BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,222	G→A	intergenic (+186/‑356)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,222	0	G	A	98.7%	3799.7 / 30.6	1250	intergenic (+186/‑356)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (803/428); minor base . (5/11); total (808/442)
Fisher's exact test for biased strand distribution p-value = 7.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1271 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165097‑165347

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               >  2:1231204/1‑128 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               <  2:944741/128‑1 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               >  2:386208/1‑128 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               >  2:2447131/1‑128 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                             >  2:3171939/1‑128 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                             <  2:3041098/128‑1 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGATTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                             <  2:1380980/128‑1 (MQ=255)

     aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                           >  1:109099/1‑128 (MQ=255)

      ttGTATTACTTTACTAGTTGATAATA‑TTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  2:2193952/128‑1 (MQ=255)

       tGTATTACTTTTCTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:316425/128‑1 (MQ=255)

       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  2:2517369/128‑1 (MQ=255)

       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  2:2870779/128‑1 (MQ=255)

          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAAATATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      >  1:1412637/1‑128 (MQ=255)

             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                   >  1:236579/1‑128 (MQ=255)

               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:1767457/1‑128 (MQ=255)

               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:8232/1‑128 (MQ=255)

               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:2846948/1‑128 (MQ=255)

                 tactCGTTGATAATATTTAGTTTTATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTTATTTGAACTTGTaa                                                                                                               <  1:534616/128‑1 (MQ=255)

                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             >  2:2571273/1‑128 (MQ=255)

                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             >  1:987891/1‑128 (MQ=255)

                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATATTTAAAAAATTAATTTTAACTTGTaaaa                                                                                                             >  1:2573153/1‑128 (MQ=255)

                   ctAGTTGATAATATTAAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             <  1:1008904/128‑1 (MQ=255)

                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         >  1:1489588/1‑107 (MQ=255)

                                tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt‑ttttt                                                                                              >  2:2365117/1‑128 (MQ=255)

                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt‑ttttttt                                                                                            >  1:1141201/1‑128 (MQ=255)

                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt‑ttttttt                                                                                            >  1:580885/1‑128 (MQ=255)

                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt‑ttttttt                                                                                            >  1:2838572/1‑128 (MQ=255)

                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGA‑tt‑ttttttttt                                                                                          >  2:775775/1‑128 (MQ=255)

                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGA‑tt‑ttttttttt                                                                                          >  2:47170/1‑128 (MQ=255)

                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTATTAAAAAATTAATTAGAACTTGTAAAAAATATC‑tt‑tttttatttt                                                                                         >  2:3326829/1‑128 (MQ=255)

                                       tCATTAATTTATCGCACTTGTATGTATTAGTAAGAAACTAAATCTATTATCTTTTTAGATATTTTTTGTATATGCAAATATATAAAAAATTAAATTGAACTTATAAAAAATATC‑tt‑tttttttatttt                                                                                       >  2:1109453/1‑128 (MQ=255)

                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt‑ttttttttttttt                                                                                      >  1:1753482/1‑128 (MQ=255)

                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt‑ttttttttttttttt‑tata                                                                               >  2:700802/1‑125 (MQ=255)

                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt‑tttttttttttttataaat                                                                                >  1:1931576/1‑122 (MQ=255)

                                                tATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATTGC‑TT‑TTTTTTTTTTTTTTt‑ataatt                                                                             >  1:1695567/1‑125 (MQ=255)

                                                 ttagCATTTGTTTGAATTATTTAGAAAATAAAAATTTTAACTTTTTATTTTTTTTTTGTTTTTGGAAATTTTTTAAAAATTTATTTGTACTTGTAAAAAATAGC‑‑G‑TTTTTTTTTTTTTTT‑ATATAATg                                                                           <  1:1268742/125‑1 (MQ=255)

                                                 aTCGCACTTGTATGAATTAGTAAGAAACTAATACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGA‑TTATTTTTTTTTTTTTTT‑Ttattt                                                                             >  1:245862/1‑126 (MQ=255)

                                                  tagtatttttATGAATTAATAATACAATAAAAATATTATATTTTTTTTTATATTTTTTTAATTAAAATTTTTAAAAAATTTATTTGAACTTTTAAAAAAAAAC‑‑‑‑TTTTTTTTTTTTTTT‑TTATAATGGa                                                                         <  2:2679018/119‑1 (MQ=255)

                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTATTTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑TTATATTga                                                                          >  2:1655426/1‑127 (MQ=255)

                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTAT‑TTATAAttaa                                                                         >  2:1044162/1‑125 (MQ=255)

                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TA‑TTTTTTTTTTTTTTt‑ataattgtaaata                                                                      >  2:2934490/1‑118 (MQ=255)

                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt‑ttttttttttttttt‑tattttttaaata                                                                      >  1:2707227/1‑116 (MQ=255)

                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATTTAAata                                                                     >  2:3272397/1‑128 (MQ=255)

                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATTAAAata                                                                     >  2:2155834/1‑128 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑TTATAAtaaaaaaa                                                                     >  2:1328567/1‑121 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTT‑TTATAATTTAAAtata                                                                   >  2:1600313/1‑128 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAAAATTATTTTAAAATTTTAAAAAATAGA‑TT‑TTTTTTTTTTTTTTT‑atatttaaatataa                                                                     >  1:359139/1‑118 (MQ=255)

                                                           tATGAATTAGTAAGAAACTAAAACTATAATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGA‑TT‑TTTTTTTTTTTATTT‑TAAAAAATAAAATATaa                                                                  >  1:72115/1‑128 (MQ=255)

                                                            aagatattgtcaGAAAATTAAAATATTATATGTTTTGATATTTTTTGTATAAGCATATATTTAAAAAATTAATTTGTACTTTGAAAAAATA‑‑‑TC‑GTTTTTTTTTTTTTT‑TTATAATGGAAATATAAAtt                                                               <  1:3017860/117‑1 (MQ=255)

                                                              tatttattaATAAAATTAAAATTTTATTTTTTTTTTTTTTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATTTT‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAAtt                                                               <  1:27676/125‑1 (MQ=255)

                                                               aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTAAAATGGAAATTAATAtttt                                                             >  1:3270040/1‑128 (MQ=255)

                                                                 ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑TTAAAATATAAAAATAATATTTa                                                            >  2:1543011/1‑128 (MQ=255)

                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATTTAAATAAAAATTTTatt                                                          >  2:1275597/1‑126 (MQ=255)

                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTATTTTTTTTT‑TTATAATGAAAATATAAATtatatta                                                         >  1:212307/1‑125 (MQ=255)

                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑‑TTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGTTAtt                                                      >  2:1845105/1‑128 (MQ=255)

                                                                   attaataaAATAAAACTATTATTTTTTTTTTTATTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATTGC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGt                                                         <  2:275914/126‑1 (MQ=255)

                                                                   aGTAATAAACAAAAACAATTATTATTTTATAATTTTTTTGTTTATGCAAATTTTTATAAAATTAATTTTTACTTTTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGt                                                         <  2:735841/128‑1 (MQ=255)

                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTT‑TTATAATTTAAATATAATTTTTattta                                                        >  2:3122135/1‑124 (MQ=255)

                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTAAAATGAAAATATAAATTTTAGTTa                                                        >  1:3108652/1‑128 (MQ=255)

                                                                     taataaAATAAAAATTTTTTTTTTTTTTATATTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGt                                                         <  2:2097047/128‑1 (MQ=255)

                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTT‑TTATATTGTAAATATAAATTTTAGTTAttt                                                     >  1:3082897/1‑128 (MQ=255)

                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑TTATAATTTAAATATAAATTTTATGTAatt                                                     >  2:190005/1‑128 (MQ=255)

                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TAATAATTGAATTATAAATTTTATTTAtttt                                                    >  2:859892/1‑128 (MQ=255)

                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTTATTATATT‑‑AATTATAATTTTTATTTAttttt                                                   >  1:1807279/1‑127 (MQ=255)

                                                                            ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑‑T‑TTTTTTTTTTTTTTT‑TTAAAATTAAAAAATAAATTTTattaatatttttt                                                >  2:2319272/1‑116 (MQ=255)

                                                                            ataaaaattTTATATTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTc                                              <  1:136242/127‑1 (MQ=255)

                                                                             aaaaaataTTATCATTTTATTTTTTTTTTTTATATTCAAATTTTTAAAAAAATAAATTTAAATTTTAAAAAATATC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                               <  2:1696117/127‑1 (MQ=255)

                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑‑T‑TTTTTTTTTTTTTTT‑TTATAAATAAAATATAAATTTTATTTATtttttttta                                              >  2:570693/1‑125 (MQ=255)

                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTATATTTTAAAAATAAATTTTAGTTATtttttttta                                              >  2:3006718/1‑125 (MQ=255)

                                                                               aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATTTAATTAAAAATTTAAGttttttttttttat                                             >  2:530914/1‑124 (MQ=255)

                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTT‑TTATAATTTAAAAATAAATTTTATGTATTTTTTTTTATAATTa                                        >  2:630334/1‑128 (MQ=255)

                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTATATTGGAAATTAAAATTTTAGGTTTTTTTTTGTATAAGTa                                        >  1:2674218/1‑128 (MQ=255)

                                                                                     ttatTTTTTTATATTTTTTTTGTTTATTTAAATTTTTAAAAAATTTTTTTTTAATTTTTAAAAAT‑‑T‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATATATTTTAGGTATTTTGTTGTCTAAGTATc                                      <  1:9030/128‑1 (MQ=255)

                                                                                      tatttttttattttttTTTTTTTTTTGAAAATTTTTTAAAAATTTATTTTAAATTTTTTAAAATA‑T‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                      <  2:2255205/128‑1 (MQ=255)

                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTT‑TTATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTat                                   >  1:3198113/1‑127 (MQ=255)

                                                                                       atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTAAAATTGAAATATAAATTTTAGGTTTTTTTTTGACTATGTaaat                                     >  1:2987203/1‑125 (MQ=255)

                                                                                          tttttATTTTTTTTTTTTTTATTTAAATTTTTAAAAAATTTATTTTTAATTGTTAAAAAT‑‑T‑TG‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaa                                 <  1:430016/128‑1 (MQ=255)

                                                                                             ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGTTATTTTTTTGTATAATTATCTaaaaaa                               >  1:2516176/1‑128 (MQ=255)

                                                                                             ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTT‑TTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATATaaaaaaa                              >  2:1165579/1‑128 (MQ=255)

                                                                                               ttattttttttGTTTATTTTAATTTTTTAAAAAATTATTTTAAATTTTAAAAAATA‑‑‑TC‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                            <  2:2002889/123‑1 (MQ=255)

                                                                                               tatttttttttttttATTAAAATTTTAAAA‑AATTTATTTGAAATTTTAAAAAAAA‑‑‑‑T‑CTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                          <  1:775775/123‑1 (MQ=255)

                                                                                                 tttttttttttttttGAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAA‑‑‑TC‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                          <  2:3212072/125‑1 (MQ=255)

                                                                                                    ttttttttATATGCAAATTTTTAAAAAATTTTATTGTACTTGTAAAAAATA‑‑‑TG‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                       <  1:2077820/128‑1 (MQ=255)

                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑TTATAATGAAAATATAAATTTTATTTATTTTTTTGTATAATTATATAAAAAAATAGaaataa                     >  2:2616403/1‑125 (MQ=255)

                                                                                                         tttttatttaaaTTTTTAAAAAAATAATTTTAAATTTTAAAAAAT‑TT‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  2:2051499/119‑1 (MQ=255)

                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑AAAAATAAAAAATAAAATTTTTAAATATTTTTTTAAAAAATTATATAAAAAAATaaataaaaa                    >  1:1558485/1‑120 (MQ=255)

                                                                                                          tttttttttaaTTTTTATAAAATTAATTTTAAATTTTTAAAAAT‑TT‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                  <  2:1486009/119‑1 (MQ=255)

                                                                                                          gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATATAAAAAAATTGAAAGGaaaaaa                >  1:1851901/1‑128 (MQ=255)

                                                                                                           tttaagCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑C‑GT‑GTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa                 <  1:2300053/123‑1 (MQ=255)

                                                                                                           tataTTAAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATA‑‑‑TC‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaaa                <  2:2713706/128‑1 (MQ=255)

                                                                                                           tataTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTT‑TTATAATTGAAATATAAAATTTAGTTATTTTTTTGTAAAAATAACAAAAAAAAAAAAAAGGaaaaaa                >  1:2011822/1‑128 (MQ=255)

                                                                                                             ttttaaaaTTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑T‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat               <  1:678692/123‑1 (MQ=255)

                                                                                                              atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑TTATAAAGAAAATAAAAATTTTAGTTTATTTTTTTAATAATTATAAAAAAAAATAAAAAAAAAAAAat               >  1:1061933/1‑128 (MQ=255)

                                                                                                              atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTAAAATGAAAATATAAATTTTTTTTTTTTTTTTTTATAATTAAATAAAAAAATAAAAAGAAAAAAata              >  1:836965/1‑128 (MQ=255)

                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTAAAATGGAAAAATAAATTTTAGTTATTTTTTTGTAAAATATTGTAAAAAATTAAAATAAAAAAAatat             >  1:1634601/1‑128 (MQ=255)

                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑T‑TTTTTTTTTTTTTTT‑TTAAAATGGAAATATAAATTTTTGTTATTTTTTTTTATAATTATATAAAAAAATAAAAATTAAAAAAttttt           >  1:2212595/1‑127 (MQ=255)

                                                                                                                  aaaTTTTTTAAAAAATAATTTTAAATTTTAAAAAATA‑‑‑TC‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGtt         <  2:3083470/128‑1 (MQ=255)

                                                                                                                   aaTTTTTAAAAATTTAATTTGTAATTTTAAAAAAT‑TT‑GT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGtt         <  1:2913057/128‑1 (MQ=255)

                                                                                                                          aaaaaaTTTATTTTTAATTTTTAAAAATTTT‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt   <  1:2802613/128‑1 (MQ=255)

                                                                                                                          aaaaaaTTAATTTTAATTTTTAAAAAATATC‑‑T‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa  <  2:2786395/128‑1 (MQ=255)

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TT‑TTTTTTTTTTTTTTT‑TTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165097‑165347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`