Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816437,8804.T42.4% 94.5 / inf 415intergenic (‑184/+238)JCVSYN2_01985/JCVSYN2_01990glycosyl transferase/ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base . (114/125);  new base T (120/56);  total (234/181)
Fisher's exact test for biased strand distribution p-value = 3.81e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-03

Only 100 of 719 total aligned reads displayed.

TGAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTAGATTT  >  CP016816/437753‑438003
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tGAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑g                                                                                                                                                            >  2:947135/1‑128 (MQ=255)
tGAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACATCCATATTACATATAGAGTTT‑‑‑AT‑‑‑‑g                                                                                                                                                            >  2:1523429/1‑128 (MQ=255)
 gAAACTTATTTTTGTAGTTTTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATAGTATTTTTAAAATAAATATTTTAGGAAATGAAGAAAAGCAAAATCCTACATAGTACCTATAGAGTTT‑‑‑AT‑‑‑‑G‑‑‑‑‑‑a                                                                                                                                                     >  2:1399947/1‑128 (MQ=255)
 gAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGATTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑a                                                                                                                                                     >  2:3178971/1‑128 (MQ=255)
 gAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑a                                                                                                                                                     >  1:1382168/1‑128 (MQ=255)
   aaCTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAATTATTTTAGGAAATGAAGCAAAGCAAAAACCTCAATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ata                                                                                                                                                   >  2:1742990/1‑128 (MQ=255)
   aaCTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ata                                                                                                                                                   >  2:2911375/1‑128 (MQ=255)
     cTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAATCCTCCATATTCACTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atata                                                                                                                                                 >  2:815257/1‑128 (MQ=255)
      ttATTTTTGGAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAACGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatat                                                                                                                                                >  2:1336829/1‑128 (MQ=255)
        aTTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat                                                                                                                                              >  2:712745/1‑128 (MQ=255)
         tttttGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCAATATTACCTATAGAGTTT‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atttatat‑‑‑‑a                                                                                                                                         >  1:767325/1‑128 (MQ=255)
         tttttGTAGTTGTAAGTTTCATTTTTAATAAATAATAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a                                                                                                                                         >  1:80634/1‑128 (MQ=255)
            ttGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tat                                                                                                                                    >  1:2863354/1‑128 (MQ=255)
             tGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tata                                                                                                                                   >  1:86520/1‑128 (MQ=255)
                aGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatat                                                                                                                                >  1:271043/1‑128 (MQ=255)
                  ttttatgattttttttttttAAATATTTTATATATAAATTTTTTTTTTAAAATATATATTTTATTTTATTAATAAAAACTAAAACCTCCATATTAAATATATAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatat                                                                                                                              <  1:1342227/116‑1 (MQ=255)
                  ttttaatattCATTTTTAATAAATATTAGAAATAAAAATAGTATTTTTAAAATAAATATTTTAGTAAATGAATCAAAGCAAAAACAACCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatat                                                                                                                              <  2:562921/120‑1 (MQ=255)
                    gttaGTATCATTTTTAATAAATATTAGAAATAAAAATTTTATTTTTAAAAAATATATTTTATTATATGAATCAAATAAAAAACCTCCATATAACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑t                                                                                                                      <  2:665841/125‑1 (MQ=255)
                    atAATTTTCATTTTTAATAAATATTATAAATATAAATTTTATTTTTAAAATAAATATTTTATTAAATGAATCAAAGAAAAAAACTAAATATTTACTATATAGTTA‑‑‑CT‑‑‑‑T‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑t                                                                                                                      <  1:1236407/127‑1 (MQ=255)
                       aGTTTTTTTTTTAATAAATATTATAAATAAAAAATTTATTTTTAAAATAAATATTTTAGTATATGAATCAAAGAAAAAAACTCAATATTAAATATATAGTTA‑‑‑TT‑‑‑‑t‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tata                                                                                                                   <  1:1483408/128‑1 (MQ=255)
                        ttttCATTTTTAATAAATATTATAAATAAAAATTTTATTTTTAAAATAAATATTTTAGTAAATGAAGCAAACCAAAAACATCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat                                                                                                                  <  2:1567616/127‑1 (MQ=255)
                         ttttATTTTTTATATATATTATATATAAAAATTTTTTTTTTAAAATAAATATTTTATGATATTTAGCAAATCAAAAACCTCCATATTACCTATATAGTTA‑‑‑TT‑‑‑‑T‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑a                                                                                                           <  1:966473/128‑1 (MQ=255)
                          ttCATTTTTAATAAATATTATAAATAAAAATTGTATTTTTAAAATAAATATTTTATGAAATGAAGCAAATCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑at                                                                                                          <  1:3152333/128‑1 (MQ=255)
                           tttttttttATATATATTATAATTATAAATTGTATTTTTATAATTAATATTTTATTTAATTTATAAAATATAAAACATCGATATTTTCTATAGAGTTA‑‑‑AT‑‑‑‑T‑‑‑‑‑‑ttatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑ata                                                                                                         <  1:1080727/125‑1 (MQ=255)
                           taaTTTTTAATAAATATTATATATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATTAATCAAAGAAAAAACCTCCATATAAACTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑ata                                                                                                         <  1:43106/126‑1 (MQ=255)
                           tCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTATGAAATGAATCAAAGCAAAAACCTCCATATAACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑ata                                                                                                         <  2:2096035/128‑1 (MQ=255)
                            taTTTTTTATAAATATTATATATATAAATTTTATTTTTAAAATAAATATTTTATTTAATGAAGCAAAGAAAAAACCTCAATATTAACTATATAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atat                                                                                                        <  1:1880495/127‑1 (MQ=255)
                            cATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atat                                                                                                        >  2:858701/1‑128 (MQ=255)
                             ttttttAATATATATTATAAATAAAAATTTTATTTTTAAAATAAATATTTTTTTAAATTAATAAAATAAAAAAACTCAATATATACTATAGATTTA‑‑‑TA‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atata                                                                                                       <  2:2648509/127‑2 (MQ=255)
                             aTTTTTTATAAATATTATAAATAAAAATTTTATTTTTAAAATATATATTTTATTAAAAGAATCAAATCAAAAACCTCCATAAAAACTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atata                                                                                                       <  2:2281659/128‑2 (MQ=255)
                               ttttTATAAATATTAGAAATAAAAATATTATTTTTAAAATAAATATTTTAGTAAATGTAGCAAAGCAAAAACCTCAATATTACCTATAGAGATA‑‑‑AT‑‑‑‑T‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atatata                                                                                                     <  2:3045011/128‑4 (MQ=255)
                                tttAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atataata                                                                                                    >  2:1196961/1‑124 (MQ=255)
                                tttAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atataaga                                                                                                    >  1:1730351/1‑124 (MQ=255)
                                tttAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGAAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atatatat                                                                                                    >  1:1813316/1‑124 (MQ=255)
                                 tttataaataTTATATATAAATATTTTTTTTTTAAAATAAATATTTTTTTAAATTTATAAAATAAAAAAACTCTATATAATATATATAGATT‑‑‑TT‑‑‑‑T‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atatatata                                                                                                   <  1:65135/125‑6 (MQ=255)
                                 ttAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTATGAAATGAATCAAATCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatatgcaagcgat                                                                                                         <  2:894443/128‑10 (MQ=255)
                                  taataaATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGATGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatatgcaagctatc                                                                                                        >  2:2573431/1‑118 (MQ=255)
                                   aataaataTTAGAAATAAAAATTGTATTTTTATAATAAATATTTTATGAAATTAAGAAAATAAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atatatatgca                                                                                                 <  2:243717/128‑8 (MQ=255)
                                   aataaataTTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATTAATCAAATCAAAAACCTCCATATTAACTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atatatatgca                                                                                                 <  2:1484782/128‑8 (MQ=255)
                                       aataTTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑atatatat‑‑‑‑a‑‑tatatatata‑‑‑‑‑‑tatat‑‑‑‑‑‑atatatatgaacgag                                                                                             >  1:2949263/1‑117 (MQ=255)
                                           ttATAAATAAATATTTTATTTTTAAAAAATATATTTTATTAAATCAATAAAATAAAAAATCTATATATTATATATAGATTTAT‑‑ATA‑‑‑T‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGc                                                                                      <  1:170403/128‑1 (MQ=255)
                                              gAAATAAAAATTGTATATTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAAACTCAATATTAACTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATaaa                                                                                 <  2:3245631/128‑1 (MQ=255)
                                               aaaTAAAAATTTTATTTTTAAAATAAATATTTTAGTAAATGAAGCAAATCAAAAACCTCCATATTACATATATAGATA‑‑‑AT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATaa                                                                                  <  2:1667547/128‑1 (MQ=255)
                                               aaaTAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCAt                                                                                    <  2:1827721/128‑1 (MQ=255)
                                                 aTAAAAATTTTTTTTTTTAAATAAATATTTTATTTTATTAATAAAATAAAAAAACTCTATATTATATATAGAGTTA‑‑‑ATTATAT‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATaa                                                                                  <  1:3080297/128‑1 (MQ=255)
                                                  tAAAAATTGTATTTTTAAAATAAATATTTTAGTAAATGAAGCAAAGCAAAAACCTCAATATTAACTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATgg                                                                             <  1:1579436/128‑1 (MQ=255)
                                                    aaaaTTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCTATCAGAATAAAATGGTg                                                                           >  1:5020/1‑128 (MQ=255)
                                                     aaaTTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑TAA‑A‑GCGATCACAATAAAATgtt                                                                            >  1:1980448/1‑126 (MQ=255)
                                                      tattttattttTAAAATAAATATATTATTATATCAATAAAATCAAAAACCTCCATATTATATATAGAGTTA‑‑‑ATTA‑‑T‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTg                                                                           <  2:145091/127‑1 (MQ=255)
                                                            aTTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGCTCAGCATAAAATGCTGatag                                                                       >  1:3222784/1‑125 (MQ=255)
                                                            aTTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCATCATAAAATGGTGAAAt                                                                       >  1:1415851/1‑128 (MQ=255)
                                                            aTTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GAT‑A‑GCTATCAGCATAAAATGGTGATAttt                                                                     >  1:219457/1‑128 (MQ=255)
                                                            aTTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTACATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATATATATCA‑ACGCTATCAGAATAAAATGGTGAAAt                                                                       >  2:2538616/1‑128 (MQ=255)
                                                             tttttAAAATAAATATTTTAGTAAATGAATCAAAGAAAAAACCTCCATATTAAATATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑‑‑ATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATc                                                                <  1:2808346/128‑1 (MQ=255)
                                                              ttttAAAATATATATTTTAGTAAATTTATCAAATAAAAAAACTACATATTACATATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GTA‑A‑GCGATCAGCATAAAATGGTGAAAttt                                                                     <  2:350831/128‑1 (MQ=255)
                                                              ttttAAAATAAATATTTTATGAAATCAATCAAAGAAAAAACCTCCATATTACCTATAGAGATA‑‑‑ATTATAT‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAAttt                                                                     <  1:201775/128‑1 (MQ=255)
                                                               tttAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑‑TA‑‑‑‑‑‑A‑A‑TCGATCATAATAAAATTGTGAAATTTAGATATGAg                                                            >  2:3098040/1‑128 (MQ=255)
                                                                 tAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GAA‑A‑GATATCAAAATAAAATGGTTAAATTTAGa                                                                  >  2:1873361/1‑128 (MQ=255)
                                                                   aaataaaaATTTTATGATATGAATCAAATCAAAAAACTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATc                                                                <  2:2612931/128‑1 (MQ=255)
                                                                   aaaaaaatATTTTAGTAAATTAATAAAAGAAAAAACCTCAATATTATCTATAGAGTTA‑‑‑ATTATAT‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATc                                                                <  2:783478/128‑1 (MQ=255)
                                                                    aatatataTTTTAGTATATTTAGCGAATCAAAAAACTCTATATTATCTATAGAGTTATTTATA‑‑‑T‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCt                                                               <  1:164057/128‑1 (MQ=255)
                                                                    aataaataTTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCT‑A‑GCGATCAGCATAAAATTGTGAAATTTAGATCTGa                                                             >  2:2970615/1‑128 (MQ=255)
                                                                       taataTTTTATGAAATGAAGAAAAGCAAAAACCTCAATATTACATATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAg                                                            <  2:2333631/127‑1 (MQ=255)
                                                                         atatTTTATGAAATGTAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGt‑t                                                         <  1:2552666/128‑1 (MQ=255)
                                                                         atatTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑TCTATCAGAATAAAATGGTGAAATTTATATCTGAGt‑t                                                         >  1:2918047/1‑128 (MQ=255)
                                                                           atTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATATATAA‑‑ATAT‑‑‑GCA‑A‑GCGATCAAAATAAAATTGTGAAATATAGATCTGAGt‑ttg                                                       >  1:2120180/1‑126 (MQ=255)
                                                                             tttAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GAA‑A‑GCGATCAGCATAAAATGGTGATTTATAGATCTGAGT‑TG‑GTc                                                    >  1:2999285/1‑128 (MQ=255)
                                                                              ttAGTAAATTAATAAAATAAAAAAACTCTATATTAACTATAGATATA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTaaa                                               <  2:532683/128‑1 (MQ=255)
                                                                                  gAAATGAAGAAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTaaa                                               <  2:2195257/128‑1 (MQ=255)
                                                                                   aaaTGACTCAAATCAAAAACCTCTATATTAACTATAGAGTTA‑‑‑AT‑‑‑‑‑‑‑‑‑‑‑‑‑‑TATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGtt                                      <  1:2573431/128‑1 (MQ=255)
                                                                                     aTGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATATATAAA‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GAA‑A‑GCGATCATCATAAAATAGTGATATTTATATCTGAGT‑TG‑GTATTATAttt                                            >  2:1615376/1‑128 (MQ=255)
                                                                                       gAATCAAATCAAAAAACTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATATATATTTATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAAttt                                            <  2:1048149/128‑1 (MQ=255)
                                                                                        aagcaaatcaAAAAACTCCATATTATCTATAGAGATA‑‑‑ATTATAT‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTa                                         <  2:1375990/128‑1 (MQ=255)
                                                                                        aagcaaatcaAAAAACTACATATTATCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTa                                         <  2:1981868/128‑1 (MQ=255)
                                                                                         atcaaagcaaaAAACTACATATTATCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAg                                        <  2:2848797/126‑1 (MQ=255)
                                                                                         agcaaagcaaAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATATATATCATA‑GCGATCAGCATAAAATTTAGAAATTTAGATCTGAGT‑TG‑GTCTGAAATTTGTat                                        >  1:699992/1‑127 (MQ=37)
                                                                                          tcaaatcaaaAAACTACATATTAACTATAGAGTTA‑‑‑ATTATAT‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGt                                       <  2:2706228/127‑1 (MQ=255)
                                                                                          gcaaagaaaaCACCTACATATTATATATAGAGTTA‑‑‑TT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGttt                                     <  1:1002922/128‑1 (MQ=255)
                                                                                           caaatcaaaAACATCCATATTAACTATAGAGTTA‑‑‑AT‑‑‑‑‑‑‑‑‑‑‑‑‑‑TATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGTTtttattta                              <  1:3098040/128‑1 (MQ=255)
                                                                                            aaagcaaaAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑ATTTATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GAGATCAGCATAAATGGGTGAATTTTAGATCTGATT‑TG‑GTCTATAATTTGtttttttt                                   >  1:375251/1‑128 (MQ=255)
                                                                                              agcaaaAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GAGCTCAGAATAAAATGTTGATATTTAGATCTGAGT‑TG‑GTCTTAAATGTGTAGTTtatat                                 >  2:2038319/1‑128 (MQ=255)
                                                                                               gcaaaAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GAA‑C‑GCGCGCAGCAGATAATGGGGAGAATTAGAGCTGAGTGTGTGTTATATATTTATAGTTtttata                                >  2:432452/1‑127 (MQ=255)
                                                                                                caaaAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GAA‑A‑GCGATCAGCATAAAATTGTTAAATATAGATCTGAGT‑TT‑GTATTTAAtttttattttttat                                 >  1:2269893/1‑128 (MQ=255)
                                                                                                  aaaaCCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGTTTTTATTTATAg                           >  2:3067574/1‑128 (MQ=255)
                                                                                                  aaaaCCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑‑‑ATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATc                                                                >  1:115221/1‑91 (MQ=255)
                                                                                                   aaaCCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATATATATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGTTtttatt                                >  2:1254797/1‑128 (MQ=255)
                                                                                                   aaaCCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GAGATCAGAATAAATTGGTGATATTTATATCTGAGT‑TG‑GTGTTAATTTTGTAGTttttttttt                              >  1:2429787/1‑127 (MQ=255)
                                                                                                   aaaCCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGTTTTTATTtata                            <  2:1115614/128‑1 (MQ=255)
                                                                                                    aaCCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑CCA‑C‑GCGATCAGCATAAAATTGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTATAGTTTttatttat                             >  1:1167846/1‑128 (MQ=255)
                                                                                                       cTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTATTAAATTTGTAGTTTTTATTTATAGt                          >  1:2371998/1‑128 (MQ=255)
                                                                                                       cTCCATATTACCTAGAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GGGATCAGGATAAAATGGTGATATTTAGATGTGAGT‑TG‑GTGTTAATTTTGTAGTTTTTATATATAGt                          >  1:2633036/1‑128 (MQ=255)
                                                                                                        tCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GAA‑A‑GCGCTCAGCATAAAATGGTGAAATATATATATGAGT‑TT‑GTCTTAAATTTTTATTTTTTATATATAGTTtata                     >  1:619887/1‑128 (MQ=255)
                                                                                                         ccATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAATATGGTGAAATATAGATCAGAGT‑TG‑GTGTTAAATTTGTAGTTTTTATATATATTTtat                      >  1:3284564/1‑128 (MQ=255)
                                                                                                         ccATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATATATATAAATAT‑‑‑AAA‑A‑GCTATAATAATAAAATTGTGAAATATATCTCTGAGA‑TT‑GTCTAAAATTATTAGTTTTtatatatatt                          >  2:1923884/1‑126 (MQ=255)
                                                                                                           atatTACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCTATCAGAATAAAATGGTGAAATATAGATCTGATT‑AT‑GTCTTAGATTTCTAGTTTATTTATATAGTatat                      >  2:600180/1‑128 (MQ=255)
                                                                                                              ttACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCTATCAGCATAAAATTGTGAAATTTATATATGAGT‑TT‑GTCTTAAATTTTTATTTTTTATATATATTTTATaaa                   >  1:561683/1‑128 (MQ=255)
                                                                                                              ttACCTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑ACA‑A‑GCGATCAGAAAAAAAAGGTGATATTTAGATCTGAGT‑TG‑GTGATAAATATATATTTTTTATTTATAGTTTATaaa                   >  2:2017491/1‑128 (MQ=255)
                                                                                                               tACCTATAGAGTTA‑‑‑AT‑‑‑‑GAT‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GAA‑A‑GCGATCAAAATAAAATTGTGAAATATATATCTGAGT‑TG‑GTCTTAAATTTGTAGTTTTTATTTATATTTTATaattat                >  2:382835/1‑124 (MQ=255)
                                                                                                                 ccTATAGAGTTA‑‑‑AT‑‑‑‑GATAT‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCATCATAAAATTGTGAAATTTATATCTGAGT‑TG‑GTCTTAAATTTGTATTTTTTATTTATATTTTATAAAttt                >  2:2151069/1‑128 (MQ=255)
                                                                                                                     taGAGTTT‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAAttt        <  1:2516245/128‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                      
TGAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTA‑‑‑AT‑‑‑‑G‑‑‑‑‑‑ATATATAT‑‑‑‑A‑‑TATATATATA‑‑‑‑‑‑TATAT‑‑‑‑‑‑ATAT‑‑‑GCA‑A‑GCGATCAGCATAAAATGGTGAAATTTAGATCTGAGT‑TG‑GTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTAGATTT  >  CP016816/437753‑438003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑