Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 516,354 A→T intergenic (+185/+321) JCVSYN2_02375 → / ← JCVSYN2_02380 antitermination protein NusG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816516,3540AT99.8% 10385.4 / ‑5.5 3431intergenic (+185/+321)JCVSYN2_02375/JCVSYN2_02380antitermination protein NusG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (3/5);  new base T (1532/1888);  total (1537/1894)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3432 total aligned reads displayed.

AATAAAAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTA  >  CP016816/516232‑516477
                                                                                                                          |                                                                                                                           
       aCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTATAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAttt                                                                                                                 <  2:597309/128‑1 (MQ=255)
       aCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAttt                                                                                                                 >  2:280427/1‑128 (MQ=255)
       aCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAttt                                                                                                                 >  1:5779514/1‑128 (MQ=255)
         aTAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTa                                                                                                               <  1:6207400/128‑1 (MQ=255)
          tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAATTTTTAATAAAAAAACATTTTAg                                                                                                              >  1:3520840/1‑128 (MQ=255)
           aaaaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGt                                                                                                             >  1:5262674/1‑128 (MQ=255)
              atatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTATGTGTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAtt                                                                                                          >  2:6353206/1‑128 (MQ=255)
                atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                        >  2:6212516/1‑128 (MQ=255)
                atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                        >  2:4062212/1‑128 (MQ=255)
                atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                        >  2:3549491/1‑128 (MQ=255)
                atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                        >  2:3682455/1‑128 (MQ=255)
                 tatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGc                                                                                                       >  2:3351391/1‑128 (MQ=255)
                 tatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGc                                                                                                       <  2:345934/128‑1 (MQ=255)
                  atTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCa                                                                                                      >  2:4476084/1‑128 (MQ=255)
                   tttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCaa                                                                                                     >  1:5580508/1‑128 (MQ=255)
                     tttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAtt                                                                                                   >  1:1772045/1‑128 (MQ=255)
                      ttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATta                                                                                                  <  1:694538/128‑1 (MQ=255)
                       tAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtat                                                                                                 >  1:4631123/1‑128 (MQ=255)
                        aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCa                                                                                                      >  2:4893004/1‑122 (MQ=255)
                        aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtata                                                                                                >  2:2106334/1‑128 (MQ=255)
                            aaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGTGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaaa                                                                                            <  1:1415490/128‑1 (MQ=255)
                            aaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaaa                                                                                            <  2:1466477/128‑1 (MQ=255)
                            aaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaaa                                                                                            <  1:2117956/128‑1 (MQ=255)
                              tatGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAg                                                                                          >  1:5056919/1‑128 (MQ=255)
                               atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                         <  1:4074480/128‑1 (MQ=255)
                                tatttttATAAGATTAGAAAAAAAGGTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTATAATTTTAATTAAAAAACATTTTAGTATTTGCAATTATAAAAAAGtt                                                                                        <  1:1295087/126‑1 (MQ=255)
                                tGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGtt                                                                                        <  1:3364736/128‑1 (MQ=255)
                                 gTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTATTATTTTTAATTAAAATTTTAATAAAAAAACATATTAGTATTTGCAATTATAAAAAAGttt                                                                                       >  1:37340/1‑128 (MQ=255)
                                   ttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTAt                                                                                     <  1:3961925/128‑1 (MQ=255)
                                    tttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATg                                                                                    >  2:5097407/1‑128 (MQ=255)
                                      tataAGATTAGAAAAAAAGCTTATTTTAAGTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGat                                                                                  <  2:2908454/128‑1 (MQ=255)
                                        taATATTATTAAAAAATCTTATTTTAATGGCTTTTTTTAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAATAAAAAATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                <  1:3635550/128‑1 (MQ=255)
                                         taagttactaaaaaaTCCTATTTTTATGGGATTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataat                                                                               <  2:2576273/119‑1 (MQ=255)
                                         aataTTATAAAAAAATCTTATTTTAAAGGCTTTTTTAAATATTATTTAAAATTTTTTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataat                                                                               <  1:3222564/125‑1 (MQ=255)
                                          aGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                              <  2:5591010/128‑1 (MQ=255)
                                          aGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                              <  2:5219457/128‑1 (MQ=255)
                                           gATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAatat                                                                             <  2:1995918/128‑1 (MQ=255)
                                            aTTAGAAAAAAAGATTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAAtata                                                                            <  2:1895161/128‑1 (MQ=255)
                                             ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                           <  2:3965331/128‑1 (MQ=255)
                                              tAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAcc                                                                          >  1:1998482/1‑128 (MQ=255)
                                               aGAAATAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCt                                                                         <  2:5475757/128‑1 (MQ=255)
                                                gAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                        <  1:236493/128‑1 (MQ=255)
                                                gAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                        >  1:1876350/1‑128 (MQ=255)
                                                   aaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTa                                                                     >  2:1089958/1‑128 (MQ=255)
                                                   aaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACATATTa                                                                     >  1:3085602/1‑128 (MQ=255)
                                                    aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                           <  2:1340013/121‑1 (MQ=255)
                                                    aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                    >  2:1778606/1‑128 (MQ=255)
                                                      aaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAata                                                                  >  2:3214907/1‑128 (MQ=255)
                                                       aGCTTATTTTAAGGGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAatat                                                                 <  2:4990380/128‑1 (MQ=255)
                                                         cTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAg                                                               >  2:3001609/1‑128 (MQ=255)
                                                            ttaaaataagCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                            >  1:4852562/10‑128 (MQ=255)
                                                            aTTTTAAGGTCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                            <  1:6491010/128‑1 (MQ=255)
                                                            aTTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                            <  1:1644722/128‑1 (MQ=255)
                                                             ttttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                         >  2:1584392/1‑98 (MQ=255)
                                                             ttttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATa                                                           >  1:2580921/1‑128 (MQ=255)
                                                              tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                          <  2:4353903/128‑1 (MQ=255)
                                                              tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                          <  1:1004051/128‑1 (MQ=255)
                                                               ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                         >  1:5914241/1‑128 (MQ=255)
                                                               ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                         <  2:2262601/128‑1 (MQ=255)
                                                                  aGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaa                                                      <  2:3776849/128‑1 (MQ=255)
                                                                  aGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaa                                                      >  1:344323/1‑128 (MQ=255)
                                                                  aGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaa                                                      <  2:4193304/128‑1 (MQ=255)
                                                                       ttttttAAAGATTATTTAATATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGa                                                 <  1:6373638/128‑1 (MQ=255)
                                                                           ttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                            <  2:5348909/113‑1 (MQ=255)
                                                                           ttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAag                                             <  1:6581307/128‑1 (MQ=255)
                                                                           ttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAag                                             >  1:4349133/1‑128 (MQ=255)
                                                                            tAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAaga                                            <  1:11621/128‑1 (MQ=255)
                                                                            tAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAaga                                            <  1:3843460/128‑1 (MQ=255)
                                                                             aaaGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAagag                                           >  2:735429/1‑128 (MQ=255)
                                                                                  ttattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAata                                      <  2:1709354/128‑1 (MQ=255)
                                                                                  ttattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAata                                      <  1:814451/128‑1 (MQ=255)
                                                                                   tattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGaaa                                        <  1:2937003/125‑1 (MQ=255)
                                                                                   tattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAatat                                     >  1:2806662/1‑128 (MQ=255)
                                                                                    attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                    <  2:5865022/128‑1 (MQ=255)
                                                                                    attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                    <  2:4634655/128‑1 (MQ=255)
                                                                                     tttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatta                                   >  1:5419907/1‑128 (MQ=255)
                                                                                      ttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtattat                                  >  1:6039488/1‑128 (MQ=255)
                                                                                      ttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtattat                                  <  1:1541741/128‑1 (MQ=255)
                                                                                       tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                 <  2:856001/128‑1 (MQ=255)
                                                                                         aaaTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAg                               >  1:2228517/1‑128 (MQ=255)
                                                                                             tttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATg                           <  2:5693509/128‑1 (MQ=255)
                                                                                              ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                          >  2:475798/1‑128 (MQ=255)
                                                                                              ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                          >  1:2497982/1‑128 (MQ=255)
                                                                                              ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                          >  2:2480848/1‑128 (MQ=255)
                                                                                              ttGTTTTTATTTTTAATTAAAATTTTAACAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                          <  2:2367781/128‑1 (MQ=255)
                                                                                               tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATTAACAACTAGAAAAGAGAAATATTATTAGTATGAt                         >  2:2682992/1‑128 (MQ=255)
                                                                                                  ttttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGc                      <  2:1228235/128‑1 (MQ=255)
                                                                                                      atttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCTaag                  >  2:6092693/1‑127 (MQ=255)
                                                                                                      atttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaat                  >  1:420005/1‑128 (MQ=255)
                                                                                                       tttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaatt                 >  2:3120366/1‑128 (MQ=255)
                                                                                                        ttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaatta                <  1:4953065/128‑1 (MQ=255)
                                                                                                         tttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATTATAGCGaattaa               >  1:949589/1‑128 (MQ=255)
                                                                                                             attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt           <  1:4449751/128‑1 (MQ=255)
                                                                                                              ttaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttttt          <  2:3849280/128‑1 (MQ=255)
                                                                                                              ttaaAATTATAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATTGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttttt          <  1:5145341/128‑1 (MQ=255)
                                                                                                               taaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTa         <  2:6455905/128‑1 (MQ=255)
                                                                                                                aaaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAATCAACTAGAAAAGAGAAATATTATTAGTATGATAGAGAATTAATTTTTaa        >  1:2676675/1‑128 (MQ=255)
                                                                                                                  aaTTTTAATAAAATAACATTTTAGTATATTCAATTATAAAAAAGTTTATGATAATTTACCTATAAATATAGAATAAACTACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa      <  1:4723965/128‑1 (MQ=255)
                                                                                                                  aaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa      >  1:5665656/1‑128 (MQ=255)
                                                                                                                   aTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTATAAAAGAGAAATATTATTAGTATGATAGCGAATTAATATTTaaaag     >  2:6597617/1‑127 (MQ=255)
                                                                                                                          |                                                                                                                           
AATAAAAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTA  >  CP016816/516232‑516477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: