Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.65.5% 89.1 / inf 242intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (20/50);  new base . (51/82);  total (90/152)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 287 total aligned reads displayed.

AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAA‑‑‑AAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAAT  >  CP016816/202358‑202601
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aaGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCt                                                                                                                          <  2:3955952/128‑1 (MQ=255)
 attttttgtAAATAATATATTTAAGTTTAATTAAAATAACATAAACTTAATTATTATTATAATGTAGATACTAGTTATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAATCCCCtt                                                                                                                         <  2:2280503/122‑1 (MQ=255)
  gTTAGTTTAAATAATATAGTTAATGTTAATTAAAATACCATAAACTTAAAAATTCTTATAATTTATATCCTCGTTTTCTATTTTTTTATTTGTTTTTCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                        <  2:3869748/128‑2 (MQ=255)
  gTTAGTGTAAATAATATAGTTAATGTTAATTAAAATACCAAAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTTTTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                        <  2:2985703/128‑2 (MQ=255)
  gTTAGTGTAAATAATAGTGTTAAGGTTAATTAAAATAACATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCttaa                                                                                                                       <  2:1078830/128‑3 (MQ=255)
  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                        <  1:3819055/128‑2 (MQ=255)
  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACCtta                                                                                                                        >  1:3283849/1‑127 (MQ=255)
  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCCtta                                                                                                                        >  1:3431770/1‑127 (MQ=255)
     aGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTATTATTTTTTAATCTTCACAAATAAAAAAAAAACAAAACCCttaaaa                                                                                                                     >  2:2166043/1‑124 (MQ=255)
     aGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCAttaaaa                                                                                                                     >  2:3840125/1‑124 (MQ=255)
       tgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTTGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCCttaaaaat                                                                                                                   >  2:3201110/1‑122 (MQ=255)
        tttaataatATAGTTAAGGTTAATAAAAATACAATAAAATTAATAATTCATAAAATTTAGATCCTCGTGATATATTTTTTTATTTGTTTAACTTCACAAATAAAAAAAAAACAATCCCCttaaaaatt                                                                                                                  <  1:2420134/125‑8 (MQ=255)
           aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTGGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCC‑‑attaa‑‑‑aaattaaaa                                                                                                         >  1:3341020/1‑128 (MQ=255)
           aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaaaattaaa                                                                                                               >  2:2983957/1‑118 (MQ=255)
            ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACCtt‑‑‑a‑‑‑aaaataaaaa                                                                                                        >  2:912992/1‑128 (MQ=255)
             taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAATACAC‑‑ttaa‑‑‑aaattaaaaa                                                                                                        >  1:2606070/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATTTAGATCCACTTGATCTATTTTTTTATTTTTTTATCTTAAAAAATAAAAAAAAAACAAT‑‑ACAATAAA‑‑‑AAAATaaaaaaaa                                                                                                     >  2:1385024/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑A‑‑‑AAAATaaaaaaaaa                                                                                                    >  1:3035482/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaa                                                                                                     >  2:3677425/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATAACATT‑‑‑A‑‑‑AAAATaaaaaaaaaa                                                                                                  >  2:1214077/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTACACAAATAAAAAAAAAACAATACCC‑‑TTAA‑‑‑AAATTaaaaaaaaa                                                                                                    >  2:3632314/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaa                                                                                               >  2:3366490/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaa                                                                                               >  2:3500837/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaa                                                                                               >  2:3702872/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATAACCTCGTTATATATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaa                                                                                              <  1:288926/128‑1 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaaa                                                                                             >  2:3231204/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAA‑‑‑AAATTaaaaaaaaaaaaaaa                                                                                             >  2:1011047/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTATTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAAACAC‑‑‑AAA‑‑‑AAAATtaaaaaaaaaaaaaaa                                                                                            >  2:487486/1‑128 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaaaaa                                                                                           >  2:3538457/1‑128 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAATTGTTTATCTTCACAAATAAAAAAAAAACAATACC‑‑‑ATAA‑‑‑AAAATtaaaaaaaaaaaaaaaaa                                                                                          >  2:2533912/1‑128 (MQ=255)
                          ttttattaaAAAAATATAAACTTATAAATTTTTAAAATTTATAAACTATTTATATATTTTTTTATTTTTTATTATAAAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaaaaaa                                                                                          <  1:2489718/124‑1 (MQ=255)
                          gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATATTCACAAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaaaaaa                                                                                          >  2:3143326/1‑128 (MQ=255)
                          gTTAATTAAAATAAAATAAAATTAATAATTAATAAAATTTATATCATAGTTATCTATTTTTTTATTTTTTTATATTAACAAAAAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaaaaaa                                                                                          <  1:1884577/128‑1 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTATTTAACTTCACAAAAAAAAAAAAAAAAAACC‑‑‑AAAAA‑‑‑AAAATaaaaaaaaaaaaaaaaaaaaa                                                                                       >  2:1511183/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATACTCTTAATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAAAACAC‑‑ATAA‑‑‑AAATTaaaaaaaaaaaaaaaaaaaaa                                                                                       >  1:3532206/1‑128 (MQ=255)
                             aaataaaataaaaTAAACTTTATAATTATTATAATTTATCTACTATGGATATTTTTTTTTATTTTTTTATATTAAAAAATAAAAAAAAAAAAAACCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaaaaaaaaa                                                                                       <  1:3028659/125‑1 (MQ=255)
                              attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAAAAAAAATAA‑‑‑AAATTaaaaaaaaaaaaaaaaaaaaa                                                                                       >  2:204118/1‑128 (MQ=255)
                              attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATATTTTTATTTGATTATCTTCACAAATAAAAAAAAAAAAATCCACAaaaaa‑‑‑aaaaaaaaaaaaaaaaaaaaaaaaa                                                                                        >  2:1861461/1‑128 (MQ=255)
                              attaaAATACCATAAACTTAATAATTATTATAATATATATAATCGTGATCTATTTAATTATTTATTAATCTAAAAAAATAAAAAAAAAAAAT‑‑CAAATTAA‑‑‑AAATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                     >  1:1425680/1‑126 (MQ=255)
                              attaaAATACCATAAACTTAATAATACTTATAATGTAGATCAACTAGATCTAATTTTATATTTATTAAACTTCACAAAAAAAAAAAAAAAAAACCC‑‑‑AAAA‑‑‑AAAATaaaaaaaaaaaaaaaaaaaaaaa                                                                                     >  2:1716103/1‑126 (MQ=255)
                               ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCAATTAAAA‑‑‑ATaaaaaaaaaaaaaaaaaaaaaaaa                                                                                       >  1:3580429/1‑128 (MQ=255)
                                 aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                   >  2:3049022/1‑124 (MQ=255)
                                  aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAACTTCACAAATAAAAAAAAAACAATCCCATT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACAaaaa                                                                                 >  2:3257135/1‑128 (MQ=255)
                                  aaaTACCATAAACTTAATAATTCTTATAATGTAGATACTCTAGATCTATTTTTTTATTTTTTTATATTAAAAAATAAAAAAAAAAAAAT‑‑AAAATAAA‑‑‑AAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                  >  2:2658250/1‑123 (MQ=255)
                                       ccATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTaaaaaaaaaaaaaaaaaaaataaaaaaaaaa                                                                             >  1:3102395/1‑117 (MQ=255)
                                       acaaaaaaTTAATAAATATTATAATTTATATAATTTTTAAATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAaaacaaa                                                                            <  1:1355799/124‑1 (MQ=255)
                                            aaattAATAATTCTTAAAATTAATAAACTCTTTATATTTTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAACAATCCCCTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                       <  1:3265260/125‑1 (MQ=255)
                                              attAAAAATTTTTATAATTTATATACAATTTATTTATTTTTTTATTTTTTTATCTTAAAAAATAAAAAAAAAAAAATCCCCTTAAA‑‑‑AATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                       <  1:2984129/127‑1 (MQ=255)
                                                taaaaaTTTTTATAATTTAGTAACTCGTTATATTTTTTTTTTTTTTTTTATATTCAAAAATAAAAAAAAAAAAAACCCC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAttt                                                                    <  2:3599478/128‑1 (MQ=255)
                                                 aataaataTTATAATGTATAACCTCGTTATATATTTTTTTATTTTTTTATCTTCAAAAAAAAAAAAAAAACAATCCCCTT‑‑‑A‑‑‑AAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaa                                                               <  1:3370337/128‑1 (MQ=255)
                                                  ataatTATTATAATTTATATCCAGGTGATCTATTTTTTTATTTTTTTATCTTCAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCt                                                                  <  1:3642137/128‑1 (MQ=255)
                                                             aaTTTAGATACACGTGTTTTATTTTTTTATTTGTTTATAATAACAAATAAAAAAAAAAAAACCACTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt                                                    <  1:3498849/128‑1 (MQ=255)
                                                                   ttatcacGTGTTTTATTTTTTTTTTTTTTTATCATAAAAAAAAAAAAAAAAAAAATACCCATAAA‑‑‑AATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                  <  2:3613599/122‑1 (MQ=255)
                                                                     ttatttttttaaaTTTTTTTTTTTTTTTATATTAAAATATAAAAAAAAAAAAATTCACTTT‑‑A‑‑‑AAATTTAAAAAAAAAAAAAAAAAAAATAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                              <  2:81037/116‑1 (MQ=255)
                                                                      tatttttatattttttttttttttttTATTTTAAAAAAAAAAAAAAAAAAAAACCCATAAAA‑‑‑AAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                               <  1:2287913/116‑1 (MQ=255)
                                                                      acatttttattATTTTTTTTTTTTTTTATTATAAAAAAAAAAAAAAAAAAAAATCCC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                              <  1:870736/118‑1 (MQ=255)
                                                                      aaaatttatatatttttttattttttTATATTAAAAAAAAAAAAAAAAAAAAAACCC‑‑ATAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                              <  1:103008/118‑1 (MQ=255)
                                                                        attttttatATTTTTTTTTTTTTTTATAATAAAAAAAAAAAAAAAAAAATAAACTTAAAA‑‑‑AATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                             <  1:604065/120‑1 (MQ=255)
                                                                          ttttttttttttttttttttttttTATAAAAAAAAAAAAAAAAAAAAAACCCATTAAAA‑‑‑ATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcaca                                            <  1:496538/120‑1 (MQ=19)
                                                                           tttttttttttttttttttttTATTTTAAAAAAAAAAAAAAAAAAAATACCC‑‑TTAT‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                         <  2:398455/121‑1 (MQ=255)
                                                                            attatatttttttattttttATAATTAAAAAAAAAAAAAAAAAAAAAACCATT‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                       <  1:1188682/124‑1 (MQ=255)
                                                                             tatttattttttattttttaATTTTAAAAAAAAAAAAAAAAAAAATCACC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATATCTTAAAATTCCCTATTATACCACatttta                                       <  2:2752800/122‑1 (MQ=255)
                                                                             taaattttttttATTTTTTAATTTTAAAAAAAAAAAAAAAAAAAAACCC‑‑ATAAA‑‑‑AAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                       <  1:3167109/123‑1 (MQ=255)
                                                                             atttatttttttattttttTATTTTAAAAAATAAAAAAAAAAAAATACCCTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatcttatt                                     <  1:3560551/125‑1 (MQ=255)
                                                                              atattttttttATTTTTAATAATAAAAAAAAAAAAAAAAAAAAACCCC‑‑ATAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatt                                     <  1:1173529/124‑1 (MQ=255)
                                                                               cTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAAAAAAAAATATTTCTTAAAATTCACTATAATTCCACattttatat                                    >  2:2070198/1‑126 (MQ=255)
                                                                                 aattttttttttttttttttATAAAAATAAAAAAAAAAAAAAACCCATT‑‑A‑‑‑AAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                   <  1:3538457/126‑1 (MQ=255)
                                                                                  tttttttatttttttaTATTAAAAAATAAAAAAAAAACAAACCCCTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                 <  1:912992/128‑1 (MQ=255)
                                                                                  tttttttatttttttaTATTAAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                  <  1:272589/128‑1 (MQ=255)
                                                                                  tttttttATTTTTTATTTTAAAAAATAAAAAAAAAAAAAAACCTTTTAA‑‑‑AATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCATATTTTAttttt                                  <  1:2073840/128‑1 (MQ=255)
                                                                                  tttttttATATTTAATCTTAACAAAAAAAAAAAAAAAAATACCCTTAAA‑‑‑AAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                  <  1:1115188/128‑1 (MQ=255)
                                                                                   ttttttttttttttATATTAACAAAAAAAAAAAAAAAAAATCCCTTAAA‑‑‑AAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                  <  1:3297069/128‑1 (MQ=255)
                                                                                   ttttttttttttttATATTAAAAAAAAAAAAAAAAAAAAAACCATTAAAA‑‑‑ATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                   <  2:76658/128‑1 (MQ=255)
                                                                                   ttttttttttttTAAAATTTAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAA‑‑‑AAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                 <  1:1214077/128‑1 (MQ=255)
                                                                                   ttttttatttttttatATTAAAAAATAAAAAAAAAAAAATACCC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                 <  2:3824318/128‑1 (MQ=255)
                                                                                   tttttaATTTTTTTATATAAAAAAAAAAAAAAAAAAAAAT‑‑ACAATAAA‑‑‑AAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                 <  1:445315/128‑1 (MQ=255)
                                                                                     ttttatttttttatttTAAAAAATAAAAAAAAAAACAATCCC‑‑AATAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                <  2:3792918/128‑1 (MQ=255)
                                                                                     ttttATTTTTTTATAATAACAAATAAAAAAAAACAATCCCATT‑‑‑A‑‑‑AAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                           <  1:2509396/128‑1 (MQ=255)
                                                                                     ttttAATTTTTTATATTAACAAATAAAAAAAAAAAAATCCCCAT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                              <  1:3063377/128‑1 (MQ=255)
                                                                                      tttATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCCATAAA‑‑‑AAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                               <  1:746761/128‑1 (MQ=255)
                                                                                       ttATTTTTTTATATTAAAAAAAAAAAAAAAAAAATAAC‑‑ATTAA‑‑‑AAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                            <  1:380043/128‑1 (MQ=255)
                                                                                          ttttttTATATTAAAAAAAAAAAAAAAAAAAAAACAC‑‑TTAA‑‑‑AAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                         <  2:3035482/128‑1 (MQ=255)
                                                                                          tttGTTTAAATTAAAAAAAAAAAAAAAAAAAATACCATTAAAA‑‑‑ATTAAAAAAAAAAAAAAAAAAAAACACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                            <  1:2775978/128‑1 (MQ=255)
                                                                                            tGTTTATATTTAAAAAATAAAAAAAAAAAATTCCCTTT‑‑‑‑‑‑AAAATTTAAAAAAAAAAAAAAAAAAACTAAAAACTAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                       <  2:3322064/128‑1 (MQ=255)
                                                                                              tttATATTAAAAAATAAAAAAAAAAAAATACCCTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                     <  1:1598060/128‑1 (MQ=255)
                                                                                               ttATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAACTATATTTATTAAAATTACAAATTAAAACAAATTATATTTTTAAAATAAAATAtt                     >  1:3200953/1‑128 (MQ=255)
                                                                                                tttattataaaaaaaaaaaaaaaaaaaATACATTTA‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                     <  2:2351129/119‑1 (MQ=255)
                                                                                                tatatttaaaaaaaaaaaaaaaaaaATACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                         <  1:3610189/119‑1 (MQ=255)
                                                                                                tataTTAACAAATAAAAAAAAAAAAATCCCC‑‑TTAA‑‑‑AAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTg                    <  2:3269553/128‑1 (MQ=255)
                                                                                                    ttaaaaaaaaaaaaaaaaaaaATACAC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACat                <  2:3099461/123‑1 (MQ=255)
                                                                                                    attaaaaaaaaaaaaaaaaaaaaaaCCATATA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                 <  1:222754/123‑1 (MQ=255)
                                                                                                     taaaaaaaaaaaaaaaaaaaATCCC‑‑ATTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAAATAAAAACAAACTCTATTTATTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata               <  1:1511183/124‑1 (MQ=255)
                                                                                                      cacaAATAAAAAAAAAACAATCCCC‑‑TTAA‑‑‑AAATTAAAAAAAAAGAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACaaa               >  1:1784977/1‑126 (MQ=255)
                                                                                                        caAATAAAAAAAAAACAATCCCCTTAAA‑‑‑AATTAAAAAAAAAAAAAAAAAAAAAACTAAAAAAAAAATATATTTTTTAAAATAACAAATTATAACACATTTTATTTTTAAAAAAAGAAATTAAaataat             >  2:2973612/1‑128 (MQ=255)
                                                                                                        aaaaaaaaaaaaaaaaaaaaaCCCATTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataa              <  1:3246526/127‑1 (MQ=255)
                                                                                                        aaaaaaaaaaaaaaaaaATACACA‑‑TAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata            <  1:2011034/127‑1 (MQ=255)
                                                                                                           aTAAAAAAAAAACAATCCCCTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAA‑‑‑‑AAAAACAAAAACTTTTTATTAAAATTCCATATTAAACAACATTTTTTTTTTAAAATAAGATATTAAATTAATATTtatata    >  2:431221/1‑128 (MQ=255)
                                                                                                            taaaaaaaaaaaCAATCCCCTT‑‑‑A‑‑‑AAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAAAACTATTAATTAAAATTCAATATTTTAAAAAATTTTTTTTTTAAAATAAGATATTAAAAAAttatataaa      >  1:3127480/3‑126 (MQ=255)
                                                                                                             aaaaaaaaaaaaaaaACCC‑‑TTAA‑‑‑AAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatata        <  1:414404/127‑1 (MQ=255)
                                                                                                                aaaaaaaaaaaaCCCCTT‑‑‑A‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTa    <  2:977206/128‑1 (MQ=255)
                                                                                                                                |                                                                                                                        
AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAA‑‑‑AAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAAT  >  CP016816/202358‑202601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: