BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,163	2 bp→TT	intergenic (‑446/+396)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,163	0	A	T	99.6%	7805.6 / NA	2496	intergenic (‑446/+397)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (8/3); new base T (1323/1158); total (1333/1163)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,164	0	A	T	98.7%	7008.4 / NA	2465	intergenic (‑447/+396)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (3/29); new base T (1314/1117); total (1318/1147)
Fisher's exact test for biased strand distribution p-value = 2.13e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2506 total aligned reads displayed.

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATG  >  CP016816/420038‑420289

||

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATa                                                                                                                               <  2:2661380/128‑1 (MQ=255)

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATa                                                                                                                               <  1:3685781/128‑1 (MQ=255)

   cattaatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATa                                                                                                                               <  2:3355537/128‑1 (MQ=255)

     ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAAc                                                                                                                             <  1:5431881/128‑1 (MQ=255)

      tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACt                                                                                                                            >  1:4719762/1‑128 (MQ=255)

        atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGa                                                                                                                          <  1:1134303/128‑1 (MQ=255)

          aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTATAAAAATACTAGCACTTTTTA‑AAAAAATTTTTAATTTTAATAACTGAat                                                                                                                        >  2:6209064/1‑128 (MQ=255)

           cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                       <  1:3813921/128‑1 (MQ=255)

            ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                      >  1:6643813/1‑128 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                     <  1:2190851/128‑1 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                     >  2:4463652/1‑128 (MQ=255)

              ttAGTCTCATAAATCTAAGTTTCTAGAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                    <  1:6272957/128‑1 (MQ=255)

               tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTT‑TAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                                   >  2:1621064/1‑128 (MQ=255)

                  tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                                >  1:3445399/1‑128 (MQ=255)

                    tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                              <  2:3136883/128‑1 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                            >  1:2739860/1‑128 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAAAAACTGAATTATTTTTCAAAt                                                                                                            >  2:2016471/1‑128 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTA‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                            >  2:1897574/1‑128 (MQ=255)

                       tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                           <  2:3872508/128‑1 (MQ=255)

                       tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                           <  1:2620057/128‑1 (MQ=255)

                        aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAttt                                                                                                          >  1:4445099/1‑127 (MQ=255)

                         aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                         <  2:4175534/128‑1 (MQ=255)

                         aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                         <  1:3965553/128‑1 (MQ=255)

                           tatAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                       <  2:119330/126‑1 (MQ=255)

                              tacgatatAAAATTATTTTATCATTTATAAAAATAATAATTTTATTATTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                    <  2:2271657/121‑1 (MQ=255)

                              aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                    >  1:283549/1‑128 (MQ=255)

                               aGTTTATAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                   <  2:6352573/128‑1 (MQ=255)

                                 tttattaaaTTATTTTATTATTTTAAAAAATTATAATTTTTTTAGTTTTTTTTTTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                                 <  2:522349/122‑1 (MQ=255)

                                 tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                                 <  2:5003150/128‑1 (MQ=255)

                                 tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                                 <  2:5528269/128‑1 (MQ=255)

                                  ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTATT‑AAAAAATTATTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                                >  1:3082418/1‑128 (MQ=255)

                                   tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                               <  1:3550126/128‑1 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              >  1:1974574/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              >  2:1710830/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              >  1:5590268/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              >  2:211402/1‑128 (MQ=255)

                                     tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTATTAT‑AAAAAATTTTTAATTTAAATATCTGAATTATTTTACAAATTGTTAAATTATTTTc                                                                                             >  1:4222480/1‑128 (MQ=255)

                                      aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                       <  1:5698563/117‑1 (MQ=255)

                                      aaaaTTATTTTATCATATAAATAAATAATACTATTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                            <  2:299038/128‑1 (MQ=255)

                                       aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                    >  2:6478840/1‑119 (MQ=255)

                                       aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                           <  1:5234504/128‑1 (MQ=255)

                                       aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                    <  1:6478840/119‑1 (MQ=255)

                                        aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                          <  1:2489950/128‑1 (MQ=255)

                                        aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                          >  1:5516156/1‑128 (MQ=255)

                                           tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                             >  2:3462749/1‑122 (MQ=255)

                                           tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTCTTAAATTATTTTCAAATCa                                                                                       >  1:1298372/1‑128 (MQ=255)

                                             ttttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                      <  2:849375/128‑1 (MQ=255)

                                             ttttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAtt                                                                                     >  2:4303399/1‑128 (MQ=255)

                                              tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                    >  2:5450861/1‑128 (MQ=255)

                                               ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                   >  1:619935/1‑128 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                  <  1:656169/128‑1 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                  <  2:6070663/128‑1 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                  <  2:5576382/128‑1 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                  <  1:2287406/128‑1 (MQ=255)

                                                 aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                                 <  1:2945743/128‑1 (MQ=255)

                                                  tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                                >  2:1373162/1‑128 (MQ=255)

                                                  tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                                >  1:3339756/1‑128 (MQ=255)

                                                      ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                            >  1:5926750/1‑128 (MQ=255)

                                                             aataataatTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                     <  2:2524988/128‑1 (MQ=255)

                                                              ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                    <  2:4723817/128‑1 (MQ=255)

                                                              ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAACAAtttt                                                                    >  2:5103236/1‑128 (MQ=255)

                                                                  taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                                >  1:1569607/1‑128 (MQ=255)

                                                                    cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑Tc                                                            >  1:4560075/1‑128 (MQ=255)

                                                                     ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATtc‑‑tct                                                           >  1:1002581/1‑127 (MQ=255)

                                                                      tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                         <  2:4590589/99‑1 (MQ=255)

                                                                        tattaGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTC‑‑TC‑G‑tat                                                      >  1:4311673/1‑126 (MQ=255)

                                                                        tattaGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGA‑‑TC‑G‑ttt                                                      >  1:1892124/1‑128 (MQ=255)

                                                                         attaGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑tttt                                                     >  1:5352853/1‑128 (MQ=255)

                                                                                   atatTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑tttttttttttttt                                           >  2:6575238/1‑128 (MQ=255)

                                                                                    tatTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑ttttttttttttttt                                          >  1:6365233/1‑128 (MQ=255)

                                                                                     atTAGATAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATATTATTC‑‑TC‑G‑tattttttttttttat                                         >  1:6030075/1‑126 (MQ=255)

                                                                                       tAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑tttttttttttttttttt                                       >  1:2391820/1‑127 (MQ=255)

                                                                                       tAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTa                                       >  2:3750383/1‑128 (MQ=255)

                                                                                       tAGAAAACTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATCTATTAATAAATTTTATGC‑‑TA‑G‑TTTTTTTTTTTTTTTTTa                                       >  1:5058119/1‑128 (MQ=255)

                                                                                          taatattttttcACTTTTTT‑AAAAATATTTTTTTTTTAATAAATTAATTTTTTTTCAATTTGTTAAATTATTTTCATATCATTATCTATTAATAAATTTTTTGT‑‑TC‑G‑TTTTTTTTTTTTTTTTTACaa                                    <  2:2803225/117‑1 (MQ=255)

                                                                                           aaaCTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTAaaaa                                   >  2:2412013/1‑128 (MQ=255)

                                                                                            aaCTACTAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTACaaaa                                  >  2:5932484/1‑128 (MQ=255)

                                                                                             actactAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTTaaaaaa                                 >  2:5727898/1‑128 (MQ=255)

                                                                                             actactAGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTAaaaaaa                                 >  1:3211289/1‑128 (MQ=255)

                                                                                              attatataaatttttt‑AAAAAATTTTTAATTTTAATAAATTTATTTTTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTTTTC‑‑TC‑GTTTTTTTTTTTTTTTTTTACaaaaa                                 <  1:1407699/118‑1 (MQ=255)

                                                                                              atactaTCACTTTTTT‑AAAAAATTTTTAAATTTAATAAATTAATTATTTTTCAAATTTTTAAATTTTTTTAAAATAATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTACAAAAat                                <  2:405916/127‑1 (MQ=255)

                                                                                               tactaGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTAAAAAAata                               >  1:1731837/1‑128 (MQ=255)

                                                                                                actaGCACTTTTTT‑AAAAAATTTTTAATTTTAATAAATGAATTATTTTTAAAATTTTTAAATTATTTACAAATCATTATATTTTAATAATTTTTATGA‑‑TC‑G‑TTTTTTTTTTTTTTTTTAAAAAAatat                              >  2:750761/1‑128 (MQ=255)

                                                                                                aatataaatttttt‑AAAAAATTTTTAATTTTAATAATTTAATTTTTTTTTAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTATGA‑‑TC‑G‑TTTTTTTTTTTTTTTTTACAAAAatat                              <  2:3585477/120‑1 (MQ=255)

                                                                                                  taGCACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAat                           >  2:5659966/1‑128 (MQ=255)

                                                                                                     cACTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAatt                          >  1:512221/1‑128 (MQ=255)

                                                                                                      aCTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                     <  1:802101/87‑1 (MQ=255)

                                                                                                      aCTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑GTTTTTTTTTTTTTTTTTTAAAAAAAAATaaata                         >  1:6105170/1‑125 (MQ=255)

                                                                                                       cTTTTTT‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TCTT‑TTTTTTTTTTTTTTTTTAAAAAAATAT‑atttat                       >  2:5834810/1‑128 (MQ=255)

                                                                                                         ttttt‑AAAAAATTTTTTATTTTAATTAATTAATTTTTTTTTAAATTGTTATATTATTTTAAATTATTTATTTTTTTATTAATTTTATTT‑‑TT‑T‑TTTTTTTTTTTTTTTTTACAAAAATATAatttattt                     <  2:100656/128‑1 (MQ=255)

                                                                                                           ttt‑tAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTTAATTTTTTTAAAATAATTATATATTAATAAATTTTTTTCTATT‑T‑TTTTTTTTTTTTTTTTTACAAAAATATAatttattt                     <  1:4854135/128‑1 (MQ=255)

                                                                                                           ttt‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTTAAATTTTTAAATTATTTTTAAATAATTATATATTAATAAATTTTATGC‑‑TC‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAt                  <  1:580868/128‑1 (MQ=255)

                                                                                                           ttt‑AAAAAATTTTTAATTTTAATAAATTAAATATTTTTTAAATTTTTAAATTATTTTTAAAAAATTTTATATTAATAAATTTTATGC‑‑TC‑GTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTg                    <  2:683278/128‑1 (MQ=255)

                                                                                                             tataaaaaTTTTATTTTTTAATAATTTATTTTTTTTTAAATTTTTTAATTTTTTTTTAATTATTTTTCTTTTATATATTTTTTTGG‑‑TG‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt                 <  1:1633001/125‑1 (MQ=255)

                                                                                                             t‑AAAAAATTTTTAATTTTAATAATTTTATTATTTTTTAAATTTTTAAATTTTTTTAAAATAATTATATATTAATAAAATTTATTT‑‑TC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt                 <  1:2080450/128‑1 (MQ=255)

                                                                                                             t‑AAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATAATTATTTTTAtt                 >  2:1516405/1‑128 (MQ=255)

                                                                                                               aaaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATAAAATTTATTTATTTaa               >  1:4985073/1‑128 (MQ=255)

                                                                                                                aaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTaa               >  2:4873762/1‑128 (MQ=255)

                                                                                                                 aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTaaa              >  2:5339773/1‑128 (MQ=255)

                                                                                                                   aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTAATaaaag            >  1:4096635/1‑126 (MQ=255)

||

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑‑TC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATG  >  CP016816/420038‑420289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`