Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 242,857 G→A G829D (GGT→GAT)  JCVSYN2_01035 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816242,8570GA99.7% 5477.6 / NA 1721G829D (GGT→GAT) JCVSYN2_01035ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (810/904);  minor base T (0/5);  total (811/910)
Fisher's exact test for biased strand distribution p-value = 6.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1721 total aligned reads displayed.

AAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242732‑242981
                                                                                                                             |                                                                                                                            
aaGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGAtt                                                                                                                            <  1:3886672/128‑1 (MQ=255)
aaGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGAtt                                                                                                                            <  1:996252/128‑1 (MQ=255)
  gAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTa                                                                                                                          >  1:255962/1‑128 (MQ=255)
    aCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGc                                                                                                                        >  2:78820/1‑128 (MQ=255)
    aCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGc                                                                                                                        <  1:50504/128‑1 (MQ=255)
    aCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTAAGCTTCTTCTCGTAAAAAAAATATCTTTTTATAAGTAACTCTTGTTGGAATTTCACCTATGTTTATTTCAGCAGAttatgc                                                                                                                        >  2:1702285/1‑124 (MQ=255)
      ttGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCaa                                                                                                                      <  2:407383/128‑1 (MQ=255)
      ttGATTTAAAAAAGCTAAGTGTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCaa                                                                                                                      <  2:3397779/128‑1 (MQ=255)
        gATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    >  1:1220485/1‑128 (MQ=255)
        gATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    <  2:1882118/128‑1 (MQ=255)
         aTTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTc                                                                                                                   <  2:1758393/128‑1 (MQ=255)
          tttcataaaGCTAAGTTTACTACTAAATTTAGTGTAACTTTAGCTTCTTCTGGTAACAAAAATATCTTTTTATTCCTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGAAATTcc                                                                                                                  <  2:1125774/122‑1 (MQ=255)
          tttAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTcc                                                                                                                  >  1:637235/1‑128 (MQ=255)
           ttAAAAAAGCTAAGTATACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCa                                                                                                                 <  2:1971492/128‑1 (MQ=255)
           ttAAAAAACCTTAGATTAATACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCa                                                                                                                 <  1:1658953/128‑1 (MQ=255)
             aaaaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGATTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCAATc                                                                                                               >  1:1154234/1‑128 (MQ=255)
              aaaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCa                                                                                                              >  1:446318/1‑128 (MQ=255)
                 aaccTAAGTTTACTACTTAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAttgatggaatt                                                                                                           <  1:3402180/125‑10 (MQ=255)
                 aaGCTAAGTTTACTACTAAATTTATTTTAACATTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTCACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAAtt                                                                                                           <  2:3508885/128‑1 (MQ=255)
                  aGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTg                                                                                                          <  2:2944797/128‑1 (MQ=255)
                   gCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAATATATCTTTTTATTAGAAACTGTTGTTTGAATTTCAACTTTGTTTATTTCAGCAGAATTAGCAATTCAATCAAttac                                                                                                         >  2:1346211/1‑126 (MQ=255)
                    cTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCt                                                                                                        <  1:1966480/128‑1 (MQ=255)
                    cTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCt                                                                                                        >  2:1283657/1‑128 (MQ=255)
                    cTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTATTATTAGTAACTGTTGTTGGAATTTCAACTATGATTATTTCAGCAGATTTAGCAAATACATCAAATGCt                                                                                                        >  1:3606844/1‑128 (MQ=255)
                     tAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAAGTATGTTTATTTCTGAAGATTTATCAATTCAATCATTTGCtt                                                                                                       >  1:161167/1‑128 (MQ=255)
                     tAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATTTTTATTTCAGCAGATTTATCAATTCCATCAATTGCta                                                                                                       >  1:3008883/1‑127 (MQ=255)
                     tAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtt                                                                                                       <  1:1285651/128‑1 (MQ=255)
                     tAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtt                                                                                                       >  2:1064550/1‑128 (MQ=255)
                      aactttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCttt                                                                                                      <  2:925843/125‑1 (MQ=255)
                       aGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtttt                                                                                                     >  1:2173525/1‑128 (MQ=255)
                       aGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtttt                                                                                                     >  1:1330782/1‑128 (MQ=255)
                        gTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTa                                                                                                    >  2:2629859/1‑128 (MQ=255)
                        gTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTa                                                                                                    >  2:485665/1‑128 (MQ=255)
                        gTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTAAACTATGTTTATTAAAGCAGATTTAGCTATGCCATCAAtttatatta                                                                                                    >  2:2467668/1‑121 (MQ=255)
                         tttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGTTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAc                                                                                                   >  1:3486549/1‑128 (MQ=255)
                         tttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAc                                                                                                   >  2:555649/1‑128 (MQ=255)
                          ttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACt                                                                                                  <  2:2317596/128‑1 (MQ=255)
                            actaatAAATTTAGTTTAACTTTAGCTTCTTCTGTTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTTGAATTTCAAATATGTTTATTTCATCATATTTAGCAATTCCATCAATTGCTTTTACTAt                                                                                                <  1:907787/128‑1 (MQ=255)
                                  aaaTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaata                                                                                          >  2:2748627/1‑126 (MQ=255)
                                  aaaTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaaa                                                                                          <  1:3544498/128‑1 (MQ=255)
                                   aaTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTAATTTCAGCAGATTTAGCAATTCCATCAATTTATTTTACTATTAAAAac                                                                                         >  2:2547989/1‑128 (MQ=255)
                                    aTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTATCAATTCCATCAATTGCTTATACTATTAAAAaca                                                                                        >  1:1560623/1‑128 (MQ=255)
                                      ttAGTTTAACTTTAGCTTCTTCTGTTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACt                                                                                      <  2:3124052/128‑1 (MQ=255)
                                        cttttAAGTTATGCTTCTT‑TGGTTAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttat                                                                                   <  2:201041/126‑1 (MQ=255)
                                        aGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACtta                                                                                    >  1:1713801/1‑128 (MQ=255)
                                          tatAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTAATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttatt                                                                                  <  2:2058692/126‑1 (MQ=255)
                                             aaCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATtata                                                                               <  2:2809365/128‑1 (MQ=255)
                                              aCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGATTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaa                                                                              >  1:2949802/1‑128 (MQ=255)
                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATTTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             >  2:2238403/1‑128 (MQ=255)
                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             <  2:3596255/128‑1 (MQ=255)
                                                tttAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaaa                                                                            <  2:3692947/128‑1 (MQ=255)
                                                 tatgattttattGGTAAAAAAAATAT‑ATTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGt                                                                          <  2:1819979/117‑1 (MQ=255)
                                                   aGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTa                                                                         >  1:1001673/1‑128 (MQ=255)
                                                    gCTTCTTCTGGT‑AAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCTACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTAtt                                                                       <  1:826182/128‑1 (MQ=255)
                                                     cttcttCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTAtt                                                                       <  2:3768854/128‑1 (MQ=255)
                                                      ttcttcTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTa                                                                      <  1:3130595/128‑1 (MQ=255)
                                                      tgcttctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTa                                                                      <  2:3336247/126‑1 (MQ=255)
                                                        cttctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTaaa                                                                    >  1:3328594/1‑128 (MQ=255)
                                                        cttctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTaaa                                                                    >  1:1406418/1‑128 (MQ=255)
                                                        cttctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTaaa                                                                    >  2:64592/1‑128 (MQ=255)
                                                          tctGGTAAAATAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAata                                                                  <  2:159194/128‑1 (MQ=255)
                                                               tAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCCTCAATTGCTTTTACTATTACAAACACTTATTATAAAAGTATTAAATATGCac                                                             >  2:859080/1‑127 (MQ=255)
                                                               tAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCaa                                                             >  2:1438513/1‑128 (MQ=255)
                                                                   aaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATTCAAATGa                                                         >  1:2750913/1‑128 (MQ=255)
                                                                   aaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGa                                                         >  1:3288808/1‑128 (MQ=255)
                                                                     taaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCAATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAat                                                       <  1:1489895/127‑1 (MQ=255)
                                                                     taaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTATATTTCAGCAGATTTAGCAATTACATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAat                                                       <  1:1849192/127‑1 (MQ=255)
                                                                      aaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAata                                                      <  2:1206381/128‑1 (MQ=255)
                                                                        taccttataattaGTAACTGTTGTTGGAATTGGAACTATGTTTATTAGTGCTGATTTATCTATTCCATCAATTTCTTTTACTATTAAATACACTTATTATAAATGTATTAAATATGCAATTGAAtata                                                    <  2:736293/119‑1 (MQ=255)
                                                                         atCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATaa                                                   >  1:77201/1‑128 (MQ=255)
                                                                            tttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGAAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAatta                                                <  1:2878424/128‑1 (MQ=255)
                                                                            tttttATTAGTAACTGATGTTGGAATTTCAACTATCTTTATTTCAGCAGATTAAGCAATTACATCAAGTACATTAACTATTAAAAACACTTATAATAAAAGAATTAAATAAGCAAAACAATAAAatta                                                >  1:2485164/1‑128 (MQ=255)
                                                                             ttttATTTGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAattat                                               <  2:3287123/128‑1 (MQ=255)
                                                                             ttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAATCACTTATTATAAAAGTATTAAATATGCAAATGAATATAattat                                               >  1:378550/1‑128 (MQ=255)
                                                                             ttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAattat                                               >  2:3190325/1‑128 (MQ=255)
                                                                               ttattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAATATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTc                                             >  1:191390/1‑128 (MQ=255)
                                                                                tattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCa                                            >  2:2393177/1‑128 (MQ=255)
                                                                                        aCTGTTGTTTGAATTTCAACTATGTTTATTTAATCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGgtgt                                    <  1:2016465/128‑1 (MQ=255)
                                                                                        aCTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGgtgt                                    >  2:2875172/1‑128 (MQ=255)
                                                                                         gttttgttgGAAGTTCAACTATGTTTATTTCAGCAGAATTAGCAATTCCATCAATTTCTTTTACTATTAAAAACACTTATTATAAAAGTCTTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   <  2:2154004/125‑1 (MQ=255)
                                                                                         cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   >  1:1780598/1‑128 (MQ=255)
                                                                                         cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   <  1:2368346/128‑1 (MQ=255)
                                                                                          tgttgtTTGAATTTCAAATATGTTTATTTCAGAAGATTTAGCAATTCCATCAATTGATTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGttt                                  <  1:564249/128‑1 (MQ=255)
                                                                                          tgttgtTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGttt                                  >  1:1083282/1‑128 (MQ=255)
                                                                                           gttgttGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 <  1:3709836/128‑1 (MQ=255)
                                                                                            ttgttgGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCa                                <  1:2108530/128‑1 (MQ=255)
                                                                                                 tgAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtc                           <  2:689540/127‑1 (MQ=255)
                                                                                                        cAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGTTGTTTCAAATTCTCCtttt                    >  2:1626609/1‑127 (MQ=255)
                                                                                                         aaCTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTaa                   >  2:3050581/1‑128 (MQ=255)
                                                                                                         aaCTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTaa                   <  1:2260806/128‑1 (MQ=255)
                                                                                                          aCTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAAc                  >  1:242531/1‑128 (MQ=255)
                                                                                                           cTATGTTTATTTCAGCAGATTTAGCTATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACa                 <  1:3838453/128‑1 (MQ=255)
                                                                                                           cTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACa                 <  1:2788119/128‑1 (MQ=255)
                                                                                                               gTTTATTTCAGCAGATTTAGCAATTACATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTAATAAATATGCAAATGAATATAATTATAAAAAAGTATTTACAAATTCTCCTTAAACAAAAc             >  1:308516/1‑128 (MQ=255)
                                                                                                                tttATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAcc            <  2:3464116/128‑1 (MQ=255)
                                                                                                                tttATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGAGTTTCAAATTCTCCTTTAACAAAAcc            >  1:3214076/1‑128 (MQ=255)
                                                                                                                  tATTTCATCAGATTTAGCAATTCCATCTATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGAAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCaa          <  2:1515705/128‑1 (MQ=255)
                                                                                                                     ataagcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGa       <  1:2641563/125‑1 (MQ=255)
                                                                                                                        atcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatta    <  1:695337/126‑1 (MQ=255)
                                                                                                                          cagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGattaat  >  2:3840436/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
AAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242732‑242981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑