BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,222	G→A	intergenic (+186/‑356)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,222	0	G	A	98.7%	3772.4 / 28.2	1251	intergenic (+186/‑356)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (806/419); minor base . (2/14); total (809/442)
Fisher's exact test for biased strand distribution p-value = 1.92e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1273 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTAAAAGAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349

cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTaaaaaa                                                                                                                                       >  1:3420449/1‑128 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAaat                                                                                                                                      >  2:3403407/1‑128 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAaat                                                                                                                                      <  1:3084607/128‑1 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAaatta                                                                                                                                    <  1:1956016/128‑1 (MQ=255)

    aaTTGGATCACTTTACTTGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAaattaa                                                                                                                                   <  2:2776487/128‑1 (MQ=255)

      ttGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAaattaatt                                                                                                                                 <  2:3717207/128‑1 (MQ=255)

        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTg                                                                                                                               <  1:9200/128‑1 (MQ=255)

         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGa                                                                                                                              <  2:3528733/128‑1 (MQ=255)

          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTTaa                                                                                                                             >  1:2760113/1‑128 (MQ=255)

             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑tt                                                                                                                         >  1:106355/1‑128 (MQ=255)

             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑tt                                                                                                                         >  1:3901575/1‑128 (MQ=255)

             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑tt                                                                                                                         >  1:2687174/1‑128 (MQ=255)

              ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTg                                                                                                                        <  2:945068/128‑1 (MQ=255)

              ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTg                                                                                                                        >  1:2905324/1‑128 (MQ=255)

              ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTg                                                                                                                        >  1:112776/1‑128 (MQ=255)

               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGt                                                                                                                       >  2:3251741/1‑128 (MQ=255)

               tttactAATTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAATAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAATAGTAATTTGAAC‑TTGt                                                                                                                       >  2:3682127/1‑128 (MQ=255)

                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTa                                                                                                                      <  1:2026026/128‑1 (MQ=255)

                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTa                                                                                                                      >  1:1501690/1‑128 (MQ=255)

                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATTAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTaa                                                                                                                     <  2:334254/128‑1 (MQ=255)

                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTaaaa                                                                                                                   >  1:41081/1‑128 (MQ=255)

                    tatttGATAATATTTAGTTTCATTAATTTATCGCAATTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTaaaaa                                                                                                                  <  1:2067387/125‑1 (MQ=255)

                       tttataataTTTAGTTTAATTAATTTATCTCAATTGTATTAATTATTAATAAAATAAA‑AATATTATATTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTTAAC‑TTTTAAAAAA‑Ta                                                                                                              <  2:3818709/125‑1 (MQ=255)

                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAATAAATTAATTTGAAC‑TTGTAAAAAA‑Ta                                                                                                              >  2:3130114/1‑128 (MQ=255)

                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑c                                                                                                          >  2:1890091/1‑128 (MQ=255)

                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTATAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑c                                                                                                          >  1:2571302/1‑128 (MQ=255)

                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttt                                                                                                      >  1:228357/1‑128 (MQ=255)

                              tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt                                                                                                    >  1:2803642/1‑128 (MQ=255)

                               atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt‑t                                                                                                  >  1:3815889/1‑128 (MQ=255)

                                tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt‑tt                                                                                                 >  2:241528/1‑128 (MQ=255)

                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt‑tttt                                                                                               >  1:1654214/1‑128 (MQ=255)

                                   aGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt‑ttttt                                                                                              >  2:1742581/1‑128 (MQ=255)

                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt‑tttttt                                                                                             >  1:1833345/1‑128 (MQ=255)

                                      ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt‑tttttttt                                                                                           >  2:3745247/1‑128 (MQ=255)

                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑A‑ttttt‑ttttttttt                                                                                          >  2:2699068/1‑128 (MQ=255)

                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt‑tttttttttt                                                                                         >  1:3671874/1‑128 (MQ=255)

                                          ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑ttttt‑tttttttttttt                                                                                       >  1:1340096/1‑128 (MQ=255)

                                                ttttgCAATTTTATGAATTATTAATAAAAATAA‑AATATTTTATTTTTATAATTTTTTTTTATA‑TTCAAATTTTTAAAAAATTAATTTTAAC‑TTTTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAAt                                                                              <  2:1044978/124‑1 (MQ=255)

                                                 aTCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTa                                                                                                                      <  2:443554/95‑1 (MQ=255)

                                                 aTCGCAATTGTATTAATTATTAATAAAATAAA‑AATATTTTATTTTTATTTATTTTTTTTATA‑TTCAAATTTTTAAAAAATTAATTTGAAA‑TTTTAAAAAA‑AA‑T‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATg                                                                             <  2:3944702/128‑1 (MQ=255)

                                                      aCTTTTTTGAATTATTAATAAAATAAA‑ACTATTTTATTTTTTTATATTTTTTGTATA‑TTTAAATTTTTTATAAATTAATTTGAAT‑TTTTTAAAAA‑‑A‑A‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAat                                                                        <  1:339300/128‑1 (MQ=255)

                                                       cTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTAT‑TTTTTTTTTTTtataatataaaaaa                                                                          >  1:808697/1‑118 (MQ=255)

                                                       cTTGTATGAAATATTAATAAAATATA‑AATATTATTTTTTTACATTTTTTTTGTATA‑TGCTAATTTTTAAAAAATTAATTTTAAA‑ATTTAAAAAA‑AA‑G‑C‑‑‑TTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAatat                                                                      <  1:3141251/128‑1 (MQ=255)

                                                          gTATTAATTTTTAATAAAATAAA‑ACTATTATTTTTTTATATTTTTTTTTTATA‑TTAAAATTTTTAAAAAATTAAATTTTAA‑TTTTAAAAAA‑TA‑T‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATaa                                                                    <  2:2117775/128‑1 (MQ=255)

                                                           tATTAATTATTTATAAAATAAA‑ACTATTATATTTTTTTATTTTTTTTTTATA‑TTTAAATTTTTATAAAATTTATTTTAAA‑TTTTAAAAAA‑TA‑T‑C‑TTTTTATTTTTTTTTTTTTTA‑‑TAATGGAAAtata                                                                     <  2:2851469/128‑1 (MQ=255)

                                                           tATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAAtttaaaaaaaaa                                                                   >  1:631401/1‑117 (MQ=255)

                                                           tATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTAGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTAA‑‑TAATTAAAAAATaaa                                                                   >  1:2486160/1‑128 (MQ=255)

                                                            ttttatttttAAGAAATTAAATATTTTTAT‑TTTTTAGATATTTTTTTTATA‑TGCAATTTTTTAAAAAATTAATTTTAAC‑TTGTAAAAAT‑TA‑G‑‑‑‑GTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAAtt                                                                 <  1:2622394/119‑1 (MQ=255)

                                                            aTGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑T‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑‑AATTTAAATAAAAAt                                                                  >  2:1583990/1‑128 (MQ=255)

                                                            aTGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑‑AATGAAAATATAAAt                                                                  >  1:1186408/1‑128 (MQ=255)

                                                             ttaataattaataaAATAAA‑AATATTATTTTTTTTTATATTTTTTTTATA‑TGAAAATTTTTAAAAAATTAATTTTAAC‑TTTTAAAAAA‑T‑‑T‑T‑GTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAAtt                                                                 <  2:856916/120‑1 (MQ=255)

                                                             ttaaTTATTTATAAACTAAA‑AATATTATTTTTTTATATATTTTTTTTATA‑TGCAAATTTTTAAAAAATTAATTTTAAA‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAAtt                                                                 <  1:3463381/126‑1 (MQ=255)

                                                                aTTAGTAATTAAATAAA‑ATTTATTTCTTTTTTTATTTTTTTTGTATT‑TGCAAATTTTTAAAAAAATAATTTTTAA‑TTTTAAAAAA‑AA‑‑‑‑‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAg                                                             <  2:2471313/128‑1 (MQ=255)

                                                                aTTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATTTAAATATAAATTTTa                                                              >  1:2428740/1‑128 (MQ=255)

                                                                  ttttaattaaataaa‑aattTTTTTTTTTTTTATTTTTTTTTTTTA‑TTAAAATTTTTAAAAAATTAATTTTTAA‑TTTTAAAAAA‑TT‑T‑T‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAg                                                             <  2:3084553/117‑1 (MQ=255)

                                                                  tAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATTAAAATATAAATTTTAgt                                                            >  1:3339342/1‑127 (MQ=255)

                                                                  tAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑‑TTT‑TTTTTTTTTTTTTTA‑‑TATTGTATATTTAAATTTTAGGt                                                           >  2:1127111/1‑128 (MQ=255)

                                                                  tAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑‑TTT‑TTTTTTTTTTTTTTA‑‑TAATTGAAATATAAATTTTAgtt                                                           >  2:1821015/1‑126 (MQ=255)

                                                                   attataaaaataaa‑aaaaTTTTTTTTTTTTATATTTTTTTTTTA‑TTTAAATTTTTAAAAAATTAATTTTTAA‑TTTTAAAAAATTT‑T‑T‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAg                                                             <  1:1458931/122‑1 (MQ=255)

                                                                   aGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTTT‑TTTTTTTTTTTTTTT‑‑AAATTTAAAAATAAATTTTatt                                                            >  1:1265482/1‑126 (MQ=255)

                                                                     taattaaattaa‑aataTTATCTTTTTATATTTTTTTTTTAAT‑TTCAAATTTTTAAAAAATTTATTTGAAC‑TTTTAAAAAA‑TT‑T‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTa                                                          <  1:3532717/118‑1 (MQ=255)

                                                                        ttaactaaa‑aaatTTATATTTTTTTTTTTTTTTTTTTTA‑TTCAAATTTTTAAAAAATTAATTTTTAA‑TTTTAAAAAA‑AA‑T‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTAtttt                                                      <  2:501662/126‑1 (MQ=255)

                                                                        ttaaaaaaa‑aataTTTTCTTTTTATATATTTTTTTTATA‑TGCAAAATTTTAAAAAATTAATTTTAAA‑TTTTAAAAAA‑TATGTT‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTAt                                                         <  1:3729370/122‑1 (MQ=255)

                                                                             taaa‑actaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATTAAAATATAAATTTTAGATATtttttttt                                                 >  1:3271094/1‑126 (MQ=255)

                                                                               aa‑actaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATTTAAATATAAATTTTATTTATttttttttat                                               >  1:3211947/1‑124 (MQ=255)

                                                                                  tatattatATTTTTATTTAATTTTTTTATA‑TTCAAATTTTTAAAAAATTTATTTTTAA‑TTGTTAAAAA‑‑A‑T‑T‑CTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  2:3586471/126‑1 (MQ=255)

                                                                                  actaTTATCTTTTTTTTTATTTTTTGTATA‑TGAAAATTTTTAAAAAATAAATTTGAAC‑TTGTAAAAAA‑T‑‑‑‑T‑TCTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                            <  1:3010515/128‑1 (MQ=255)

                                                                                  actaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑T‑C‑TTTTT‑TTTATTTTTATTATA‑‑‑AAT‑GAAATATAATTTTTAGGTATTTTTTTGTATAAg                                            >  2:3025006/1‑128 (MQ=255)

                                                                                  actaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTTT‑TTTTTTTTTTTTTT‑‑‑T‑ATGGAAATATAAATTTTATGTATTTTTTTGTCTaat                                            >  1:3754941/1‑127 (MQ=255)

                                                                                  actaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATTAAAATATAAATTTAATTTATTTTTTTTTATaa                                             >  1:2323002/1‑128 (MQ=255)

                                                                                   ttattatGTTTTTTTATATTTTTTTTATT‑TTATAATTTTTTAAAAAATAATTTGAACTTTGTAAAAAT‑TA‑‑‑‑‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                            <  1:2389355/127‑1 (MQ=255)

                                                                                   atattttCTATTTAATTAATTTTTTTTTATTGAAAATTTTAAAA‑AATTAATTTTAAA‑TTTTAAAAAA‑TA‑T‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  2:725298/127‑1 (MQ=255)

                                                                                    tattatCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGTAAATATAAATTTTAGTTAAtttttttaataag                                            >  1:3595158/1‑121 (MQ=255)

                                                                                      ttatCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTTT‑TATTTTTTTTTTTTATTTAATTTAAATAAAATTTTTATGTAATTTTTTTTCAAAg                                            >  1:1744209/1‑128 (MQ=255)

                                                                                       tatCTTTTTAGATATTTTTTGTATA‑TGCAAATATTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑‑‑‑ATTT‑TTTTTTTTTTTTTTA‑‑TAATTTAATAATAAATTTTATTTAATttgttttatatttaaat                                       >  1:740966/1‑116 (MQ=255)

                                                                                         tttttttttttatTTTTTTTTTT‑TTTAAATTTTTAAAAAAATAATTTTTAA‑TTTTAAAAAA‑TT‑T‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                       <  2:2081171/118‑1 (MQ=255)

                                                                                            ttttttatatTTTTTTTATA‑TTTAAATTTTTTAAAATTTTATTTTAAA‑TTTTAAAAAA‑TA‑T‑T‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaa                                    <  1:867427/122‑1 (MQ=255)

                                                                                            ttttAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATGTAAATATAAATTTTATGTATTTTTTTTAATAATTATATaaaa                                   >  1:1395249/1‑128 (MQ=255)

                                                                                               tAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TATTTTAAATATAATTTTTATGTATTTTTTTTTATAATTATATaaaaaaa                                >  1:545491/1‑128 (MQ=255)

                                                                                                 gATTTTTTTTGTTTT‑TGAAAATTTTTAAATATTTATTTTTTTA‑TTTTAAAAAA‑AA‑G‑‑‑‑GTTT‑TTTTTTTTTTTTTTA‑‑TAATGGTAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  2:1975238/128‑1 (MQ=255)

                                                                                                  atatTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑‑TTT‑TTTTTTTTTTTTTTA‑‑AATTGGAAATAAAAATTTTATGTATTTTTTTGTATATTTTTCTAAAAAAAttta                            >  2:1189059/1‑125 (MQ=255)

                                                                                                   tatTTTTTGTTTA‑TGCAAATTTTTAAAAAATTAATTTTTAA‑TTGTTAAAAA‑TA‑‑‑T‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                            <  1:2098498/128‑1 (MQ=255)

                                                                                                   tatTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTATA‑‑TAATTTAAAAATAAATTTTATATATTTTTTTTTATAATTAAAAAAAAAAATaaa                            >  2:3171300/1‑126 (MQ=255)

                                                                                                    tttttttttttA‑TTTAAATTTTTAAAAAATTTATTTTAAA‑TTTTTAAAAA‑TT‑T‑T‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                            <  2:2216350/127‑1 (MQ=255)

                                                                                                    atTTTTTGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑‑TTT‑TTTTTTTTTTTTTTA‑‑TAATTTAAATATAAATTTTAGTTATTTTTTTGTATAATTATATAAAAAAATATaaa                          >  1:1004419/1‑128 (MQ=255)

                                                                                                      ttttttTATA‑TTTAAATTTTTAAAAAATTAATTTTAAA‑TTTTTAAAAA‑TA‑T‑A‑GTTTT‑TTTTTTTTTTTTATA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                          <  2:295953/128‑1 (MQ=255)

                                                                                                      tttttGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATTTAAATATAAATTTTATGTATTTTTTTTTATAATTATAAAAAAAAATAaaaat                         >  2:3467749/1‑127 (MQ=255)

                                                                                                        ttttttttttttaaaTTTTTTAAAAATTTATTTTTAA‑TTTTAAAAAA‑TA‑T‑T‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                         <  1:979930/116‑1 (MQ=255)

                                                                                                         ttttttttttaaaaTTTTTAAAAAATTAATTTTTAA‑TTTTAAAAAA‑T‑‑‑‑T‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                      <  2:2354649/117‑1 (MQ=255)

                                                                                                          tGTATA‑TGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑‑TTT‑TTTTTTTTTTTTTTA‑‑TAATGAAAATATAAATTTTAGTTATTTTTTTTTAAAATTAACTAAAAAAAAAAAAACTaaaa                    >  2:165499/1‑128 (MQ=255)

                                                                                                              ta‑tGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATTGAAATAAAAATTTTATGTATTTTTTTTTTTAATTATATAAAAAAAAAAAAAGTaaaaaaa                 >  2:1494891/1‑127 (MQ=255)

                                                                                                               tatttataTTTTTAAAAAATTATTTTTAAA‑TTTTAAAAAA‑TA‑‑‑T‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:464833/121‑1 (MQ=255)

                                                                                                               attcaaaaTTTTAAAA‑AATTAATTTGTAA‑ATTTAAAAAA‑TA‑‑‑‑‑TCTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat               <  1:2208898/123‑1 (MQ=255)

                                                                                                               a‑tGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑CTTTTTT‑TTTTTTTTTTTTTTT‑‑TAATGGAAATATAATTTTTTTTTATTTTTTTTTATAATTTAATAAAAAAATTTAAATTaaaaaa                  >  2:1419048/1‑128 (MQ=255)

                                                                                                               a‑tGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTTT‑TTTTTTTTTTTTTTT‑‑TAATGTAAATATAAATTTTTTTTTTTTTTTTTTCTATTTATTTTAAAAAATTAAATGTaaaaaaa                 >  1:1596289/1‑127 (MQ=255)

                                                                                                                 tGCAAATTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATGTAAATATAAATTTTATTTATTTTTTTGTATAATTAAATAAAAAAATATAAAATAAAAAatat               >  2:589630/1‑128 (MQ=255)

                                                                                                                    aaaTTTTTAAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑TTTT‑TTTTTTTTTTTTTTA‑‑TAATGAAAATTAAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATAAAAAGAAAAAAATAtttt            >  2:1279003/1‑126 (MQ=255)

                                                                                                                      aTTTTTTAAAAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑‑‑‑TT‑TTTATTTTTTTTTTA‑‑TAATTATAATATAAATTTTAGGTATTTTGTTGTCTAAGTTACTAAAATAATATATAGAAAAAAATTTTGtttta        >  2:2599279/1‑125 (MQ=255)

                                                                                                                       ttttAAAA‑AATTAATTTTAAA‑TTTTAAAAAA‑AT‑T‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa         <  2:3863333/128‑1 (MQ=255)

                                                                                                                            aaaaaaTTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑CTTTTTT‑TTTTTTTTTTTTTTA‑‑TAATTGAATTATAAATTTTTTTTTTTTTTTTGTATAAATATAT‑AAAAAATATAAATGAAAAAATATTTTTaaaata     >  2:1344405/1‑126 (MQ=255)

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAA‑ACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTAAAAGAATTAATTTGAAC‑TTGTAAAAAA‑TA‑G‑C‑TTTTT‑TTTTTTTTTTTTTTA‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`