Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 113,623 G→A R244K (AGA→AAA)  JCVSYN2_00455 → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816113,6230GA99.2% 6795.8 / NA 2160R244K (AGA→AAA) JCVSYN2_00455ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1014/1124);  minor base T (12/6);  total (1029/1131)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2203 total aligned reads displayed.

AAGTGTTAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAGAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCTAGTCAT  >  CP016816/113498‑113747
                                                                                                                             |                                                                                                                            
 aGTGTTAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAaaaa                                                                                                                           >  2:402110/1‑128 (MQ=255)
  gtgtTAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAat                                                                                                                          >  2:1619957/1‑128 (MQ=255)
   tgtTAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAatg                                                                                                                         >  2:678618/1‑128 (MQ=255)
   tgtTAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAatg                                                                                                                         >  2:350000/1‑128 (MQ=255)
    gtTAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAatga                                                                                                                        <  1:1002176/128‑1 (MQ=255)
     ttAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAatgat                                                                                                                       >  2:3040679/1‑128 (MQ=255)
     ttAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAatgat                                                                                                                       <  2:168784/128‑1 (MQ=255)
      tAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCTTGATGCAATTTTAAAAGTTAAAGATGTATAAAATACTATTTAAAATACTTTCCAAGATCTGCTAGCTTTTTAAAatgtag                                                                                                                      >  1:2080466/1‑125 (MQ=255)
           tAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAAt                                                                                                                 <  1:3371212/128‑1 (MQ=255)
            aGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATg                                                                                                                >  2:3239323/1‑128 (MQ=255)
             gAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGa                                                                                                               <  2:1542253/128‑1 (MQ=255)
             gAATTACAAGATGCAATTAACAAATATCAAGACTTTGATCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGa                                                                                                               <  2:3630418/128‑1 (MQ=255)
              aaTTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGttaattaa                                                                                                              >  2:1977229/1‑128 (MQ=255)
               aTTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAg                                                                                                             <  1:573366/128‑1 (MQ=255)
                 tACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGt                                                                                                           >  1:3914311/1‑128 (MQ=255)
                    aaGATGCATTTAAGAAATATCAAGACTTAGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACATTACAAGAAATGCTAGCTATTAAAAATGATGTTACTGAAGTAAat                                                                                                       <  1:1641171/128‑1 (MQ=255)
                        tGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTACTGAAgtatatttac                                                                                                    >  2:1415102/1‑119 (MQ=255)
                         gCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATAc                                                                                                   >  1:2170429/1‑128 (MQ=255)
                         gCAATTAACAAATATCAAGACTTTGTACATGATTCAATTTAAAAAGTTAAAGATTTAGAACATACTAGTTATAATACTTAAGAAGAAATGATAGCTATAAATTAAGATGTAAATCAAGGTCAAGATAc                                                                                                   >  1:2822685/1‑128 (MQ=255)
                            aTTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTc                                                                                                >  2:2747052/1‑128 (MQ=255)
                             ttAACAAATCTCAAGACTTTGTTCATGATTCTATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCa                                                                                               <  1:816051/128‑1 (MQ=255)
                             ttAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTATTGAAGGTAAATATACTTCa                                                                                               <  2:2677691/128‑1 (MQ=255)
                             ttAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCa                                                                                               <  2:4330853/128‑1 (MQ=255)
                             ttAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCa                                                                                               <  1:1080243/128‑1 (MQ=255)
                              tAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAg                                                                                              <  1:3071824/128‑1 (MQ=255)
                              tAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAg                                                                                              <  2:2201363/128‑1 (MQ=255)
                              tAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAg                                                                                              <  1:3681987/128‑1 (MQ=255)
                                 cAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTaa                                                                                           >  2:508198/1‑128 (MQ=255)
                                 cAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTAAATGAAGGTAAATATACTTCAGTaa                                                                                           >  2:857975/1‑128 (MQ=255)
                                     tatCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTATCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTaa                                                                                       >  2:588041/1‑128 (MQ=255)
                                     tatCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTaa                                                                                       <  1:2008343/128‑1 (MQ=255)
                                      atCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAg                                                                                      >  1:4172882/1‑128 (MQ=255)
                                            aCTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGtttttt                                                                                <  1:4407687/128‑1 (MQ=255)
                                              tttGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGa                                                                              >  1:2528466/1‑128 (MQ=255)
                                               ttGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGAt                                                                             <  1:2979354/128‑1 (MQ=255)
                                                tGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTATCTAAGTTTTTTGATc                                                                            >  1:661044/1‑128 (MQ=255)
                                                tGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGAGGTTAAGGAAGGTAAATATTCTTCAGTAACTAAGTTTTTTGATc                                                                            >  1:1932652/1‑128 (MQ=255)
                                                tGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACAATTTATAATACTTTACATGAAATGCTAACTATTAAAAATGATCTTAATGAATCTATATATACTTCAGTACCTAAGattttttaga                                                                            >  1:2312944/1‑124 (MQ=255)
                                                      tGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTTTTAAAAATGATGTTAATGAAGGTAATTATTCTTCAGTAACTAAGTTTTTTGATCAAATgg                                                                      >  1:900499/1‑128 (MQ=255)
                                                      tGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTGAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATgg                                                                      >  1:1526294/1‑128 (MQ=255)
                                                       gATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATggg                                                                     >  1:4114991/1‑128 (MQ=255)
                                                       gATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATggg                                                                     <  1:2747052/128‑1 (MQ=255)
                                                         ttCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTATAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGtt                                                                   <  1:1464483/128‑1 (MQ=255)
                                                          tCAATTTTAATAGTTAAAGATTGAGAAAATACTATTTATAATTCTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTc                                                                  <  2:2298630/128‑1 (MQ=255)
                                                            aaTTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTa                                                                >  1:1303869/1‑128 (MQ=255)
                                                                ttAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGAtt                                                            <  2:3150934/128‑1 (MQ=255)
                                                                 tAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTa                                                           >  1:72208/1‑128 (MQ=255)
                                                                   aaaGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTaaa                                                         >  1:2631597/1‑128 (MQ=255)
                                                                   aaaGTTAAAGATTTAGAAAATACTATATATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTaaa                                                         <  1:2896039/128‑1 (MQ=255)
                                                                     aGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAAt                                                       <  2:1128451/128‑1 (MQ=255)
                                                                      gTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAAtt                                                      <  1:1240380/128‑1 (MQ=255)
                                                                      gTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAAtt                                                      <  2:1332597/128‑1 (MQ=255)
                                                                        tAAAGATTTAGAAAATACTATTTATAGTACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGCTTTAAATTAg                                                    >  1:1743248/1‑128 (MQ=255)
                                                                        tAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAg                                                    >  2:4128085/1‑128 (MQ=255)
                                                                         aaaGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGt                                                   <  2:4198883/128‑1 (MQ=255)
                                                                         aaaGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGt                                                   >  1:3457342/1‑128 (MQ=255)
                                                                          aaGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGtt                                                  >  1:3551797/1‑128 (MQ=255)
                                                                          aaGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTATTTGATCAAATGGGTTCTAGATTAAAATTAGtt                                                  >  1:3807227/1‑128 (MQ=255)
                                                                           aGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGtta                                                 >  2:4434022/1‑128 (MQ=255)
                                                                           aGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGtta                                                 <  1:316885/128‑1 (MQ=255)
                                                                           aGATATAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGtta                                                 <  2:371820/128‑1 (MQ=255)
                                                                             aTTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGttatt                                               <  1:1137538/128‑1 (MQ=255)
                                                                              tttAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTATATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGttatta                                              >  1:4613909/1‑128 (MQ=255)
                                                                               ttAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTaa                                             <  2:247619/128‑1 (MQ=255)
                                                                               ttAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTaa                                             <  2:4257868/128‑1 (MQ=255)
                                                                               ttAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTaa                                             <  1:4504542/128‑1 (MQ=255)
                                                                                tAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTaaa                                            >  2:122666/1‑128 (MQ=255)
                                                                                  gAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAg                                          >  2:3867499/1‑128 (MQ=255)
                                                                                     aaTACTATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAATGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCa                                       <  2:767798/128‑1 (MQ=255)
                                                                                          tATTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAAc                                  <  2:3956857/128‑1 (MQ=255)
                                                                                          tATTTATAATACTTTAACTGAAATGATATTTATTTAAATTGATGTTAATGATGGTAAATATACTTCTGTAACTAAGTTTTTTTATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAAc                                  <  1:2241738/128‑1 (MQ=255)
                                                                                           aTTTATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTATATTAAAATTAGTTATTAAAAGTCAAAAACt                                 >  1:425155/1‑128 (MQ=255)
                                                                                             ttATAATACTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTaa                               <  2:3399382/128‑1 (MQ=255)
                                                                                                taataCTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTTAttt                            >  1:2386258/1‑127 (MQ=255)
                                                                                                taataCTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTa                            >  1:1844943/1‑128 (MQ=255)
                                                                                                   taCTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTActc                         >  2:180744/1‑128 (MQ=255)
                                                                                                    aCTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTActcc                        <  2:919387/128‑1 (MQ=255)
                                                                                                    aCTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTActcc                        <  1:972673/128‑1 (MQ=255)
                                                                                                    aCTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTActcc                        <  2:1887435/128‑1 (MQ=255)
                                                                                                     cTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTActcct                       <  1:3623802/128‑1 (MQ=255)
                                                                                                     cTTTACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTActcct                       <  2:2049093/128‑1 (MQ=255)
                                                                                                       ttACAAGAACTGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCTGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCa                     <  2:2409133/128‑1 (MQ=255)
                                                                                                       ttACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCa                     <  1:2454796/128‑1 (MQ=255)
                                                                                                        tACAAGAAATGCTAGCTATTAAAAATGATGTTAATGAATGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCaa                    <  1:492336/128‑1 (MQ=255)
                                                                                                          cAAGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACt                  >  1:1399444/1‑128 (MQ=255)
                                                                                                            aGAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTg                <  2:1051970/128‑1 (MQ=255)
                                                                                                             gAAATGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGa               >  2:1275920/1‑128 (MQ=255)
                                                                                                              aaaTGCTAGCTATTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGaa              <  2:148427/128‑1 (MQ=255)
                                                                                                               aaTGCTTGCTATTAAATATGATGGTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAATATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAg             <  2:2929393/128‑1 (MQ=255)
                                                                                                                   ctagctaTTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGc         <  1:4007367/128‑1 (MQ=255)
                                                                                                                    tagctaTTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCt        <  2:3551797/128‑1 (MQ=255)
                                                                                                                    tagctaTTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCt        <  1:3292865/128‑1 (MQ=255)
                                                                                                                    tagctaTTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCt        <  2:2828190/128‑1 (MQ=255)
                                                                                                                      gctaTTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGTGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTAAACATCAACTTGAatataatag      >  2:4639570/1‑123 (MQ=255)
                                                                                                                      gctaTTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCTAg      >  2:2126459/1‑128 (MQ=255)
                                                                                                                       ctaTTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCTAGt     <  2:3799533/128‑1 (MQ=255)
                                                                                                                         aTTAAAATTGATGGTAATGAAGTTAAATATACTTCAGTAACTATGTTTTTTGATCAAATGGTTTCTAGATTATAATTAGTTATTAACTGTCTAAAACTAATTACTCCTCTCCTTTAAGATGCTAGTCa   <  1:4330043/128‑1 (MQ=255)
                                                                                                                         aTTAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCTAGTCa   >  2:4530301/1‑128 (MQ=255)
                                                                                                                          ttAAAAATGATTTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATTTGTTATAGATTAAAATTAGTTATTTAAAGTCAAAAACTAATTACTACTCAACTTGAAGATGATAGTCAt  >  2:2601858/1‑128 (MQ=255)
                                                                                                                          ttAAAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCTAGTCAt  >  2:3310445/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
AAGTGTTAAAGTAGAATTACAAGATGCAATTAACAAATATCAAGACTTTGTTCATGATTCAATTTTAAAAGTTAAAGATTTAGAAAATACTATTTATAATACTTTACAAGAAATGCTAGCTATTAGAAATGATGTTAATGAAGGTAAATATACTTCAGTAACTAAGTTTTTTGATCAAATGGGTTCTAGATTAAAATTAGTTATTAAAAGTCAAAAACTAATTACTCCTCAACTTGAAGATGCTAGTCAT  >  CP016816/113498‑113747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: