Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,801 A→G intergenic (‑110/+61) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,8010AG98.2% 15950.4 / NA 4377intergenic (‑110/+61)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (14/3);  major base G (1988/2282);  minor base T (55/24);  total (2068/2309)
Fisher's exact test for biased strand distribution p-value = 5.35e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4846 total aligned reads displayed.

AAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484675‑484925
                                                                                                                               |                                                                                                                            
  aTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCaa                                                                                                                           >  1:1153250/1‑128 (MQ=255)
     gTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTa                                                                                                                        >  2:7449463/1‑128 (MQ=255)
     gTCAAAACTCATAGATTTAATCCTTATATAGTTGACCAAAAAATAAGATAGAGAATAGCAGAATCTCTTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTa                                                                                                                        <  1:3333938/128‑1 (MQ=255)
      tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                                       <  1:6572858/128‑1 (MQ=255)
       cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                                      <  2:8169771/128‑1 (MQ=255)
        aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                                     <  1:5262165/128‑1 (MQ=255)
         aaaCTCATAGATTTAATCCTTTTATAGTTGCCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAAGTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                    >  2:4525062/1‑128 (MQ=255)
         aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                    >  2:8309999/1‑128 (MQ=255)
         aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                    >  1:1509207/1‑128 (MQ=255)
          aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                                   >  1:5844151/1‑128 (MQ=255)
          aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                    <  1:6790917/127‑1 (MQ=255)
           aCTCATAGATTTAATCCTTTTATAGTTGACAAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                                  >  2:6198273/1‑128 (MQ=255)
            cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAATATATAGCATAGAAGAATCTCTTAACAACTAAACTAAACTCGATAATATAATTATATTTTTTTTTAATATAAAAACCAATTAAaaacac                                                                                                                 >  1:8723536/1‑128 (MQ=255)
            cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGCAGCTCTTAAAAACGAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAccacac                                                                                                                 >  2:7809714/1‑128 (MQ=255)
             tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGATCTTAACAACTAAACTAAACTCTAATATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                                >  1:3558826/1‑128 (MQ=255)
              cATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaa                                                                                                               >  2:6541514/1‑128 (MQ=255)
                  gATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                                           <  1:4404814/128‑1 (MQ=255)
                    tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                         <  2:797102/128‑1 (MQ=255)
                      tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAacacac                                                                                                       >  2:1622020/1‑128 (MQ=255)
                      tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                       >  1:9171198/1‑128 (MQ=255)
                      tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                       >  2:510155/1‑128 (MQ=255)
                       aaTCCTTTTATAGTTGACCAAAAAATCAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                                      <  1:8548074/128‑1 (MQ=255)
                          ccTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                                   <  1:4758005/128‑4 (MQ=255)
                          cattttATAGTTGACCAAAAAATAATATAGAGCATAGAAGAAGCTCTTAACAACTAAACTACACTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAt                                                                                                   <  1:6956254/126‑1 (MQ=255)
                             tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAACAGCAATAAAACACACAAAAACTCACCCAGTAt                                                                                                >  2:566316/1‑128 (MQ=255)
                             tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                                >  2:5486366/1‑128 (MQ=255)
                             tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                                >  1:6393581/1‑128 (MQ=255)
                              ttATAGTTGAACAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                               <  2:3509015/128‑1 (MQ=255)
                                ataGTTGAACAAAAAATAAGATAGATCATAGCAGTAGCTCTTTACAAATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAATAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                             <  1:545754/128‑1 (MQ=255)
                                  aGTTGACCAAAAAATAAGATAGAGCTTAGCAGAAGCTCTTAACAACTAAACTTCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAATATGTTGa                                                                                           >  2:914478/1‑128 (MQ=255)
                                  aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                           <  1:7780706/128‑1 (MQ=255)
                                     tGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTATTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAACCATTATGGTGAGta                                                                                        >  2:2477971/1‑127 (MQ=255)
                                     tGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAGGCAATTAAACACACAAAAACTCACCCATTATGGTGAGtt                                                                                        >  1:7603336/1‑128 (MQ=255)
                                       aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                                      <  1:2021452/128‑1 (MQ=255)
                                        ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                                     >  1:7421787/1‑128 (MQ=255)
                                           aaaaaTAAGATATAGAATAGCATAAGCTCTTAAAAACTAAACTACACTAGATTATTTAATTATATTTTTTATTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  2:6376350/128‑1 (MQ=255)
                                           aaaaaTAAGATAGAGCATAGCAGTAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTATACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  2:9000494/128‑1 (MQ=255)
                                           aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  2:7242536/128‑1 (MQ=255)
                                           aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  2:1966194/128‑1 (MQ=255)
                                              aaTAAGATCGAGCATATCAGAAGCTATTAACAACTAAACTACAGTTGATTATTTAATTTTATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatta                                                                               <  2:1795971/128‑1 (MQ=255)
                                              aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  1:9374640/125‑1 (MQ=255)
                                                 aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACGCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                            >  2:2325450/1‑128 (MQ=255)
                                                  aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                           <  1:2664745/128‑1 (MQ=255)
                                                  aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAATAAACACACAAAAACTCACCCATAATGTTGAGTTTAAAAATTATTCa                                                                           >  2:8821879/1‑128 (MQ=255)
                                                   gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                                          <  1:2823973/128‑1 (MQ=255)
                                                    aTAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                                         <  1:2999671/128‑1 (MQ=255)
                                                      agagCATAGCAGAATCTCTTAAAATCTAAACTACACTCGATTTTTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                                       <  2:9515501/128‑1 (MQ=255)
                                                      agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                                       <  1:6046064/128‑1 (MQ=255)
                                                        agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                      <  1:3187654/128‑1 (MQ=255)
                                                         gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                                    >  2:3316412/1‑128 (MQ=255)
                                                         gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                                    >  2:2636311/1‑128 (MQ=255)
                                                            tatcAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                                 <  1:5084147/125‑1 (MQ=255)
                                                             atcAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCTCCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                                <  1:1848840/126‑1 (MQ=255)
                                                             aGCAGAAGCTCTTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                                <  2:1159949/128‑1 (MQ=255)
                                                              gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                               <  1:8863718/128‑1 (MQ=255)
                                                              gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                               >  1:2313987/1‑128 (MQ=255)
                                                              gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTAAATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                               <  2:9325342/128‑1 (MQ=255)
                                                                 gaaacgattAACAACTAAACTACACTCGATTAGTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                            <  2:2991264/121‑1 (MQ=255)
                                                                 gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                            <  1:8405166/128‑1 (MQ=255)
                                                                 gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACAAACAAAAACTCACCCATTATGGTGATTTTATAAATTATTAAAAAATTTGATTAgtt                                                            >  2:4367594/1‑128 (MQ=255)
                                                                  aaGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAtttt                                                           >  1:7997637/1‑128 (MQ=255)
                                                                   aGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTAATCAAAAATTATATTATTTTa                                                          >  2:8468578/1‑128 (MQ=255)
                                                                     ctcaTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                                        <  2:7052909/128‑1 (MQ=255)
                                                                      tctTATGAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                                       <  1:4905338/128‑1 (MQ=255)
                                                                      tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                                       <  1:5646287/128‑1 (MQ=255)
                                                                        ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                                     >  1:8210147/1‑128 (MQ=255)
                                                                         tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                         >  1:9542455/1‑75 (MQ=255)
                                                                            caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                                 >  2:38792/1‑128 (MQ=255)
                                                                              actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               >  1:4124606/1‑128 (MQ=255)
                                                                               ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAAAACACAAAAACTCACCCATTATGCTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                              <  1:6221040/128‑1 (MQ=255)
                                                                                  aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                           <  1:4554740/128‑1 (MQ=255)
                                                                                  aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                           <  1:2154500/128‑1 (MQ=255)
                                                                                  aactaCACTCGATTAATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAATATCTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                                           <  2:6027786/128‑2 (MQ=255)
                                                                                  aactaCAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTGACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                           <  2:8665767/128‑1 (MQ=255)
                                                                                  aactaAAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                                           <  2:4944898/128‑2 (MQ=255)
                                                                                   actaCACTCTATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAATCACACAATAACTCACCAATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcc                                          <  1:7128028/128‑1 (MQ=255)
                                                                                    ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                                         <  1:7257088/128‑1 (MQ=255)
                                                                                      acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTATAAATTATTCAAAAATTTGATTATTATAAATCAATTTAAGCTCCAGc                                       >  2:4738685/1‑128 (MQ=255)
                                                                                      acaatagATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAAATCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                       <  1:3733203/122‑1 (MQ=255)
                                                                                       cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                                      >  2:7629151/1‑128 (MQ=255)
                                                                                        acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                                     <  1:9041984/128‑1 (MQ=255)
                                                                                        acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                                     >  2:421482/1‑128 (MQ=255)
                                                                                        acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                                     >  2:5826621/1‑128 (MQ=255)
                                                                                          tCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                                   <  2:4057952/128‑1 (MQ=255)
                                                                                             attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                                <  2:214158/128‑1 (MQ=255)
                                                                                              ttattTAAATATATTTTTTTTTAATATAAGAAGCAAGTAACCACACAAAAACTCACCCATTATGGGTAGTTTAAAAATTAATCAAAAATTTGATTATATTAAATCATTTGAAGGACAAGATTCAACTa                               >  2:6853549/1‑128 (MQ=255)
                                                                                                attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                             <  2:1481548/128‑1 (MQ=255)
                                                                                                 tttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCATCTTCAACTAAtt                            >  2:2804888/1‑128 (MQ=255)
                                                                                                 tatAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAtt                            <  1:1699355/126‑1 (MQ=255)
                                                                                                  ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  1:1321382/128‑1 (MQ=255)
                                                                                                     aTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGc                        <  1:2485162/128‑1 (MQ=255)
                                                                                                      ttATATTTTTTTTTAATATAAAAATCAATTTAACACATAAAAACTCTCCCATTATTGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt                       <  2:3165814/128‑1 (MQ=255)
                                                                                                            ttttttttaATATAAAAAGCAATTAAACACACAAAAAATCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt                 <  2:6713684/128‑1 (MQ=255)
                                                                                                            ttatttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATAATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt                 <  1:390456/125‑1 (MQ=255)
                                                                                                             tttttttaATATAAAAAGCAATTAATCACACAAAAACTCACACATTATGGTGAGTTTAAAAATTTTTGAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt                <  2:3705062/128‑1 (MQ=255)
                                                                                                             tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt                >  2:1320580/1‑128 (MQ=255)
                                                                                                              ttttttaATATAAAAAGCTATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttc               <  1:4263559/128‑1 (MQ=255)
                                                                                                                     aatataaaaagAAATTAAACACACAAAAACTGACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGc         <  2:5293227/123‑1 (MQ=255)
                                                                                                                      atataaaaagCAATTAAAGACACAACAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGct        <  2:1026398/124‑1 (MQ=255)
                                                                                                                          aaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttctt    >  1:1001201/1‑128 (MQ=255)
                                                                                                                               |                                                                                                                            
AAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484675‑484925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: