Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4860T.55.1% 219.8 / inf 298intergenic (+264/‑411)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (13/101);  new base . (62/78);  total (96/202)
Fisher's exact test for biased strand distribution p-value = 7.12e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-10

Only 100 of 326 total aligned reads displayed.

AGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCCTTTTAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACT  >  CP016816/202359‑202613
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atatAGTGTAAAAAATATTTTTAAGGTTAATTAAAAAACCATAAAATTAAAAATTCT‑TAAAAAGTAGAACCACGTGATAAATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCCtt                                                                                                                                   <  2:848162/125‑1 (MQ=255)
aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCCtt                                                                                                                                   <  2:2117023/128‑1 (MQ=255)
  ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCTTGATCTATTTTTTAATATGTATAT‑CAT‑CACAAAT‑AAAAAAAAACAATCACCttaaa                                                                                                                                >  1:2757621/1‑125 (MQ=255)
  ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATACCCttaa                                                                                                                                 >  1:3142886/1‑126 (MQ=255)
  ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTATATTAATATAT‑CTT‑CACAAATAAAAAAAAAACAAACCCAttaa                                                                                                                                 >  1:292350/1‑126 (MQ=255)
          aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCCttaaaaataaaa                                                                                                                         >  1:2978128/1‑118 (MQ=255)
            tattaGAGTTAAGGTTTATTAAAATACCATAAACTTAATTATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAATAAACAATCAC‑‑Attaaa‑a‑attaaaaa                                                                                                                   >  1:2618536/4‑128 (MQ=255)
            taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTAT‑CTT‑CACAAAAAAAAAAAAAACAATCACC‑‑ttaaa‑a‑attaaaaa                                                                                                                   >  2:2870174/1‑128 (MQ=255)
             aataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAAACCAC‑‑Ttaaa‑a‑attaaaaaa                                                                                                                  >  2:2620495/1‑128 (MQ=255)
                    ttAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CAAAAATAAAAAAAAAACAATCCC‑‑‑ATAAA‑A‑AATaaaaaaaaaaaaaa                                                                                                          >  1:1054519/1‑128 (MQ=255)
                     tAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTATATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAACCAATCCCCTT‑‑‑AA‑A‑AATaaaaaaaaaaaaaaa                                                                                                         >  1:1764357/1‑128 (MQ=255)
                     tAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaa                                                                                                          >  1:925036/1‑128 (MQ=255)
                     tAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTATATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCAAC‑‑TTAAA‑A‑AATaaaaaaaaaaaaaa                                                                                                          >  1:1179594/1‑128 (MQ=255)
                     tAAGGTTAATTAAAATACCATAAACTTAATAATTAT‑TATAATGTAGATACTCTTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAAAAAAC‑‑‑TAAA‑A‑AATtaaaaaaaaaaaaaa                                                                                                         >  2:754908/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCC‑‑‑TAAA‑A‑AATaaaaaaaaaaaaaaaa                                                                                                        >  1:3032958/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCAC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaa                                                                                                         >  1:2809921/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CAAAAATAAAAAAAAAAAAAATCACATaaaaa‑a‑aaaaaaaaaaaaaaaa                                                                                                           >  1:1889445/1‑128 (MQ=255)
                       aGGTTAATTAAAATACCATAAACTTAATAATTCA‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑AACAAATAAAAAAAAAACAAT‑‑CAAATTAAA‑A‑ATTaaaaaaaaaaaaaaaa                                                                                                        >  2:1924852/1‑128 (MQ=255)
                        ttttatttatAATAAAATAAAATTAATAATAAT‑TTTAATTTTTATTATCCTGTTTTATTTTTTTATTTTTTTAT‑TTT‑AAAAAATAAAAAAAAAAAAAACCCC‑‑‑TAAA‑A‑AATtaaaaaaaaaaaaaaaaa                                                                                                      <  2:2951828/126‑1 (MQ=255)
                        ggTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaa                                                                                                       >  2:2821333/1‑128 (MQ=255)
                        ggTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCACGAGATCTATTTTTTTATATCTTTAT‑CTT‑CACAAAAAAAAAAAAAACAATCCACaaaaaaa‑a‑aaaaaaaaaaaaaaaaaa                                                                                                         >  1:3101567/1‑128 (MQ=255)
                        ggTTAATTAAAATAACAAAAAATTAAAAATTTT‑TATAAAGTATATCCTCGGTATATATTTTTTTATTTGTTTAA‑TTT‑AACAAA‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaaa                                                                                                      <  1:2842369/128‑1 (MQ=255)
                         tttaattaaAATAAAATAAAATTAAAAAATAT‑TATAATTTTTATGCACTTTTTATTTTTTTTTTTTTTTTTTT‑TTT‑AAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAA‑A‑AATaaaaaaaaaaaaaaaaaa                                                                                                      <  1:2870174/127‑1 (MQ=255)
                         tttaattaaAAAACCAAAAAAATAAAAATTTT‑TTTAATTTATATACTTTTTATCTTTTTTTTTTTTTTTTTTA‑TTT‑AAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaaa                                                                                                      <  1:3299482/127‑1 (MQ=255)
                          ttaatttaAAAAACATAAAATTAAAAATTAT‑TATAATTTATATCCTCTTTAATTTTTTTTTTATTTTTTTAT‑ATTAAAAAAAAAAAAAAAAAACAAACCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaaa                                                                                                      <  2:241237/128‑1 (MQ=255)
                          ttaattaaAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTATTATTTGTTTAT‑ATT‑CACAAATAAAAAAAAAACAAAACC‑‑‑ATAAA‑A‑AATtaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:3253919/1‑128 (MQ=255)
                          ttaattaaAATAAAATAAAATTAAAAAATAT‑AAAAAATTATAAACTAGTTATATATTTTTTTATTTTTTTAT‑ATT‑AAAAAAAAAAAAAAAAACAATCCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaaaa                                                                                                     <  2:1503210/128‑1 (MQ=255)
                          ttaattaaAAAAACATAAAATTAAAAATTCTATAAAAT‑TATAAAATCCTTATCTATTTTTTTATTTTTTTAT‑ATT‑AAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaaaa                                                                                                     <  2:381143/128‑1 (MQ=255)
                          gttattaaAAAAACATTAAATTAATAAATAA‑TATAATTTATAACCTATTGAAATATTTTTTTATTTTTTTAT‑CAT‑CAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAA‑A‑AATaaaaaaaaaaaaaaaaaaa                                                                                                     <  2:1462272/125‑1 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCTTGATCTAATTTATAATTTTAATAT‑ATA‑CACAAAAAAAAAAAAAACAAACCAC‑‑ATAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaa                                                                                                    >  1:27357/1‑128 (MQ=255)
                            aattaaAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAAAAATAAC‑‑ATAAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaaa                                                                                                   >  1:1504950/1‑128 (MQ=255)
                            aattaaAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAA‑AAAATCAACTTAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:2572127/1‑128 (MQ=255)
                            aattaaAATAACAAAAAATTAATAATTTT‑TATAATTTTTATACTCTTTTTGTATTTTTTTATTTTTTTAT‑CTT‑AAAAAATAAAAAAAAAACAATCCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaaa                                                                                                   <  2:3036418/128‑1 (MQ=255)
                             attaaAATACCATAAAATTAATAAATCT‑TATAATGTAAATACTAATTATATATTTTAATATTAGTTTAT‑ATA‑CACAAATAAAAAAAAAAAAAT‑‑CAAATAAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  2:2041781/1‑127 (MQ=255)
                              ttaaAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATACCC‑‑TAAAA‑A‑AATaaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  1:709381/1‑126 (MQ=255)
                              ttaaAATACCATAAACTTAATAATTCT‑TATAATCTAGATCATCGAGATCTATATTTTTATTTTTTTAT‑CTT‑CAAAAATAAAAAAAAAACAA‑‑ACAAATAAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  2:2723145/1‑126 (MQ=255)
                                aaaaataaaaaaaaTTAATAATTAT‑TATAATTTATATAAAATTTATAAATTTTTTTATTTTTTTAA‑CAT‑AAAAAAAAAAAAAAAAACAAACCCCTT‑‑‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTaaa                                                                                              <  2:961785/118‑1 (MQ=255)
                                aaaaTACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                               >  2:1746793/1‑124 (MQ=255)
                                aaaaTACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATACCC‑‑TTAAA‑A‑AATaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                               >  1:673658/1‑124 (MQ=255)
                                aaaaTACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAAACCACATAAAAA‑T‑aaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  1:783334/1‑126 (MQ=255)
                                aaaaTAAAAAAAAATTAATAATTTT‑TATAATTTTTATCCTCGTTTTTTTTTTTTTTTTTTTTTTAT‑ATT‑AAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaaaacta                                                                                               <  1:3235256/128‑5 (MQ=255)
                                 aaaTACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCC‑‑TAAAA‑A‑ATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  1:350230/1‑123 (MQ=255)
                                  aataaaaTAAAATTAATAATTTT‑TATAATTTTTATAATAGTGTTATTTTTTTTTATTTGTTTAT‑CTT‑TAAAAAAAAAAAAAAAAAAATCCCCTTAAAAA‑T‑TAAAAAAAAAAAAAAAAAAAAAAACTaa                                                                                               <  1:2565354/122‑1 (MQ=255)
                                  aaTACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCC‑‑TTAAA‑A‑ATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                             >  2:393669/1‑128 (MQ=255)
                                  aaTACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCACC‑‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAA‑Taaaaa                                                                                            >  1:1747534/1‑128 (MQ=255)
                                       caaaaaattaaaaattaT‑TAAAAATTATATACTCGTGTTCTATTTTTTTATTTGTTTAT‑ATT‑AAAAAATAAAAAAAAAAAAAACCCCTT‑‑‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAaaacaaac                                                                                       <  2:2697627/125‑1 (MQ=255)
                                          aaaataaataaataT‑TATAATATAGATCATTGTATACTAATTTTTTAATTTTTTAT‑CAA‑AAAAAAAAAAAAAAAAAAAATACCCAT‑‑‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                                    <  2:1096664/122‑1 (MQ=255)
                                           aaataaataatTAT‑TATAATTTATATACACGTTATCTATTTTTTTTTTTTTTTAT‑CTT‑AAAAAATAAAAAAAAAACAATACCC‑‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                                    <  2:2600248/123‑1 (MQ=255)
                                            aattAATAATTTT‑TATAATTGATTTCCCATTGATCTTTTTTTTTATTTTTTTAT‑GTT‑CAAAAAAAAAAAAAAAACAATCCCC‑‑TTAAA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                   <  1:2506017/126‑1 (MQ=255)
                                             ataaataatTAT‑TAAAATTTACATACACTTTATAAATATTTTTATTTTTTTAT‑AAT‑AAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AA‑A‑AAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAttt                                                                                <  2:520378/125‑1 (MQ=255)
                                              ttAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAAT‑AAAAAAAAACAATCCCC‑TTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAAATAAaaacaaaataattt                                                                                 >  1:2892987/1‑121 (MQ=255)
                                                  taatTCT‑TATAATTTAGATTCACTTGATATATTTTTTAATTTGTTAAT‑ATT‑CAAAAAAAAAAAAAAAAAAATCCCC‑‑TAAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCtt                                                                             <  2:307447/128‑1 (MQ=255)
                                                                   tttacTCGTTATATTTTTTTTTTTTTTTTTAA‑ATT‑AAAAAATAAAAAAAAAAAAATACCC‑TTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                              <  2:1800133/124‑1 (MQ=255)
                                                                   taaccTCGTTTTTTATTTTTTTATTTTTTTAT‑TAT‑AAAAAAAAAAAAAAAAAAAAACCCC‑‑ATAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATtata                                                             <  1:3077460/125‑1 (MQ=255)
                                                                    atcaactttttttatttttttattttttTAT‑CTT‑CAAAAATAAAAAAAAAACAATCCCC‑TTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATtata                                                             <  2:3169814/116‑1 (MQ=255)
                                                                     tactcttgttatatttttttattttttTAT‑ATT‑AACAAAAAAAAAAAAAAAAAAAACC‑TTAAAA‑A‑ATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                            <  1:3193475/117‑1 (MQ=255)
                                                                     tCCTCTTTATATATTTTTTTATTTGTTAAG‑ATT‑‑AAAAAAAAAAAAAAAACAATCCCC‑‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTGAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                          <  2:1664044/128‑1 (MQ=255)
                                                                     acctgtttaattattttatttTTTTTTTTA‑ATT‑AAAAAAAAAAAAAAAAAAAATCCAATT‑‑‑AA‑A‑ATTTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                          <  2:1991824/117‑1 (MQ=255)
                                                                      actcctttgttttttttctATTTGGTTTT‑ATT‑TAAAAAAACAAAAAAAAAAATCGC‑‑TTTAAA‑A‑ATTCAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                          <  2:2451549/116‑1 (MQ=255)
                                                                      actatttttatatttttttattttttTAT‑ATT‑AAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                          <  2:468790/118‑1 (MQ=255)
                                                                       ctctttttatATTTTTTTTTTTTTTTAT‑ATT‑CAAAAATAAAAAAAAAAAAATACCC‑‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                         <  2:1901750/119‑1 (MQ=255)
                                                                       attgtgtttttttttttttttttttTAT‑ATT‑‑AAAAATAAAAAAAAAAAAAAATCCCATT‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                         <  1:401517/117‑1 (MQ=255)
                                                                        ttttttttatttttttttttttttTAA‑TTT‑AAAAAATAAAAAAAAAAAAAATCACTTAAAAA‑T‑TAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                          <  2:69318/118‑1 (MQ=255)
                                                                         agtttaatATTTTTTTATATTTTTAA‑AAT‑AAAAAAAAAAAAAAAAAAAATACCC‑‑‑TAAA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                      <  2:940885/121‑1 (MQ=255)
                                                                          tttttttatttttttattttttTAT‑TTT‑TAAAAATAAAAAAAAAAAAATTCC‑‑TAT‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                     <  1:2643021/122‑1 (MQ=255)
                                                                           tatttattttttttattt‑ttttt‑atT‑AAAAAAAAAAAAAAAAAAAATACAC‑TTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                     <  2:1782113/121‑1 (MQ=255)
                                                                             aaatatttttttattttttTTA‑ATT‑AAAAAAAAAAAAAAAAAAAATAACAATAAAAATT‑AAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                      <  2:1272237/125‑1 (MQ=255)
                                                                              tttATTTTTTTTTTTTTTTATGATT‑AAAAAAAAAAAAAAAAAATATCCCCTAT‑‑AA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                                   <  2:1389264/126‑1 (MQ=255)
                                                                                ttttttttttttttttTAA‑TAT‑AAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AA‑A‑AATTTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                               <  2:1121472/126‑1 (MQ=255)
                                                                                tatttttttattttttTAT‑TAT‑AACAAAAAAAAAAAAAAAAAAAACCTTAAAAATT‑AAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                                   <  2:1595783/128‑1 (MQ=255)
                                                                                 atttttttatttttttAT‑TTT‑AAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AA‑A‑AAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                             <  1:2460813/128‑1 (MQ=255)
                                                                                  tttttttatttttttaT‑ATT‑CACAAATAAAAAAAAAAAAATACCC‑‑TTAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                              <  2:2525480/128‑1 (MQ=255)
                                                                                  tttttttATTT‑TTTATAATT‑AAAAAATAAAAAAAAAAAAAAAACCTT‑‑‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                             <  2:2782351/128‑1 (MQ=255)
                                                                                   ttttttttttttttAT‑‑TT‑TAAAAATAAAAAAAAAAAAAACACATTAAAAA‑ATAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                               <  2:551325/128‑1 (MQ=255)
                                                                                    ttttttttttttttT‑ATT‑AAAAAAAAAAAAAAAAAAAAAACCCTTT‑‑AA‑A‑AAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                           <  2:2911247/128‑1 (MQ=255)
                                                                                    ttttttttttttTAT‑ATT‑AAAAAAAAAAAAAAAAACAATCGCC‑‑TTAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                            <  1:2723145/128‑1 (MQ=255)
                                                                                     ttttttttGTTTAT‑CTT‑AAAAAATAAAAAAAAAAAAAAACCCTTAAAAATT‑AAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                              <  2:1180043/128‑1 (MQ=255)
                                                                                     ttttAATTTTTTAT‑ATA‑TAAAAAAAAAAAAAAAAAAAAAACCAA‑TAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                            <  2:1439331/128‑1 (MQ=255)
                                                                                      tttATTTTTTTAT‑TAT‑AAAAAAAAAAAAAAAAAAAATCCCCTTAAAAA‑T‑TAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                            <  2:2328571/128‑1 (MQ=255)
                                                                                        tATTTTTTTAT‑ATT‑AAAAAATAAAAAAAAAACAATCCAATT‑‑‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                       <  2:597370/128‑1 (MQ=255)
                                                                                             ttttaaaatt‑CAAAAATAAAAAAAAAAAAAAACCC‑‑TTAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGat                                    <  2:785214/120‑1 (MQ=255)
                                                                                             gTTTAT‑ATT‑AAAAAAAAAAAAAAAAAAAAATCCCCTTAAAA‑A‑ATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                                     <  2:1715456/128‑1 (MQ=255)
                                                                                               ttAT‑TTT‑TAAAAAAATAAAAAAAAAAATTACCATTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                                   <  2:43690/128‑1 (MQ=255)
                                                                                                  tatttttaaaaataaaaaaataaaaATACACTTT‑‑AA‑A‑AAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAAATCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                               <  1:1953005/119‑1 (MQ=255)
                                                                                                  taaaattaaaaaaaaaaaaaaaaaaATACCCTT‑‑‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                               <  2:3021972/119‑1 (MQ=255)
                                                                                                   attttaacaAATAAAAAAAAAAAAATACCC‑‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                               <  2:2196048/122‑1 (MQ=255)
                                                                                                   ataataaaaaaaaaaaaaaaaaaaATCCCC‑‑TTAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                               <  2:227071/120‑1 (MQ=255)
                                                                                                     attaaaaaaaaaaaaaaaaaaaaaCACCTTT‑‑AA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                             <  2:67031/122‑1 (MQ=255)
                                                                                                     atataaaaaaaaaaaaaaaaaaaaaaCCCATAAAA‑A‑ATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                               <  1:1397449/122‑1 (MQ=255)
                                                                                                      ttaaaaaaaaaaaaaaaaaaaaaACCATTTAAAA‑A‑TAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGAc                              <  1:730960/123‑1 (MQ=255)
                                                                                                       tacaaaaaaaaaaaaaaaaaATACCAATAAAAA‑ATAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGAc                              <  1:2170782/124‑1 (MQ=255)
                                                                                                         caaaaaaaaaaaaaaaCAA‑‑ACCAATTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataat                         <  2:3227550/126‑1 (MQ=255)
                                                                                                            aaTAAAAAAAAAACTATAACATTT‑‑AA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatata                      <  2:2360018/128‑1 (MQ=255)
                                                                                                             attaaaaaaaaaCAATCCCATT‑‑‑AA‑A‑AAT‑ATAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatatat                   <  1:2492272/125‑1 (MQ=255)
                                                                                                               aaaaaaaaaaaaATCCCCTT‑‑‑AA‑A‑AATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATc                  <  2:1878412/128‑1 (MQ=255)
                                                                                                                 aaaaaaaaaaATCCCC‑TTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATc                  <  2:344983/128‑1 (MQ=255)
                                                                                                                   aaaaaaaaATACACTTTTAAA‑ATAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATc                  <  1:2536975/128‑1 (MQ=255)
                                                                                                                      aaaCAATCCCC‑TTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAACAAAAAACAAACAATATTTATTAAAATACCATTTTATACCAAATTTTATTTTTAAAAAAAGATATTAACATAatatatataaaatt             >  2:2746662/1‑122 (MQ=255)
                                                                                                                      aaaCAATCCCC‑‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAA‑AAAAAACAAACACAATTAATTAAAATTCCATATTATACCACATTTTATTTTTAAAAAAAAAAATTGACAAAATtaatatataatata           >  2:3185941/1‑128 (MQ=255)
                                                                                                                                  tttAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACt  <  1:2937134/128‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
AGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCT‑TATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTAT‑CTT‑CACAAATAAAAAAAAAACAATCCCCTTTTAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACT  >  CP016816/202359‑202613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑