Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 516,362 T→C intergenic (+193/+313) JCVSYN2_02375 → / ← JCVSYN2_02380 antitermination protein NusG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816516,3620TC98.8% 8735.0 / NA 2424intergenic (+193/+313)JCVSYN2_02375/JCVSYN2_02380antitermination protein NusG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (1103/1286);  minor base A (19/10);  total (1122/1302)
Fisher's exact test for biased strand distribution p-value = 4.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2446 total aligned reads displayed.

AAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAA  >  CP016816/516237‑516486
                                                                                                                             |                                                                                                                             
aaaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAacat                                                                                                                             <  1:1941825/128‑1 (MQ=255)
    aTAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAAAATTTTa                                                                                                                         >  2:3334709/1‑128 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAATAAACATTTTAg                                                                                                                        >  1:3993336/1‑128 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAg                                                                                                                        >  1:772273/1‑128 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAg                                                                                                                        <  1:175662/128‑1 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAg                                                                                                                        >  1:1456062/1‑128 (MQ=255)
       aaaTATATTTTTAAAAAAAATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTa                                                                                                                      <  1:425277/128‑1 (MQ=255)
        aaaatttttttAAAAAATATTTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAt                                                                                                                     <  1:3102824/122‑1 (MQ=255)
        aaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTTAAATTTTAATAAAAAAACATTTTAGTAt                                                                                                                     >  1:4488274/1‑128 (MQ=255)
         atatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAtt                                                                                                                    >  2:3162735/1‑128 (MQ=255)
          tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                   <  2:3661735/128‑1 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                                  >  1:3185858/1‑128 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                                  <  2:1538787/128‑1 (MQ=255)
             atTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                   <  2:4354522/125‑1 (MQ=255)
                tttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAtt                                                                                                             <  2:2912979/128‑1 (MQ=255)
                  tAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtat                                                                                                           >  1:4340495/1‑128 (MQ=255)
                   aaaaaaTATTTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtat‑a                                                                                                         <  1:2175132/128‑1 (MQ=255)
                   aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtat‑a                                                                                                         >  2:4503680/1‑128 (MQ=255)
                   aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTCTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtat‑a                                                                                                         >  2:208223/1‑128 (MQ=255)
                     aaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑aaa                                                                                                       >  1:4108374/1‑128 (MQ=255)
                       aaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTAAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTAGCAATTAT‑ataaa                                                                                                     >  1:2613274/1‑128 (MQ=255)
                        atatGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAATGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑aaaaaa                                                                                                    <  2:1875549/128‑1 (MQ=255)
                         tatGTTTTTATAAGATTAGAAAAAAAGATTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAg                                                                                                   <  2:1654927/128‑1 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGt                                                                                                  <  1:2356577/128‑1 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGt                                                                                                  <  2:1778370/128‑1 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGt                                                                                                  <  1:3465633/128‑1 (MQ=255)
                          atGTTTTTAGAATATTAGAAAATAAGCTTATTTTAAGGGCTTTTTTAAATATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGt                                                                                                  <  1:2098317/128‑1 (MQ=255)
                              ttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTAt                                                                                              >  2:348613/1‑128 (MQ=255)
                              ttttATAAGAGAAGAAAATAAGCATATATTAAGGGCTTTTTAAAATATTATTTAAAAATTTGATTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTAt                                                                                              <  1:1370135/128‑1 (MQ=255)
                                 aatcaGATAAGAATAAAACGATATACTAAGGGCAATTTTAAAGATTATTTAAAATTTTGTTGTTATATTAAATTAAAAAGTTAATATAATATCATTTAAGGATTTGCACTTAT‑AAAATTGTTTATGat                                                                                           <  1:2456752/124‑1 (MQ=255)
                                   taAGATTAGAAAATAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGataa                                                                                         <  1:4402329/128‑1 (MQ=255)
                                   taAGATTAGAAAAACAGCTTATATTAAGGGCTTTTTTAAAGATTATTTAAAAATTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGataa                                                                                         <  1:1314949/128‑1 (MQ=255)
                                   taAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGataa                                                                                         <  1:957305/128‑1 (MQ=255)
                                     aGATTAGAAAAAAAGCTTATTTTAAGTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGataata                                                                                       <  2:4047666/128‑1 (MQ=255)
                                     aGATTAGAAAAAAAGATTATTTTAAGGGCTTTTTTAAAGATTATTT‑AAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATATGCAATTATAAAAAAAGTTTATGataata                                                                                       <  1:850380/128‑1 (MQ=255)
                                       aTTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAAtata                                                                                     <  2:1270983/128‑1 (MQ=255)
                                        ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATAc                                                                                    <  2:3586780/128‑1 (MQ=255)
                                            aaaataaGCTTATTTTAATTGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATATTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTAt                                                                                <  2:1129596/128‑1 (MQ=255)
                                             aaaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTAtt                                                                               <  1:3649873/128‑1 (MQ=255)
                                                aaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTATAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAat                                                                            <  1:1148080/128‑1 (MQ=255)
                                                aaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAat                                                                            >  1:562103/1‑128 (MQ=255)
                                                  aGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAatat                                                                          >  2:1027885/1‑128 (MQ=255)
                                                    cTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAg                                                                        <  1:1562981/128‑1 (MQ=255)
                                                     ttATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGa                                                                       >  1:3539121/1‑128 (MQ=255)
                                                      tATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAAAATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGaa                                                                      >  2:2328091/1‑128 (MQ=255)
                                                       aTTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                                     >  1:2017043/1‑128 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                                   >  2:1965450/1‑128 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                                   <  2:1447980/128‑1 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACATATTAATATAGAATaa                                                                   >  1:339812/1‑128 (MQ=255)
                                                          ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑ATAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                  >  1:3917961/1‑128 (MQ=255)
                                                          ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                  >  1:4183656/1‑128 (MQ=255)
                                                           tAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAaac                                                                 >  1:1667705/1‑128 (MQ=255)
                                                              gggCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaac                                                              <  2:4563278/128‑1 (MQ=255)
                                                                gCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTAATAATATAGAATAAACAACTa                                                            <  2:2618216/128‑1 (MQ=255)
                                                                  ttttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGa                                                          <  2:663425/128‑1 (MQ=255)
                                                                   tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         <  2:3853321/128‑1 (MQ=255)
                                                                   tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         >  2:1364640/1‑128 (MQ=255)
                                                                   tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         >  1:1221255/1‑128 (MQ=255)
                                                                    ttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaa                                                        <  2:2522098/128‑1 (MQ=255)
                                                                     tttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaaa                                                       <  2:831600/128‑1 (MQ=255)
                                                                      ttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaaa                                                       <  2:576109/127‑1 (MQ=255)
                                                                       tAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAaga                                                     <  2:2386602/128‑1 (MQ=255)
                                                                        aaaGATTATTTAAAATTTTGTTTTTATTTATAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAagag                                                    <  1:3021303/128‑1 (MQ=255)
                                                                          aGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGaa                                                  <  1:1084623/128‑1 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  2:2206525/1‑128 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  2:1208086/1‑128 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  2:2456860/1‑128 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  1:3818302/1‑128 (MQ=255)
                                                                                 ttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATAAATATATAATAAACAACTAGAAAAGAGAAAtattat                                           >  2:225988/1‑128 (MQ=255)
                                                                                  tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                          >  2:277242/1‑128 (MQ=255)
                                                                                     aaTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGt                                       >  1:2268775/1‑128 (MQ=255)
                                                                                      aTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTa                                      >  2:3111330/1‑128 (MQ=255)
                                                                                       ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATTCCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                                     <  1:1000225/128‑1 (MQ=255)
                                                                                       ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                                     <  1:1850933/128‑1 (MQ=255)
                                                                                        tttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATg                                    <  1:3745106/128‑1 (MQ=255)
                                                                                         ttGATTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTTCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTGGTATGa                                   <  1:470240/128‑1 (MQ=255)
                                                                                          tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                                  >  2:926647/1‑128 (MQ=255)
                                                                                          tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                                  <  1:2867539/128‑1 (MQ=255)
                                                                                             ttttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGc                               <  2:441849/128‑1 (MQ=255)
                                                                                               ttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTTCAATTAT‑AAAAAAGTTTATTATAATATACCTATTAATATATAATAAACAACTATAATAGAGAAATATTATTAGTATGATAGCGa                             >  2:4163094/1‑128 (MQ=255)
                                                                                               ttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGa                             >  2:3399443/1‑128 (MQ=255)
                                                                                                   ttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGATTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaatta                         <  1:695738/128‑1 (MQ=255)
                                                                                                    tttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaa                        <  1:2716730/128‑1 (MQ=255)
                                                                                                      taattaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaatt                      >  2:2026615/1‑128 (MQ=255)
                                                                                                       aattaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttt                     >  2:481740/1‑128 (MQ=255)
                                                                                                        attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaattat                    <  2:2847972/128‑3 (MQ=255)
                                                                                                        attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                    <  1:2550958/128‑1 (MQ=255)
                                                                                                        attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                    <  1:2793526/128‑1 (MQ=255)
                                                                                                        attaaAATTTTAATAAAAAAACATTTTAGTATTTGAAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                    <  1:3334709/128‑1 (MQ=255)
                                                                                                         ttaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttttt                   <  2:270344/128‑1 (MQ=255)
                                                                                                          taaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTa                  <  1:327169/128‑1 (MQ=255)
                                                                                                            aaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGATTAATCAACTAGAAAAGAGAAATATTATTAGTATGATAGCGATTTTATTTTTaaa                >  2:4271321/1‑128 (MQ=255)
                                                                                                            aaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaa                <  2:3024137/128‑1 (MQ=255)
                                                                                                             aaTTTTAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa               >  2:3953961/1‑128 (MQ=255)
                                                                                                                tttAATAAAAAAACATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAattt            >  2:3248509/1‑128 (MQ=255)
                                                                                                                   aataaaaaaaCATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAatttatt         <  2:738962/123‑1 (MQ=255)
                                                                                                                     taaaaaaaCATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt       <  2:3735011/125‑1 (MQ=255)
                                                                                                                     taaaaaaaCATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt       <  1:62857/125‑1 (MQ=255)
                                                                                                                     taaaaaaaCATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt       <  1:4562337/125‑1 (MQ=255)
                                                                                                                          aaaCATTTTAGTATTTGCAAATAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaataa  <  1:2948874/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                             
AAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTAT‑AAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAA  >  CP016816/516237‑516486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑