Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.74.5% 335.0 / inf 287intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (17/46);  new base . (73/111);  total (101/186)
Fisher's exact test for biased strand distribution p-value = 9.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

Only 100 of 316 total aligned reads displayed.

AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCTTTTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATAT  >  CP016816/202358‑202603
                                                                                                                                 |                                                                                                                          
  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGATTATCTTCACAAAA‑AAAAAAAAAACAATCCCAtta                                                                                                                          >  1:3122846/1‑127 (MQ=255)
   ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATAACCttaa                                                                                                                         >  2:3531241/1‑126 (MQ=255)
     attgtAAATAAAATAGTTAATTTTAATTAAAATAACATAAAATTAATAATTCTTATAATTTATAAACTCGTTATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCttaaaa                                                                                                                       <  1:102321/126‑5 (MQ=255)
     aGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCttaaaa                                                                                                                       >  2:3265749/1‑124 (MQ=255)
      gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCttaaaaa                                                                                                                      >  2:3419984/1‑123 (MQ=255)
      gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCACtaaaaaa                                                                                                                      >  1:3852786/1‑122 (MQ=255)
         tAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAATCAATCCACttaaaaatta                                                                                                                   >  2:1465842/1‑120 (MQ=255)
         tAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCttaaaaataa                                                                                                                   >  1:3141971/1‑120 (MQ=255)
           aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAAAAATCCCCtaaaaaattaaa                                                                                                                 >  2:2945680/1‑117 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑‑TAAAA‑AATtaaaaaaaa                                                                                                        >  1:3263757/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCC‑‑ATTAAAA‑ATTaaaaaaaa                                                                                                         >  1:3165260/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCTTCGTGATCTAATATTA‑TATTTGTATATCTTCACAAAT‑AAAAAAAAAACAAACCAC‑‑‑AAAAA‑AATaaaaaaaaa‑a                                                                                                      >  2:2223241/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTATT‑TATTTGTTTATCATCACAAAT‑AAAAAAAAAACAAACACATAaaaaaa‑aaaaaaaaaa                                                                                                          >  2:821697/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGCTATATTTTTT‑TATTATTTTATATTAAGAAAT‑AAAAATAAAACAATAC‑‑‑AATAAAa‑aataaaaaaaat‑aaaaa                                                                                                  >  2:2110148/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaa                                                                                                   >  2:376524/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTT‑TATTTTTTTATCTTCACAAAT‑AAAAAAAAAAAAAACACCTT‑‑‑AAA‑AATaaaaaaaaa‑aaaaaa                                                                                                 >  2:1061279/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAAACCC‑‑AAAAAAA‑AATaaaaaaaaa‑aaaaa                                                                                                  >  2:1547589/1‑128 (MQ=255)
                       tattttaaataaAATAACATAAAATTAAAAATTATTTAAATTTATATACACGTTTTATATTTTTT‑TATTTTTTTATATTAACAAAT‑AAAAAAAAAACAATACCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaa                                                                                                 <  1:3544420/124‑1 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCTTAAAAAT‑TATaaaaaaaaa‑aaaa                                                                                                   >  2:451919/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaa                                                                                                 >  2:3193363/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaa                                                                                                 >  2:3619315/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTATTA‑TATTTCTTTATCTTCACAAAT‑AAAAAAAAAACAATCACC‑‑‑TAAAA‑AATtaaaaaaaa‑aaaaaaa                                                                                                >  2:203155/1‑128 (MQ=255)
                        atgTTTATTAAAATAAAATAAAATTAATAATTTTTATAATTTTTTTACTTGTTTTTTATTTTTT‑TTTTTTTTTATTTTAAAAAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaa                                                                                                <  2:2997617/126‑1 (MQ=255)
                        aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAAAAAACCC‑‑‑ATAAAA‑AATaaaaaaaaa‑aaaaaaaa                                                                                               >  2:1952218/1‑128 (MQ=255)
                        aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATATTTT‑TATTTTTTTATAATCACAAAT‑AAAAAAAAAAAAA‑‑ACAAATTAAAA‑ATTaaaaaaaaa‑aaaaaaa                                                                                                >  2:2572302/1‑128 (MQ=255)
                         ttttaataatAATACAATAAAATTAATATATATAATAATTTTTATCATCGGTTTTTTTTTTTT‑TTTTTTTATATATTAAAAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAA‑AATaaaaaaaaa‑aaaaaaaa                                                                                               <  1:3250595/126‑1 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaa                                                                                               >  2:3179824/1‑128 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATACC‑‑‑ATAAAA‑AATaaaaaaaaa‑aaaaaaaaa                                                                                              >  2:3879196/1‑128 (MQ=255)
                          ttttattaaAAAAAAATAAAATTAAAAATTTTTTTAATTTTTATCCTTTTTTTATTTTTTTT‑TATTTTTTTATTTTAAAAAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaaa                                                                                              <  2:1158472/124‑1 (MQ=255)
                           ttaattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATACAC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaaaa                                                                                             >  1:2930303/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATATTTT‑TATTTGTATATCTTCAAAAAT‑AAAAAAAAAACAATCCAATTAaaaaa‑aaaaaaaaaaaa‑aaaaaaaaa                                                                                              >  2:2441219/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGAAGATCCTCGTGATATATTTTTT‑TATTTGTTTATCTTAACAAATAAAAAAAAAAACAAACCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaata                                                                                             >  2:3018495/1‑126 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCACATTAAAAAT‑aaaaaaaaaaaa‑aaaaaaaaaa                                                                                             >  1:982653/1‑128 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAAAAATCACC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaaaaaa                                                                                           >  1:3508557/1‑128 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGAAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATATTAACAAAA‑AAAAAAAAAAAAATACC‑‑ATAAAAA‑AATaaaaaaaaa‑aaaaaaaaaaaa                                                                                           >  2:749731/1‑128 (MQ=255)
                             aaataaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTTTTTATCTTCACAAAT‑AAAAAAAAAAAAAACAAC‑‑‑AAAAA‑AATaaaaaaaaa‑aaaaaaaaaaaaa                                                                                          >  2:128951/4‑127 (MQ=255)
                              attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTTATCTATTTTTT‑TATTTTTTTATCTTCACAAAT‑AAAAAAAAAACAA‑‑ACCAATAAAAA‑AATaaaaaaaaa‑aaaaaaaaaaaaa                                                                                          >  2:1761423/1‑127 (MQ=255)
                              attaaAATACCATAAACTTAAGAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAAACCCC‑‑TTAAAA‑TATaaaaaaaaa‑aaaaaaaaaaaaa                                                                                          >  2:891520/1‑127 (MQ=255)
                                 aaatTAACATAAACATAATATTTATTATAAAGTTTTACTTCTTGATCTATTTTTT‑TATTTTTTTATCTTAACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAA‑ATTTAAAAAAAA‑AAAAAAAAAAAACTaaa                                                                                      <  1:2223241/128‑1 (MQ=255)
                                  aaataaaaaaaaaTTAAAAATTCTTATAATTTACTTCCTAGTTATCTAATTTTT‑TAATTTTTTATATTAAAAAAA‑AAAAAAAAAAAAATCCCC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTaaa                                                                                      <  1:3375530/119‑1 (MQ=255)
                                   aaTACCATAAACTTAATAATTCTTATAATTTAGATCCTCGTGATCTATTTTAT‑TATTTTTTTATCTTAACAAAT‑AAAAAAAAAAAAAAACAC‑‑TTAAAA‑ATTAaaaaaaaa‑aaaaaaaaaaaaaaaaaa                                                                                     >  2:663052/1‑128 (MQ=255)
                                   aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTAaaaaaaaa‑aaaaaaaaaaaaaaaaaa                                                                                     >  2:3444236/1‑128 (MQ=255)
                                    aaaataTAAAATTAATAATTTTTATAATTTATTTACTGTTTTTTTATTTTTT‑TATTTTTTTATATTAAAAAAA‑AAAAAAAAAAAAAACCCC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTaaaaa                                                                                    <  1:3080428/123‑1 (MQ=255)
                                        cATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAAAAATCCC‑‑ATTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAAATAaaaaaaaa                                                                                >  2:230255/1‑128 (MQ=255)
                                        cATAAAATTAATAATTATTATAATGTTTATACTCGTTATATATTTTTT‑TATTTTTTTATATTAAAAAAA‑AAAAAAAAAAAAATCCCC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAaaacaaa                                                                                <  1:3187775/128‑1 (MQ=255)
                                           aaaaataaaaaTTATTATAATTTATATACACTTTATTTATTTTTT‑TATTTTTTTATATTCACAAAA‑AAAAAAAAAAAAAACCCCTT‑‑‑AAA‑AAT‑AAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTa                                                                          <  1:1276140/123‑1 (MQ=255)
                                              attAATAATTTAAATAATTTATAAACTCTGGATAAATTTTTT‑TATTTTTTTATATTAAAAAAA‑AAAAAAAAAAAAATACCCTTAAAAAT‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑ctct                                                                           <  1:3743939/127‑1 (MQ=255)
                                                  ataatTCTTATAATGTAGAAACTAGTTATATATTTTTT‑TATTTTTTTATCTTAACAAAT‑AAAAAAAAAAAAATACCCTT‑‑‑AAA‑AAT‑TAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTa                                                                   <  1:1378216/128‑1 (MQ=255)
                                                     aatCTTATAATGTAGATCCTCGTGATCTATTATTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAA‑‑ACCAAAAAAAA‑ATTAAAAAAAAATAAAAAAAAAAAAAAAAAAACAAA‑CTAAAtttataa                                                                   >  2:919304/3‑124 (MQ=255)
                                                       ttataaaaTTTATATACTAGTTATCTATTTTTT‑TAATTTTTTAACTTAAAAAAA‑AAAAAAAAAAAAAACCCC‑TTAAAAA‑TTAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTaaa                                                                 <  1:1071951/122‑1 (MQ=255)
                                                                  aGATCCTAGTTTTATATTTTTT‑TTTTTTTTTTTATTAAAAAAA‑AAAAAAAAAAAAAACCCC‑‑TTAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑Ctattat                                                    <  1:3619315/128‑1 (MQ=255)
                                                                     tctacttgtgatatttttt‑tattttttTATATTAAAAAAA‑AAAAAAAAAAAAATACCC‑‑TTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATAcc                                                 <  1:892477/117‑1 (MQ=255)
                                                                     tacTCGTTATCTATTTTTT‑TATATGTTTATATTAACAAAT‑AAAAAAAAAACAATACCCTT‑‑‑AAA‑AAT‑TAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACcac                                               <  1:579526/126‑1 (MQ=255)
                                                                      aaatatttattATTTTTT‑TAATTTTTTAAAAAAAAAAAA‑AAAAAAAAAAAAATACC‑‑ATAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACca                                                <  1:1590884/118‑1 (MQ=255)
                                                                       tattgtttttttttttt‑ttttttttttttttAAAAAAA‑AAAAAAAAAAAAAAACCCTTT‑‑AAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACcac                                               <  2:3368480/117‑1 (MQ=255)
                                                                        ttgttttatttttttt‑ttttttttTATTTTAAAAAAA‑AAAAAAAAAAAAATAACATTAAAAAATAAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATAcc                                                 <  1:212113/118‑1 (MQ=255)
                                                                         tttatatattttttt‑tATTT‑TTTATATTAACAAAA‑AAAAAAAAAAAAATCACCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACattt                                           <  1:3572385/119‑1 (MQ=255)
                                                                           ttaTCTATTTTTTATATTT‑TTTATTTAAAAAAAA‑AAAAAAAAAAAAAACACC‑‑ATAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACattt                                           <  1:866064/126‑1 (MQ=255)
                                                                            tatatatttttt‑tattttttTATTATTAAAAAT‑AAAAAAAAAACAATCCCC‑TTAATAA‑TTAAAAAAAAAA‑AAAAAAAAAAAACTAAACACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACattt                                           <  2:371498/124‑1 (MQ=255)
                                                                            tatatatttttt‑tattttttTATCATTAAAAAT‑AAAAAAAAAACAATTCCC‑‑TTAAAA‑TTTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACatttt                                          <  1:3442654/124‑1 (MQ=255)
                                                                              tatttttttt‑ttttTGTTTTTATTCACAAAT‑AAAAAAAAAAAAATACCC‑TTAAAAA‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACatttta                                         <  1:2970623/124‑1 (MQ=255)
                                                                              tatATTTTTT‑TTTTTTTTTATATTAAAAAAT‑AAAAAAAAAAAAAAACAC‑TTAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACatttta                                         <  1:2956551/126‑1 (MQ=255)
                                                                              aaaaTTTTTT‑TATTTGTATATATTAAAAAAA‑AAAAAAAAAACAAAACCCTTAAAAAT‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACatttt                                          <  1:1526262/125‑1 (MQ=255)
                                                                                tttttttt‑tatttttttaTATTAAAAAAT‑AAAAAAAAAAAAATAACCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACattttatttt                                     <  2:3395194/126‑1 (MQ=255)
                                                                                 aTTTTTT‑TATTTGTTTAAATTAAAAAAA‑AAAAAAAAAAAAATACCC‑‑TTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACattttatttt                                     <  1:3688227/128‑1 (MQ=255)
                                                                                  tttttt‑tatttttttaTCTTAACAAAT‑AAAAAAAAAACAATCCCCAT‑‑‑AAA‑AAT‑AAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTaa                                  <  1:3228072/128‑1 (MQ=255)
                                                                                  tttttt‑tATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAACAAAAAACAAACCACAAATAATTAAAATACC‑CTTTTATACCCAATTATAttttt                                    >  2:278313/1‑128 (MQ=255)
                                                                                  tttttt‑tAATTTTTTATCTTCACAAAT‑AAAAAAAAAACAATCCC‑‑ATTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTAttttt                                    <  1:2866647/128‑1 (MQ=255)
                                                                                  attttt‑tATTTGTTTATATTCAAAAAT‑AAAAAAAAAA‑AATCACC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTa                                   <  1:1196883/127‑1 (MQ=255)
                                                                                  attttt‑tATTTGTTTATATACAAAAAA‑AAAAAAAAAAAAAACCCC‑‑‑TAAAA‑AATTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTa                                   <  2:3641617/127‑1 (MQ=255)
                                                                                   ttttt‑tattt‑tttaTATTAAAAAAA‑AAAAAAAAAAAAAAACCC‑‑TTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTaa                                  <  1:3090145/128‑1 (MQ=255)
                                                                                    tttt‑tatttttttattTTAAAAAAA‑AAAAAAAAAATAA‑ACCCATTTAAAA‑TTAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTa                                   <  1:2403657/128‑1 (MQ=255)
                                                                                    tttt‑tATTTTTTTATCTTAAAAAATAAAAAAAAAAACAATCCCCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAGAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTaa                                  <  1:2299585/128‑1 (MQ=255)
                                                                                    tttt‑tATTT‑TTTTTATTAAAAAAA‑AAAAAAAAAAAAAAACCC‑TTAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTaa                                  <  1:476096/128‑1 (MQ=255)
                                                                                    atattatatttattATATTAAAAAAA‑AAAAAAAAAATAAACCCC‑‑TAAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTAATTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTa                                   <  1:2634638/116‑1 (MQ=255)
                                                                                     ttt‑tATTTTTTTATCTTAAAAAAA‑AAAAAAAAAAAAAAACCC‑TAAAAAA‑TTAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTaa                                  <  1:2160335/128‑1 (MQ=255)
                                                                                      tt‑tATTTTTTTATAATAAAAAAT‑AAAAAAAAAA‑AAT‑‑ACTATT‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATa                              <  1:1818579/128‑1 (MQ=255)
                                                                                       t‑tATTTTTTTATCTTAACAAAA‑AAAAAAAAAAAAATACCC‑‑TTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAAt                               <  1:3534162/128‑1 (MQ=255)
                                                                                       t‑tATTTTTTTATATTTAAAAAA‑AAAAAAAAAAAAAAACCC‑ATAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTaaaa                                <  1:1329813/128‑1 (MQ=255)
                                                                                          tttttttTAT‑TTCAAAAAT‑AAAAAAAAAAAAATACCATTAAAAAT‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATa                              <  1:2090225/127‑1 (MQ=255)
                                                                                           ttttttTAT‑TTAACAAAA‑AAAAAAAAAAAAAACACCATAAAAAT‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATaa                             <  1:775029/128‑1 (MQ=255)
                                                                                              ttttttattaataaaa‑aaaaaaaaaaaaATACCC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGata                         <  1:2014271/116‑1 (MQ=255)
                                                                                              tttaatattaaaaaaa‑aaaaaaaaaaCAATACC‑‑ATTAAAA‑ATTTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGata                         <  1:190490/116‑1 (MQ=255)
                                                                                               ttttatttCAAAAAA‑AAAAAAAAAAAAAAACCC‑TTAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGata                         <  2:3123633/122‑1 (MQ=255)
                                                                                               tttaatatataaaaT‑AAAAAAAAAA‑AAAAACCATTAAAAA‑TTAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGata                         <  2:3330518/117‑1 (MQ=255)
                                                                                               tttATCTTCACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAA‑‑AAAACCAAA‑ATATATTTATTAAAATTAC‑ATATTAAACCAAATATAATTTTTAAAAAAAGATAttta                     >  2:3150396/1‑126 (MQ=255)
                                                                                                ttaatataaaaaaT‑AAAAAAAAAAAAATAACC‑‑ATAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATAtt                       <  1:3826262/118‑1 (MQ=255)
                                                                                                   ttaattaaaaa‑aaaaaaaaaaaaaaACCC‑‑TTAAAA‑AAT‑AAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  1:1350260/121‑1 (MQ=255)
                                                                                                   tcataaaaaaT‑AAAAAAAAAAAAATCCCC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGAc                    <  2:3072440/121‑1 (MQ=255)
                                                                                                    tataaaaaataaaaaaaaaaaaaATCCCC‑‑‑TAAAA‑AATTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  2:3793067/117‑1 (MQ=255)
                                                                                                    attaacaAAT‑AAAAAAAAAAAAAAAACC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  1:3331400/124‑1 (MQ=255)
                                                                                                     ttaaaaaaa‑aaaaaaaaaaaaATAACC‑ATAAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  2:3552557/123‑1 (MQ=255)
                                                                                                     taaaaaaaa‑aaaaaaaaaaaaaaACCC‑‑TTAAAA‑AAT‑AAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                 <  1:1989921/123‑1 (MQ=255)
                                                                                                     aataaaaaa‑aaaaaaaaaaaaaaaaCCATAT‑AAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  1:2751028/123‑1 (MQ=255)
                                                                                                       aaaaaaT‑AAAAAAAAAACAATACCC‑‑TTAAAA‑AAT‑AAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACataat               <  1:1136955/125‑1 (MQ=255)
                                                                                                        acaAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAACAAAAACCAAA‑AAAAATTTATAAAAATACCAATATT‑TACCACTTTTTTTTTTTAAAAAAATAtttttaataaaaa              >  2:292827/1‑119 (MQ=255)
                                                                                                         aaaaa‑aaaaaaaaaaCAAAACC‑‑ATTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata              <  1:519753/127‑1 (MQ=255)
                                                                                                                  aaaaaaaCAATAACCTTAAAAAT‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatatatt        <  1:698782/128‑2 (MQ=255)
                                                                                                                     aaaaaaaaacccATTAAAAA‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTAC‑CTATTATACCAAATTTTATTTTTAAAATAAGATATTGACATAATATATATCTaa     <  1:2521671/119‑1 (MQ=255)
                                                                                                                     aaaaCAATCCCC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAA‑AAAAAACAAA‑AACTATTTATTAAAATTAC‑CTTTTTTAAAACATTTTATTTTTAAAATAAAATATTCACATAatatataaataaaaa  >  1:3049103/1‑120 (MQ=255)
                                                                                                                                 |                                                                                                                          
AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTT‑TATTTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCTTTTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAA‑CTCTATTTCTTAAAATTCC‑CTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATAT  >  CP016816/202358‑202603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑