Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT96.8% 2844.5 / 2.1 1027intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (8/25);  new base T (669/325);  total (677/350)
Fisher's exact test for biased strand distribution p-value = 7.97e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1073 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAA  >  CP016816/165093‑165343
                                                                                                                              |                                                                                                                                
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                <  1:1111397/128‑1 (MQ=255)
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                <  1:400051/128‑1 (MQ=255)
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTACATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                >  1:787854/1‑128 (MQ=255)
  tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGAATATGCAAATTTTtaaaa                                                                                                                              <  2:1293889/128‑2 (MQ=255)
   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                             >  2:1473702/1‑126 (MQ=255)
   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                             <  1:2365662/128‑3 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAATTTTTAAAAaat                                                                                                                           >  2:1442195/1‑128 (MQ=255)
      ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatt                                                                                                                         >  1:902872/1‑128 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAGCTATTATCTTTTTAGATAATTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                        >  2:819078/1‑128 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                        <  1:2121239/128‑1 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                        >  2:1647803/1‑128 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTGTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                        >  2:1206100/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATATTTAAAAaattaat                                                                                                                      >  2:2164517/1‑128 (MQ=255)
          ttttATTAATTTACTAGTTGATAATATTTAGTTTAATTAATTTATCGCAATTGTATGAATTAGTAAGTAACTAAAACTTTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaatt                                                                                                                     <  2:1817130/125‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                    <  1:2425322/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                    <  2:1969909/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCAATAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                    <  1:1003197/128‑1 (MQ=255)
            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                   <  1:2076568/128‑1 (MQ=255)
            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                   >  1:2055290/1‑128 (MQ=255)
             tATTACTTTACTAGTTTATAATATTTAGATTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                  <  2:1269397/128‑1 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATAGCACTTGTATGAATTATTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAAAATTAATTTGa                                                                                                                  >  1:610322/1‑128 (MQ=255)
              aatacattacaAGTCGATAATATATAGATTCATAAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                 <  2:636335/126‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                 >  2:95911/1‑128 (MQ=255)
                 actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                              >  1:239345/1‑128 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                          <  1:1378304/128‑1 (MQ=255)
                       ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTaaaa                                                                                                        <  1:1205687/128‑1 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTaaaaa                                                                                                       >  1:52660/1‑128 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGAACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                       <  1:1896723/128‑1 (MQ=255)
                          gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAt                                                                                                     >  2:765835/1‑128 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                    >  2:2061358/1‑128 (MQ=255)
                               taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATTTGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATATCtt                                                                                              >  2:1550899/1‑128 (MQ=255)
                                aagatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGAGATTTTTTGTATATGCAAATTTTTAATTAATTAATTTGAACTTGTAATAAATAGCtgt                                                                                             >  2:2129334/4‑126 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                            >  1:2329069/1‑128 (MQ=255)
                                    tttATTTTAATTAATTTTTAGCAATTTTTATAATTATTAATAAAATAAAAAAATTATCTTTTTTTTTATTTTTTTTATTTTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGCttttttt                                                                                         <  2:53914/128‑1 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                       >  1:720406/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                    >  1:1431684/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTCAACTTGTAAAAAATAGCttttattttttt                                                                                    >  2:104199/1‑128 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                  >  1:1728929/1‑128 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                  >  1:1582257/1‑128 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGTAAAAAATAGCtttttttttttttt                                                                                  >  2:510832/1‑128 (MQ=255)
                                           tCATTAATATATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATAATTTTTGTATATGCAAATTATTAAAAAATTAATTTGCAATTTTAAATAATATCttttaatattattt                                                                                  >  2:250524/1‑118 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                 >  2:882967/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                >  1:1172467/1‑128 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttttt                                                                           >  2:306879/1‑126 (MQ=255)
                                                    tATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATaa                                                                         >  2:353410/1‑128 (MQ=255)
                                                     aTCGCACTTGTATTTATTATTAATAAACTAAAAATATTATGTTTTTAGAAATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATg                                                                       <  2:1908954/128‑1 (MQ=255)
                                                     aTCGCAATTGTATGAATTATTAATAAACTAAAAATATTATTTTTTTATTTATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATATCtttttttttttttttatataatgg                                                                        <  2:842087/128‑10 (MQ=255)
                                                      tCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAATACATAGCtttttttttttttttttattaattc                                                                       >  2:681638/1‑120 (MQ=255)
                                                       ctaaattttattaattattaatTAAATAAAAATATTATCTTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGa                                                                     <  1:432965/120‑1 (MQ=255)
                                                        gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTatattttaaa                                                                     >  1:1961168/1‑122 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTTTAATTGAAatta                                                                >  1:1060446/1‑126 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAAttaaataa                                                                 >  1:683244/1‑121 (MQ=255)
                                                             tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataattaaaaaataa                                                                >  2:1763320/1‑116 (MQ=255)
                                                                 tGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATTTAAATATAAAttt                                                          >  1:1537938/1‑128 (MQ=255)
                                                                  gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAAttt                                                          >  1:1473320/1‑128 (MQ=255)
                                                                   aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAAtt                                                           >  1:1155242/1‑128 (MQ=255)
                                                                    ttttttaataaAATAAAAATATTTTATTTTTATATATTTTTTTTAAATTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                       <  1:1788069/123‑1 (MQ=255)
                                                                     ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATTAT‑‑TGAAATATAAATTTTAgtt                                                     >  1:2279917/1‑126 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAATATAAATAAAAAtttatg                                                       >  1:2459166/1‑125 (MQ=255)
                                                                       tttaataaAATAAATATATTTTTTTTTTAGATATTTTTTTTAAATGCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                     <  2:1596763/126‑1 (MQ=255)
                                                                        gttaagaattaaaacttTTTTCTTTTTAGTTATTTTTTTTTTATTTAAATTTTTAAATAAATTATTTCTAATTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                     <  2:449982/119‑1 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTAttttt                                               >  1:839666/1‑127 (MQ=255)
                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATGTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTTAATATAAATTTTTGGTAtttttt                                              >  2:493765/1‑126 (MQ=255)
                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAATTTAAATTTTATGAATtttttt                                             >  1:1248621/1‑128 (MQ=255)
                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAATGAAATATAAATTTTATGTtttttttttt                                           >  2:2382358/1‑126 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATtttttt                                             >  2:1689390/1‑128 (MQ=255)
                                                                                 taaaactaTGATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGAAAAAAATAGCTTTTTTTTTTTTTTTTTGAAAATGGAAAAATAAATTTTAGTAATTtttttga                                           >  2:591537/1‑127 (MQ=255)
                                                                                  aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAATATAAATTTTAGGTATtttttttta                                          >  1:818805/1‑125 (MQ=255)
                                                                                   aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATttttttttat                                         >  1:963980/1‑124 (MQ=255)
                                                                                      aaattattTTTTTATTAAATTTTTGTTTAATCAAATTTTTAAAAAATAAAATTTTAATTTTTAAAAAAATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                      <  1:267738/126‑1 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATGTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGAAAATAAAATTTTTATTTATTtttttgattatttt                                    >  2:1242423/1‑120 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTAAAATATAAATATTATTAATTTTTTTTTATAATTAt                                   >  1:33850/1‑128 (MQ=255)
                                                                                        attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTAATTTTGTTTTATAATTAt                                   >  1:479659/1‑128 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTAATATTTTTTATAAGTa                                    >  1:874414/1‑128 (MQ=255)
                                                                                          tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTCTAATTATc                                  >  1:2015600/1‑128 (MQ=255)
                                                                                           atCTTTTTTTATATTTTTTTTATATTAAAATTTTTAAAAAAATATTTTTTAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                 <  2:1869126/128‑1 (MQ=255)
                                                                                               tttttttttttttttttttttGTAAATTTTTTAAAAATTTATTTTTAATTTTTAAAAAATTGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaa                             <  2:1104567/119‑1 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATATTAGTTATTTTTTTTTATAAGTATCaaaaaaaa                          >  2:1895303/1‑128 (MQ=255)
                                                                                                      tatTTTTTTTATTTTGAAATTTTTTAAAAATTTATTTTAACTTTTTAAAAATTTCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                       <  2:2243951/128‑1 (MQ=255)
                                                                                                       tttttttttttATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                   <  2:1357026/127‑1 (MQ=255)
                                                                                                       atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTCTAAGTATATAAAAAAATATaa                     >  1:1326245/1‑128 (MQ=255)
                                                                                                        ttttttttATTTTCAAATTTTTAAAAAATTTATTTTTAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                    <  2:1401355/128‑1 (MQ=255)
                                                                                                         tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTGTCTAATTATATAAAAAAATAGAAAgt                  >  1:1646682/1‑127 (MQ=255)
                                                                                                          ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTATTTATAATTTAAATAAAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATTGaaatg                  >  1:2245413/1‑126 (MQ=255)
                                                                                                          ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTTTATAAGTATATaaaaaaaattaaagta                 >  2:387034/1‑119 (MQ=255)
                                                                                                            ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTTTTTTGTTTTATAAGTATCTAAAAAAATAAAAATGaa                >  1:1853969/1‑128 (MQ=255)
                                                                                                             tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTTTTTTGTTGTGTAAGTATCTAAAAAAATAGAATGTaaa               >  2:2278137/1‑128 (MQ=255)
                                                                                                               ttaattgaaaTTTTTAAAAAAATAATTTTAAAATGTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa             <  1:300946/121‑1 (MQ=255)
                                                                                                                atatTCAATTTTTTAAAAAAATAAATTTAACTTTTTAAAAATATGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa             <  1:2208454/128‑1 (MQ=255)
                                                                                                                 tatttaaaTTTTTAAAAAATTAATTTTAACTTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATGTAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat           <  1:654110/123‑1 (MQ=255)
                                                                                                                 tatTCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat           <  2:2022576/128‑1 (MQ=255)
                                                                                                                  atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTTTTAATTTAAATATTAATTTTATTTATATTTTTATATAAGTATATAAAAAAATTTAAAAGAAAAAat           >  1:579750/1‑128 (MQ=255)
                                                                                                                    ttaaaTTTTTAAAAAATTAATTTTTTATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata          <  2:1155242/126‑1 (MQ=255)
                                                                                                                    gCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTATAATTATATAAAAAAATTTAAAGGAAAAAATAtt        >  1:168034/1‑128 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTAAAAAATAAATTTAATTTATTTTTTTGTATAATAATATAAAAAAAAAAAAATAAAAAAATAtt        >  1:2173852/1‑128 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTAGGTATTTTTTTTTATAAGTATATAAAAAAATAGAAAGTAAAAAATAtt        >  1:1913140/1‑128 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAAAAAAAATTTTAGTTATTTTGTTGAGTAAGTATAAAAAAAAATAAAAAGTAAAAAATAtttt      >  1:1297375/1‑126 (MQ=255)
                                                                                                                        aTTTTAAAAAATTTAATTTAAAATTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa   <  1:753655/128‑1 (MQ=255)
                                                                                                                        aTTTTAAAAAAATAAATTTATCATTTAAAAAAATTTTATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa   <  2:2422501/128‑1 (MQ=255)
                                                                                                                          ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGAAAATTAAAATTTTAGTTTTTTTTTTGTAAAATTAAATAAAAAAAAAAAAAGTAAAAAATATTTTTaaa  >  2:2312878/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                                
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAA  >  CP016816/165093‑165343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: