Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,163 2 bp→TT intergenic (‑446/+396) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1630AT99.3% 3157.0 / NA 1040intergenic (‑446/+397)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (2/5);  new base T (550/481);  total (552/488)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1640AT98.3% 2755.5 / NA 1028intergenic (‑447/+396)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (4/13);  new base T (540/470);  total (544/484)
Fisher's exact test for biased strand distribution p-value = 2.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1041 total aligned reads displayed.

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420041‑420285
                                                                                                                           ||                                                                                                                              
gattcatACTTTTAGTCTGATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  2:1800069/127‑1 (MQ=255)
aattaatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  2:1556811/127‑1 (MQ=255)
 attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                           >  1:1300773/1‑128 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                          >  1:2375843/1‑128 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                          >  1:1858325/1‑128 (MQ=255)
  atcatACATATACTCTCATAAATCTAAGTTTCTAAAATTAATTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                          <  1:599588/127‑1 (MQ=255)
   tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                         >  1:106076/1‑128 (MQ=255)
   tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                         >  1:2299584/1‑128 (MQ=255)
     atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                      <  1:1380120/128‑1 (MQ=255)
       aCTTTTATTCTCTTAAATCTAAGTTTCTAATATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                    <  1:817907/128‑1 (MQ=255)
       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTATAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                    >  1:1342844/1‑128 (MQ=255)
        cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                   <  1:465509/128‑1 (MQ=255)
        cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                   <  2:130267/128‑1 (MQ=255)
        cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                   <  2:560524/128‑1 (MQ=255)
         ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                  <  1:99090/128‑1 (MQ=255)
           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                >  1:277125/1‑128 (MQ=255)
           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                <  1:93177/128‑1 (MQ=255)
            tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                               <  1:1683356/128‑1 (MQ=255)
             aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                              <  2:487743/128‑1 (MQ=255)
             aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                              <  1:491873/128‑1 (MQ=255)
                ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACGTTTTTAAAAAAATTTTAATTTTAATAAATGAATTATTTTTCa                                                                                                           >  1:68741/1‑128 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                          <  2:1705999/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                          <  2:339010/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                          <  2:836605/128‑1 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                        <  2:1376844/128‑1 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATAAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                        >  2:1040761/1‑128 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATATTAATAACTGAATTATTTTTCAAAtt                                                                                                       >  1:631542/1‑128 (MQ=255)
                    tAAATCTAAGTTTCTAAAATTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                       <  2:2455102/128‑1 (MQ=255)
                     aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATAAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTAAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                      >  1:1827570/1‑128 (MQ=255)
                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                    >  2:2147103/1‑128 (MQ=255)
                        tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTa                                                                                                   >  1:1000261/1‑128 (MQ=255)
                          tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                 <  1:1203818/128‑1 (MQ=255)
                           aaGTTTCATAAATTATTTTATCATTTAAAAAAATAATAGTTTTATTAGTTGATATATTATAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                <  2:1646657/128‑1 (MQ=255)
                              tttCTAAAAATATTTTATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATATTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                             <  1:1549540/128‑1 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                            >  2:151903/1‑128 (MQ=255)
                                tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                           <  1:2156573/128‑1 (MQ=255)
                                tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                           >  2:645301/1‑128 (MQ=255)
                                tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAATTTTAATTTTAATAAATTAATTATTTTTAAAATTGGTAAATTAttt                                                                                           >  2:1186432/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          >  2:203193/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          >  1:1039759/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          <  1:2199670/128‑1 (MQ=255)
                                  tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         >  2:1878561/1‑128 (MQ=255)
                                  tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         <  2:75336/128‑1 (MQ=255)
                                  tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         <  2:387568/128‑1 (MQ=255)
                                   aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                        >  1:901030/1‑128 (MQ=255)
                                    aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                       <  2:2413998/128‑1 (MQ=255)
                                     aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAATCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                      >  2:1454630/1‑128 (MQ=255)
                                     aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                      >  2:2353174/1‑128 (MQ=255)
                                         attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                  >  2:261401/1‑128 (MQ=255)
                                            ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                               >  1:849872/1‑128 (MQ=255)
                                               ttaaatgataaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                            >  1:1517442/10‑128 (MQ=255)
                                               tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCACTACATTATCTa                                                                            >  2:2380929/1‑128 (MQ=255)
                                                  tttAATAAAATTATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTATTAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                         <  2:2300992/128‑1 (MQ=255)
                                                  tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                         >  1:2006318/1‑128 (MQ=255)
                                                   ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCtaataa                                                                        >  1:754579/1‑128 (MQ=255)
                                                      aaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                     >  1:1895930/1‑128 (MQ=255)
                                                       aaaaaTAATACTTTTAATAGTTGATATATTATAAAAATAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                    <  1:1771329/128‑1 (MQ=255)
                                                        aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                                   >  2:2209939/1‑128 (MQ=255)
                                                          aataataatTTTATTAGTTTATATATTAGAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                 <  2:2247584/128‑1 (MQ=255)
                                                          aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                 <  1:1246989/128‑1 (MQ=255)
                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                <  2:1991718/128‑1 (MQ=255)
                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                >  1:256222/1‑128 (MQ=255)
                                                           ataataCTTTTATTAGTTGATATATTAGAAAAATACTAGCACTTTTTTAAAATATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                <  1:1655648/128‑1 (MQ=255)
                                                             aataCTTTTATTAGTTGATATATTATAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAt                                                              <  1:65757/128‑1 (MQ=255)
                                                              ataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAta                                                             >  2:2094211/1‑127 (MQ=255)
                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑ggt                                                         >  1:2334204/1‑126 (MQ=255)
                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTAAAATTTTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCt                                                         >  2:914084/1‑128 (MQ=255)
                                                                  ttttATTAGTTGATATATTAGAAAACTACTAACACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GATCg                                                       >  1:788307/1‑128 (MQ=255)
                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑TCTCGt                                                     >  1:1715582/1‑128 (MQ=255)
                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGt                                                     >  1:1968270/1‑128 (MQ=255)
                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGt                                                     >  1:1110698/1‑128 (MQ=255)
                                                                    ttattaTTTTATATATTAGAAAAATAATAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAATATCAATTAAACAATTTTAT‑‑GCTCGtt                                                    <  1:365639/128‑1 (MQ=255)
                                                                     tattaGTTGATATATTATTAAAATATTAGCAATTTTTTAAAAAATTTTTTATTTTAATAACTTAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGttt                                                   <  1:1153282/128‑1 (MQ=255)
                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑TCTCGttt                                                   >  2:528014/1‑128 (MQ=255)
                                                                           ttGATATATTTGAAAACTATTAGCAATTTTTTAAAAAATTTTTAAATTTAATAACTGAAATTATTTTTAAATTTTTAAATTATTTTCAAATCATTATCTATTAATAAATTTTAT‑‑TCTCGttttttttt                                             <  2:285312/128‑1 (MQ=255)
                                                                                    tAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGtttttttttttttttttt                                    >  1:402772/1‑127 (MQ=255)
                                                                                        aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTaaaaaa                               >  1:726539/1‑128 (MQ=255)
                                                                                         aataaattctctttttttAATATTTTTTAAATTTAATTATTGAATTATTTTTCAAATTTTTAAATTATTATCAAATCATTATTTATAAATAAAATTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTACaaaaa                             <  1:2253451/116‑1 (MQ=255)
                                                                                         aaataataGCTCTTTTTTAAAAAATTTTTAATTTTAATAAATTTATTTTTTTTAAAATTTTTAAATTATTTTCAAATAATTATATATTAATAAATTTTAT‑‑GCTCTTTTTTTTTTTTTTTTTTACaaaa                              <  2:1125260/122‑1 (MQ=255)
                                                                                         aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGtttttttttttttttttttttaa                               >  2:2181444/1‑122 (MQ=255)
                                                                                           ctagtaTCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGtttttttttttttacaaaaatataa                             <  2:598277/128‑13 (MQ=255)
                                                                                           ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTATTTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGtttttttttttttttaaaaaaataa                             >  2:2053628/1‑118 (MQ=255)
                                                                                           ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTACAAAAata                           >  2:1218034/1‑128 (MQ=255)
                                                                                             aaataaaatttttaAAAAAATTTTTAATTTAAATAATTTATTTTTTTTTAAAATTTTTAAATTTTTTAAAAAAATTTTTTATTAATAAATTTTTT‑‑TTTTGTTTTTTTTTTTTTTTTAAAAAAATATaa                        <  2:942614/120‑1 (MQ=255)
                                                                                               tatcaattttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAAAAATTATTTATTAATAAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTACAAAAATATAat                       <  1:1586802/122‑1 (MQ=255)
                                                                                               tatcaattttttAAAAAATTTTTAATTTTAAAAAATTAATTATTTTTAAAATTGTTAAATTATTTTAAAATCATTAAATATTAATCAATTTTAT‑‑TCTCGTTTTTTTTTTTTTTTTTACAAAAATATaa                        <  1:209913/122‑1 (MQ=255)
                                                                                               tatcACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTAATTTAAAATCATTTTATATTAATAAATTTTAT‑‑TCTCGTTTTTTTTTTTTTTTTTACAAAAATATaa                        <  1:2038621/125‑1 (MQ=255)
                                                                                               taccaattttttTAAAAATTTTTAATTTTAAAAAATTTATTATTTTTAAAATTTTTAAAATATTTTAAAAAAAATATATATTAATCAAATTTTT‑‑TCTCGTTTTTTTTTTTTTTTTTACAAAAATATaa                        <  2:693819/122‑1 (MQ=255)
                                                                                                ataaattttttAAAAAATTTTTAATTTTAATAAATGTATTATTTTTCAAAATTTTTAATTATTTTCAAATCATTATAAATTAATAAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTACAAAAATATAat                       <  2:1967618/123‑1 (MQ=255)
                                                                                                aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTAAAaaaataaaat                       >  2:1851107/1‑128 (MQ=255)
                                                                                                 taaattttttAAAAAATTTTTAATTTTAAATAATTAATTTTTTTTTAAATTTTTAAATTTTTTTTAAATAATTATATATTATTAATTTTTTTTATCTTTTTTTTTTTTTTTTTTTTACAAAAATATaa                        <  1:1462396/124‑1 (MQ=255)
                                                                                                  cACTATTTTAAAAAAATTTTAATTTTAATAACTGAATTATTTTTCAAATTCTTAAAATATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑TCTCGTTTTTTATTTTTTTTTACAAAAATAAAattta                    >  1:1941849/1‑128 (MQ=255)
                                                                                                    cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTAAAAAAAAAAAatttat                   >  1:53740/1‑128 (MQ=255)
                                                                                                     ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCTTTTTTTTTTTTTTTTTTAAAAAAAAATAatttatt                  >  1:1436139/1‑128 (MQ=255)
                                                                                                      tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑TCTCGTTTTTTTTTTTTTTTTTAACAAAAATAAAttttatt                  >  2:1244519/1‑128 (MQ=255)
                                                                                                      tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTAAAAAAATATAatttattt                 >  1:2077360/1‑128 (MQ=255)
                                                                                                       ttttAAAAAATTTTTAATTTTAAAAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTAT‑‑TCTCGTTTTTTTTTTTTTTTTTTACAAAAATATAatttattt                 <  1:243054/128‑1 (MQ=255)
                                                                                                           aaaataTTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTTAATTATTTTAAAATAATTATATATTAATAAATTTTAT‑‑TTTATTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTa            <  1:328835/128‑1 (MQ=255)
                                                                                                             aaatTTTTAAATTTAATAATTTAATTATTTTTTAATTTTTTAAATTTTTTTTAATTCATTATATATTTATAAATTTTAT‑‑GCTGGTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa          <  2:1940428/128‑1 (MQ=255)
                                                                                                                     ttaatttatatAATTTAATTTTTTTTATATTTTTTAATTTTTTTTAAAATAATTTTATATTTATAAATTTTAT‑‑TTTATTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATAtt  <  1:960324/126‑1 (MQ=255)
                                                                                                                           ||                                                                                                                              
CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420041‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: