Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 437,057 C→A A214S (GCA→TCA)  JCVSYN2_01985 ← glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816437,0570CA99.7% 6953.0 / NA 2178A214S (GCA→TCA) JCVSYN2_01985glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base A (1162/1008);  minor base T (3/3);  total (1166/1012)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2242 total aligned reads displayed.

ATCTAAAAACTTATAACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGCTGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTTCTTCTAAATGTT  >  CP016816/436932‑437181
                                                                                                                             |                                                                                                                            
atatAAAAACTTATAAAAAGTTTGATAATAATGAGCTTAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTATAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAatgatg                                                                                                                            <  2:2241161/125‑1 (MQ=255)
 tCTAAAAACTTATAAAAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAAtgatga                                                                                                                           <  2:468362/128‑1 (MQ=255)
  cTAAAAACTTATAACAAGTTTGATAAGAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCTTTGTCTTAATATATCAATtgatgat                                                                                                                          >  1:342493/1‑127 (MQ=255)
   tAAAAACTTATAACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAat                                                                                                                         <  1:775281/128‑1 (MQ=255)
    aaaaaCTTATAACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAata                                                                                                                        <  1:4128583/128‑1 (MQ=255)
    aaaaaCTTATAACAAGTATGTTAATAATGATCATAAGTAAATTTATCAATAAGAAGTTTATATTTATTAATTTGACTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAata                                                                                                                        <  1:4444009/128‑1 (MQ=255)
      aaaCTTATAACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATAtt                                                                                                                      <  2:2629433/128‑1 (MQ=255)
          ttATAACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTTATAATATGAGTTCATTGTATAAATAAATCATATGTTAATTTTTTCAt                                                                                                                  >  2:1374433/1‑128 (MQ=255)
            ataACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTa                                                                                                                <  1:146466/128‑1 (MQ=255)
            ataACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTa                                                                                                                >  1:3369075/1‑128 (MQ=255)
            ataACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTa                                                                                                                >  1:2115350/1‑128 (MQ=255)
              aaCAAGTTTGATAATAATGCGCATAAGTTAAATGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATAGACTTAATAAATCAAATGATGAATATTTCATTACt                                                                                                              >  1:4343018/1‑128 (MQ=255)
               aCAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTg                                                                                                             >  2:3721276/1‑128 (MQ=255)
                cAAGATTGATAATACTGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGa                                                                                                            <  2:3940456/128‑1 (MQ=255)
                 aaGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATATATCAAATGATGAATATTTCATTACTGaa                                                                                                           >  1:2926916/1‑128 (MQ=255)
                 aaGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATATCATTACTGaa                                                                                                           >  2:1800078/1‑128 (MQ=255)
                  aGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGaaa                                                                                                          >  2:3639747/1‑128 (MQ=255)
                    tttGATAATAATGATCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAAtt                                                                                                        <  2:2073522/128‑1 (MQ=255)
                    tttGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAAtt                                                                                                        >  2:4340616/1‑128 (MQ=255)
                        ataataATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTAt                                                                                                    >  1:1807326/1‑128 (MQ=255)
                        ataataATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTAt                                                                                                    >  1:3538991/1‑128 (MQ=255)
                         taataaTGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATg                                                                                                   <  1:401451/128‑1 (MQ=255)
                             aatGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAAt                                                                                               >  2:342312/1‑128 (MQ=255)
                              atGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATg                                                                                              >  2:2507025/1‑128 (MQ=255)
                              atGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATg                                                                                              <  1:194663/128‑1 (MQ=255)
                               tGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGa                                                                                             >  1:2782132/1‑128 (MQ=255)
                                gAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGat                                                                                            <  1:2694847/128‑1 (MQ=255)
                                     tAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCAt                                                                                                                  >  1:3177063/1‑101 (MQ=255)
                                      aaGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGataaataa                                                                                      >  1:4621423/1‑128 (MQ=255)
                                       aGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATACTATGTAATGATAaataat                                                                                     >  2:4152468/1‑128 (MQ=255)
                                        gTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTATAAAATAATTAATAAGATTTTTTCATTGTCTTAATAAATCAAATTATGAATATTTAATTAATGAAATTCTATTTAATGATAAATAatc                                                                                    >  1:1699930/1‑128 (MQ=255)
                                         ttAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATATATCAAATGATGAATATTTCATTACTGAAATTGTATGTAATGATAAATAatca                                                                                   >  1:1269379/1‑128 (MQ=255)
                                         ttAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAatca                                                                                   <  1:3933975/128‑1 (MQ=255)
                                          tAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATTTAATGATAAATAatcat                                                                                  >  2:740854/1‑128 (MQ=255)
                                            aTTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAAtcatca                                                                                >  1:4529778/1‑128 (MQ=255)
                                            aTTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAAtcatca                                                                                >  2:1465017/1‑128 (MQ=255)
                                                aTCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGaaa                                                                            <  1:366057/128‑1 (MQ=255)
                                                aTCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGaaa                                                                            <  2:1607039/128‑1 (MQ=255)
                                                 tCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAat                                                                           >  2:527115/1‑128 (MQ=255)
                                                 tCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAat                                                                           >  2:3499074/1‑128 (MQ=255)
                                                   aCTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAatat                                                                         <  1:3875230/128‑1 (MQ=255)
                                                      aagaagTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAAAATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAataaacc                                                                      >  1:1870615/1‑124 (MQ=255)
                                                       agaagTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCa                                                                     <  2:809736/128‑1 (MQ=255)
                                                       agaagTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCa                                                                     >  2:1156796/1‑128 (MQ=255)
                                                          agTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTAATGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGt                                                                  >  1:3298436/1‑128 (MQ=255)
                                                            ttaatatTTATTAATTTGTCTAAAATAATTAATAATATGTGGTAATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTAt                                                                <  2:2420769/125‑1 (MQ=255)
                                                             ttATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATc                                                               >  1:3819469/1‑128 (MQ=255)
                                                                tatttattAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGa                                                            >  1:1129303/1‑128 (MQ=255)
                                                                 atttattAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGaa                                                           >  1:3013599/1‑128 (MQ=255)
                                                                   ttattaATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATa                                                         <  2:3244274/128‑1 (MQ=255)
                                                                     attaATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATAATCAGAAATATAGCAAGTATCTGAATAAg                                                       >  2:4577010/1‑128 (MQ=255)
                                                                      ttaATTTGTCTAAAATAATTAATATTATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGAAAGTAAATGAATAAGa                                                      >  1:2365631/1‑128 (MQ=255)
                                                                       taATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGAt                                                     >  1:666438/1‑128 (MQ=255)
                                                                         aTTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATaa                                                   >  2:4248617/1‑128 (MQ=255)
                                                                         aTTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATaa                                                   <  2:2724363/128‑1 (MQ=255)
                                                                          tttGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCaa                                                                    <  1:1533840/110‑1 (MQ=255)
                                                                          tttGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATaaa                                                  <  2:3369075/128‑1 (MQ=255)
                                                                          tttGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATAAGAAATATAGCaa                                                                    >  2:1533840/1‑110 (MQ=255)
                                                                           ttGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATATCAAGTATCTGAATAAGATaaaa                                                 >  2:3019954/1‑128 (MQ=255)
                                                                               ttAAAATAATTATTAATATGTTGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAAttt                                             <  1:251476/127‑1 (MQ=255)
                                                                               cTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAAttt                                             <  2:3576922/128‑1 (MQ=255)
                                                                                    ataatTAATAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAAGCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAg                                        >  1:1204220/1‑128 (MQ=255)
                                                                                     taattaatAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGt                                       <  1:1069499/128‑1 (MQ=255)
                                                                                      aattaatAATATGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGa                                                            <  2:3868632/106‑1 (MQ=255)
                                                                                            taataTGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGtaaataaa                                >  2:2558360/1‑128 (MQ=255)
                                                                                            taataTGTGGTCATTGACTTAATAAATCAAATGATGAATATTTCATAAATGAAATTCTATGTAATGATTAATAATCATCAGAAATATATCAAGTATCTTAATAAAATAAAAATTTATAATtaaataaa                                >  1:259031/1‑128 (MQ=255)
                                                                                               tatGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAATTATAGCAAGTATCTTAATAAGATAAAAATTTATAAGTAAATAAATGa                             >  1:1365497/1‑128 (MQ=255)
                                                                                               tatGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGa                             >  1:2844902/1‑128 (MQ=255)
                                                                                               tatGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGa                             >  2:2864963/1‑128 (MQ=255)
                                                                                                atGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTATTGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGAt                            >  1:1000271/1‑128 (MQ=255)
                                                                                                atGTGGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGAt                            >  2:2139309/1‑128 (MQ=255)
                                                                                                 tgtgGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATc                           <  2:1304266/128‑1 (MQ=255)
                                                                                                 tgtgGTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATc                           <  2:4467034/128‑1 (MQ=255)
                                                                                                  gtgGGCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCa                          <  2:4416994/128‑1 (MQ=255)
                                                                                                     gTCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAAt                       <  2:870524/128‑1 (MQ=255)
                                                                                                      tttttGTATTAATATATTAAATGATGAATATTTAATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATATATGATCAAATc                      <  1:1618143/125‑1 (MQ=255)
                                                                                                      tCATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATc                      <  2:666438/128‑1 (MQ=255)
                                                                                                       gaTTGTCTTAATATATAAAATGATGAATTTTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCt                     <  2:2935010/127‑1 (MQ=255)
                                                                                                       cATTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCt                     <  1:597237/128‑1 (MQ=255)
                                                                                                        aTTGTCTTTATAAATCAAATGATGAATATTTCATTACTGAAAATCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTa                    <  1:309194/128‑1 (MQ=255)
                                                                                                        aTTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAATTATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTa                    >  1:2429765/1‑128 (MQ=255)
                                                                                                        aTTGTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTa                    <  2:1237084/128‑1 (MQ=255)
                                                                                                        aTTGTCTTAATAAATCAAATGATGAATATATCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTa                    <  2:1866286/128‑1 (MQ=255)
                                                                                                           gTCTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACt                 <  2:591328/128‑1 (MQ=255)
                                                                                                             cTTAATAAATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACttt               >  1:231471/1‑128 (MQ=255)
                                                                                                                aataaATCAAATGATGAATCTTTCATTACTGAAATTCTAAGTAATGAAAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTatca            <  1:946783/128‑2 (MQ=255)
                                                                                                                 ataaATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTttctt           <  2:1089164/128‑1 (MQ=255)
                                                                                                                 ataaATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTttctt           >  2:4067426/1‑128 (MQ=255)
                                                                                                                  taaATCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTttcttc          <  2:3094149/128‑1 (MQ=255)
                                                                                                                   aaaTCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTtcttct         <  2:277464/128‑1 (MQ=255)
                                                                                                                   aaaTCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTtcttct         >  1:4215918/1‑128 (MQ=255)
                                                                                                                    aaTCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTTCTTCTa        >  2:1988780/1‑128 (MQ=255)
                                                                                                                     aTCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCATATCTAACTTTTCTTCTaa       >  2:2336355/1‑128 (MQ=255)
                                                                                                                     aTCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTTCTTCTaa       <  2:379114/128‑1 (MQ=255)
                                                                                                                      tCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATTATCAAATCTAACTATTCTTCTaaa      >  1:2210116/1‑128 (MQ=255)
                                                                                                                      tCAAATGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTTCTTCTaaa      >  1:3739320/1‑128 (MQ=255)
                                                                                                                         aaTGATGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTTCTTCTAAATGt   <  1:514680/128‑1 (MQ=255)
                                                                                                                          atgatgAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTTCTTCTAAATGtt  >  2:1699343/1‑128 (MQ=255)
                                                                                                                          atgatgAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTTCTTCTAAATGtt  >  1:20277/1‑128 (MQ=255)
                                                                                                                          atgatgAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAAAGATCAAATCTAACTATTCTTCTAAATGtt  <  1:845485/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                            
ATCTAAAAACTTATAACAAGTTTGATAATAATGAGCATAAGTTAATTGATCACTAAGAAGTTTATATTTATTAATTTGTCTAAAATAATTAATAATATGTGGTCATTGTCTTAATAAATCAAATGCTGAATATTTCATTACTGAAATTCTATGTAATGATAAATAATCATCAGAAATATAGCAAGTATCTGAATAAGATAAAAATTTATAAGTAAATAAATGATCAAATCTAACTTTTCTTCTAAATGTT  >  CP016816/436932‑437181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: