BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	32,707	A→T	I1067F (ATT→TTT)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	32,707	0	A	T	99.6%	3595.5 / NA	1158	I1067F (ATT→TTT)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (2/3); new base T (602/550); total (604/554)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1188 total aligned reads displayed.

TTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGAAAC  >  CP016816/32582‑32834

ttACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCAtttttt                                                                                                                               <  1:67532/128‑1 (MQ=255)

  aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                             >  2:965082/1‑128 (MQ=255)

      aaCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                         >  2:1840792/1‑128 (MQ=255)

        ccTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTa                                                                                                                       >  2:1644154/1‑128 (MQ=255)

        ccTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTa                                                                                                                       >  2:1758190/1‑128 (MQ=255)

            tAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                   <  2:875244/128‑1 (MQ=255)

             aaCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTaa                                                                                                                  >  2:2149846/1‑128 (MQ=255)

              aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACTGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                                 <  2:2364210/128‑1 (MQ=255)

                  aggttgttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGCTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGt                                                                                                             <  1:2075809/125‑1 (MQ=255)

                  aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGt                                                                                                             <  1:2220846/128‑1 (MQ=255)

                    tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGa                                                                                                           >  1:181091/1‑128 (MQ=255)

                    tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTATAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAGCAAGTGa                                                                                                           >  2:1047098/1‑128 (MQ=255)

                        ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtct                                                                                                       >  2:62078/1‑128 (MQ=255)

                          ggAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCg                                                                                                     <  2:926797/128‑1 (MQ=255)

                           gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                    >  1:2347061/1‑128 (MQ=255)

                            agaACTTTTCAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGATAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGaa                                                                                                   <  2:1583199/128‑1 (MQ=255)

                            agaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGat                                                                                                   >  2:1411084/1‑127 (MQ=255)

                            agaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGaa                                                                                                   <  1:121461/128‑1 (MQ=255)

                             gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                                  <  1:1006034/128‑1 (MQ=255)

                              aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                                 <  2:1673834/128‑1 (MQ=255)

                                cTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAGATCTTATTTAACAAGTGAATCTCGAAGAGc                                                                                               >  1:2014169/1‑128 (MQ=255)

                                cTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGc                                                                                               <  1:2313030/128‑1 (MQ=255)

                                 ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                              <  1:267222/128‑1 (MQ=255)

                                 ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                              <  2:1146367/128‑1 (MQ=255)

                                  tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGTTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATACTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                             <  1:334174/128‑1 (MQ=255)

                                  tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                             <  2:57996/128‑1 (MQ=255)

                                   ttAATAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                            <  2:1947648/128‑1 (MQ=255)

                                   ttAAAAACTATTTTTAAAGATAACGTTCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                            <  1:1277499/128‑1 (MQ=255)

                                    tAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCaa                                                                                           >  1:159763/1‑128 (MQ=255)

                                     aaaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGATAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAt                                                                                          <  1:156617/128‑1 (MQ=255)

                                      taaaCTATTTTAAAAGATAAAGTGCAAACTACAGGTGAGAGAAAAGAAAACATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtt                                                                                         <  2:1534843/127‑1 (MQ=255)

                                      aaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCAtttttt                                                                                                                               <  1:1510766/90‑1 (MQ=255)

                                      aaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTATCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtt                                                                                         >  2:425799/1‑128 (MQ=255)

                                      aaaaATATTTTTATAGATAAAGTGCAAACTACAGGGGAGTGAAACGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtt                                                                                         <  2:482179/128‑1 (MQ=255)

                                       aaaCTCTTTTTAAAGATAAAGTGCAATCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAttt                                                                                        <  1:1415918/128‑1 (MQ=255)

                                       aaaCTATTTTTAAAGCTAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAttt                                                                                        <  1:1307483/128‑1 (MQ=255)

                                        aaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTATGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtttt                                                                                       >  1:483178/1‑128 (MQ=255)

                                        aaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTTTTTAACAAGTGAATCTCGAAGAGCTCAAgttt                                                                                       >  2:2410005/1‑128 (MQ=255)

                                         aCTATTTATAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                      <  1:1344633/128‑1 (MQ=255)

                                          cTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCt                                                                                     >  1:1100295/1‑128 (MQ=255)

                                            aTTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGCGCTCTATTTTCttt                                                                                   >  2:1001926/1‑128 (MQ=255)

                                            aTTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCttt                                                                                   >  2:652555/1‑128 (MQ=255)

                                             tttttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtttt                                                                                  >  1:564309/1‑128 (MQ=255)

                                              ttttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTg                                                                                 >  2:2265222/1‑128 (MQ=255)

                                              ttttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTg                                                                                 >  2:526377/1‑128 (MQ=255)

                                               tttAAAGATATAGTTCAAACTTGAGGTGTGTGAAAAGATAAGATCTTAATCTTATTTTTAGGTTTTTCTCGTTCATTTTTTAATTCTTATTTAACAATTGATTCTAGAAGAGCTCAATTTTCTTTTgg                                                                                <  2:2454863/128‑1 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTCTTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                                <  2:342140/128‑1 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgt                                                                                >  1:312861/1‑127 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                                >  1:1377612/1‑128 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                                <  1:1731959/128‑1 (MQ=255)

                                                ttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGt                                                                               >  2:1995029/1‑128 (MQ=255)

                                                         aaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATATTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                      <  1:2194728/128‑1 (MQ=255)

                                                          aGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                     >  1:1138789/1‑128 (MQ=255)

                                                              cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                             <  1:1045946/100‑1 (MQ=255)

                                                              cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaaac                                                                 >  1:226429/1‑128 (MQ=255)

                                                               aaaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACt                                                                <  2:2105376/128‑1 (MQ=255)

                                                                aaCTACAGGTGAGTGAAAAGAAAAGATCTTCAACTTAATTTTAGGTTATTCTCCTTCATTTTTTTAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                               <  2:2224503/128‑1 (MQ=255)

                                                                  cTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACtata                                                             >  1:2138859/1‑128 (MQ=255)

                                                                   tACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATaa                                                            >  2:1804899/1‑128 (MQ=255)

                                                                    aCAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAAt                                                           <  1:79478/128‑1 (MQ=255)

                                                                      aGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTc                                                         >  2:1458531/1‑128 (MQ=255)

                                                                       ggTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTcc                                                        <  1:918734/128‑1 (MQ=255)

                                                                          gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcaca                                                     >  2:1124403/1‑128 (MQ=255)

                                                                              gaaaagaaaaGATCTTAAACTTAATTTTAGGTTATTCTAATTCATTTTTTAAATCTTATTTAACAAGTGAATATCTAAGAGCTCAATATTCTTTTGTTTGAAAAAAACATACTATAATTCAACaaaaa                                                 >  2:2328173/1‑128 (MQ=255)

                                                                                aaagaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCAACAAAAAGa                                               >  1:757814/1‑128 (MQ=255)

                                                                                 aagaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGAt                                              >  2:3774/1‑128 (MQ=255)

                                                                                  agaaaagaTCTTAAACTTAATTTTCGGTTATTCTCCATCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATc                                             <  1:511736/128‑1 (MQ=255)

                                                                                  agaaaagaTCTTAAACTTAATTTTAGATTATTCTCCTTCATTTTTTAAATCTTATTTAAAAAGAGAATCTCTAAGAGATCAATTTTCTTTTTGTTTACAAAAAAAAACTATAATTCCAAAAAAAGATc                                             >  2:844552/1‑128 (MQ=255)

                                                                                      aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                         <  2:1716284/128‑1 (MQ=255)

                                                                                      aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                         <  2:782213/128‑1 (MQ=255)

                                                                                      aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                         >  1:1846216/1‑128 (MQ=255)

                                                                                        gaTCTTATACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                       <  1:1968479/128‑1 (MQ=255)

                                                                                        gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                       <  1:1780178/128‑1 (MQ=255)

                                                                                        gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                       <  1:2380737/128‑1 (MQ=255)

                                                                                        gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAATTTCTTTTGGTTGACAAAAGCAAACTATAATTCCACAAAAAGATCAATTAg                                       >  2:1287653/1‑128 (MQ=255)

                                                                                          tCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATTAATTAGCa                                     >  1:721763/1‑128 (MQ=255)

                                                                                            ttAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCATTTAGCAAc                                   >  1:1676896/1‑128 (MQ=255)

                                                                                            ttAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAAc                                   >  1:1238833/1‑128 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTATCAACt                                  >  2:1208088/1‑128 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                  >  1:1699253/1‑128 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                  >  2:2054344/1‑128 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                  >  1:2447282/1‑128 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                  >  2:724893/1‑128 (MQ=255)

                                                                                                  ttAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                      <  2:1260488/71‑1 (MQ=255)

                                                                                                   tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                            >  2:287781/1‑128 (MQ=255)

                                                                                                       tttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAACCAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAc                        >  2:1351222/1‑128 (MQ=255)

                                                                                                        ttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAACAGATCAATTAGCAACTATTTTTAAAcc                       >  1:420988/1‑128 (MQ=255)

                                                                                                         tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGATGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTACACAAAAAGATCAATTTGCAACTATTTTTAAAccg                      >  2:698788/1‑127 (MQ=255)

                                                                                                         tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCt                      >  1:2408696/1‑128 (MQ=255)

                                                                                                             ttATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAAt                  <  1:1894314/128‑1 (MQ=255)

                                                                                                             ttATTCTACTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAAt                  <  1:868127/128‑1 (MQ=255)

                                                                                                               aTTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCa                <  2:1899584/128‑1 (MQ=255)

                                                                                                                ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa               >  1:2041092/1‑128 (MQ=255)

                                                                                                                ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa               >  1:954181/1‑128 (MQ=255)

                                                                                                                   tcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATa            <  1:1923549/128‑1 (MQ=255)

                                                                                                                   tcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATa            <  1:1644154/128‑1 (MQ=255)

                                                                                                                    ccTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATaa           <  2:1082658/128‑1 (MQ=255)

                                                                                                                    ccTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATaa           <  1:62078/128‑1 (MQ=255)

                                                                                                                     cTTCATTTTTTAAATCTTATTTAACAAGTGAATCTAGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAAc          <  1:1462724/128‑1 (MQ=255)

                                                                                                                      ttCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGCGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTATCAACTATTTATAAACCTAAATCAAAAAACa         >  2:2292084/1‑128 (MQ=255)

TTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGAAAC  >  CP016816/32582‑32834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`