Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.71.6% 282.4 / inf 297intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (9/64);  new base . (71/113);  total (95/202)
Fisher's exact test for biased strand distribution p-value = 2.38e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

Only 100 of 331 total aligned reads displayed.

AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATAC  >  CP016816/202358‑202612
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 atatAGTGTAAAAAATATTTTTAAGGTTAATTAAAAAACCATAAAATTAAAAATTCTTAAAAAGTAGAACCACGTGATAAATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtt                                                                                                                                 <  2:848162/125‑1 (MQ=255)
  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATATGTTTATCTTCACAAATAAAAAAAAAACAATCCACtaa                                                                                                                                >  2:2630139/1‑126 (MQ=255)
   ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCCtaaa                                                                                                                               >  1:2725786/1‑125 (MQ=255)
      gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACACCtaaaaaa                                                                                                                            >  2:2743476/1‑122 (MQ=255)
           aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑Ttaaaaattaaaa                                                                                                                   >  1:3127113/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCAAC‑‑TTAAAAAATaaaaaaaa                                                                                                               >  2:2602427/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCTTGATCTATTTTTTTATATATATATCATCACAAATAAAAAAAAAAAAAATCAACTTAAAAAATaaaaaaa                                                                                                                >  2:449136/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaa                                                                                                               >  1:2787862/1‑128 (MQ=255)
                    gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaa                                                                                                           >  1:2483325/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAATACC‑‑AATAAAAATTaaaaaaaaaaaaa                                                                                                          >  1:903937/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                          >  1:2611381/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAAAAATAAAAAAAAAACAATCCC‑‑‑ATAAAAAATaaaaaaaaaaaaaa                                                                                                         >  1:1054519/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACCAATCCCCTT‑‑‑AAAAATaaaaaaaaaaaaaaa                                                                                                        >  1:1764357/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaa                                                                                                         >  1:317625/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTATATTTGTTTATCTTCACAAATAAAAAAAAAACAATCAAC‑‑TTAAAAAATaaaaaaaaaaaaaa                                                                                                         >  1:1179594/1‑128 (MQ=255)
                       taGGTTAATTAAAAATCAATAAAATTTAATAATCTTATAAACTACAACCCCGTTATCTATTTTTTTATTTTTTTATCTTAAAAAAAAAAAAAAAAACAATCCCCTTT‑‑AAAATTaaaaaaaaaaaaaaa                                                                                                        <  2:1855355/127‑1 (MQ=255)
                       aattttaattaaAATAAAATAAAATTAATAATTCTTATAATTTATATCCTTTTTATCATTTTTTTTTTTTGTTTATTTTCACAAATAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                       <  1:1951399/124‑1 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑‑TAAAAAATaaaaaaaaaaaaaaaa                                                                                                       >  1:3032958/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                        >  2:2800029/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAAAAATAAAAAAAAAAAAAATCACATaaaaaaaaaaaaaaaaaaaaaa                                                                                                          >  1:1889445/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                       >  1:2813746/1‑128 (MQ=255)
                        aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                       >  1:2929702/1‑128 (MQ=255)
                        aGGTTAATTAAAATACCATAAACTTAATAATTCATATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAACAAT‑‑CAAATTAAAAATTaaaaaaaaaaaaaaaa                                                                                                       >  2:1924852/1‑128 (MQ=255)
                          tttaaataaAAAAAAATAAAATTAAAAATTTTTAAAATTTATATACTCTTTTTATATTTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                     <  2:2758206/127‑1 (MQ=255)
                          gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaaaaaaaaaaaaaa                                                                                                    >  1:3222302/1‑128 (MQ=255)
                           tttattaaAATAAAAAAAAATTAATAATTATTATAATTTATATCATCGTGTTTTATTTTTTTATTTTTTTATATTAACAAAAAAAAAAAAAACAAAACCCTTT‑‑AAAATTaaaaaaaaaaaaaaaaaaa                                                                                                    <  2:2888397/125‑1 (MQ=255)
                           ttaatttaAAAAACATAAAATTAAAAATTATTATAATTTATATCCTCTTTAATTTTTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                     <  2:241237/128‑1 (MQ=255)
                           gttattaaAAAAACATTAAATTAATAAATAATATAATTTATAACCTATTGAAATATTTTTTTATTTTTTTATCATCAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAAATaaaaaaaaaaaaaaaaaaa                                                                                                    <  2:1462272/125‑1 (MQ=255)
                            taattaaAATAACATAAACTTAATAATTCTTATAATTTATATACTAGTTATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAAAAAT‑‑CAAATTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                                   >  2:527698/1‑128 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                                <  2:320236/101‑2 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACACATTAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  1:2681280/1‑128 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATAAC‑‑ATAAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  1:1504950/1‑128 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAATCAACTTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                                   >  2:2572127/1‑128 (MQ=255)
                              attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCACTTAAAAATTATaaaaaaaaaaaaaaaaaaaa                                                                                                   >  2:418402/1‑128 (MQ=255)
                               ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACACC‑‑TTAAAAAATaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  1:2961128/1‑126 (MQ=255)
                               ttaaAATACCATAAACTTAATAATTCTTATAATCTAGATCATCGAGATCTATATTTTTATTTTTTTATCTTCAAAAATAAAAAAAAAACAA‑‑ACAAATAAAAAATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  2:2723145/1‑126 (MQ=255)
                                taaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATAAC‑‑AATAAAAAATaaaaaaaaaaaaaaaaaaaaaaaa                                                                                               >  2:383668/1‑125 (MQ=255)
                                 aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  1:2636871/1‑124 (MQ=255)
                                 aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  2:1746793/1‑124 (MQ=255)
                                 aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCAATTTTTTTATTAGATTATCTTCACAAATAAAAAAAAAAAAATCCC‑‑ATTAAAAAATaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  1:206241/1‑124 (MQ=255)
                                  aaaaataaaaaaaaTTAATAATTATTATAATTTATATAAAATTTATAAATTTTTTTATTTTTTTAACATAAAAAAAAAAAAAAAAACAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTaaa                                                                                             <  2:961785/118‑1 (MQ=255)
                                   aaaaaaaaaaaaaTTAATAATTTTTAAAATTTATAACCTCTTTAATTATTTTTTTATTTTTTATTTTTTAAAAAAAAAAAAAAAAAAAACCCATTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTa                                                                                               <  1:716904/119‑1 (MQ=255)
                                   aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAATaaaaa                                                                                           >  1:1747534/1‑128 (MQ=255)
                                    aataaaTAAAATTAATAATTAATATAATTTATATCATCGTTATATATTTTTTTTTTTTTTTTAATTCAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaac                                                                                          <  2:1120595/123‑1 (MQ=255)
                                    aaaaacaaaaaCTTAATAAATATTAAAATTTACAACCACGTTATCAATTTTTTTAATTTTTTAACATAAAAAAAAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTaaaa                                                                                            <  2:3058508/120‑1 (MQ=255)
                                     tACCAAAAAAATAAAAATTTTTATAAATTATTACCTCTTTATATATTTTTTTATTTTTTTATATTCAAAAATAAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTaaaaa                                                                                           <  2:2695730/128‑1 (MQ=255)
                                     aaaaaaaaaaaTTAATAAATCTTATAATTTATATCCTAGTTTTTTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                         <  2:30341/121‑1 (MQ=255)
                                        cATAAAATTAATAATATTTATAATTTTTATCATACGTATTTTTTTTTTTATTTTTTTTAATATAAAAATTAAAAAAAAAAAAACCCCATAAAAATTTAAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                         <  2:2149530/128‑1 (MQ=255)
                                             aaattAAAAATTTTTATAATTTATATACTCGTTATCTATTTTTTTATTTTTTTATCTTAACAAATAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                  <  2:687772/125‑1 (MQ=255)
                                              aattAAAAATTCTTATAATTTCTCACCCCCCGATCTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAACAAACCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                  <  2:1436751/126‑1 (MQ=255)
                                                    taatTATTATAATTTATAAACACGTTATCTATTTTTTTATTTTTTTATATTCAAAAATAAAAAAAAAAAAAACCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTa                                                                           <  2:3285272/128‑1 (MQ=255)
                                                     aatTTTTATAATTTATTTCCTCGTTAATTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTa                                                                           <  1:2504532/128‑1 (MQ=255)
                                                              aatttttataatCGTGTTATATTTTTTTTATTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCt                                                                  <  2:3250725/117‑1 (MQ=255)
                                                                  gttgagactagTGATTTATTTTTTTATTTGTTTAAATTCAAAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatta                                                              <  1:2554027/118‑1 (MQ=255)
                                                                    tttacTCGTTATATTTTTTTTTTTTTTTTTAAATTAAAAAATAAAAAAAAAAAAATACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                             <  2:1800133/124‑1 (MQ=255)
                                                                     aatacttttGATATATTTTTTTTATTTTTTAAATTAAAAAAAAAAAAAAAAAAAAACCCATAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                             <  2:1664829/120‑1 (MQ=255)
                                                                       acctgtttaattattttatttTTTTTTTTAATTAAAAAAAAAAAAAAAAAAAATCCAATT‑‑‑AAAATTTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                         <  2:1991824/117‑1 (MQ=255)
                                                                        tcttgttttatATTTTTTTTTTTTTTTTTATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                         <  1:289983/118‑1 (MQ=255)
                                                                        attcttttttttttttttttttttttTATTTTAAAAAAAAAAAAAAAAAAAAAACC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                         <  1:3073524/116‑1 (MQ=255)
                                                                         ctctttttatATTTTTTTTTTTTTTTATATTCAAAAATAAAAAAAAAAAAATACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                        <  2:1901750/119‑1 (MQ=255)
                                                                          acgttaaaaaTTTTTTTATTTTTTAAATTAAAAAAAAAAAAAAAAAAAAAACCAAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                     <  2:2451754/119‑1 (MQ=255)
                                                                           ttttttttatttttttattttttTATATTAAAAAAAAAAAAAAAAAAAAATACCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcaca                                                       <  2:776641/121‑1 (MQ=255)
                                                                           agtttaatATTTTTTTATATTTTTAAAATAAAAAAAAAAAAAAAAAAAATACCC‑‑‑TAAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                     <  2:940885/121‑1 (MQ=255)
                                                                            ttttttatttttttttttttttTATTTTAAAAATAAAAAAAAAAAAATTCC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                    <  1:324622/120‑1 (MQ=255)
                                                                            gtgttcattttttttatttttttaTATTAAAAAAAAAAAAAAAAACAAAACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                    <  1:518233/120‑1 (MQ=255)
                                                                            gtgaattatttattTAATTTTTTATATTAAAAAATAAAAAAAAAACAATCCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                    <  2:2220713/122‑1 (MQ=255)
                                                                             tatttattttttttattttttttatTAAAAAAAAAAAAAAAAAAAATACACTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                    <  2:1782113/121‑1 (MQ=255)
                                                                               tttttttttttttttttttTATTTTTAAAAAAAAAAAAAAAAAAAATCCCTTTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                                   <  1:2861835/123‑1 (MQ=255)
                                                                                  tatttttttattttttTATTATAACAAAAAAAAAAAAAAAAAAAACCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                                  <  2:1595783/128‑1 (MQ=255)
                                                                                  aaTTTTTTTAATTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                              <  2:34613/127‑1 (MQ=255)
                                                                                   ttttttttATTTTTTATATTTAAAAATAAAAAAAAAAAAATACCCTTT‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                             <  2:1471266/127‑1 (MQ=255)
                                                                                   aTTTTTTTATTTGTTTATTTTAAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                              <  1:2880068/128‑1 (MQ=255)
                                                                                    ttttttttttttttttTAATAAAAAAAAAAAAAAAAAAAATCCC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                             <  2:704445/128‑1 (MQ=255)
                                                                                    tttttttatttttttaTATTCACAAATAAAAAAAAAAAAATACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                             <  2:2525480/128‑1 (MQ=255)
                                                                                    tttttttatttttttaATTTAAAAAATAAAAAAAAAAAAATCACCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                            <  2:1267767/128‑1 (MQ=255)
                                                                                    tttttttAATTTTTTATTTTTAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                                         <  1:25313/128‑1 (MQ=255)
                                                                                    attttttttttttttATTTTATAAAAATAAAAAAAAAAAAAACCC‑‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                             <  1:343485/127‑1 (MQ=255)
                                                                                     ttttttttttttttttttTCAAAAAAAAAAAAAAAAAAAAAAACATTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                            <  1:754908/128‑1 (MQ=255)
                                                                                     ttttttATTTTTTTAATATAAAAAAAAAAAAAAAAAAAAT‑‑ACAATAAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                            <  2:1302584/128‑1 (MQ=255)
                                                                                     ttttttATTTTTTTAAATAAAAAAAAAAAAAAAAAAAATCCCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                            <  1:69777/128‑1 (MQ=255)
                                                                                      tttttATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAATAAAAAAAAAACATAAAAACAAAAAATATTTATTAAAAATACATATAATACAACatttttattaata                                           >  1:399265/1‑120 (MQ=255)
                                                                                       ttttttttGTTTATCTTAAAAAATAAAAAAAAAAAAAAACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                             <  2:1180043/128‑1 (MQ=255)
                                                                                       ttttATTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAATCCCATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                                         <  1:2743476/128‑1 (MQ=255)
                                                                                       ttttATTTTTTTTTATTAAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                          <  2:502882/128‑1 (MQ=255)
                                                                                        tttATTTTTTTTTTTTAAAAAATAAAAAAAAAAAAAAACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                          <  1:309779/128‑1 (MQ=255)
                                                                                        tttATTTTTTTATTATAAAAAAAAAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                           <  2:2328571/128‑1 (MQ=255)
                                                                                        tttATTTTTTTATTATAAAAAAAAAAAAAAAAAAAAAAAACTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                          <  2:3175445/128‑1 (MQ=255)
                                                                                         tttttttttTATCATAACAAATAAAAAAAAAAAAATACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAAt                                        <  2:2978128/125‑1 (MQ=255)
                                                                                           tttttttTATTTTAAAAAAAAAAAAAAAAAAAAAAACC‑‑TTAAAAATTATAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                      <  2:2033534/127‑1 (MQ=255)
                                                                                            ttttttTATATTAAAAAATAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                                     <  2:2860364/128‑1 (MQ=255)
                                                                                               gTTTATATTAAAAAAAAAAAAAAAAAAAAATCCCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                                    <  2:1715456/128‑1 (MQ=255)
                                                                                                 ttaaaattaaaaaaaaaaaaaaaaaaaaaACCCTT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                                <  2:3153198/117‑1 (MQ=255)
                                                                                                 ttATCTTCACAAATAAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAATAAAAAAAACATTATTTTTTAAAATTGACTATTTTAAAAAATTTTTTTTTTAAAAAAATATAtt                                >  2:2935366/1‑128 (MQ=255)
                                                                                                    ataataaaaaaaaaaaaaaaaaaaATCCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                              <  2:227071/120‑1 (MQ=255)
                                                                                                      atataaaaaaaaaaaaaaaaaaaaaaCCCATAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                              <  1:1397449/122‑1 (MQ=255)
                                                                                                       ttaaaaaaTAAAAAAAAAAAAATCCACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                          <  2:585775/123‑1 (MQ=255)
                                                                                                        taaaaaaaaaaaaaaaaaATAAACCCTTTAAAAATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                            <  2:1054519/124‑1 (MQ=255)
                                                                                                             aaTAAAAAAAAAACTATAACATTT‑‑AAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatata                     <  2:2360018/128‑1 (MQ=255)
                                                                                                                   aataaaaaaaaACTCCTCTTTACAATTTAAAATAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTGTTAAAATAAGATATTGACATAatatatat                  <  2:650074/125‑1 (MQ=255)
                                                                                                                    aaaaaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACAAAAAACAAACAAAATTTAAAAAAATAACATTTTAAAAAAATTTTAATTTTTAAATAAAAATATTTATATAatatataaata               >  1:2181414/1‑124 (MQ=255)
                                                                                                                                   |                                                                                                                                
AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATAC  >  CP016816/202358‑202612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: