Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 478,738 C→A G301V (GGT→GTT)  JCVSYN2_02210 ← DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,7380CA99.3% 7635.3 / NA 2591G301V (GGT→GTT) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (16/2);  new base A (1294/1266);  total (1310/1281)
Fisher's exact test for biased strand distribution p-value = 1.30e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2658 total aligned reads displayed.

GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAA  >  CP016816/478613‑478862
                                                                                                                             |                                                                                                                            
   aCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAacacca                                                                                                                         >  1:948071/1‑128 (MQ=255)
    cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                        <  2:5722963/128‑1 (MQ=255)
    cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                        >  1:6411834/1‑128 (MQ=255)
    cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACGTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATATCATAACTAGTAAATCTAacaccaa                                                                                                                        >  1:3460018/1‑127 (MQ=255)
       aCGTTTTTAATTATTTACAATAATTGATTTTGCACACATTTATTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTa                                                                                                                     <  2:5504628/128‑1 (MQ=255)
              tCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTTATAATGATTCTTCTATATAAATCATTAATATCAGAAGTAGAAAATCTAACACCATCTAATTTaat                                                                                                              >  1:6077944/1‑128 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                             >  2:6402347/1‑128 (MQ=255)
                attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                            <  1:5941989/128‑1 (MQ=255)
                attattTACAATAATTGATGGGTAACCCATTTCTTTAACTTTTTTTAATCATTCATTTATAATAATGATTTTTCTATATAAATAATTAATTTCATAAGTACTAAATCTAAAACCATCTAATTGaataa                                                                                                            <  2:6131546/128‑1 (MQ=255)
                 ttattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataat                                                                                                           <  1:1666061/128‑1 (MQ=255)
                  tattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                          <  1:829871/128‑1 (MQ=255)
                    tttACTATATTTGATGTTGCACCCATTTCTTTAAATTTTTTTTATCTTTCATTTCTAATAATGATTCTTCTATATATATCATTTATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                        <  1:2571650/128‑1 (MQ=255)
                    tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                        <  2:1634706/112‑1 (MQ=255)
                      tACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGAc                                                                                                      <  2:15653/128‑1 (MQ=255)
                       aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGagg                                                                                                     >  1:3962795/1‑126 (MQ=255)
                       aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATAATTAATTTCAGAAGTAGTAAATCTAACTCCATCTAATTGAATAATTGGACg                                                                                                     >  2:4259047/1‑128 (MQ=255)
                        cAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGa                                                                                                    >  1:2291182/1‑128 (MQ=255)
                         aataatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCATAAGTAGTAAATCTAACACCATCTAATTGAATAATTGgaagaa                                                                                                   >  2:3538809/1‑128 (MQ=255)
                           taatTGATTGTGCACACATTTCTTTAACTTTTTTTAATCTTTCATTTCAAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATg                                                                                                 <  2:3866273/128‑1 (MQ=255)
                            aatTGATGGTGCACCAATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                                                                                <  1:4307194/128‑1 (MQ=255)
                             ttttatttttcACCCATTTTTTTAAATTTTTTTAATCTTTCATTTATATAAATTATTATTATATATAAATAATTATTTTCAGAAGTAGAAAATCTAACACCTTCTAATTTAATAATTGTACGAATGTc                                                                                               <  2:6529316/118‑1 (MQ=255)
                                gATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtgg                                                                                            <  1:2689409/128‑1 (MQ=255)
                                 aTGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATATCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                             >  1:4118798/1‑110 (MQ=255)
                                  tGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtg                                                                                          >  1:5559532/1‑128 (MQ=255)
                                     tGCTCCCATTTGTTTTAATTTTTTTAAGATTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                       <  2:1976833/128‑1 (MQ=255)
                                     tGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                       >  2:2413790/1‑128 (MQ=255)
                                     tGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                       >  2:6265353/1‑128 (MQ=255)
                                      gCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAAt                                                                                      >  2:906798/1‑128 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     >  2:2116220/1‑128 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     <  2:589231/128‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     <  1:1114284/128‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     >  1:2136649/1‑128 (MQ=255)
                                          ccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACt                                                                                  <  1:1921166/128‑1 (MQ=255)
                                           cATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTg                                                                                 >  1:461790/1‑128 (MQ=255)
                                            aTTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTgt                                                                                >  1:4195627/1‑127 (MQ=255)
                                              ttCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGaa                                                                              <  1:1567259/128‑1 (MQ=255)
                                                   tAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAct                                                                         <  1:3051398/128‑1 (MQ=255)
                                                   tAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAct                                                                         <  1:6321628/128‑1 (MQ=255)
                                                   tAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAct                                                                         >  2:232987/1‑128 (MQ=255)
                                                    aaCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActc                                                                        <  1:1337539/128‑1 (MQ=255)
                                                    aaCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActc                                                                        <  2:1180419/128‑1 (MQ=255)
                                                     aCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActct                                                                       >  1:3595272/1‑128 (MQ=255)
                                                     aCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActct                                                                       <  1:3318737/128‑1 (MQ=255)
                                                     aCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActct                                                                       <  1:3192681/128‑1 (MQ=255)
                                                     aCTTTTTTTAATCTTTCATTTCTAATAATGAATCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAATGAATAATTGGACGAATGTCTACTTGAATATCTGCAAATActct                                                                       >  1:1445249/1‑128 (MQ=255)
                                                      cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtgg                                                                                            >  2:1437865/1‑106 (MQ=255)
                                                      cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                      <  2:2208754/128‑1 (MQ=255)
                                                       tttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                     >  1:6565959/1‑128 (MQ=255)
                                                         tttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATa                                                                   <  2:662608/128‑1 (MQ=255)
                                                           tttATTATTTGATTTCTAATAATGATTCTTATATATAAATCATTAATATCAGAAGTAGTAAATCTAACACCATCTAATTGATTAATTGGACGAATGTCTGGTGGAATTACTGGAATTACTCTCATAAc                                                                 <  1:5679050/128‑1 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                             <  1:100328/128‑1 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATCGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                             >  1:17944/1‑128 (MQ=255)
                                                                tCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattc                                                            >  1:453136/1‑128 (MQ=255)
                                                                tCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattc                                                            <  1:4746701/128‑1 (MQ=255)
                                                                   ttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcatt                                                         <  2:4604474/128‑1 (MQ=255)
                                                                   ttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcatt                                                         <  2:1894929/128‑1 (MQ=255)
                                                                    tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGATTAACTGGAATTTCTCTCAGAACCattcattc                                                        >  2:3764441/1‑128 (MQ=255)
                                                                    tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTCGTGGAATAACTGGAATTACTCTCATAACCattcattc                                                        >  2:4736989/1‑128 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATTACTGGAATTACTCTCATAACCAttcattca                                                       >  1:518708/1‑128 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattca                                                           >  1:671657/1‑124 (MQ=255)
                                                                        ttCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGa                                                    <  2:3429884/128‑1 (MQ=255)
                                                                         tCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAGTCCTTCAGGAc                                                   >  2:4949630/1‑128 (MQ=255)
                                                                           taataaTGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAAGAACTGGAATTAATCTCATAACCAGTAATTCATCACGt                                                 >  2:4136824/1‑128 (MQ=255)
                                                                              taatGATTATTCTATATAAATCATTAATTTCAGAAGTATTAACTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTgg                                              <  2:5203456/128‑1 (MQ=255)
                                                                               aatGATTCTTCTATATATATAATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                             <  1:5811475/128‑1 (MQ=255)
                                                                               aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                             <  2:5330500/128‑1 (MQ=255)
                                                                               aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                             <  1:4865545/128‑1 (MQ=255)
                                                                               aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAAAAAAAACTAAATGAATATGTGTACGAATGTCTCGATGAATAAGAGGCAGTAGTCTCATACCCATTAATTCAGGAAGTAggg                                             >  2:2899885/1‑127 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACTAATGTCTGGTGGAATAACTGGAATTACTCACATAAACATTCTATCAGGACGTTGGtg                                            >  1:2932806/1‑127 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGGATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            >  1:5012546/1‑128 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            <  1:5314147/128‑1 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            >  2:3318109/1‑128 (MQ=255)
                                                                                 tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCCGTCATTCAGGACGTTGGTTa                                           >  2:4007371/1‑128 (MQ=255)
                                                                                 tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTa                                           >  2:4845247/1‑128 (MQ=255)
                                                                                       ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGTAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAAAGt                                     >  2:3221657/1‑128 (MQ=255)
                                                                                       ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGCACGt                                     >  2:2647090/1‑128 (MQ=255)
                                                                                          tatataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtttt                                  >  2:6016859/1‑128 (MQ=255)
                                                                                           atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACg                                                  <  1:4431541/111‑1 (MQ=255)
                                                                                           atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTa                                 <  2:3092653/128‑1 (MQ=255)
                                                                                            tctaAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                <  1:3836362/126‑1 (MQ=255)
                                                                                              taAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAg                              >  1:6164572/1‑128 (MQ=255)
                                                                                                  tCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATc                          <  2:1760339/128‑1 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATACCCATTCATTCAGGACGTTGGTTAGAACGTGTTAAAGCATCataa                      >  2:741610/1‑128 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGATCGTTTTAAAGCATCaaaa                      >  2:1067692/1‑128 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                      >  2:2997432/1‑128 (MQ=255)
                                                                                                         tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGtt                   <  2:4533862/128‑1 (MQ=255)
                                                                                                          ttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTTGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAAACATTCATTCAGGACGTTGGTTAGAACTTTTTACAGCATCAAAAGttt                  >  1:2406957/1‑128 (MQ=255)
                                                                                                           tCAGAAGTAGTATATCTAACAACATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTc                 <  2:3658424/128‑1 (MQ=255)
                                                                                                           tCAGAAGTAGTAAATCAAACACCATCTACTTGAATAATTGGCCGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAATCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTc                 <  1:2809117/128‑1 (MQ=255)
                                                                                                             tgAAGTAGTAAATGTAACAGCATCTTATTGAATAATTCGACGAATGTCTGGTTTAATAACTGGAATTAGAATCATAACCATTCATTCAGGACGTTGGTTAGAAGGTTTTAAATCATCAAAAGTTTCTa               <  1:2014994/127‑1 (MQ=255)
                                                                                                             aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTa               >  1:1223214/1‑128 (MQ=255)
                                                                                                              gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTaa              >  1:3706679/1‑128 (MQ=255)
                                                                                                              gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTGTTTCAGCATCAAAAGTTTCTaa              >  2:2498811/1‑128 (MQ=255)
                                                                                                               aaGTAGTAAATCTAACTCCATCTAATTGAATAATTGGACGAATGTATGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTaaa             <  2:560342/128‑1 (MQ=255)
                                                                                                                agtagtAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAAc            <  2:5085285/128‑1 (MQ=255)
                                                                                                                   agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAATCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCATAAGTTTCTAAACCtt         >  1:5095629/1‑128 (MQ=255)
                                                                                                                    gtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAGTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTATACGttt        >  2:4379286/1‑128 (MQ=255)
                                                                                                                     taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtttt       <  2:1322421/128‑1 (MQ=255)
                                                                                                                      aaaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGATTCTAAACGTTTta      >  2:2741140/1‑128 (MQ=255)
                                                                                                                         tCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaata   >  1:5470025/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAA  >  CP016816/478613‑478862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: