Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 478,738 C→A G301V (GGT→GTT)  JCVSYN2_02210 ← DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,7380CA99.3% 3901.9 / NA 1335G301V (GGT→GTT) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (5/4);  new base A (657/663);  total (662/673)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1367 total aligned reads displayed.

GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAA  >  CP016816/478613‑478862
                                                                                                                             |                                                                                                                            
  aaCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacacc                                                                                                                          >  1:3122199/1‑128 (MQ=255)
  aaCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacacc                                                                                                                          >  2:1035982/1‑128 (MQ=255)
    cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                        >  2:836974/1‑128 (MQ=255)
     aTACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATc                                                                                                                       >  1:585455/1‑128 (MQ=255)
      tACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCt                                                                                                                      >  2:801258/1‑128 (MQ=255)
       aCGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTa                                                                                                                     >  1:699733/1‑128 (MQ=255)
        cGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTaa                                                                                                                    >  2:3286901/1‑128 (MQ=255)
        cGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTaa                                                                                                                    >  2:744229/1‑128 (MQ=255)
          tttttCATTATTTACAATTATTGATGGTGGACACATTTCTTTAAATTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAtt                                                                                                                  <  1:2128539/128‑1 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                             >  1:129722/1‑128 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                             >  2:1447144/1‑128 (MQ=255)
                attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                            <  1:3049594/128‑1 (MQ=255)
                       aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                     >  1:1968125/1‑128 (MQ=255)
                        cAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGa                                                                                                    >  1:372006/1‑128 (MQ=255)
                            aatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                                                                                <  2:545518/128‑1 (MQ=255)
                            aatTGATCTTTCAACCATTTCTATAACTTTTTTTTATATATCATTTCTAATAATGATTCTTCTATATAAATCATTATTTTCAGAAGTAGTAAATCTAACAACATCTAATTGAATAATTGGACGAATGt                                                                                                <  1:488981/128‑1 (MQ=255)
                              ttGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                                                                              <  2:3128157/128‑1 (MQ=255)
                              ttGATGGTGCAACCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                                                                              <  2:372578/128‑1 (MQ=255)
                               tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtg                                                                                             >  2:2035977/1‑128 (MQ=255)
                               tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtg                                                                                             <  1:2880536/128‑1 (MQ=255)
                                gATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtgg                                                                                            >  1:1910006/1‑128 (MQ=255)
                                 aTGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATTTAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggt                                                                                           >  1:1264876/1‑128 (MQ=255)
                                  tGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtg                                                                                          >  2:2240430/1‑128 (MQ=255)
                                  tGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAAAAATTGGACGAATGTAtggtg                                                                                          >  1:142108/1‑128 (MQ=255)
                                    gTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                                                                        <  2:195508/128‑1 (MQ=255)
                                    gTGCACCCATTTCTTTAACTTTTTTTAATCTATCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                                                                        <  2:1227829/128‑1 (MQ=255)
                                      gacctaaTTTCTTAAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAATTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAAt                                                                                      <  1:2267390/122‑1 (MQ=255)
                                      gCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAAt                                                                                      >  1:882759/1‑128 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     <  2:1162693/128‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     <  2:2500461/128‑1 (MQ=255)
                                        aCCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATaa                                                                                    <  1:100461/128‑1 (MQ=255)
                                         cccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAAc                                                                                   <  1:2818320/128‑1 (MQ=255)
                                           cATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTg                                                                                 <  2:2615623/128‑1 (MQ=255)
                                           cATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTg                                                                                 >  2:886831/1‑128 (MQ=255)
                                              ttCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGaa                                                                              >  2:1817260/1‑128 (MQ=255)
                                               tCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAt                                                                             <  1:3294581/128‑1 (MQ=255)
                                                atttAAATTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAtt                                                                            <  1:2322014/127‑1 (MQ=255)
                                                   tAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTGAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAct                                                                         >  1:1494245/1‑128 (MQ=255)
                                                   tAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAct                                                                         <  1:3242817/128‑1 (MQ=255)
                                                      cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                      >  1:2704623/1‑128 (MQ=255)
                                                      cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                      <  1:2994672/128‑1 (MQ=255)
                                                        ttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCAt                                                                    >  2:2864099/1‑128 (MQ=255)
                                                        ttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCAt                                                                    >  1:24133/1‑128 (MQ=255)
                                                           tttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAAc                                                                 >  2:1663796/1‑128 (MQ=255)
                                                             tAATCTTTCATTTATAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACca                                                               <  2:1312177/128‑1 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATg                                                                                                 >  2:610268/1‑92 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                             >  1:636064/1‑128 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATGATTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                             <  1:1782205/128‑1 (MQ=255)
                                                                   ttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcatt                                                         >  2:3056909/1‑128 (MQ=255)
                                                                   atCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcagtcatt                                                         >  1:256697/2‑128 (MQ=255)
                                                                    tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtgg                                                                                         >  1:1850889/1‑95 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACTAATGTCTGGTGGAATAACTGGAATAACTCTCATAACCAttcattca                                                       >  2:1093189/1‑128 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGTATAACTGGAATTACTCTCATAACCAttcattca                                                       >  2:1537551/1‑128 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                       >  2:2167613/1‑128 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                       >  2:2664219/1‑128 (MQ=255)
                                                                      aTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAg                                                      <  1:3185269/128‑1 (MQ=255)
                                                                         tCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGAc                                                   <  1:2038798/128‑1 (MQ=255)
                                                                          cTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACg                                                  >  2:45479/1‑128 (MQ=255)
                                                                                atGATTCTTCTATATTAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGATGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            <  2:2296553/128‑1 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            <  2:1581614/128‑1 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            >  2:2006830/1‑128 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            >  1:750672/1‑128 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                             >  1:1558756/1‑127 (MQ=255)
                                                                                 tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACCCCATCTAATTGAAGAATTGTACGAATGTCTGGTGGATTAACTGTAATTCCTCTCATAACCCTTCATTCAGGACggtggtga                                           >  1:985115/1‑126 (MQ=255)
                                                                                 tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTa                                           <  1:1083264/128‑1 (MQ=255)
                                                                                     tcttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTTGAATAACTGGAATAACTCTCATAACCATTCATTCAGGACGTTCGTTAGAAc                                       >  2:2794114/1‑128 (MQ=255)
                                                                                     tcttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAAc                                       >  1:2622712/1‑128 (MQ=255)
                                                                                        tctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtt                                    <  2:1202812/128‑1 (MQ=255)
                                                                                        tctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGAGGAATATCTGGAATTACTCTCATAACCAATCATTCAGGACGTTGGTTAGAAAGtt                                    >  2:946783/1‑128 (MQ=255)
                                                                                         ctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGttt                                   <  2:2556863/128‑1 (MQ=255)
                                                                                          tatataAATCATTAATTTCAGAATTAGTACATCTAACACCATCTAATTGAATAATTGGACGAATGTCGGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtttt                                  <  1:2187782/128‑1 (MQ=255)
                                                                                          tatataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCACGACCTGGGTTAGAACGtttt                                  >  1:1701119/1‑128 (MQ=255)
                                                                                             ataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaaa                               <  2:682315/128‑1 (MQ=255)
                                                                                              tacaTCATTAATTTCAGAAGTTGTAAATCTAACACCAGCTAATTGAATAATTGTACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAg                              <  1:432218/125‑1 (MQ=255)
                                                                                                     ttAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCATGACGTTGGTTAGAACGTTTTTAAGCATCaaa                       >  2:2101235/1‑128 (MQ=255)
                                                                                                     ttAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaga                       <  2:2733027/128‑3 (MQ=255)
                                                                                                         tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGCACGTTTTAAAGCATCAAAAGtt                   >  1:1203669/1‑128 (MQ=255)
                                                                                                         tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaacta                   >  1:1350194/1‑125 (MQ=255)
                                                                                                         tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGtt                   <  2:3212082/128‑1 (MQ=255)
                                                                                                         tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGtt                   <  2:1272889/128‑1 (MQ=255)
                                                                                                          ttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGttt                  >  2:1873743/1‑128 (MQ=255)
                                                                                                          ttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAATGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGttt                  <  1:1610547/128‑1 (MQ=255)
                                                                                                           tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTc                 >  2:2989070/1‑128 (MQ=255)
                                                                                                           tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGATTAACTGGAATTACTATCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTc                 >  1:2471799/1‑128 (MQ=255)
                                                                                                           tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTc                 <  1:2935032/128‑1 (MQ=255)
                                                                                                             aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACGAATTTCTGCTGGAATAACTGGAATTACTCTAATAACCAATCCTTCAGGACGTTGGTTAGAACGTTTTAAAGAATCAAAAATTTCTa               >  2:418986/1‑128 (MQ=255)
                                                                                                              gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTaa              <  1:2736083/128‑1 (MQ=255)
                                                                                                              gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTaa              <  1:2520965/128‑1 (MQ=255)
                                                                                                                  tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGt          >  2:17437/1‑128 (MQ=255)
                                                                                                                   agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtt         >  1:929163/1‑128 (MQ=255)
                                                                                                                    gtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGttt        >  2:2447896/1‑128 (MQ=255)
                                                                                                                      aaaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTta      <  1:836654/128‑1 (MQ=255)
                                                                                                                       aaTCTAACCCCATCTAATTGAATAATTTGACGAATGTCTGGTGGAAGAACTGGAATTACTCGCATAACCATTCATTAAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTGCTGAActgtatta     >  2:1642776/1‑121 (MQ=255)
                                                                                                                       aaTCTAACACCTTCTAATTGAATAATTGGACGAATGACTGGTGGAATAACTTGAAATACTCTAATAACCAGTCATTAATTACATTGTTTTGAACTTTTTTAATCATATAAAGTTTCTATAAGTTTtaa     >  1:1653714/1‑128 (MQ=255)
                                                                                                                       aaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTGTCTAAACGTTGtaa     >  2:3179338/1‑128 (MQ=255)
                                                                                                                        aTCTACCCCCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGATCGTTTTAATGCATCAAAAGTTTCTAAACGTTTttat    >  2:1383032/1‑128 (MQ=255)
                                                                                                                         tCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaata   <  2:148847/128‑1 (MQ=255)
                                                                                                                         tCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaata   >  1:1157992/1‑128 (MQ=255)
                                                                                                                          cTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaataa  >  2:2927215/1‑128 (MQ=255)
                                                                                                                          cTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaataa  >  2:2354025/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAA  >  CP016816/478613‑478862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: