Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT98.1% 3641.3 / NA 1258intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (3/21);  new base T (808/426);  total (811/447)
Fisher's exact test for biased strand distribution p-value = 1.64e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1308 total aligned reads displayed.

TATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAA  >  CP016816/165091‑165344
                                                                                                                                 |                                                                                                                                
  tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                <  2:1419605/128‑1 (MQ=255)
  tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                >  1:2754864/1‑128 (MQ=255)
  tttACATTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                <  1:2561906/128‑1 (MQ=255)
    tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaa                                                                                                                              >  2:450670/1‑127 (MQ=255)
     aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                             <  2:611442/128‑3 (MQ=255)
     aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                             <  2:3088817/128‑3 (MQ=255)
     aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                             >  2:1763157/1‑126 (MQ=255)
      cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                            <  2:3880004/128‑1 (MQ=255)
        ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAAATATTAACTTTTTATATATTTTTTTAATATGAAAATTTTTAAAAaata                                                                                                                          >  2:2304370/1‑127 (MQ=255)
         tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                >  1:18468/1‑121 (MQ=255)
         tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                         >  2:3529033/1‑128 (MQ=255)
           aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                      >  2:2595717/1‑128 (MQ=255)
           aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                      >  1:3191566/1‑128 (MQ=255)
           aTTGTATTACTTTACTAGTTGATAATATTTAGTATCATTAATTTTTCGCAGTTGTATGAATTAGTAAGAATCTAAAACTAATATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                      <  1:2071015/128‑1 (MQ=255)
            ttGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaatt                                                                                                                     <  2:50683/128‑1 (MQ=255)
              gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                   >  1:2678483/1‑128 (MQ=255)
              gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                   <  2:2857268/128‑1 (MQ=255)
              gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTATTTATATGCAAATTTTTAAAAAATTAAtttt                                                                                                                   >  1:2987892/1‑127 (MQ=255)
                 ttactttactAGTTGATAATATTTAGTATCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTTTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAc                                                                                                                <  1:3813443/128‑1 (MQ=255)
                    ctttactAGTTGATAATATTTAGTTTCATTAATTTCTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                             <  1:1515524/128‑1 (MQ=255)
                    ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                             <  2:945068/128‑1 (MQ=255)
                    ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                             >  1:112776/1‑128 (MQ=255)
                    ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAAttt                                                                                                             >  1:2387828/1‑127 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                            >  2:3251741/1‑128 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                            >  2:1497148/1‑128 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                            >  1:3415625/1‑128 (MQ=255)
                      ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTa                                                                                                           >  2:2683087/1‑128 (MQ=255)
                       tactAGTTGATATTATTTAGTTTCATTAATTTATCTCTCTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                          <  2:2963514/128‑1 (MQ=255)
                       tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATTAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                          <  2:334254/128‑1 (MQ=255)
                          tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                       >  1:2420847/1‑128 (MQ=255)
                            gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAt                                                                                                     >  1:598911/1‑128 (MQ=255)
                                 taattTTTATTTTAATTAATTTATATTAATTTTTTTAATTATTAATAAAATAAAAATATTATATTTTTATATATTTTTTTTATATTAAAATTTTTAAAAAATTAAATTTAACTTGTAAAAAATAGCtt                                                                                               <  1:536456/128‑1 (MQ=255)
                                 taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                               >  1:1352754/1‑128 (MQ=255)
                                  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                              >  1:3075382/1‑128 (MQ=255)
                                  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                              >  1:203210/1‑128 (MQ=255)
                                  aataTTTAGTTTCATTAAATTATAGCAATTGGATGTAATTGTAATAAAAAAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAAATAATTTTAAATTGTAAAAAATAGCttt                                                                                              <  2:1812682/128‑1 (MQ=255)
                                   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                             >  2:395949/1‑128 (MQ=255)
                                   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                             >  1:3726014/1‑128 (MQ=255)
                                        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGCttttttttt                                                                                        >  2:3757204/1‑128 (MQ=255)
                                          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATATAttttttttttt                                                                                      >  1:2116474/1‑128 (MQ=255)
                                          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                      >  2:3467967/1‑128 (MQ=255)
                                           tttCATTAATTTATCGCACTTTTATTAATTATTAAGAAACTAAAACTATTATATTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAAAGCtttttttttttt                                                                                     <  1:3263474/128‑1 (MQ=255)
                                           tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                     >  1:3338971/1‑128 (MQ=255)
                                               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                 >  1:856916/1‑128 (MQ=255)
                                               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttatttttttt                                                                                 >  1:375451/1‑128 (MQ=255)
                                                ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttattt                                                                                >  1:1065492/1‑128 (MQ=255)
                                                    tttttatctattGTTTTAATTATTAATAAAATAAAAATATTATTTTTTTTTATATTTTTTTTATATTCAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATACCTTTTTTTTTTTTTTTTTTtat                                                                            <  1:2888534/118‑1 (MQ=255)
                                                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAATAAATAGCttgttttttattttttttaaa                                                                            >  2:3038378/1‑125 (MQ=255)
                                                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTAAATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttttta                                                                            >  2:3172566/1‑126 (MQ=255)
                                                     ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttttttttttaaat                                                                           >  2:2063339/1‑124 (MQ=255)
                                                       ttagaaattttATTAATTATTAAAAAAATAAAAATATTATATTTTTTTATATTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATg                                                                        <  2:2112246/118‑1 (MQ=255)
                                                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTatatttaaa                                                                       >  1:976951/1‑123 (MQ=255)
                                                          gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATgta                                                                      >  1:3458019/1‑126 (MQ=255)
                                                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAata                                                                  >  2:3823234/1‑128 (MQ=255)
                                                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtaataatttaaat                                                                  >  1:1602407/1‑117 (MQ=255)
                                                                  aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTTAAATTTAAATAAAAAt                                                             >  2:1583990/1‑128 (MQ=255)
                                                                  aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAATGAAAATATAAAt                                                             >  1:1186408/1‑128 (MQ=255)
                                                                   ttaataattaataaAATAAAAATATTATTTTTTTTTATATTTTTTTTATATGAAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATTTGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                            <  2:856916/120‑1 (MQ=255)
                                                                   tGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAATTTTTTAAAAAATTAATTTGAACTTGTAAAAATTAGATTTTTTTTTTTTTTTTTTTAT‑‑TGTAAATAAAAAttt                                                           >  1:2616999/1‑128 (MQ=255)
                                                                    gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATGGC‑‑TTTTTTTTTTTTTTTTTATATTGTAAATATAATtttt                                                          >  2:2453844/1‑128 (MQ=255)
                                                                       ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAAAATAATTTTTat                                                        >  2:1967989/1‑127 (MQ=255)
                                                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAATAATTAAAATAAAAATTTTat                                                        >  1:1671750/1‑127 (MQ=255)
                                                                          tttataaaataaaaataTTTTTTTTTTTTTTTTTTTTTTTTTATTTAAATTTTTTAAAAAATTATTTTTAAATTTTAAAAATATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                      <  2:2396022/123‑1 (MQ=255)
                                                                          ttaattaactaaaaataTTTTATTTTTTTATATTTTTTTTATATTTAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATATATTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                      <  1:2481426/122‑1 (MQ=255)
                                                                           taataaACAAAAAATATTTTTTTTTTATATATTTTTTTTATATTAAAATTTTTAAAAAAATAATTTGTACTTTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtt                                                   <  2:2211611/128‑1 (MQ=255)
                                                                           tAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTattta                                                     >  1:3121720/1‑124 (MQ=255)
                                                                            aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATATAAATATAAATTTtatttatt                                                   >  1:1399061/1‑128 (MQ=255)
                                                                              ttatataaaaataTTTTTTTTTTTTATATTTTTTTTATATTTAAATTTTTTAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtttt                                                 <  1:1285489/123‑1 (MQ=255)
                                                                              gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAATTTAAAAAAAAAATTTATTTAtttt                                                 >  2:3685281/1‑128 (MQ=255)
                                                                                       actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTAATTTATTTTTTTTTATaa                                        >  1:2323002/1‑128 (MQ=255)
                                                                                        atattttCTATTTAATTAATTTTTTTTTATTGAAAATTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                        <  2:725298/127‑1 (MQ=255)
                                                                                           ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTGTATAAGTAta                                   >  1:3668587/1‑127 (MQ=255)
                                                                                           ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTATTTTTTTTTATaatt                                      >  1:2795026/1‑126 (MQ=255)
                                                                                                  tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTAAATTTAAATTTTATTAATTTTTTTTTATAATTATAaaaaaa                             >  1:918679/1‑128 (MQ=255)
                                                                                                   ttatttatTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                           <  2:1042856/123‑1 (MQ=255)
                                                                                                   ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTATAAAATTTAAATATAAAATTTATTAATTTTTTTTTATAATTATATaaaaaa                            >  1:719889/1‑128 (MQ=255)
                                                                                                    tatatatTTTTTTTTTATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                           <  2:1351487/125‑1 (MQ=255)
                                                                                                     attattattttttttATGTATATTTTTAAAAAAATTATTTTAAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                           <  2:3426910/121‑1 (MQ=255)
                                                                                                     aGATATTTTTTGTTTATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                       <  1:1461200/128‑1 (MQ=255)
                                                                                                      tttatTTTTTTTTTATTTAAATTTTTAAAAAATTATTTTTAAATTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                         <  1:1964910/126‑1 (MQ=255)
                                                                                                      tttatTTTTTTTATATGTAAATTTTTTAAAAATTAATTTTTATTTTTATAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                         <  2:1882546/126‑1 (MQ=255)
                                                                                                      gATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAAGTATATaaaaaaaaaa                        >  1:3389532/1‑125 (MQ=255)
                                                                                                       atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTTTCTAATTATATAAAAAAATaa                        >  1:3532185/1‑127 (MQ=255)
                                                                                                       atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAATTGGAAATAAAAATTTTATGTATTTTTTTGTATATTTTTCTAAAAAAAttta                       >  2:1189059/1‑125 (MQ=255)
                                                                                                        tatTTTTTTTATAATCAAATTTTTAAATAATTAATTTTAACTTTTAAAAATTA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                      <  1:2281378/128‑1 (MQ=255)
                                                                                                        atatttttGTTTATTTAAATTTTTTAAAAATTAATTATTAATTTTTAAAAATATATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                       <  1:455326/125‑1 (MQ=255)
                                                                                                         atTTTTTGTTAATTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                       <  1:400667/128‑1 (MQ=255)
                                                                                                         atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTGTATAATTATATAAAAAAATATaaa                     >  1:1004419/1‑128 (MQ=255)
                                                                                                           ttttttTATATTAAAATTTTTAAAAATTTAATTTTAAATTTTAAAAATTAGTTTTTTTTTTTTTTTTTTTATATTGTAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                    <  2:3586706/128‑1 (MQ=255)
                                                                                                           tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAATTATAAATTTTAGTTTTTTTTTTTGATATTTTTATAAAAAAATaaaatg                    >  1:1752756/1‑126 (MQ=255)
                                                                                                             tttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATGTATTTTTTTTTATAATTATAaaaaaaaaaaaaaaat                   >  2:1117386/1‑126 (MQ=255)
                                                                                                               ttttttattaaaaTTTTTAAAAATTATATTTTAATTTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa               <  2:2778642/118‑1 (MQ=255)
                                                                                                               tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATTTAAATAAAATTTTTATTTATTTTTTTTTTAAATTTTATAAAAAATTATAAATTaaaa               >  1:802147/1‑128 (MQ=255)
                                                                                                               tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGTTATTTTTTTTTAAAATTAACTAAAAAAAAAAAAACTaaaa               >  2:165499/1‑128 (MQ=255)
                                                                                                                tttttatttaaaTTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa               <  2:31377/119‑1 (MQ=255)
                                                                                                                   tatTCAAATTTTTAAAAAATTTATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat            <  2:3732485/128‑1 (MQ=255)
                                                                                                                    attcaaaaTTTTAAAAAATTAATTTGTAAATTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat          <  1:2208898/123‑1 (MQ=255)
                                                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTTTTTAAGTATATAAAAAAATATAAAGAAAAAAatatat        >  1:3584810/1‑126 (MQ=255)
                                                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGAAAATTAAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATAAAAAGAAAAAAATAtttt       >  2:1279003/1‑126 (MQ=255)
                                                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATATTTTTTTTTTTATAAATATATAAAAAAATATAATTAAAAAAATATtttt      >  1:38935/1‑128 (MQ=255)
                                                                                                                                 |                                                                                                                                
TATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAA  >  CP016816/165091‑165344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: