BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.6%	5310.7 / NA	1683	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/4); new base T (911/763); total (914/769)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.6%	5213.0 / NA	1684	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/21); new base T (912/748); total (914/770)
Fisher's exact test for biased strand distribution p-value = 5.80e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1755 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420031‑420281

||

    taaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatt                                                                                                                            >  1:3708151/1‑128 (MQ=255)

    taaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatt                                                                                                                            >  1:1656601/1‑128 (MQ=255)

     aaattCATTCATACTTTTAGTCTCATAAATCTAAGTATCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattt                                                                                                                           <  1:2470852/128‑2 (MQ=255)

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                         >  2:1724630/1‑125 (MQ=255)

        ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                        <  2:1183466/128‑5 (MQ=255)

          cattcatAATTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                      <  1:3178371/128‑1 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    >  2:3743064/1‑128 (MQ=255)

              catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                  <  2:2327564/128‑1 (MQ=255)

                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                <  1:756331/128‑1 (MQ=255)

                  cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                              <  1:3961019/128‑1 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                             >  2:511718/1‑128 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACATTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                             <  1:2197050/128‑1 (MQ=255)

                        gTCTCATAAATCTAAGTATCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttttt                                                                                                        <  1:546978/128‑1 (MQ=255)

                         tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                       <  2:316372/128‑1 (MQ=255)

                          ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTTATATATTAGAAAACTAATAGCACTATTTTAAAAAATTTTTAATTTTAATAACTAAATTATTTTTCa                                                                                                      >  2:2268055/1‑128 (MQ=255)

                            cATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                    <  1:1966622/128‑1 (MQ=255)

                            cATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     >  2:1566689/1‑127 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   >  1:998862/1‑128 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   >  2:559192/1‑128 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   <  2:2645685/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTAATTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   <  1:1487642/128‑1 (MQ=255)

                               aaaTCTAAGTTTCTAAAAATATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                 <  2:1829718/128‑1 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                <  2:2080513/128‑1 (MQ=255)

                                  tCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTATTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                              >  1:3460042/1‑128 (MQ=255)

                                   cTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                             <  1:126343/128‑1 (MQ=255)

                                     aaGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                           <  2:1072619/128‑1 (MQ=255)

                                     aaGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                           <  1:4224300/128‑1 (MQ=255)

                                      aGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                          <  1:4135216/128‑1 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                         >  2:3442707/1‑128 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                         >  2:4422216/1‑128 (MQ=255)

                                        tttCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                        <  1:4554134/128‑1 (MQ=255)

                                        tttCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                        <  2:4499818/128‑1 (MQ=255)

                                         ttCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTTAattatt                                                                                       >  2:871712/1‑128 (MQ=255)

                                         ttCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       >  2:743634/1‑128 (MQ=255)

                                         ttCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       <  2:3588726/128‑1 (MQ=255)

                                         ttCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       >  1:4387443/1‑128 (MQ=255)

                                          tCTAAAATTATTTTATCATTT‑TAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                      <  2:935498/128‑1 (MQ=255)

                                          tCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                      >  2:2715263/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTAtttt                                                                                     >  2:3866968/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  1:3801702/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:2398717/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:4059135/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:413008/1‑128 (MQ=255)

                                            tAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  2:1502677/128‑1 (MQ=255)

                                            tAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAATTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  1:3064044/128‑1 (MQ=255)

                                            tAAAATTA‑TTTATCATTTAAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  1:2680465/128‑1 (MQ=255)

                                                attattTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                <  1:4640761/128‑1 (MQ=255)

                                                  tattTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAAATACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                              <  2:218478/128‑1 (MQ=255)

                                                   attTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                             <  1:1101267/128‑1 (MQ=255)

                                                   attTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGATAAATTATTTTAAAATCAt                                                                             >  2:3523779/1‑128 (MQ=255)

                                                     tttATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                           <  1:627225/128‑1 (MQ=255)

                                                     tttATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                           >  1:2578204/1‑128 (MQ=255)

                                                      ttATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                          <  2:2589390/128‑1 (MQ=255)

                                                      ttATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                          >  1:1385493/1‑128 (MQ=255)

                                                      ttATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                          <  2:1424591/128‑1 (MQ=255)

                                                      ttATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTATTAATTTTAATAACTGAATTATTTTTCAAATTGATAAATTATTTTCAAATCATTAt                                                                          >  1:2291992/1‑128 (MQ=255)

                                                        aTCATTT‑ATAAAAATAATACTTATAATAGTTGATATATTAGAAAACTACTAGCACTTTATTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                        <  1:4068897/128‑1 (MQ=255)

                                                        aTCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                        <  2:2847617/128‑1 (MQ=255)

                                                          cATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                      <  1:2820789/128‑1 (MQ=255)

                                                             tt‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                   <  1:1004597/128‑1 (MQ=255)

                                                                   aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                              >  1:4300689/1‑128 (MQ=255)

                                                                   aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                              >  2:4186660/1‑128 (MQ=255)

                                                                     aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                            <  2:3065729/128‑1 (MQ=255)

                                                                      ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                           >  2:1914714/1‑128 (MQ=255)

                                                                       taataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTa                                                          >  2:1254643/1‑128 (MQ=255)

                                                                       taataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTa                                                          >  2:299592/1‑128 (MQ=255)

                                                                              tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATATCTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTTTCTATTAATCAATATTAT‑‑GCTCG‑t                                                >  1:2932891/1‑128 (MQ=255)

                                                                              tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTctt                                                 >  1:3640309/1‑126 (MQ=255)

                                                                              tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTctt                                                 >  2:3301622/1‑126 (MQ=255)

                                                                               ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑tt                                               >  2:3823994/1‑128 (MQ=255)

                                                                               ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAATTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑tt                                               >  1:2084659/1‑128 (MQ=255)

                                                                                tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑TATCG‑ttt                                              >  2:3981240/1‑128 (MQ=255)

                                                                                      ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                              <  1:853369/77‑1 (MQ=255)

                                                                                           atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑tttttttttttttt                                   >  2:4356851/1‑128 (MQ=255)

                                                                                               tAGAAAACTACAAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATCAATTAATCAATTTTAT‑‑GCTCG‑ttttttttttttttttac                               <  2:3688342/128‑3 (MQ=255)

                                                                                                 taaaaATAATATCAATTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATATATTAATAAATTTTAT‑‑GCTCG‑tttttttttttttttacaaa                             <  1:3555366/127‑6 (MQ=255)

                                                                                                  aaaaCTACTACCACTTTTTTAAAAAATTTTTAATTTTAAAAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAACAATTAATAAAATTTAT‑‑GCGCG‑TTTTTTTTTTTTTTTTTACaa                            <  2:429581/128‑1 (MQ=255)

                                                                                                    aacaacTATCAATTTTTTTAAAAATTTTTAATATTAATAACTAAAATTTTTTTAAAATTTTAAAATTTTTATCAAAACATTATATATTAATCAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTTACaaa                           <  1:515640/128‑1 (MQ=255)

                                                                                                     aatactaGAAATTTTTTAAAAAATTTTTAATTTTAAAAACTTAATTATTTTTAAAAATTTTAAATTATTTTAAAATAATTATAATTTAATAAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACaaaaa                         <  2:2524395/126‑1 (MQ=255)

                                                                                                      ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTAAAAAAat                        >  2:1127982/1‑128 (MQ=255)

                                                                                                       tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTTaaaaaaat                       >  2:1360040/1‑126 (MQ=255)

                                                                                                       tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAata                       >  1:1166593/1‑128 (MQ=255)

                                                                                                         ctataaattttttAAAAAATTTTTAATTTTAATTACTTAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑‑‑TTTTTTTTTTTTTTTACAAAAATATAat                   <  2:2925032/121‑1 (MQ=255)

                                                                                                         ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCT‑TTTTTTTTTTTTTTTTTACAAAAAtata                     >  1:3515888/1‑128 (MQ=255)

                                                                                                          tttttattttttTAAAATTTTTTTATTTTAATATATTAATTTTTTTTAAAATTTTTATATTTTTTTAAAATTATTATATATTAATAAATTTTTT‑‑TCTTG‑‑TTTTTTTTTTTTTTTTACAAAAATATAat                   <  1:3378360/122‑1 (MQ=255)

                                                                                                          taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTAAAAAAAAATaa                    >  1:3254636/1‑128 (MQ=255)

                                                                                                          taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑‑TTTTTTTTTTTTTTTTACAAAAATATAat                   >  1:1862760/1‑128 (MQ=255)

                                                                                                            taatttttttTAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAAATGTTAAATTTTTTTAAAATCAATATATATTAATTAATTTTAT‑‑GCTCGTTTTTTTTTTTTTTTTTTACAAAAAtatagt                   <  2:2452662/124‑3 (MQ=255)

                                                                                                            gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAAAATAatt                  >  1:175768/1‑128 (MQ=255)

                                                                                                            gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTAAAAAAAAATAatt                  >  1:2389594/1‑128 (MQ=255)

                                                                                                             taattttttAAAAAATTTTTTATTTTTATTAATTAATTATTTTTAAAATTTTTAATTTATTTTAAAATAATTATATATTAATAAATTTTTTTATCTTT‑TTTTTTTTTTTTTTTTTACAAAAATATAat                   <  2:627189/125‑1 (MQ=255)

                                                                                                             cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAattt                 >  1:1588204/1‑128 (MQ=255)

                                                                                                             cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAATAAAattt                 >  2:3244772/1‑128 (MQ=255)

                                                                                                               cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTAAAAAAATATAatttat               >  2:4613439/1‑128 (MQ=255)

                                                                                                                 tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑TCTCGTTTTTTTTTTTTTTTTTTTAAAAAATAAAatttatt              >  1:3001981/1‑128 (MQ=255)

                                                                                                                   tttAAAAAATTTTATATTTAAAAAATTTTATTATTTTTAAATTTGTTAAATTTTTTTCAATACATATACTTTAAATCAATTTTAT‑‑GCTCT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa           <  2:2016038/128‑1 (MQ=255)

                                                                                                                    ttAAAAAATTTTTAATATTTATAAATTTATTTTTTTTAAAATTTTTAAATTATTTTAAAATAAATATTTATTAAATAATTTTTT‑‑GCT‑T‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt         <  2:805711/128‑1 (MQ=255)

                                                                                                                      aaaaaaTTTTTTATTTTAATAAATTTATTTTTTTTTAAATTTTTTAATTTTTTTAAAATTAATAATTTTTAATAAATTTTAT‑‑TGTCGTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt         <  2:1656601/128‑1 (MQ=255)

                                                                                                                       aaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATttaattaa       >  1:3877354/1‑128 (MQ=255)

                                                                                                                         aaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTTTTTTATaaaaa     >  1:4453298/1‑127 (MQ=255)

||

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTT‑AAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAT‑‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420031‑420281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`