Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,163 2 bp→TT intergenic (‑446/+396) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1630AT99.5% 5319.5 / NA 1684intergenic (‑446/+397)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (6/2);  new base T (908/766);  total (914/770)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1640AT98.3% 4836.7 / NA 1675intergenic (‑447/+396)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (7/21);  new base T (902/744);  total (909/766)
Fisher's exact test for biased strand distribution p-value = 1.90e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1689 total aligned reads displayed.

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420041‑420287
                                                                                                                          ||                                                                                                                            
cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTAAATa                                                                                                                          <  1:4337227/128‑1 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTAATAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                        <  1:801235/128‑1 (MQ=255)
   tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                       >  1:1995509/1‑128 (MQ=255)
    tatACTTTTAGTCTCATATATCTAAGTTTCTAATATTATTTTATCATTTAAATAAATTATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                      <  2:3632250/127‑1 (MQ=255)
    catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                      <  2:248319/128‑1 (MQ=255)
       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTATAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACAGAat                                                                                                                   >  2:3036640/1‑128 (MQ=255)
       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                   <  1:3416044/128‑1 (MQ=255)
       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATATTAATAACTGAat                                                                                                                   >  2:1093992/1‑128 (MQ=255)
       aCTTTTAGGCTCATACATCTAAGATTCTAAAAATATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                   <  1:2889464/128‑1 (MQ=255)
         ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                 <  2:230901/128‑1 (MQ=255)
            tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                              <  1:224051/128‑1 (MQ=255)
             aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                <  2:2387912/125‑1 (MQ=255)
             aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                             >  1:4276392/1‑128 (MQ=255)
             aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGAAATATTTTAAAAAATTATTAATTATAATAACTAAATTAtttt                                                                                                             >  2:2701503/1‑128 (MQ=255)
               tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                           >  1:359794/1‑128 (MQ=255)
               tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                           >  1:2307414/1‑128 (MQ=255)
                ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTAGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTAAATAACTGAATTATTATTCa                                                                                                          >  2:807453/1‑128 (MQ=255)
                 tcATATATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATAATAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                         <  1:3742881/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                         <  2:1227513/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTAATTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                         <  2:1471349/128‑1 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                       >  2:559192/1‑128 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                       <  1:3924258/128‑1 (MQ=255)
                    tataTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTTGAAAACTACTAGCAGTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                      >  1:3479957/4‑128 (MQ=255)
                      aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  1:498097/128‑1 (MQ=255)
                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                   >  1:2602619/1‑128 (MQ=255)
                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                     <  1:694861/110‑1 (MQ=255)
                        tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                  <  1:2098237/128‑1 (MQ=255)
                        tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                  <  2:3831509/128‑1 (MQ=255)
                          tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                <  2:4437545/128‑1 (MQ=255)
                           aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATTACTGAATTATTTTTCAATTTGTTAAat                                                                                               >  1:3105137/1‑128 (MQ=255)
                           aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                               <  1:1677141/128‑1 (MQ=255)
                            aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                              <  1:1393495/128‑1 (MQ=255)
                             gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAatta                                                                                             >  2:1692525/1‑128 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  2:2616149/1‑128 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  2:1816249/1‑128 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  2:1379178/1‑128 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  1:1162808/1‑128 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATAATTTTTCAAATTGTTAAattatt                                                                                           >  2:3475624/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                         >  2:638073/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTATCAAATTGTTAAATTAtttt                                                                                         >  2:3378360/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAAAAACTGAATTATTTATCAAAATGTTAAattattat                                                                                         >  2:4613799/1‑126 (MQ=255)
                                 cTAAAATTATATTATCATATAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                         <  1:1791438/128‑1 (MQ=255)
                                     tattattTTATCATTTAAAAATATAATACTTTTTTTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                     <  2:2071387/127‑1 (MQ=255)
                                      attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                    <  1:2740065/128‑1 (MQ=255)
                                       ttattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                   <  1:3007628/128‑1 (MQ=255)
                                           tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                               <  2:4145863/128‑1 (MQ=255)
                                             tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                             <  2:4315623/128‑1 (MQ=255)
                                               tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                           <  2:939437/128‑1 (MQ=255)
                                                cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                          <  1:188352/128‑1 (MQ=255)
                                                cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                          <  2:1022087/128‑1 (MQ=255)
                                                cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           <  2:1532400/111‑1 (MQ=255)
                                                 aatataaaaaaTAATACTTTAATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         <  2:2960250/123‑1 (MQ=255)
                                                 aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATATCTGAATTATTGTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         >  1:4600604/1‑128 (MQ=255)
                                                   ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                       <  1:1004597/128‑1 (MQ=255)
                                                   ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                       >  1:4492196/1‑128 (MQ=255)
                                                   ttAAAAAAATAATACTTTTATTAGATGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                       <  2:4247190/128‑1 (MQ=255)
                                                    tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                      >  2:2805379/1‑128 (MQ=255)
                                                      aaaaaaTAATACTTTTATTATTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                    <  2:3264914/128‑1 (MQ=255)
                                                      aaaaaaTAATACTTTTATTAGTTGATATATTAGAATACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                    <  2:3553657/128‑1 (MQ=255)
                                                       aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                   <  1:4095605/128‑1 (MQ=255)
                                                       aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                   <  2:4374824/128‑1 (MQ=255)
                                                        aatataataCTTTTATTAGTTGATATATTAGAAATCTACTAGCACTTTTTTAAATAATTTTAAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATATTCAAATCATAATCTAAAAATCAAt                                                                  <  1:3529266/125‑1 (MQ=255)
                                                        aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                                  >  1:2398482/1‑128 (MQ=255)
                                                         aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                 <  1:1493639/128‑1 (MQ=255)
                                                          aataatACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                <  2:1298976/128‑1 (MQ=255)
                                                          aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                     <  2:2159278/107‑1 (MQ=255)
                                                          aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                <  2:115385/128‑1 (MQ=255)
                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                               >  1:526630/1‑128 (MQ=255)
                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                               <  2:4079915/128‑1 (MQ=255)
                                                               taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                           >  2:2559663/1‑128 (MQ=255)
                                                                 cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTc                                                         >  1:1100942/1‑128 (MQ=255)
                                                                 cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTc                                                         >  2:1601912/1‑128 (MQ=255)
                                                                  ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                        <  1:4418904/64‑1 (MQ=255)
                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGt                                                      >  2:3913083/1‑128 (MQ=255)
                                                                    ttttttttttatatatAATAAAACTAATATAAATTTTTTAAAAAATTTTTAATTTTAAAAAATTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATTTTATGCTCGtt                                                     <  2:87539/118‑1 (MQ=255)
                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttt                                                    >  1:2502474/1‑128 (MQ=255)
                                                                      attaGTTGATATATTAGAAAACTACTAGCTCTTTTTTAAAAAATTTTTAATTTTAATAACTTAAATATTTTTTAAATTGTTAAATTATTTTCAAATAATTATCTATTAATCAATTTTATGCTCGtttt                                                   <  1:585727/128‑1 (MQ=255)
                                                                             gATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCGttttttttttt                                            >  1:4173053/1‑128 (MQ=255)
                                                                               tatatTTGAAAAGTAATAGCACTTTTTTAAAATATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttttttttttttt                                          <  1:3292246/128‑1 (MQ=255)
                                                                                atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttt                                         >  2:3783828/1‑128 (MQ=255)
                                                                                      gAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                            >  2:4504622/1‑72 (MQ=255)
                                                                                       aaaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttttttttttttttacaaaaa                                  >  2:511286/1‑121 (MQ=255)
                                                                                        aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttttttttttttttttttacaa                                 >  1:3845546/1‑123 (MQ=255)
                                                                                         aaataatataatttttttAAAAAAATTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTTTTTTAAAATAAATAAATATTAAAAAATTTTATGCTCGTTTTTTTTTTTTTTTTTTACaaa                                 <  2:1877172/116‑1 (MQ=255)
                                                                                         aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATTTTATGATCGTTTTTTTATTTTTTTTAACaaaa                                >  1:2951171/1‑128 (MQ=255)
                                                                                             aatataaattttttAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTTAATTATTTTAAAATAATTATATATTAATAAATTTTATTCTCGTTTTTTTTTTTTTTTTACAAAAAtata                           <  2:322227/120‑1 (MQ=255)
                                                                                               taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCTTTTTTTTTTTTTTTTTTAAAAAAATaaaa                          >  2:225055/1‑128 (MQ=255)
                                                                                                aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAAaataat                         >  2:199518/1‑128 (MQ=255)
                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTACAAAAATATAatat                       >  1:3208436/1‑126 (MQ=255)
                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTAAAAAAAATATAtttt                       >  1:941352/1‑128 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAATAATATAatt                        >  1:4018692/1‑128 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTACAAAAATATAattta                      >  1:1591351/1‑128 (MQ=255)
                                                                                                    cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAATATAattta                      >  2:3988652/1‑128 (MQ=255)
                                                                                                    cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAatttat                     >  1:3668980/1‑128 (MQ=255)
                                                                                                     ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                               >  2:3105376/1‑86 (MQ=255)
                                                                                                       ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAatttattta                  >  2:3712103/1‑127 (MQ=255)
                                                                                                       ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAAAATAatttatttt                  >  1:1272701/1‑127 (MQ=255)
                                                                                                        tttAAAAAATTTTTAATTTTAATAAATTAATTTTTTTTTAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTATTATATTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa                 <  2:746632/128‑1 (MQ=255)
                                                                                                           aaaaaaTTTTTAATTTTAATAAATTAAATTTTTTTTAAATTTTTAAATTATTTTAAAATAATTTTATATTAATAAATTTTATTCTCGTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt               <  2:2440328/128‑1 (MQ=255)
                                                                                                              aaattttaaattttTATAACTGAATTTTTTTTAAAATTGGTAAATTTATTTAAAAACATTATATATTTATAAATTTTATGCTTGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAAc           <  2:2889009/128‑1 (MQ=255)
                                                                                                                          ||                                                                                                                            
CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420041‑420287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: