Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,157 2 bp→TT intergenic (‑440/+402) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1570AT99.5% 11804.4 / NA 3752intergenic (‑440/+403)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (9/10);  new base T (2015/1715);  total (2025/1727)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1580AT98.9% 11452.3 / NA 3754intergenic (‑441/+402)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (7/35);  new base T (2018/1694);  total (2025/1729)
Fisher's exact test for biased strand distribution p-value = 9.44e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3905 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285
                                                                                                                              ||                                                                                                                                  
    taaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatt                                                                                                                                >  2:2175052/1‑128 (MQ=255)
      aattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttt                                                                                                                              >  2:2945556/1‑126 (MQ=255)
            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                        >  2:5113358/1‑128 (MQ=255)
            ttcatACTTTTAGTCTCATAAATCTAACTATCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                        <  2:446125/128‑1 (MQ=255)
              catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                      <  2:9533126/128‑1 (MQ=255)
               atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                     <  2:7496637/128‑1 (MQ=255)
                 aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                    >  1:4514834/1‑127 (MQ=255)
                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                 <  2:3789999/128‑1 (MQ=255)
                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                 >  2:7798056/1‑128 (MQ=255)
                    tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                <  1:5615984/128‑1 (MQ=255)
                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                               >  2:8917659/1‑128 (MQ=255)
                      tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAAATAttt                                                                                                              >  1:6965418/1‑128 (MQ=255)
                         tctcATAAATCTAAGTTTCTAAAATTATTCTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                           >  2:7647284/1‑128 (MQ=255)
                         gctcATAAATCTAAGTTTCTTAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTTATATATTATAAATCTACTAGCACTTTTTTAAAAAAATTTTAATTTTAATAACTGAATTATTATTc                                                                                                           <  1:7516924/127‑1 (MQ=255)
                           taaTAAATCTAAGTATCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                         <  1:7350826/126‑1 (MQ=255)
                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                       >  2:6667344/1‑128 (MQ=255)
                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTATATTAGTTGATATATTAGAAAACTACTATCACTTTTTAAAAAAATTATTAATTATAATAACTGAATAATTATTCaaaa                                                                                                       >  1:9498284/1‑127 (MQ=255)
                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                      >  1:9151592/1‑128 (MQ=255)
                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAAtt                                                                                                      >  1:131633/1‑128 (MQ=255)
                              tAAATCTAAGTTTCTAAAATTATATTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTATAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                      <  2:2019924/128‑1 (MQ=255)
                               aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTAAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                     <  2:7057754/128‑1 (MQ=255)
                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  1:7689999/128‑1 (MQ=255)
                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  2:5730508/128‑1 (MQ=255)
                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  2:9237605/128‑1 (MQ=255)
                                 aTCTAATTTTATAAAATTATATTATCATTTAAAATAATAATACTTTTATTAGTTTATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                   <  1:607985/128‑1 (MQ=255)
                                 aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATTCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTATAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                   <  1:642223/128‑1 (MQ=255)
                                  tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                  <  1:7141820/128‑1 (MQ=255)
                                     aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                               <  2:5551778/128‑1 (MQ=255)
                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                              <  1:3740599/128‑1 (MQ=255)
                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                              <  2:4224437/128‑1 (MQ=255)
                                       gTTTCTATAATTATTTTATCATTTAAAAATATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                             <  2:879404/128‑1 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  2:8453992/1‑128 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  1:8186797/1‑128 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAAATACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATAATTTTTCAAATTGTTAAattatt                                                                                           >  2:1123562/1‑128 (MQ=255)
                                          tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                          >  2:2339512/1‑128 (MQ=255)
                                           cTAAAATTATTTTTTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAATACTTCTAGCTCTTTTTTAATAAATTTTTTATTTTAATAACTGACTTATTTTTCAAATTGTTATATTAtttt                                                                                         <  1:5320877/128‑1 (MQ=255)
                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCTAATTCTTTAATTAtttt                                                                                         >  2:8647805/1‑128 (MQ=255)
                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                         >  1:8035574/1‑128 (MQ=255)
                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTTTTAAATAAtttt                                                                                         >  1:2471470/1‑128 (MQ=255)
                                            tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                        <  2:7962913/128‑1 (MQ=255)
                                             aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                       >  2:4953529/1‑128 (MQ=255)
                                               aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                     >  2:7224565/1‑128 (MQ=255)
                                                attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAAATATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                    >  2:6239358/1‑128 (MQ=255)
                                                aatattTTATCATTTAAAAAAATTATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTATTAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                    <  1:3597716/126‑1 (MQ=255)
                                                 ttattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                   >  1:3445959/1‑128 (MQ=255)
                                                  tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                  >  1:8539329/1‑128 (MQ=255)
                                                    ctcttacgatctaaaaaaTAATACTTTTATTAGTTGAAATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTATAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAtt                                                                                <  2:8142304/116‑1 (MQ=255)
                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                            <  1:6595639/128‑1 (MQ=255)
                                                         tCATTTAAAAAAATAATACTTTTATTTGTTGATATTTTAGAAAACTACTAGAACTTTTTTAATAAATTTTTAATTTTAATATCTGAATTATTTTTCATATTGTTAAATTATTTTCAAATCATTATCTa                                                                           <  1:6277946/128‑1 (MQ=255)
                                                         tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                           <  1:5784820/128‑1 (MQ=255)
                                                           aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         >  1:3098227/1‑128 (MQ=255)
                                                            tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                        >  2:5991290/1‑128 (MQ=255)
                                                            tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                        >  1:870299/1‑128 (MQ=255)
                                                                 aaaaaTAATACTTTTATTAGTAGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                   <  2:3600157/128‑1 (MQ=255)
                                                                    aataatACTTTTATTACTTGATAAATTAGAAAACTACTAGCACTTTTTTAAAAAATTATTAATTATAATAACTGAATTATTTTTCAAAATGTTAAAATATTTTCAAATCATTATCTATTAATCAAttt                                                               <  2:1319072/128‑1 (MQ=255)
                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                    <  2:4808366/75‑1 (MQ=255)
                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                              >  1:2801454/1‑128 (MQ=255)
                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                              >  2:9386231/1‑128 (MQ=255)
                                                                        ataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATg                                                           >  1:1004074/1‑128 (MQ=255)
                                                                         taCTTTTATTATTTGATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTAttc                                                          >  1:8878456/1‑126 (MQ=255)
                                                                          aattttATTAGTTTATATATTATAAAAATAATAGAAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTTTTAAATTATTTTCAAAACATTATCTATTAATCAATTTTATGCt                                                         <  1:1459132/126‑1 (MQ=255)
                                                                            ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTATATTCTCg                                                       >  1:5957344/1‑128 (MQ=255)
                                                                            ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATTTTATGCTCg                                                       >  1:4983462/1‑128 (MQ=255)
                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                               <  1:9316495/119‑1 (MQ=255)
                                                                              ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCt‑‑tt                                                   >  1:1791325/1‑128 (MQ=255)
                                                                              ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tt                                                   >  1:19751/1‑128 (MQ=255)
                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                <  1:2942098/116‑1 (MQ=255)
                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCt‑‑ttt                                                  >  1:4346041/1‑128 (MQ=255)
                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttt                                                  >  1:3265946/1‑128 (MQ=255)
                                                                                  tatattctatatTAGAAAACAAATAACACTTTTTTAAAAAATTTTTAAATTTAATAACTTAAATATTTTTCAAATTGTTAAAATATTTTCAAAACAATATCTATTAAACAATTTTATGCTCG‑‑tttttt                                               <  2:3990349/121‑1 (MQ=255)
                                                                                  tacttGATAAATTATAAAACTACTATGAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATAATATATAAATCAATTTTATGCTCG‑‑tttttt                                               <  1:4852142/125‑1 (MQ=255)
                                                                                   aGTTTATATATTATAAAAATAATATTAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTAAAATTTTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttt                                              <  1:3903137/128‑1 (MQ=255)
                                                                                   aGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                              >  1:4665514/1‑83 (MQ=255)
                                                                                     ttGTTATATTTGAAAAATAATTGAAATTTTTTAAAAAATTTTTAATTTTAATAATTTTATTATTTTTAAAATTGTTAAATTTTTTTAAAATAATTATATTTTAATAAATTTTATGCTCG‑‑ttttttttt                                            <  2:6878262/128‑1 (MQ=255)
                                                                                       gATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttttt                                          >  1:5172099/1‑128 (MQ=255)
                                                                                         tatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttttttt                                        >  1:2674144/1‑128 (MQ=255)
                                                                                          atatTATAAAAATAATATTAATTTTTTAAAAAAATTTTAATTTTAAAAAATGAATTATTTTTAAAATTTTTAAATTATTTTAAAATCAAAATATATTAATAAATTTTATTCTCG‑‑tttttttttttttt                                       <  1:9011980/128‑1 (MQ=255)
                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                        <  2:276932/82‑1 (MQ=255)
                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                              <  1:5446347/92‑1 (MQ=255)
                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttt                                       >  2:3162497/1‑128 (MQ=255)
                                                                                            atTTGAAAACTACTAGAAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTTAAATTTTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTATGCGCG‑‑tttttttttttttttt                                     <  2:8292298/128‑1 (MQ=255)
                                                                                                gAAAACTACTAGCACTTTTTTAAAAAATTTTTAAATTTAATAACTGAATTATTTTTCAAATTGTTAAATTAATTTCAAAACATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttttacaaa                                 <  1:1158987/128‑6 (MQ=255)
                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGATTTATTTGTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTACaaaa                              >  2:5345915/1‑128 (MQ=255)
                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCT‑‑TTTTTTTTTTTTTTTTTACaaa                               >  2:33465/1‑128 (MQ=255)
                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAaaaa                               >  1:2133576/1‑128 (MQ=255)
                                                                                                   aaataatataaattttttAAAAAATTTTTAATTTTAATTAATTAATTTTTTTTAAAAATTTTTAATTTTTTTAAAATAATTATATATTAATAAATTTTATTCTCG‑‑TTTTTTTTTTTTTTTTACaaaaa                             <  1:6763810/116‑1 (MQ=255)
                                                                                                    actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCT‑‑TTTTTTTTTTTTTTTTTTaaaaaa                             >  2:2537397/1‑128 (MQ=255)
                                                                                                     atattatcaattttttAAAAAATTTTTAATTTTAATAAATTTATTATTTTTCAAATTTTTAAATTATTTTAAAATAATTATATATTAATCAATTTTATGCCCGTTTTTTTTTTTTTTTTTTACaaaaa                             <  1:9623715/118‑1 (MQ=255)
                                                                                                        ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAATTCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAAtata                         >  2:4622215/1‑128 (MQ=255)
                                                                                                        ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTAaaaaaaaaataa                         >  1:2299330/1‑124 (MQ=255)
                                                                                                         taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTTTaaaaaaaaat                        >  2:9039535/1‑123 (MQ=255)
                                                                                                         taGCACTTTTTTAAAAAATTTTTAATTTAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATaa                        >  1:7854666/1‑128 (MQ=255)
                                                                                                          ttaatttttttAAAAAATTTTTTTATTTAATAAATTATTTATTTTTAAAATTTTTTAATTTTTTTAAAATAAATTTATATTAATAAATTTTTTTTTTTT‑‑TTTTTTTTTTTTTTTTTACAAAAATATaa                        <  2:1564247/123‑1 (MQ=255)
                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCT‑‑TTTTTTTTTTTTTTTTTACAAAAAaatatt                       >  1:4225078/1‑126 (MQ=255)
                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAat                       >  1:8366447/1‑128 (MQ=255)
                                                                                                             aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATAAAattta                    >  1:4041107/1‑128 (MQ=255)
                                                                                                             aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAAAATTattta                    >  2:6417413/1‑128 (MQ=255)
                                                                                                              cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCG‑‑TTATTTTTTTTTTTTTTAAAAAAATAAAatatat                   >  1:1609554/1‑128 (MQ=255)
                                                                                                               ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTTAAAAAAATATAattta                    >  2:7355292/1‑128 (MQ=255)
                                                                                                                         aaTTTTTAATTTTAATAAATTTATTATTTTTAAAATTTTTAAAATATTTTTAAATAATTATATATTAATAAATTTTATTATCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACg        <  2:1734240/128‑1 (MQ=255)
                                                                                                                              ||                                                                                                                                  
AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: