATTTTTATAAAAAAATATAAATGAAAAAATATTTTTAAAATTAATAATAAAATTATTTa                >  1:2553643/1‑128 (MQ=255)
                                                                                                                        ttttaaaatttttttttttttttttttttttATAATTTAAATATAAATTTTAGTTATTTTTTTGTCTAAGTATCTAAAAAAATAGAAAGTAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt              <  1:1922536/116‑1 (MQ=255)
                                                                                                                        tttaaaaatttttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt              <  2:3724745/116‑1 (MQ=255)
                                                                                                                           taataattttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTATAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGtt           <  2:2383212/119‑1 (MQ=255)
                                                                                                                           aaaTAGC‑‑TTTTTTTTTTTTTTTTTTTAAATGGAAATTTAAATTTTAGGTATTTTGTGGTATAATTATCTAAAAAAATAGAAAGGAAAAAATATTTTTAAAATTAATATAAAAATTATATAATGttaat        >  1:3397602/1‑128 (MQ=255)
                                                                                                                            aaattttttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGtta          <  2:1209090/120‑1 (MQ=255)
                                                                                                                            aaaaaatgtttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGtta          <  2:3424589/120‑1 (MQ=255)
                                                                                                                                tgtttttttttttttttttatttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta      <  2:3768508/124‑1 (MQ=255)
                                                                                                                                attttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta      <  1:417996/124‑1 (MQ=255)
                                                                                                                                aatttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta      <  2:3624151/124‑1 (MQ=255)
                                                                                                                                 ttttttttttttttttttttttATAATTGAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaattaa     <  1:288468/125‑1 (MQ=255)
                                                                                                                                 c‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTAGTTTTTTTTTTTTGTAATTATAAAAAAAAATAAAATGTAAAAAATATTTTTAAAATAAATAAAAAAATTATTTAATTTAAATAaaaa   >  2:1963469/1‑128 (MQ=255)
                                                                                                                                    tttttttttttttttttttATAATGTAAAAATAAATTTTTGTTATTTTTTTGTATAAATATCAAAAAAAATATAAAGTAAAAAATATTTTTAAAAATAATAAAAAAATTATTTAATTTTaaaaaaaaa  >  2:1083984/1‑127 (MQ=255)
                                                                                                                                    |                                                                                                                                  
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: