BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	281,616	C→T	Q100* (CAA→TAA)	JCVSYN2_01185 →	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	281,616	0	C	T	99.8%	14071.3 / NA	4409	Q100* (CAA→TAA)	JCVSYN2_01185	DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (2079/2318); minor base A (7/1); total (2090/2319)
Fisher's exact test for biased strand distribution p-value = 3.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4446 total aligned reads displayed.

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

 tGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAaataat                                                                                                                           >  1:784661/1‑128 (MQ=255)

 tGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAaataat                                                                                                                           <  2:9518874/128‑1 (MQ=255)

  gATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAtt                                                                                                                          >  2:1191455/1‑128 (MQ=255)

    ttCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTta                                                                                                                        <  1:2755890/128‑1 (MQ=255)

      cTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                      <  2:4000959/128‑1 (MQ=255)

      cTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                      <  1:2930137/128‑1 (MQ=255)

        aTTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaaa                                                                                                                    >  1:224480/1‑128 (MQ=255)

        aTTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaaa                                                                                                                    <  1:3099949/128‑1 (MQ=255)

             tAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGtt                                                                                                               <  2:6825084/128‑1 (MQ=255)

               aaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTa                                                                                                             <  2:9298164/128‑1 (MQ=255)

               aaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTAATAAAAAGTTTa                                                                                                             >  2:1576209/1‑128 (MQ=255)

                aaCTAGTAATTTATTATTTAATTTAGAGAAAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                            <  1:8374598/128‑1 (MQ=255)

                aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                            >  2:1733945/1‑128 (MQ=255)

                aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                            >  1:7157837/1‑128 (MQ=255)

                aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                            >  1:4234572/1‑128 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  2:4997237/128‑1 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  2:911125/128‑1 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTCACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           >  2:3506909/1‑128 (MQ=255)

                   tAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAag                                                                                                         >  2:7752960/1‑128 (MQ=255)

                    aGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAaga                                                                                                        >  2:8092028/1‑128 (MQ=255)

                    aGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAaga                                                                                                        <  2:3147779/128‑1 (MQ=255)

                    aGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTATAAAaga                                                                                                        >  1:6633100/1‑128 (MQ=255)

                     gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       <  2:2818428/128‑1 (MQ=255)

                       aaaTTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGt                                                                                                     <  2:8223825/128‑1 (MQ=255)

                          ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGTTGAt                                                                                                  >  2:6636789/1‑128 (MQ=255)

                          ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                  >  2:8437028/1‑128 (MQ=255)

                            attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                >  2:5168184/1‑128 (MQ=255)

                             ttattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtg                                                                                               <  1:5907088/128‑1 (MQ=255)

                                 ttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATATAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgatga                                                                                           <  1:3425274/128‑1 (MQ=255)

                                  taatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGaa                                                                                          <  1:7676321/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATATAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                        <  2:6113446/128‑1 (MQ=255)

                                       taGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAt                                                                                     <  1:4111356/128‑1 (MQ=255)

                                           gaCAAAATTACATTTAAATCAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAt                                                                                 <  2:2510858/128‑1 (MQ=255)

                                           gaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAt                                                                                 <  1:5706557/128‑1 (MQ=255)

                                           gaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAt                                                                                 <  2:6988501/128‑1 (MQ=255)

                                             cAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGa                                                                               <  2:101946/128‑1 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGTTGATTTGATGAAAGTATTAATGAt                                                                              >  1:143568/1‑128 (MQ=255)

                                               aaaTTACATTTAAAACAAAAACATGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGTAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATc                                                                             <  1:6091904/128‑1 (MQ=255)

                                               aaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATc                                                                             >  1:160946/1‑128 (MQ=255)

                                               aaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATc                                                                             >  2:7931671/1‑128 (MQ=255)

                                                aaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCg                                                                            >  1:3806820/1‑128 (MQ=255)

                                                  ttACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGga                                                                          <  1:5564628/128‑1 (MQ=255)

                                                      aTTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATg                                                                      >  2:2146688/1‑128 (MQ=255)

                                                      aTTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATg                                                                      <  1:818624/128‑1 (MQ=255)

                                                       tttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGt                                                                     <  1:3962379/128‑1 (MQ=255)

                                                        ttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGtt                                                                    <  1:8667400/128‑1 (MQ=255)

                                                        ttAAAACAAAAACAGGATTATCTAAATTCTCTAATTAATAAAATTGAAACAACTCAAATAGATGTAAAATAATTTATAAAAAGGTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGtt                                                                    <  2:233295/128‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  <  1:2079427/128‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  2:5950637/1‑128 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATg                                                                >  2:6271402/1‑128 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATAAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATg                                                                >  2:1391469/1‑128 (MQ=255)

                                                             acaaaaacaGGAATAACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGACGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGc                                                               <  2:5365270/128‑1 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGATATGTTTATGaaaa                                                            >  2:2981807/1‑128 (MQ=255)

                                                                 aaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCaaaa                                                           <  1:9250772/128‑1 (MQ=255)

                                                                  aacaTGAATTACTAAATTCTCTAATTAAAAAAATTGAAACAAATCCAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAAc                                                          <  1:5186917/128‑1 (MQ=255)

                                                                    caGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTGATGATCGGAGATGTTTATGCAAAACCg                                                        >  1:3269966/1‑128 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCgg                                                       >  1:8536029/1‑128 (MQ=255)

                                                                      ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCggg                                                      >  2:3310944/1‑128 (MQ=255)

                                                                      ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAAAAATTTATAAAAAGTTTAAAAGAGGTGATTTGAAGAAACAATTAAAGATCGGACATGTTTATGCAAAAccaga                                                      >  2:7510943/1‑125 (MQ=255)

                                                                        aattccTATTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAATAATAATTTATAAAAAGTTTAAAAGAGGTCATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGaa                                                    <  1:6283320/123‑1 (MQ=255)

                                                                          ttACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAaga                                                  <  2:4341915/128‑1 (MQ=255)

                                                                           tACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGa                                                     >  2:3827175/1‑124 (MQ=255)

                                                                           tACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAagag                                                 <  1:8845763/128‑1 (MQ=255)

                                                                           tACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAagag                                                 <  1:8024663/128‑1 (MQ=255)

                                                                              tAAATTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGAGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaaa                                              <  2:4816114/128‑1 (MQ=255)

                                                                                aTTTCTCTAATTAATAAAATTGAAACAAAACAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTTATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATg                                            >  2:4483184/1‑128 (MQ=255)

                                                                                  ttCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCt                                          <  1:4721318/128‑1 (MQ=255)

                                                                                      ctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGAAAAACGGGTAAGAGAAAtcatataa                                      >  2:4177647/1‑121 (MQ=255)

                                                                                       taattaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaa                                     >  2:3650085/1‑128 (MQ=255)

                                                                                        aattaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaa                                    <  1:1002057/128‑1 (MQ=255)

                                                                                        aattaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaa                                    <  1:2603929/128‑1 (MQ=255)

                                                                                          ttaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAAc                                  <  2:1917991/128‑1 (MQ=255)

                                                                                           taataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAGCGGAGATGTTTATGCAAATCCGGGTAGAGAAATGGTAGATAAACa                                 >  2:6452604/1‑128 (MQ=255)

                                                                                            aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                <  1:6796614/128‑1 (MQ=255)

                                                                                            aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                >  2:7359230/1‑128 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:6452604/128‑1 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:1281323/128‑1 (MQ=255)

                                                                                              taaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCTGGAAGAGAAATGCTAGAAAAACAttt                              >  2:5575292/1‑128 (MQ=255)

                                                                                              taaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAttt                              <  2:7151859/128‑1 (MQ=255)

                                                                                              taaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACAGGGAAGAGAAAAGCTAGAAAACCAttt                              >  1:5030415/1‑128 (MQ=255)

                                                                                                aaaTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaa                            <  1:9415720/128‑1 (MQ=255)

                                                                                                    tGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTCTGAATACCCTGTAAGATAAATGCTAGAAAAACATTTaaaaaa                        >  2:8562494/1‑128 (MQ=255)

                                                                                                       aaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAatat                     >  1:9033197/1‑128 (MQ=255)

                                                                                                         caaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttg                   <  1:3595542/128‑1 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  <  1:1141457/128‑1 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  <  1:2440423/128‑1 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATTCAAAAACGTGAAGAGAAAGGCTAGATAAACATTTAAAAAATAttgtgg                >  1:6983627/1‑126 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                >  1:4571719/1‑128 (MQ=255)

                                                                                                              caaatAGATGAAAAATAATTTATAAAAAGGTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGat              <  2:89187/128‑1 (MQ=255)

                                                                                                                  tAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCATAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaa          >  1:730099/1‑128 (MQ=255)

                                                                                                                   aGATGAAAAATAATTTCTAAAAAGTTTAAAAGAGGTGATTAGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaa         <  1:5371790/128‑1 (MQ=255)

                                                                                                                    gATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaat        >  2:2655929/1‑128 (MQ=255)

                                                                                                                     aTGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatc       <  1:4400821/128‑1 (MQ=255)

                                                                                                                      tGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatca      >  1:4869204/1‑128 (MQ=255)

                                                                                                                      tGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatca      >  2:8694245/1‑128 (MQ=255)

                                                                                                                      tGAAAAATAATTTATAAAAAGTTTAAAAGAGGGGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatca      <  2:4641247/128‑1 (MQ=255)

                                                                                                                       gAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTaatcaaaatcaa     <  2:5761061/128‑1 (MQ=255)

                                                                                                                       gAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaa     >  1:7468426/1‑128 (MQ=255)

                                                                                                                        aaaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  1:9567705/128‑1 (MQ=255)

                                                                                                                          aaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATa  <  1:1848144/128‑1 (MQ=255)

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`