Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA98.6% 10672.3 / 102.4 3507intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (2179/1254);  minor base . (10/39);  total (2192/1315)
Fisher's exact test for biased strand distribution p-value = 1.29e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3602 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATA  >  CP016816/165097‑165350
                                                                                                                               |                                                                                                                                
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                >  2:8833879/1‑128 (MQ=255)
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                <  1:5657467/128‑1 (MQ=255)
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                >  1:2772406/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               <  2:2100883/128‑1 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAAaat                                                                                                                               >  2:6875608/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTATTTGTATATGCAAATTTTTAAAAaat                                                                                                                               >  1:3602790/1‑128 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                            >  1:4664312/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:234622/128‑1 (MQ=255)
         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTAACTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                       <  2:7651782/128‑1 (MQ=255)
          catactttactAGTTGTTAATATTTAGGTTCATTAATTTATCGAACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      <  2:4259125/126‑1 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCTAATTTTTAAAAAATTAATTTGaa                                                                                                                      >  2:5083730/1‑128 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      <  2:7089385/128‑1 (MQ=255)
            taattttctAGTTGATAATATTTAGTTTTATTAATTTATAGAACTTGTATGAATTAGTAAGTAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAATATTAATTTGAACt                                                                                                                    <  1:50026/125‑1 (MQ=255)
             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                   <  1:5539146/128‑1 (MQ=255)
             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                   <  2:7957243/128‑1 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCTCACTTGTATGAATTAGTAAGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATATTCAAATTATTAAAAAATTAATTTTAACtttt                                                                                                                 >  2:6111681/1‑126 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:7827689/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               >  2:9461505/1‑128 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                              <  1:4510897/128‑1 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                              >  1:9354462/1‑128 (MQ=255)
                        tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                        >  2:9324344/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                              >  1:8052718/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                              >  2:193613/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                              >  1:341763/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCTAATTTTTAAAAATTTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                            >  1:6252304/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                           >  2:3500235/1‑128 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                        >  1:8361765/1‑128 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                       >  2:3626517/1‑128 (MQ=255)
                                          ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                      >  2:5939254/1‑128 (MQ=255)
                                           tAATTTATAGCACTTGTTTTAATTATTAATAAAATAAAAAAAATATATTTTTATAAATTTTTTGTATATTCAAATTTTTAAAAAAATAAATTTAACTTGTAAAAAAAACCttttttttttttttttta                                                                                     <  1:3759194/128‑2 (MQ=255)
                                            aaTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttat                                                                                    >  2:1138313/1‑126 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttaaaattg                                                                                  >  1:1883980/1‑121 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      <  2:49112/92‑1 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTatat                                                                                  >  2:2501753/1‑128 (MQ=255)
                                               ttATCGCAATTGTAATAAATACTAATAAACTAAAACAATTATATTTTTATATATTTTTTTTTTATTCAAATTTTTAAAAAAATAATTTTAACTTGTAAAAAATAGCCTTTTTTTTTTTTTTTtataat                                                                                 <  1:4282830/128‑3 (MQ=255)
                                                 aTCTCACTTTTATGAATTATTAATAAAATAAAACAATTATCTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAAATAATTTTAACATTTAAAAAATATCTTTTTTTTTTTTTTTTtataatgg                                                                               <  1:5151175/128‑5 (MQ=255)
                                                  tCGCAATTGTTTTAATTTTTAAACAACTAAAAATATTTTTATTTTATTTATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAAAACCCTTTTTTTTTTTTTTTTTATAATgg                                                                             <  1:2028310/128‑1 (MQ=255)
                                                     cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAAtttaaa                                                                          >  1:5851494/1‑123 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTATTaatattaaaaaa                                                                         >  2:1317354/1‑120 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttattatttaaata                                                                         >  2:525839/1‑116 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAattt                                                                       >  2:2812651/1‑126 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAAtata                                                                      >  1:6784440/1‑128 (MQ=255)
                                                        ttGAATGAATTATTAAGAAACTAAAAATATTATCTTTATATATATTTTTTGTATTTGCAAATTTTTAAAAAAATAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                      <  1:920569/128‑1 (MQ=255)
                                                          gtatttaTTAGTAAGAAAATAAAAAAATTTTTTTTTTATTTATTTTTTTTATTTGCAAATTTTTAAAAAAATAATTTTTAATTTTAAAAAAAAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                    <  1:6418932/122‑1 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAAttaataaaaaa                                                                     >  1:4105027/1‑118 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAATGAAAataaata                                                                    >  2:7426910/1‑124 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATaat                                                                    >  2:8091607/1‑127 (MQ=255)
                                                            atgtttttttaataaAATAAAAATTTTATCTTTTTATATATTTTTTTTTTATTCAAATTTTTAAAAAATTTATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                  <  2:8443880/119‑1 (MQ=255)
                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATATTGAATTATaatt                                                                   >  1:1636488/1‑126 (MQ=255)
                                                             tGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAATTATAAAAATAAAAtt                                                                  >  1:6603588/1‑128 (MQ=255)
                                                              taataattaataaAATAAAAAATTTATTTTTTTTTTTATTTTTTTTTTATTCAAATTTTTTAAAAATTAATTTTTAATTTTTAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAttt                                                                 <  2:824301/121‑1 (MQ=255)
                                                               aatttttaataaAATAAAAATTTTATATTTTTTTATATTTTTTTTATATTTAATTTTTTAAAAAATTAATTTTAACTTGTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                               <  1:4831823/122‑1 (MQ=255)
                                                               aaatattAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                               <  1:9497891/122‑1 (MQ=255)
                                                                attattAATAAAATAAAACTATTTTCTTTTTATATATTTTTTGTATATTTAAATTTTTAAAAAATTTATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                              <  1:7018933/128‑1 (MQ=255)
                                                                aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGTAAATATAAATTTTa                                                               >  2:3817034/1‑128 (MQ=255)
                                                                  tactAAGAAAATAAAACTATTTTCTTTTTTTATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                            <  2:180756/125‑1 (MQ=255)
                                                                     taattaactataagtaTTTTTTTTTTTTTTATTTTTTGTTTATTAAAAATTTTAAAAAATTTAATTTTATTTTTAAAATAT‑‑‑TGGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                        <  1:7207361/123‑1 (MQ=255)
                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTAAttt                                                        >  1:8529419/1‑128 (MQ=255)
                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTTTAAATATAATTTTTATTTAtttttt                                                     >  1:5345760/1‑126 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGGAATTATAAATTTTAGTtttttttt                                                     >  2:900162/1‑126 (MQ=255)
                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTAtttttt                                                     >  1:174774/1‑126 (MQ=255)
                                                                          aaataaaacttTTATTTTTTTTTATTTTTTTTTTTTATTAAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                      <  1:7398736/125‑1 (MQ=255)
                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTaattttttttttt                                                   >  1:3883994/1‑116 (MQ=255)
                                                                            ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGTAATATAAATTTTAGtttttttttttta                                                 >  2:6311111/1‑125 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTATTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTAAGTTATTATGTTTTCt                                                >  2:6435132/1‑128 (MQ=255)
                                                                             aaaaactaTTTTATTTTTATATATTTTTTGTTTTTTTAAATTTTTAATAAATTTATTTGAAATTTTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                  <  2:8619128/127‑1 (MQ=255)
                                                                                aactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATTAAAATTTTATTTATTTTTTTTTATaa                                              >  1:1151848/1‑128 (MQ=255)
                                                                                 atttttttttttttAGTTATTTTTTTTTTTTTAAAATTTTTTAAAAATTAATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                              <  2:4007396/119‑1 (MQ=255)
                                                                                 aatattttatttTTTTATTTTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAAAAG‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                             <  2:5714656/126‑1 (MQ=255)
                                                                                 aatattatATTTTTTTATATTTTTTTTTTATTAAAATTTTTTAAAAATTAATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                              <  2:6628503/126‑1 (MQ=255)
                                                                                  ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTATTAAAATTTAAATATAAATTTTAATTAtttatttgaataata                                            >  1:328400/1‑116 (MQ=255)
                                                                                    attattTTTTTATTTATTTTTTTTTTTTTTAAATTTTTTAAAAATTTATTTTAAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGt                                            <  1:7768504/128‑1 (MQ=255)
                                                                                     tttttttttttTATATTTTTTTTTTTTTATAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                         <  1:760103/123‑1 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTTATAATTTAAAAAAAAATTTTAGTTATTTTTTTTTATAATTa                                           >  1:983531/1‑128 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAAAATAAATTTTAGTTATtttttttaataattttc                                         >  2:2640612/1‑117 (MQ=255)
                                                                                         ttttttataaatttttttttATATGAAAATTATAAAAAATTAAATTTAAAATTTAAAAAAAA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaa                                    <  1:2198794/116‑1 (MQ=255)
                                                                                          tttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATTTATTTTTTTTTATAATTAtaaaaa                                     >  2:2298344/1‑123 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTAAAATATAAATTTAAGTTATTTTTTTTTATAATTATATaaaaaa                                  >  2:4774288/1‑128 (MQ=255)
                                                                                            tttttttatTTTTTTTATTTGAAATTTTTTAAAAAATTAATTTTAAATTTTTAAAAAAAGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaa                                   <  2:9161283/122‑1 (MQ=255)
                                                                                              tAGATAATTTTTTTTATTGGAAAATTTTAAAAATTTTATTTTAAATTTTAAAAAATTGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                              <  2:3142599/128‑1 (MQ=255)
                                                                                               ttttatTTTTTTTATTTTAAAATTTTTTAAAAATTAATTTTAAATTGTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                              <  1:2944105/125‑1 (MQ=255)
                                                                                                  taatTTTTTGTATATTTAAATTTTTAAAAAATTTATTTGAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                             <  1:885506/126‑1 (MQ=255)
                                                                                                      tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTAATATTATGATAATAAAAATTTTTGATATTTTGTTTTCTAATTATATAAAAAATAAGaaat                          >  2:4385992/1‑127 (MQ=255)
                                                                                                       ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAAAATGGAAATAAAAATTTTAGTTATTTTGTTGTATTAGTATATAAAAAAATAAAAAgg                         >  2:7269120/1‑128 (MQ=255)
                                                                                                         tttttttatttaaaTTTTTAAAAAATTTATTTTTAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAATATCTAAAAAAATAGAAAGGa                        <  2:1557487/117‑1 (MQ=255)
                                                                                                         ttttataTTTAAATTTTTAAAAAATTAATTTTAAAATGTTAAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                      <  1:1290062/125‑1 (MQ=255)
                                                                                                         ttttataTGCAAATTTTTAAAAAATTAATTTGTACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTGTATAATTATATAAAAAAATATaaattaaa                      >  1:259478/4‑123 (MQ=255)
                                                                                                         tttgttaattgaaaTTTTTAAAAAATTAATTTGTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                      <  1:9031522/117‑1 (MQ=255)
                                                                                                         ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTATAATTATATAAAAAAATAGaaattaaa                      >  2:3301984/1‑123 (MQ=255)
                                                                                                          ttttaatttaaaaTTTTTTAAAAATTAATTTGAAATTTTAAAAAAT‑‑TTCTATTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                      <  2:2980372/118‑1 (MQ=255)
                                                                                                          tttataTTGAAATTTTTTAAAAATTAAATTTTTATTTTATAAAAAATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                       <  2:5443463/126‑1 (MQ=255)
                                                                                                                tttttaaTTTTTTAAAAATTAATTTTAAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                  <  1:8680318/123‑1 (MQ=255)
                                                                                                                  ggaaaTTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat                <  1:792616/126‑1 (MQ=255)
                                                                                                                   cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATTATTTT‑‑‑AATATAAATTTTATGTTTTTTTTTTTATTTTTATATAAAAAAATAAAAAAGAAAAAATATtttt            >  1:8217122/1‑128 (MQ=255)
                                                                                                                    aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTGTTTTAAAAGTTTATAAAAAAATATAAATGAAAAAATTTTg              >  1:1012470/1‑128 (MQ=255)
                                                                                                                    aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGTTATTTTGTTTAATAATTTAATAAAAAAATAAAAAGTAAAAAATAttttaa           >  2:4554221/1‑124 (MQ=255)
                                                                                                                          ttaaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTATGTAAATATAAATTTTATGTTTTTTGTTTTATAATTTTATAAAAAAATAGAAAGTAAAAAATATTTTTaaaat       >  1:3077312/3‑127 (MQ=255)
                                                                                                                          ttaaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTGTTGTATAATTATCTAAAAAAATATAAATTAAAAATATTTTTtaaatg      >  1:5958492/3‑126 (MQ=255)
                                                                                                                          ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTTTTTTGTTTTATAAGTATATAAAAAAATAGAAATTAAAAATTATTTTTAAAAGt      >  1:1418841/3‑128 (MQ=255)
                                                                                                                           taaaaaaTTAATTTTAAATTTTTAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa     <  1:6095142/127‑1 (MQ=255)
                                                                                                                               |                                                                                                                                
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATA  >  CP016816/165097‑165350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: