Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681634,9450GA33.3% 1438.3 / inf 1291intergenic (+49/+117)JCVSYN2_00105/JCVSYN2_00110ABC transporter permease/cell division protein FtsH
Reads supporting (aligned to +/- strand):  ref base G (727/47);  new base A (40/347);  total (819/472)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.06e-02

Only 100 of 1311 total aligned reads displayed.

CTTTACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTGTCT  >  CP016816/34818‑35071
                                                                                                                                      |                                                                                                                                       
  ttACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTTAAGTTGATGAATAATAAAAGATTAAAACAAATACATTTTATTTTGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aa                                                                                                                                   >  1:3111967/1‑128 (MQ=255)
       tcttTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTTGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaa                                                                                                                              >  2:3061656/1‑128 (MQ=255)
         tttATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGTTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaa                                                                                                                            >  1:204262/1‑128 (MQ=255)
              gAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAATATTAAACCAAATACATAGTGTTTTTTTTTATTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaaaaaaa                                                                                                                       >  1:1874051/1‑128 (MQ=255)
               aGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaaaaaaa‑‑a                                                                                                                    >  1:1048875/1‑128 (MQ=255)
                gTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAATTGAAGTTGATGAATAATAAAATATTAAACCAAATACATTGTTTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaaaaaaa‑‑aa                                                                                                                   >  2:869529/1‑127 (MQ=255)
                gTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTTTTAGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaaaaaaa‑‑aa                                                                                                                   >  2:3006994/1‑127 (MQ=255)
                 tttAAAAAAAAACAGTAATAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTTATTAATAAAAAAACATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑T‑‑‑‑aaaaaaaaaaaaaa‑‑aat                                                                                                                  <  1:3882715/128‑3 (MQ=255)
                  ttCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTAGATGAATAATAAAAGATTAAACCAAATACATTTTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaaaaaaa‑‑aata                                                                                                                 >  1:193074/1‑125 (MQ=255)
                    caacaaAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTCGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaaaaaaa‑‑aaaaat                                                                                                               >  2:932518/1‑126 (MQ=255)
                    caacaaAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTAAGTATAGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑T‑‑‑‑aaaaaaaaaaaaaa‑‑aaaaaa                                                                                                               >  2:1670732/1‑126 (MQ=255)
                    caacaaAAAGAGTAAGAAATGCAGAACCAAGTACAATGCTAAGAGAAGTTGATAAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTATTTATAT‑‑AATA‑‑TT‑‑‑G‑‑‑‑ATaaaaaaaaaaaa‑‑aaaaaa                                                                                                               >  2:3373457/1‑126 (MQ=255)
                     aacaaAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGAATAAACCAAATACATTGAGATTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaaaaaaa‑‑aaaaact                                                                                                              >  2:3424249/1‑125 (MQ=255)
                        aaaaaGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAAA‑‑aaaa‑‑‑aataaa                                                                                                        >  1:705426/1‑127 (MQ=255)
                          aaaGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTATGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑aaaaaaaaaaaaaa‑‑aaaaaaaaaaat                                                                                                         >  1:1577268/1‑120 (MQ=255)
                            agagTAAGAAATGTGGAAAAAAGTAAAATGCTATTGTATTTTTATGTATAATAAAATTTTAAACAAATTAATTTGTGTTTGTTTTTTTTATTT‑‑AATA‑‑TT‑‑‑T‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGc                                                                                                    <  2:2549783/128‑1 (MQ=255)
                              agTAAGAAATTCTGAACCAAGTACAATTCTAAGTGAAGTTGATGAATAATAAAATATTAAAACAAATACATTATGTTTTTTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑T‑‑‑‑aaaaaaaaaaaaaa‑‑aaaaaaaaaaattaaa                                                                                                     >  2:2260389/1‑116 (MQ=255)
                               gTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGTTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATaa                                                                                                >  1:2731148/1‑127 (MQ=255)
                               gTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGAGTTTGGTTTTTTTATAT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTgaa                                                                                                   >  1:1531826/1‑126 (MQ=255)
                                  aGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAAa‑‑aaa‑‑‑tataataatttgaataa                                                                                             >  2:3490218/1‑117 (MQ=255)
                                    aaaTGCTGAACAATTTAAAATTCTATGTTAAGTTGATGAATAATAAATCTTTAAACCTAATACATTTTGTTTGGTTTTTTTTTTT‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACta                                                                                              <  2:1272689/128‑1 (MQ=255)
                                      aTGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAATTTGAATAATATaa                                                                                           >  1:874576/1‑128 (MQ=255)
                                            aaCAAATTACAATGTTAAGTGAATTTTATTAAAAATAAAATATTAAAAAAAATAAATTTTGTTTGGTTTTTTTTTTT‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAAt                                                                                      <  1:3777295/128‑1 (MQ=255)
                                               cAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TAAAATATTCATAAAATAAAAAATtttt                                                                                  >  2:1339093/1‑128 (MQ=255)
                                               cAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATAGTGTTTGGTTTTTTTATTA‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATATTTTAATTAATATTAAAAAtttt                                                                                   >  1:1634154/1‑126 (MQ=255)
                                                aaGTACAATGCTAAGTGAAGTTGATGAATAATAAAACATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑AATAATTTAAATAATATaaaaaaatttt                                                                                  >  2:2381374/1‑123 (MQ=255)
                                                  ttaaaaTTCTAAGTTAAGTTTTTTAATAATAAAATATTAAACCAAATATATTTTGTTTGGTTTTTTTTTTT‑‑AATATTTT‑‑‑A‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGtttt                                                                                <  1:2965170/124‑1 (MQ=255)
                                                   tACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAAATTAAAAACTATAAAAAAATtttttta                                                                              >  2:1138363/1‑127 (MQ=255)
                                                   tACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCaa                                                                            >  1:2465384/1‑128 (MQ=255)
                                                    aCAATGCTAATTTAATTTGATTAATAATAAAATATTAAACCAAATAAATTTTTTTTGGTTTTTTTATTT‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTc                                                                              <  1:622249/128‑1 (MQ=255)
                                                      aaTGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑AAAAATTTAAATAAAATAAAAAATttttttaaaa                                                                            >  2:2033103/1‑124 (MQ=255)
                                                       aTGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑AAAAATTTTAATAATATAAAAAATTTTTTTTCaaa                                                                           >  1:1807967/1‑128 (MQ=255)
                                                          cTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAATTTAAATAAAATAAAAAATttttttaaaaaaaa                                                                        >  2:3674021/1‑120 (MQ=255)
                                                            aaGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑AAAAATTTTAATAATATAAAAAATTTTTTTTAAAATaatt                                                                      >  1:583434/1‑126 (MQ=255)
                                                            aaGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑AATAATTTACATAATATAAAAAAATTTTTTTCAAATaaatt                                                                     >  2:61916/1‑125 (MQ=255)
                                                             aGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAATTTTCAAAATATAAAAAATTGATTTAAAAATAATTa                                                                     >  1:1270410/1‑128 (MQ=255)
                                                                 aaGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑AAAAATTTGAAAAATTAAAAAAATTTTTTTTAAAATaaattataa                                                                 >  1:465387/1‑121 (MQ=255)
                                                                   gTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTTAATAAAATAAAAAATTATTATTAAAAAAAATaaaaaaa                                                               >  1:1324154/1‑122 (MQ=255)
                                                                    ttGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAAAAAAAAAAAAAAAAA‑‑AAAA‑‑‑TTTAATTTTAATAATAAAAAAAATTTTTTTTAAAAAAattattaata                                                               >  1:3059354/1‑124 (MQ=255)
                                                                     tgtttaataaaAAAATTTTAAACCAAAAAAATTTTGTTTTGTTTTTTTATTT‑‑TATA‑‑TTTAAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGa                                                              <  2:758823/123‑1 (MQ=255)
                                                                      gatgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑AATAATTTTAAAAATATAAAAAATTTTTTTTAAAAAAattattaaattat                                                            >  1:3358069/1‑121 (MQ=255)
                                                                      gatgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑AATAATTTAAAAAAAATAAAAAATTTTTTTAAAAAAAATTAtaaagaatt                                                            >  1:2668820/1‑120 (MQ=255)
                                                                        ttaataatAAAATATTAAAAAAAATACATTTTGTTGTGTTTTTTTATTT‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTaa                                                          <  2:1608962/126‑1 (MQ=255)
                                                                        tgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TTAAATTTGAATACTATAAAAAATTGTTTTAAAAATAAGAAtactgatgtaaa                                                         >  1:2421566/1‑117 (MQ=255)
                                                                             aataaAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAAATTTCATAAAAAAAAAAATTATTTATAAAATAAATATAAAAGAATAAAATTa                                                     >  1:1213804/1‑128 (MQ=255)
                                                                              ataaAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAT‑‑‑TTTAATTTGCATACTATAAAAAATTGTTTTTAAAATAAGTATTAAGTAGTAAATTTaa                                                    >  1:362347/1‑128 (MQ=255)
                                                                              ataaAAGATTAAACCAAATACATTGTGTTTGGTTTTTTAATTT‑‑AATA‑‑TTAAA‑‑‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑AAAAATTTAAAAAATAAAAAAAATTTTTTTAAAATTAAAATTAAAGattaaaattaaa                                                   >  2:2611698/1‑128 (MQ=255)
                                                                                  atttttAAACAAAATAATTTGTTTTTGGTTTTTTTATTT‑‑AATA‑‑TTTAAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTaaaaa                                                 <  2:3145139/124‑1 (MQ=255)
                                                                                    gATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAATTTAAAAAGAATAAAAAATTTTTTTTAAAATAAGTTTTAATTCTTAAATTTaaaaaaaa                                              >  2:2903362/1‑126 (MQ=255)
                                                                                      ttAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTAAAATAAGTATTCAGGAGTAAATTTAAAAAACCaa                                            >  1:3218056/1‑128 (MQ=255)
                                                                                      ttAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTAAAATAAGTATTCAGGATTAAATTTAAAAAACCAaga                                          >  1:1138366/1‑128 (MQ=255)
                                                                                      ttAAACAAAATAAATTTTTTTTTGTTTTTTTTTTT‑‑AATT‑‑TTTAAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCa                                             <  2:218025/128‑1 (MQ=255)
                                                                                      ttAAAAAAAATAAATTTGTTTTGTTTTTTTTATTT‑‑AATT‑‑TTTAAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCa                                             <  1:3536786/128‑1 (MQ=255)
                                                                                        aaaCCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATGA‑‑‑TATAATTTGAATAATTTAAAAAATTTTTTTTAAAATAAGTATTAAGTATTAAATTTAAAAAACAAagt                                          >  1:3720265/1‑127 (MQ=255)
                                                                                        aaaCAAAATAATTTGTTTTTGGTTTTTTTATTT‑‑AATATTT‑‑‑A‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAagat                                         <  1:1746903/128‑2 (MQ=255)
                                                                                         aaCCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑AAAAATTTGCATAATATAAAAAATTTTTTTTAAAAAAATTATTAAGGAGTAAATTTAAAAAACAAagat                                         >  2:399106/1‑127 (MQ=255)
                                                                                           ccAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAAATTTCATAATAAAAAAAATTTATTATCAATTAATAATTAAGTATTATATTTAAAAAAAAAagaata                                       >  2:3314409/1‑125 (MQ=255)
                                                                                            taatttatttGTGTTTTGTTTTTTTTTTT‑‑AATA‑‑TT‑‑‑T‑‑‑‑AAAAAAAAAAAAAAATATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTta                                      <  2:1020248/120‑1 (MQ=255)
                                                                                             aaataaatTTTTTTTTTTTTTTTTATTT‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGATTtat                                     <  1:950715/128‑1 (MQ=255)
                                                                                             aaaTACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAAAAAAAAAAAAAAAAA‑‑ATAA‑‑‑AATAATTTGAATAATAAAAAATATTTTTTTAAAAATAAGAATTAATTATTAAATTTaaaaaaaaaataatta                                      >  1:1455454/1‑118 (MQ=255)
                                                                                             aaaTACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATATTTTGCATAATATAAAAAATTTTATTTAAAATAATTATACATAATTAAATTTAAAAAAACAATATTtat                                     >  1:763256/1‑128 (MQ=255)
                                                                                             aaaTAATTTTTGTTTGGTTTTTTTATTT‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtat                                     <  2:3251704/128‑1 (MQ=255)
                                                                                              aaTACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAAA‑‑AAAAAATTATAATTTTAAAAATATAAAAATTTTTTTTAAAAAAAAATATTAAGAATAAAATTAAAAAAACCAAAATTtaa                                     >  1:2616440/1‑127 (MQ=255)
                                                                                               tttaaTTTTGTTTTGTTTTTTTTTTT‑‑AATT‑‑TT‑‑‑TAAAAAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                    <  2:283469/124‑1 (MQ=255)
                                                                                               acaCAATGTATTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATATTTTGAATAATATAAAAAATTTTTTTTAAAATAAGAATAAAGAATTAAATTTAAAAAACaaaattttaaaaa                                  >  2:455125/3‑118 (MQ=255)
                                                                                               aTACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTTAATAATATAAAAAATTTTTTTTAAAATAATTATTCAGTATTAAATTTAAAAAAAAAAGAGTTATaa                                   >  1:2364070/1‑128 (MQ=255)
                                                                                               aTACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAATTTGCATAATATAAAAAATATTTTTTAAAAAAATAATAAAGTATTAAATTTAAAAAAAAAAAAGAtatat                                   >  1:1688291/1‑127 (MQ=255)
                                                                                               aTACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAATTTGAATAAATTAAAAAATTTTTTTTAAAAAAATTATTAAGAATTAAATTTAAAAAACAAATATTTATaa                                   >  1:2317017/1‑128 (MQ=255)
                                                                                               aTACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGAATACTATAAAAAATTTTTTTTCAAATAATTATTAAGTATAAAATTTAAAAAAAAAATATTTATaaa                                  >  1:1371808/1‑128 (MQ=255)
                                                                                               aTAAATTTTTTTTTGTTTTTTTATTT‑‑AATT‑‑TTTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTTCATACTATAAAAAATTGTTTTTCAAATAAGTATTCATGAGTAAATTTAAAAAACCAAGAGTtata                                    <  1:3478911/128‑1 (MQ=255)
                                                                                                tACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTTCATAATATAAAAAATTGTTTTTCAAAAAATTATTAAGGAGTAAATTTAAAAAACAAATAGTTATaatt                                 >  2:819247/1‑126 (MQ=255)
                                                                                                aaaaTTGTTTTTTTTTTTTTTATTT‑‑ACAA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGATTTATaac                                  <  1:532231/125‑2 (MQ=255)
                                                                                                  aatttttttttGTTTTTTTATTT‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATc                                <  1:3674021/121‑1 (MQ=255)
                                                                                                   aTTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAAAAAAAAAAAAAAAAAA‑‑AAAA‑‑‑TAAAATTTGAATAATAAAAAAAATTTTTTTTCAAAAAATTATTAATTATTAAATTTAAAAAACAAAGATTAAAAAAt                                 >  2:3775510/1‑128 (MQ=255)
                                                                                                   aTTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAAAAAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTTAATAATATAAAAAATTTTTTTTAAAATAATTTTTAAGTATTAAATTTAAAAAAAAAAGAGTTATAAAta                                >  1:826793/1‑127 (MQ=255)
                                                                                                   aTTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAAAAAAAAAAAAAAAAA‑‑AAAA‑‑‑AAAAATTTCAAAACAATAAAAAATTTTTTTAAAAAAAATTAAACATTAGTAAATTTAAAAAAACAAGAGTaaaaaaaa                                >  2:2465465/1‑126 (MQ=255)
                                                                                                    ttGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTAAAATAAGTATTAATTATTAATTTTAAAAAAACAATATTTATAAATCtg                              >  1:1979480/1‑127 (MQ=255)
                                                                                                    ttGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATTATTTTGAAAATTTTAAAAATTTTTTTTTAAATAATTTTTAAGGTTTTAATTTAAAAAACAAAGAGTTATTAAtagtgg                            >  1:2527980/1‑123 (MQ=255)
                                                                                                    atgtaTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATTATTTTCAAAATAAAAAAAATTTTTTTTAAAATAAGTATAAAGTATTAAATTTAAATAACCAAGATTTAaaaaaaat                              >  1:2904394/2‑124 (MQ=255)
                                                                                                     tgtgTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTAAAATAATTATTCAGTATTAAATTTAAAAAACCAAAATTTATAAAACTTg                             >  2:3852251/1‑128 (MQ=255)
                                                                                                     tgtaTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TAAAATTTTAATAATATAAAAAATTTTTTTTAAAAAAATAATTAAGTATTAAATTTAAAAAAAAAAAAATTAAAAATATTg                             >  1:2268575/1‑128 (MQ=255)
                                                                                                      gtatttGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGAATAATATAAAAATTTTTTTTTAAAATATTTATTAAGATATTAATTTAAAAAAAAAAATTTTATAAATCtttg                            >  1:1001909/4‑126 (MQ=255)
                                                                                                        ttttGGTTTTTTTATTT‑‑ACTT‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGTAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGtt                          <  2:2087638/127‑1 (MQ=255)
                                                                                                        gtTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TAAAATTTGAATACTATAAAAAATAGTTTTAAAAAAAAGTATTCATAAGTAAATTTAAAAAACCAAGATTtatataaattagttt                         >  1:3820296/1‑117 (MQ=255)
                                                                                                          tttgTTTTTTTATTT‑‑ATTA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGtttt                        <  2:1458817/125‑1 (MQ=255)
                                                                                                          ttGTTTTTTTTATTA‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGtttt                        <  1:2091659/128‑1 (MQ=255)
                                                                                                          ttGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GA‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTAAAATAATTATTCAGGATTAAATTTAAAAAACAAATATTTATAAATATTGtttttt                       >  2:3196025/1‑128 (MQ=255)
                                                                                                           tGGTTTTTTTATTTTAAATT‑‑TA‑‑‑A‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGttttt                       <  1:2828004/128‑1 (MQ=255)
                                                                                                            ggTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑GAAAAAAAAAAAAAAAAAA‑‑ATAA‑‑‑AATAATTTGCATAATAAAAAAAATTTTTTTTAAAATAATAATTAAGTATTAAATTTAAAAAACAAATATTTATAAATATTGTtttt                        >  1:3175351/1‑128 (MQ=255)
                                                                                                             gTTTTTTTATTT‑‑ACTA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTg                     <  1:3021206/128‑1 (MQ=255)
                                                                                                              tttttttATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAATTATTCAGGAGTAAATTTAAAAAACCAAGATTTATAAATATTGGTTTTTTGt                    >  1:3308621/1‑128 (MQ=255)
                                                                                                              tttttttATTT‑‑ACTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGAATACTAAAAAAAATTTTTTTAAAAATAATTATTAATTATTAAATTTAAAAAACAAATATTTATAAATATTGGtttttttt                    >  2:1958516/1‑126 (MQ=255)
                                                                                                                tttttATTT‑‑AATA‑‑TTTAA‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGttt                  <  1:3576943/128‑1 (MQ=255)
                                                                                                                  tttattt‑‑aATA‑‑TT‑‑‑TAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTtat                <  1:2136575/128‑1 (MQ=255)
                                                                                                                       tt‑‑aCTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAATTAATTATTAAGGAGTAAATTTAAAAAACCAAGATTTATAAATCTTGGGTTTTTGTTTatatatt           >  2:2708593/1‑125 (MQ=255)
                                                                                                                       tt‑‑aCTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATAAAATAAAAAATTTTTTTTCAAATAATTATAAAGTATTAAATTTAAAAAACCAAAATTTATAAAAATTGTTTTTTTTtttattttat           >  2:3576943/1‑128 (MQ=255)
                                                                                                                       tt‑‑aCTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TATAATTTGAAAACTAAAAAAAATTTTTTTTCAAATAAGAATTAAGAAGTAAATTTAAAAAACCAAAAGTTATAAATATTGTTTTTTTTTTTAtattat           >  2:185754/1‑128 (MQ=255)
                                                                                                                       tt‑‑aCTA‑‑TT‑‑‑GAA‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑AATAATTTGCATACTATAAAAAATTTTTTTTAAAATAATTATTCAGGATTAAATTTAAAAAACAAAAAGTTAAAAATCTTGTTTTTTTTTTTAtattat           >  2:1748820/1‑128 (MQ=255)
                                                                                                                             tA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAAA‑‑AAAA‑‑‑TTTAATTTGGATACATTAAAAAATTTTTTTTAAAAAAAATATTAATGAATTAATTTAAAAAAACAAATGTTTTAAATAATGGTTTTTTTTTTTTTTTTTTAtttt     >  1:3407583/1‑127 (MQ=255)
                                                                                                                                  t‑‑‑G‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTGTCt  <  2:1191620/128‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CTTTACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTT‑‑ACTA‑‑TT‑‑‑G‑‑‑‑AAAAAAAAAAAAAA‑‑ATAA‑‑‑TATAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTGTCT  >  CP016816/34818‑35071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: