Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.7% 7102.1 / 13.4 2442intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (17/40);  new base T (1533/852);  total (1550/892)
Fisher's exact test for biased strand distribution p-value = 2.11e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2533 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346
                                                                                                                              |                                                                                                                                 
tatagtttATTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                 <  1:6069667/123‑1 (MQ=255)
  tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaa                                                                                                                               >  1:4421439/1‑127 (MQ=255)
   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                              >  1:5415105/1‑126 (MQ=255)
    cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                             >  2:2422702/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  2:5259810/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  1:3624042/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  1:674431/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAATTTTTAAAAaat                                                                                                                            >  1:5988902/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGAAAATTTTTAAAAaattaat                                                                                                                        >  1:5888763/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                        >  2:183923/1‑128 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      >  1:2445824/1‑128 (MQ=255)
            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                         <  1:2929715/124‑1 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                    <  2:5413842/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCTCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAATTTTTAAATAATTAATTTGaa                                                                                                                   >  2:2320869/1‑128 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   >  2:4690673/1‑128 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   <  1:3791940/128‑1 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTTTATGAATTAGTAAGAAACTAAACCTATTATCTTTTTAGATATATTTTGTATATCCAAATTTTTAAAAAATTAATATGAACTAGt                                                                                                              >  1:6522880/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  1:743310/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  2:1986573/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  2:3260993/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  2:6253695/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             <  2:1102969/128‑1 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             >  2:3685809/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             >  1:567560/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTAACTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             >  1:5823240/1‑128 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                            <  2:1680736/128‑1 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                            <  1:1103363/128‑1 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTaaa                                                                                                           >  2:4393761/1‑128 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   <  2:6131528/120‑1 (MQ=255)
                       ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                          <  1:5220297/128‑1 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                        >  1:3064160/1‑128 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                     >  2:6393638/1‑128 (MQ=255)
                            tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                    >  2:2211110/1‑128 (MQ=255)
                            tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                    >  1:5330324/1‑128 (MQ=255)
                              ataataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCt                                                                                                  >  1:3338789/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttt                                                                                                >  1:4065739/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                >  1:4907808/1‑128 (MQ=255)
                                aataTTTAGTCTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                >  1:5475909/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTAGTAAAAAATATCttat                                                                                               >  2:680343/1‑126 (MQ=255)
                                     ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttt                                                                                           >  1:1910888/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATAttttttttt                                                                                          >  1:2048130/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                          >  1:6245844/1‑128 (MQ=255)
                                       aGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttt                                                                                         >  2:5526521/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                        >  2:3439765/1‑128 (MQ=255)
                                          ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttttttttttttt                                                                                      >  1:1000505/1‑128 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                     >  2:5913491/1‑128 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                    >  2:4518507/1‑128 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAATAGCttttttttttttttt                                                                                    >  2:6547163/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                   >  1:1473451/1‑128 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttttta                                                                              >  2:2671025/1‑126 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttatttt                                                                              >  1:1553052/1‑126 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataat                                                                              >  2:8061/1‑123 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttattttttaaaa                                                                              >  1:6160285/1‑124 (MQ=255)
                                                    tttagCACTTTTATTAAATAGTAAGAAAATAAAAATATTATTTTTTTATTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAAAAGCTTTTTTTTTTTTTTTTtataatg                                                                            <  1:4761930/124‑4 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttatatttaa                                                                         >  2:577342/1‑120 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAAtta                                                                         >  1:2805869/1‑126 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTAGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAAttt                                                                         >  2:3009631/1‑126 (MQ=255)
                                                        ttaattGTTTTAATTATTAATAAAATAAAAATATTATATTTTTATTTATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGaa                                                                       <  2:5152882/124‑1 (MQ=255)
                                                         cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCGAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAtttaaa                                                                      >  1:1789245/1‑123 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtttatattttaaa                                                                    >  2:3590553/1‑119 (MQ=255)
                                                              gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAattaaa                                                                 >  1:4532356/1‑124 (MQ=255)
                                                              gTATGAAATAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAATTAGCTTTTTTTTTTTTTTTTTTTATTTGTAAATATaa                                                                 >  2:3806995/1‑128 (MQ=255)
                                                               tattaattattaatAAAATAAAAATATTTTTTTTTTATATATTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                 <  2:1424785/128‑1 (MQ=255)
                                                                aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTTGAAATATAAAttt                                                             >  2:5609159/1‑128 (MQ=255)
                                                                   aaTTAGTAATAAAATAAAAATATTATATTTTTATATATTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTGTAATTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                           <  1:938331/128‑1 (MQ=255)
                                                                   aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGATAATATAATttttt                                                           >  2:4293356/1‑127 (MQ=255)
                                                                     ttttttttaaattaaaaattTTATTTTTTTATATATTTTTTGTATATTAAATTTTTTTAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                          <  2:4907131/120‑1 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATTATGTAAATAAAATTTTTat                                                          >  2:2920744/1‑127 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATTTAAAAAAAAATTTTaaa                                                         >  1:4664978/1‑126 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTATAATATAAATTTTAgtt                                                        >  1:6381285/1‑126 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTattaatttttt                                                 >  1:4273099/1‑118 (MQ=255)
                                                                             aaaattaaaataTTATATTTTTATTTATTTTTTTTATTTTTAAATTTTTAAAAAATTTATTTTTAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTttg                                                  <  2:3096445/122‑1 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTTTTAATTTAAATAAAAATTTTatttttttttt                                                 >  1:5555197/1‑118 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTAAAAATTGAAATAAAAATATTAGTTAATttattt                                               >  1:847736/1‑127 (MQ=255)
                                                                                      attttttttttttATTTATTTTTTTTTTTTTTAAATTTTTAAAAAATTTAATTTTAATTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  1:5028417/120‑1 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATttttttttatatt                                         >  1:235192/1‑121 (MQ=255)
                                                                                          tataTTTTTAAATTATTTTTTTAAATTAAAATTTTTAAAAAATAAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                     <  2:277889/128‑1 (MQ=255)
                                                                                            tatttttttattttttttttatttTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAATAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                     <  1:3480832/126‑1 (MQ=255)
                                                                                            tCTTTTTATATATTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTa                                   <  1:5139330/128‑1 (MQ=255)
                                                                                                    tattttttttGTATTATTAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  1:1375707/124‑1 (MQ=255)
                                                                                                        ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTTTTTTTTTTTATATTTATATAAAAAAATATaa                        >  2:533228/1‑128 (MQ=255)
                                                                                                        ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATATaa                        >  1:2678938/1‑128 (MQ=255)
                                                                                                         ttttttttTATTTAAATTTTTTAAAAATTAATTTTTAATTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                    <  2:1293264/128‑1 (MQ=255)
                                                                                                           tttGTTTATTCAAATTTTTTAAAAAATAATTTTTACTTGTTAAAAATA‑‑TTATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                   <  1:4185109/128‑1 (MQ=255)
                                                                                                           tttGTTTATTAAAATTTTTATAAAATTAATTTTAACTTTTTAAAAAATTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                    <  2:5028032/128‑1 (MQ=255)
                                                                                                                tattttaaatttttTAAAAAATAAATTTAAATTTTTAAAAAAA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  1:2250473/118‑1 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAACTTATTTTTTGGAATAATTAAATAAAAAAAAAGAAATAAAAAAatat            >  2:4173522/1‑128 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAATAttt          >  1:3202026/1‑127 (MQ=255)
                                                                                                                    ttaaaTTTTTAAAAATTTTTTTTTAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGt         <  2:2115886/126‑1 (MQ=255)
                                                                                                                     taaaTTTTTAAAAAATAAATTTTAACTTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTg          <  2:6008242/127‑1 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTCTAATAATATAAAAAAATAAAAATAaaaaaaatttt          >  2:255545/1‑123 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTTTCTTATTATAAAAAAAAATAAAAAGTAAAAAAAAtttt         >  1:1229807/1‑126 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTAAAAATTTAAAAAAAAATTTTATTTATTTTTTTTTATAATTTTAAAAAAAAATAAAATCAAAAAataattta        >  2:1484083/1‑123 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATTTTTTTTTTTTTATAATTATATAAAAAAATAAAAAGTAAAAAattatt          >  1:1669770/1‑124 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATGTATTTTTTTGTAAATTTATCTAAAAAAATAAAAATGAAAAAATtttttt        >  1:574038/1‑128 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGGAAATATAAATTTTATGTATTTTGTTTTATAATTATATAAAAAAAAAGAAAGGAAAAAATATTTTTa       >  1:3908566/1‑128 (MQ=255)
                                                                                                                       aaTTTTTTAAAAAATAATTTTTAATTTTTAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa       <  2:1588108/128‑1 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTTTTTTTTTGTGTAATTATAAAAAAAAATATAAAGGAAAAAATATtttt        >  1:2165649/1‑128 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTAAAAATAATTTTTAGTTTTTTTTTTTTAAAATAATATAAAAAATTATAAAGAAAAAAATTTTTTTa       >  2:4031596/1‑128 (MQ=255)
                                                                                                                         tttttTAAAAAATTTATTTTATATTTTTAAAAATTGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa       <  1:2562921/127‑1 (MQ=255)
                                                                                                                              |                                                                                                                                 
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: