Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,801 A→G intergenic (‑110/+61) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,8010AG97.1% 3282.5 / NA 970intergenic (‑110/+61)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (3/1);  major base G (428/507);  minor base T (20/8);  total (454/516)
Fisher's exact test for biased strand distribution p-value = 1.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1097 total aligned reads displayed.

AAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484673‑484925
                                                                                                                                 |                                                                                                                            
   aaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCa                                                                                                                            >  1:831157/1‑128 (MQ=255)
           aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAaca                                                                                                                    >  2:794465/1‑128 (MQ=255)
              cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                                 >  2:2375580/1‑128 (MQ=255)
               tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                                <  1:929347/128‑1 (MQ=255)
                cATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaa                                                                                                               >  1:2165755/1‑128 (MQ=255)
                 aTAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                              >  1:386292/1‑128 (MQ=255)
                  tAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                             <  2:1595816/128‑1 (MQ=255)
                      tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                         <  2:619861/128‑1 (MQ=255)
                        tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                       >  2:1787455/1‑128 (MQ=255)
                            ccTTTTATAGTTGACCAAAAAATATGATAGATCATAGGAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                                   <  1:119431/128‑4 (MQ=255)
                            ccTTTTATAGTAGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                                   <  1:131420/128‑4 (MQ=255)
                              ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                                 <  2:724209/128‑1 (MQ=255)
                              attaataGTTGACCAAATAATAAGATAGACCATAGCAGAAGCTCTTAAGAACTAAACAACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                                 <  2:355396/124‑1 (MQ=255)
                               tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                                >  1:1111001/1‑128 (MQ=255)
                               tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTATTTTTTAATATAAAAATCAATTAAACACACAAAAACTAACAAATTAt                                                                                                >  1:228442/1‑128 (MQ=255)
                               attctaGTTGAACAAAAAATAAGATAGAGCATAGAAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                                <  1:1003894/124‑1 (MQ=255)
                                 tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACCCTCGATTATTTAATTATATTTTTTTTTAAGATAAAAAGCAATTAAACACACAAAAACTCACCCATTAggg                                                                                              >  2:1399366/1‑128 (MQ=255)
                                    aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTACACTACACTCGATTAATTAATTATATATTTTTTTAATATAAAAAGCAATAAAACACACAAAAACACACCCATTATgataa                                                                                           >  2:595312/1‑124 (MQ=255)
                                    aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAACCTCACCCATTATGGTGa                                                                                           <  2:1463067/128‑1 (MQ=255)
                                        gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCCCCCATTATGGTGAGttt                                                                                       >  2:518065/1‑128 (MQ=255)
                                        gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAAttt                                                                                       >  2:2463648/1‑128 (MQ=255)
                                            aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                                   >  1:1626932/1‑128 (MQ=255)
                                             aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  1:2425198/128‑1 (MQ=255)
                                             aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  2:1500008/128‑1 (MQ=255)
                                             aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  1:479383/128‑1 (MQ=255)
                                              aaaaTAATATAGATCATAGCACTATCACTTAACAAATAAAATACACTCGAATATTTAATTATATTTTTTTTTTATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                                 <  1:1812706/128‑1 (MQ=255)
                                                    aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCTATTAAACACACAACAACTACCCCATTATCGTGAGATTAAAAATTATACa                                                                           >  1:1225365/1‑128 (MQ=255)
                                                    aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                           >  1:845865/1‑128 (MQ=255)
                                                    aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                           >  2:2431231/1‑128 (MQ=255)
                                                     gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACAATCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                                         <  1:1556659/128‑1 (MQ=255)
                                                      aTAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                                         >  2:2149629/1‑128 (MQ=255)
                                                        agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                                       <  1:701617/128‑1 (MQ=255)
                                                        agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACAAATTATAGTGAATATAAAAATAATTCaaaaa                                                                       >  2:1018906/1‑128 (MQ=255)
                                                        agagCATAGCAGAAGCTCTTAACAAATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                                       <  2:2188888/128‑1 (MQ=255)
                                                          agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                                     >  1:167749/1‑128 (MQ=255)
                                                           gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAAATAAACACACAAAAACACAAACATTATGGTGAGTTTAAAAATTAATAAAAAAta                                                                     >  1:330363/1‑127 (MQ=255)
                                                           gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                                    >  1:2120859/1‑128 (MQ=255)
                                                           gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATATAATTATATTTATTTTTAATAAAAAAATCAATTAAACACACAAAAACACACCCATTATGGATAGTTTAAAAAATATTCAAAAAtaa                                                                    >  1:1590050/1‑126 (MQ=255)
                                                            aaTAGCAGAATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTg                                                                   <  2:823658/127‑1 (MQ=255)
                                                             aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                                  >  1:445019/1‑128 (MQ=255)
                                                              tacgagatgatctTAACAACTAAACTACTCTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                                 <  2:92478/118‑1 (MQ=255)
                                                                  aGAAGCTCTTAACAACTAAAATAAACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                             <  2:110274/128‑1 (MQ=255)
                                                                     aGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTa                                                          >  1:778687/1‑128 (MQ=255)
                                                                     aGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTa                                                          <  2:675230/128‑1 (MQ=255)
                                                                      gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                                         >  1:550869/1‑128 (MQ=255)
                                                                      gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                                         <  2:1649836/128‑1 (MQ=255)
                                                                      gCTCTTAACAACTAAACTACACTCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                                         <  1:1074888/128‑1 (MQ=255)
                                                                        tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                                       >  2:2048957/1‑128 (MQ=255)
                                                                          ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                                     >  1:736626/1‑128 (MQ=255)
                                                                           tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTAATCAAAAATTTGATTATTTTAAATCaa                                                    >  1:1266655/1‑128 (MQ=255)
                                                                             acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  1:2376227/128‑1 (MQ=255)
                                                                             acaacTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATATAAAGCAATAAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTAAAAAATATGATTATTTTAAATCatat                                                  >  2:1852001/1‑125 (MQ=255)
                                                                             aaaactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  2:1198898/126‑1 (MQ=255)
                                                                              caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATCGTGAGTTTAAAAATTTATCAAAAATTTCATTATATTAAATCAATTg                                                 >  2:2259603/1‑128 (MQ=255)
                                                                               aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                                >  2:481076/1‑128 (MQ=255)
                                                                                actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCaa                                                    <  2:169252/123‑1 (MQ=255)
                                                                                actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               <  1:522345/128‑1 (MQ=255)
                                                                                   aaactaCACTCGATTAATTAATTATATTTTTTTTTAATATATAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                            <  2:1735698/128‑1 (MQ=255)
                                                                                     actaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcc                                          <  1:134295/128‑1 (MQ=255)
                                                                                      ctaAACTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                                         <  1:898982/128‑1 (MQ=255)
                                                                                       taCCCTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                                        <  2:1902418/128‑1 (MQ=255)
                                                                                       taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAATAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                                        <  1:1445624/128‑1 (MQ=255)
                                                                                       acacgagttttatttatTTTATTTTTTTTTAATATAAAAAGCAATTAAACACAATAAAACTCAAACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                       <  2:1546649/119‑1 (MQ=255)
                                                                                        actctagaatattTAATTCTATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTATAAATCAATTGAAGCTCCCGc                                       <  1:2449983/119‑1 (MQ=255)
                                                                                            tCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                                   <  2:1249725/128‑1 (MQ=255)
                                                                                              gATTATTATATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                                 <  2:555752/128‑1 (MQ=255)
                                                                                                  attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                             <  1:2070164/128‑1 (MQ=255)
                                                                                                   tttAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  2:1438796/128‑1 (MQ=255)
                                                                                                    ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  1:1500188/128‑1 (MQ=255)
                                                                                                    tataTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  2:311633/125‑1 (MQ=255)
                                                                                                     tAATTATATTGTTTTATAAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  1:176965/128‑1 (MQ=255)
                                                                                                      taTTATATTTTATTTTAATATTAAAATTAATTAAAAACACAATAACTCACCCATTTAGGTTTGTTTATATATTTTTCAAAAATTTGATTATTTTAAATATATTGAAGCTCCAGCTTCAACTAAttttg                         <  1:583601/127‑3 (MQ=255)
                                                                                                        ttttatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt                       <  2:889637/125‑1 (MQ=255)
                                                                                                          atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     <  2:2321970/128‑1 (MQ=255)
                                                                                                           tatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTc                    <  1:2327405/128‑1 (MQ=255)
                                                                                                            atTTTTTTTTAATATAAAAAGCATTTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAATATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCa                   >  1:1053869/1‑128 (MQ=255)
                                                                                                            atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTTTTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCa                   >  2:853304/1‑128 (MQ=255)
                                                                                                            atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTAAGCTATCa                   >  1:1409641/1‑128 (MQ=255)
                                                                                                             ttttttattaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt                  <  1:1724425/128‑1 (MQ=255)
                                                                                                             agtttttttaATATAATAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt                  <  2:262643/126‑1 (MQ=255)
                                                                                                               tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAGTAATTTAGATTTCAttt                >  2:2234613/1‑128 (MQ=255)
                                                                                                               tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt                <  2:764724/128‑1 (MQ=255)
                                                                                                                ttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttc               <  1:1148245/128‑1 (MQ=255)
                                                                                                                 tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct              >  1:982321/1‑128 (MQ=255)
                                                                                                                   ttttaatataaaaatCAATTAAACACACAAAAAATCAACCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttctt             <  2:1146078/119‑1 (MQ=255)
                                                                                                                   ttttaatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGACGCTCCAGCTTCAACTAATATAGCTTTCATttctt             >  2:1303614/10‑128 (MQ=255)
                                                                                                                    tttaatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttcttc            >  2:1994489/9‑128 (MQ=255)
                                                                                                                    tttaatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttcttc            >  1:64945/9‑128 (MQ=255)
                                                                                                                       aatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGc         >  1:673091/6‑128 (MQ=255)
                                                                                                                        atatcaaaagCAATGAAAAACTCAAAAACTCACCCATGATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGct        <  2:1051937/123‑1 (MQ=255)
                                                                                                                        atataaaaagCAATTAAACACACAAAAACTCACCCATTATTGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGct        <  1:290467/124‑1 (MQ=255)
                                                                                                                          ataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCACCTAATTTAGCTTTCATTTCTTCAGcttc      >  2:954609/3‑128 (MQ=255)
                                                                                                                            aaaaagCAATTAAACACACAAAAACTCCCCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTGCAAGTAATTTAGCTTACTTTTCTGCAgctgctg    >  2:1366802/1‑127 (MQ=255)
                                                                                                                            aaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTTAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttctt    >  1:1884075/1‑128 (MQ=255)
                                                                                                                            aaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttctt    >  2:416231/1‑128 (MQ=255)
                                                                                                                            aaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttctt    >  1:1996105/1‑128 (MQ=255)
                                                                                                                              aaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTtcttct  <  2:1669208/128‑1 (MQ=255)
                                                                                                                              aaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTtcttct  <  1:871250/128‑1 (MQ=255)
                                                                                                                              aaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTtcttct  <  1:2239788/128‑1 (MQ=255)
                                                                                                                              aaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTtcttct  <  1:1925155/128‑1 (MQ=255)
                                                                                                                                 |                                                                                                                            
AAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484673‑484925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: