Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4870T.36.1% 706.0 / inf 851intergenic (+265/‑410)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (180/279);  new base . (81/178);  total (336/515)
Fisher's exact test for biased strand distribution p-value = 3.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-06

Only 100 of 908 total aligned reads displayed.

AATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTA  >  CP016816/202369‑202614
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aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAACAAACACCATaaaaaaaaaa                                                                                                                           >  1:6309285/1‑126 (MQ=255)
 ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGCTCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑Ttaaaatataaaa                                                                                                                       >  2:8609081/1‑128 (MQ=255)
 ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAATTAAAAAAAAAACAATACAC‑‑Ttaaaaattaaaa                                                                                                                       >  2:1200055/1‑128 (MQ=255)
    ataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAACAATCACCTT‑‑‑AAAAATaaaaaaa                                                                                                                    >  1:9238336/1‑128 (MQ=255)
     taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaa                                                                                                                   >  2:2072733/1‑128 (MQ=255)
         gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATATTAACAAATAAAAAAAAAAAAA‑‑‑ACAAATAAAAAATtaaaaaaaaaaaa                                                                                                              >  2:3405595/1‑128 (MQ=255)
         gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATCTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaa                                                                                                               >  2:2338458/1‑128 (MQ=255)
         gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCATCGTGATCTATTTTTTTATTTGTTTATCTAAAAAAATAAAAAAAAAAAAAACCCAAT‑‑‑AAAAATaaaaaaaaaaaaa                                                                                                              >  2:7731394/1‑128 (MQ=255)
          ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaaaaaaaaa                                                                                                             >  2:8677554/1‑128 (MQ=255)
          ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCAAC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                              >  1:7875427/1‑128 (MQ=255)
          ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTATTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAACAATAAC‑‑‑ATAAAAAATtaaaaaaaaaaaaa                                                                                                             >  1:7473720/1‑128 (MQ=255)
            aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATTTATATCCTCGTGATCTAATTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAAACAAATT‑‑AAAAATaaaaaaaaaaaaaaa                                                                                                            >  1:9110744/1‑128 (MQ=255)
            aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaaaaaaaaaaa                                                                                                           >  1:4673924/1‑128 (MQ=255)
            aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                            >  2:8002054/1‑128 (MQ=255)
            aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAAACCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                            >  1:7392052/1‑128 (MQ=255)
            aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCTTGATCTATTTATTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaa                                                                                                           >  1:5531798/1‑128 (MQ=255)
            aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCGTGATCTATTATATTATTAATTTATCTTCACAAATAAAAAAAAAACAAACC‑‑‑AAAAAAAAATaaaaaaaaaaaaaaaa                                                                                                           >  2:455234/1‑128 (MQ=255)
             aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                           >  2:7434870/1‑128 (MQ=255)
              ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAAACCC‑‑ATAAAAAATaaaaaaaaaaaaaaaaa                                                                                                          >  2:8339244/1‑128 (MQ=255)
              ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCCCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCCCC‑‑TAAAAAAATaaaaaaaaaaaaaaaaa                                                                                                          >  2:3585271/1‑128 (MQ=255)
               tttaattaaAAAAAAATAAATATAAAAATTTATTTAATTTATATACTTTTTTTTTTTTTTTTTTTTTTTTTATTTTAAAAAATAAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                         <  2:9463212/127‑1 (MQ=255)
               gTTTATTAAAAAAACATAAAATTTATAATTATTAAAATTTATATCCTACTTATAAATTTTTTTATTTTTTAAAATTAAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                         <  1:6095501/128‑1 (MQ=255)
                ttaattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCATGATATATTTTTTTAATTATTTATATTCAAAAATAAAAAAAAAAAAATAAAAAT‑‑‑AAAAATaaaaaaaaaaaaaaaaaaaa                                                                                                       >  2:1607513/1‑128 (MQ=255)
                 taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCAAAAATAAAAAAAAAACAATAAC‑‑‑ATAAAAAATtaaaaaaaaaaaaaaaaaaaa                                                                                                      >  1:4891726/1‑128 (MQ=255)
                  aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                                        >  2:1059130/1‑128 (MQ=255)
                    ttaaAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCTATTTTTTTATTTGTTTATCTAAAAAAAAAAAAAAAAAAAAAAACC‑‑‑ATAAAAAATaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                   >  2:5112586/1‑125 (MQ=255)
                    ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATATTCACAAATAAAAAAAAAACAATCACC‑‑AAAAAAAATaaaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:4313260/1‑126 (MQ=255)
                     taaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTATTTATCTTCACAAATAAAAAAAAAAACAATCAAC‑‑TAAAAAATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:2467759/1‑126 (MQ=255)
                      aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCACC‑‑TAAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  1:2475419/1‑124 (MQ=255)
                      aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATACC‑‑ATAAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  2:3138200/1‑124 (MQ=255)
                       aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  1:8704062/1‑123 (MQ=255)
                        aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  2:7176682/1‑128 (MQ=255)
                        aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  2:3279927/1‑128 (MQ=255)
                          taaaataaaaataaaaAATATTAAAATTTATATACTAGTTATCTAATTTTTTATTTTTTTATATTAAAAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAaaacaaa                                                                                           <  1:1476294/124‑1 (MQ=255)
                           aacATAAACTTAATAATTATTAAAATTTAGATACTCGTTATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaactaaaaacaaactct                                                                                             <  1:8930848/126‑10 (MQ=255)
                           aCCATAAACATAAAAATAGTTAAAATGTAGATACTCGTTTTCTATTTTTTTATTTTTTTATTTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAaaacaaac                                                                                          <  1:4508060/128‑1 (MQ=255)
                             caaaaaaTTAAAAAATCTTATAATTTATATACTTGTTTTTTTTTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAaaacaaa                                                                                           <  1:8621745/125‑1 (MQ=255)
                             cATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTAATTTTTTATTTGTTTATCTTCACAAATTAAAAAAAAACAATAACC‑‑TTAAAATAAAAAAAAAAAAAAAAAAAAAAATAAacacaaaa                                                                                          >  2:5024110/1‑127 (MQ=255)
                             aaTAAAATTAAAAATTTTTATAATTTATAAACTACAGATATAATTTTTTATTTTTTTAACATAAAAAAAAAAAAAAAAAAAAACCCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                            <  1:4288531/127‑1 (MQ=255)
                              aTAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAGAAAAACTAAAAACAAAct                                                                                         <  1:7274326/128‑1 (MQ=255)
                                 aaattataaatTATTATAATGTATATCATCCTGATATATTTTTTTATTTGTTTATATAAACAAAAAAAAAAAAAACAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                      <  1:8189443/120‑1 (MQ=255)
                                  aaataataaATATTAAAAATTAAATACTCGTTAAATATTTTTTTATTTTTTTAAATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                      <  1:8533935/125‑1 (MQ=255)
                                     taataaTTTTTATAATGTAGATCCTCTTTTTATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCtt                                                                                <  2:9243721/128‑1 (MQ=255)
                                        aaatTATTATAATTTAAAACCACGTGTTTTATTATTTTATTTGTTTATCTTAACAAATAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaa                                                                              <  2:4919312/127‑1 (MQ=255)
                                            ttttttaATGTATATCCTTCTTATATATTTTTTTATTTTTTTTTAATCAAAAATAAAAAAAAAAAAAACCACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                                           <  1:9500591/123‑1 (MQ=255)
                                            tCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAGCCC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAATAAAAAAAAACAGTATTTATTaaatt                                                                           >  2:7945677/1‑126 (MQ=255)
                                              ttataaattttaTCCTTGTTATTTATTTTTTTATTTTTTTATATAAACAAAAAAAAAAAAAACAAACCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                                           <  1:7782878/117‑1 (MQ=255)
                                                ataATTTATATCATAGTTATATTTTTTTTTATTTTTTAATATAAAAAAAAAAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTc                                                                         <  1:7622227/128‑1 (MQ=255)
                                                   ttttatattcttgTGATTTTTTTTTTTATTTTTTTATATTAAAAAATAAAAAAAAAACAATCCC‑‑‑‑‑AAAAAAAATTAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatta                                                                 <  1:6982051/116‑1 (MQ=255)
                                                   aattataatctagTGATATATTTTTTTATTTTTTTATAATAAAAAAAAAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCt                                                                      <  1:2431763/116‑1 (MQ=255)
                                                     gtttttcatatcgATTTATTTTTTTTTTTTTTTTTATTATACAATAAAAAAAAAAAAATTCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAATCTCTATTTCTTAAAATTCCCtatt                                                                  <  2:6349180/116‑1 (MQ=255)
                                                      tAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACAATATGTATTAACATTcccatttaa                                                                 >  2:957242/1‑122 (MQ=255)
                                                       tattCCTCGTTATTAATTTTTTTATTTTTTTAAATTAAAAAAAAAAAAAAAAACAAACCCCTT‑‑‑AAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                              <  2:562859/125‑1 (MQ=255)
                                                        tatactctTGATCTTTTTTTTTATTTTTTTATATTCAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                            <  1:1157722/124‑1 (MQ=255)
                                                         tttcttgttttttATTTTTTTTTTTTTTTATTTTTAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                             <  2:9363937/116‑1 (MQ=255)
                                                         tactttctgttttATTTTTTTTTTTTTTTATTTTAAAAAATAAAAAAAAAAAAATAACATTT‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                             <  2:4755903/116‑1 (MQ=255)
                                                         attatttttttttatttttttattttttTTTATTAACAAATAAAAAAAAAAAAATACCATT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                           <  2:404598/116‑1 (MQ=255)
                                                          tacTCGTTATCTATTTTTTTATTTTTTTATCATTAAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                            <  1:3222672/126‑1 (MQ=255)
                                                            tatttttatatttttttttttttttTATAATTAAAAAATAAAAAAAAAAAAATCACATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTATATTTCTTAAAATTCCCTATTATACcac                                                           <  2:8843142/117‑1 (MQ=255)
                                                              agtgataattttttttATTTGTTTATATTCAAAAAAAAAAAAAAAACAATCCCCAT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                       <  2:6243426/119‑1 (MQ=255)
                                                                tGATATTTTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCAC‑‑ATTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                       <  1:1751075/128‑1 (MQ=255)
                                                                 tttatatttttttattttttTAACTTAAAAAATAAAAAAAAACAAAACCCATAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                                      <  1:1026212/124‑1 (MQ=255)
                                                                 tataaatttttttatttttttATATTAAAAAATAAAAAAAAAAAAAACCCATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                                     <  1:9574356/123‑1 (MQ=255)
                                                                  ttatatttttttattttttTATAATAAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                                     <  1:5219978/125‑1 (MQ=255)
                                                                      atttttttatttttttTATTTTAAAAAAAAAAAAAAAAAATACC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                                <  2:7366834/128‑1 (MQ=255)
                                                                      aTTTTTTTAATTTTTTATCATAAAAAAAAAAAAAAAAAAAAACACC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                                 <  1:2810962/128‑1 (MQ=255)
                                                                       tttttttatttttttttattAAAAAAAAAAAAAAAAAAAAAAACCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                                 <  2:8089018/128‑1 (MQ=255)
                                                                        ttttttATTTTTTTAAAATAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                              <  1:2195509/128‑1 (MQ=255)
                                                                         ttttttttttttTATATTTAAAAATAAAAAAAAAAAAATACCCATT‑‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                             <  1:536669/128‑1 (MQ=255)
                                                                         tttttAATTTTTTAAATTAAAAAATAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                             <  1:8325962/128‑1 (MQ=255)
                                                                          ttttATTTTTTTTAATTAAAAAATAAAAAAAAAAAAATACACTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                                          <  1:3585271/128‑1 (MQ=255)
                                                                          ttttATTTTTTTATCTTAAAAAATAAAAAAAAAACAATCCCC‑‑TAAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                             <  1:7742168/128‑1 (MQ=255)
                                                                          ttttATTTTTTATATTTAAAAAAAAAAAAAAAAAAAAAACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                             <  1:1315819/128‑1 (MQ=255)
                                                                           ttttttttttttTATTAAAAAATAAAAAAAAAAAAATTCCATTT‑‑AAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                                          <  2:8216592/128‑1 (MQ=255)
                                                                            ttATTTTTTTATATTAACAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTGAAATTCCCTATTATACCACATTTTATTTTTAAAATa                                          <  1:7235383/128‑1 (MQ=255)
                                                                               ttttttTATATTAAAAAAAAAAAAAAAAAAAAAACACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                                      <  1:4410994/128‑1 (MQ=255)
                                                                                ttttttATCTTCAAAAATAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                                    <  1:7321251/125‑1 (MQ=255)
                                                                                   tttATATTAACAAAAAAAAAAAAAAAAAATACCCTTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                                  <  1:7972019/128‑1 (MQ=255)
                                                                                     tttatttaataaaaaaaaaaaaaaaaaATACCATT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTg                                 <  1:3082460/117‑1 (MQ=255)
                                                                                     tttaaataaaaaaaTAAAAAAAAAAAAAAACCATTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                                    <  1:6543852/117‑1 (MQ=255)
                                                                                         aattaaaaaaaaaaaaaaaaaCAATCCAATTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                                <  1:9384989/121‑1 (MQ=255)
                                                                                          atttaaaaaTAAAAAAAAAAAAATATCATTT‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACat                             <  1:50582/122‑1 (MQ=255)
                                                                                          aataaaaaaaaaaaaaaaaaCAATCAC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                              <  1:1962441/122‑1 (MQ=255)
                                                                                           ttaaaaaaaaaaaaaaaaaaaATAACCATAAAAATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                              <  1:2581002/123‑1 (MQ=255)
                                                                                           ttCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAATCTATTTATAAAAATAACATTATAAAACAAAATTTATTTTTAAAAAAAGatttagaata                             >  1:8447852/1‑120 (MQ=255)
                                                                                            ttcaaaaTAAAAAAAAAAAAAAAACCCTATAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAATATATTGACa                              <  1:580915/124‑1 (MQ=255)
                                                                                            taaaaaaTAAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata                         <  1:8101395/124‑1 (MQ=255)
                                                                                               aaaaaaaaaaaaaaaCAATCCC‑‑ATAAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata                         <  1:4146289/127‑1 (MQ=255)
                                                                                                 aaTAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAA‑‑AAAAACCAACTCTATTTTTTAAAAATACCTTTTTAACCCAATTTTTTTTTTAAATAAAGATATTGTaataattttta                     >  2:7594613/1‑123 (MQ=255)
                                                                                                  aTAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAACAA‑‑AAAACAAAACTAAATTTATAAAATTACCCTTTTAAACCCCATTTTATTTTTAAAATAATATATTGACataataatatt                    >  2:7301414/1‑123 (MQ=255)
                                                                                                  aTAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAATATATTTATTAAAATAAAATATTATTCCAAATTTATTTTTTAAAAtaaaataataaaataaatatta                     >  2:8510320/1‑122 (MQ=255)
                                                                                                  aTAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAA‑‑AAAAACAAAATATATTTATTAAAATACCATATTATACAAAATTTTATTTTAAAAATAAAATATTTAAAAAatatataa                    >  1:8836476/1‑127 (MQ=255)
                                                                                                   tAAAAAATAAACAATTCCC‑‑TTAAAAATTTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACAACATTTTATTTTTAAAATAAGATATTGACATAatatata                     <  1:8756373/128‑1 (MQ=255)
                                                                                                   tAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAA‑‑AAAAACAAACAATAATTATTAAAATAAAATAATAAAAAAAATTTTATTTTTAAAATAAGAAATTTAAATTatatatata                   >  1:8259796/1‑127 (MQ=255)
                                                                                                   aaaaaaaaaaaaaaATTACATTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatat                        <  1:3949783/126‑1 (MQ=255)
                                                                                                             aaCAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACAAAAAACATACAAATGTGTTAAAATTACCGATTTAATCCAATTTATATTTTTAAAAAAATAAATTTATATAatataaataaattataat          >  2:9040892/1‑118 (MQ=255)
                                                                                                              aaaaaatacccttAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAata              <  1:2989329/117‑1 (MQ=255)
                                                                                                                aaaataccattaaaaattaaaaaAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatata            <  1:3372053/119‑1 (MQ=255)
                                                                                                                    acaatttAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATataa       <  1:6737616/124‑1 (MQ=255)
                                                                                                                       ccttaaaaattaaaaaAAAAAAAAAAAAAAAAATAAAAAAAAACTATATTAATTAAAATAAAATATTATAACACATTATTTTATTAAAAAAATAATATTACATAATTtttataaaatttataaaaaaa    >  2:2822471/3‑125 (MQ=255)
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AATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTA  >  CP016816/202369‑202614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: