Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2520T.59.2% 8.3 / inf 1904intergenic (+216/‑326)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (356/314);  new base . (721/251);  total (1090/814)
Fisher's exact test for biased strand distribution p-value = 1.78e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2419 total aligned reads displayed.

AATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTT  >  CP016816/165125‑165379
                                                                                                                                 |                                                                                                                                  
aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                                                    >  1:2704558/1‑128 (MQ=255)
    tttATTTTCATTAATTTATCGCACTTGTATTTATTATTAATAAACTATAAATATTATATTTTTTTATATTTTTTTTATA‑TGCAAT‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAAAAGCttttttt                                                                                                                                <  1:5240040/128‑1 (MQ=255)
      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                                                              >  2:5951742/1‑128 (MQ=255)
      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                                                              >  2:498074/1‑128 (MQ=255)
        ttttCATTAATTTTTACCACTTTTATTAATTAGTAATAAAAAAAAAATATATTATTTTTATAATTTTTTTTTATA‑TTCAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGCttttttttttt                                                                                                                            <  1:3509113/127‑1 (MQ=255)
            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                                                        >  1:21863/1‑128 (MQ=255)
            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttatttttttt                                                                                                                        >  1:6485890/1‑128 (MQ=255)
               tAATTTTTTGCACTTGTATTTATTATTAATAAACTAAAAATATTATATTTTTAGATATTTTTTTTTTA‑TTGAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCgttttttttttttttttt                                                                                                                     <  2:6665468/128‑1 (MQ=255)
                  tttATCGCACTTGTAAGAATTATTAATAAAAAAAAAATATTATCTTTTTATATTTTTTTTGTATA‑TTCAAA‑ATTTTAAAAAATTAATTTTAACTTGTAAAAAAAAGCttttttttttttttttataat                                                                                                                  <  1:6857263/128‑6 (MQ=255)
                    tATTGCACTTTTATTTAATATTAATAAAATAAAAATATTATATTTTTAGTTATTTTTTTTTTA‑TTCAAA‑TTTTTAAAAAAATAATTTTAACTTTTAAAAAATACCttttttttttttttataatggaa                                                                                                                <  2:5878607/128‑10 (MQ=255)
                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGTTTTTTTTTTTTTTTTTTTaatgtaaa                                                                                                             >  1:4675146/1‑123 (MQ=255)
                          aatttttttaaTTAATAAGTAAATATAACTTTTTTTTTTTTAATAATTTTTTTTTAA‑TTTAAA‑ATTTTTAAAAATTAATTTTAAATTTTAAAAAAAA‑‑‑‑TGTTTTTTTTTTTTTTTATAATGGAAAtata                                                                                                      <  2:4728421/119‑1 (MQ=255)
                           attGTATGAATTATTAAGAAAATAAAAAATTTATTTTTATATATATTTTTTGTATA‑TTCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAAtata                                                                                                      <  2:4010351/127‑1 (MQ=255)
                            ttttATTAAATATTAATAAAATAAAAATATTATATTTTTATTTATTTTTTTTTTA‑TTCAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                                                     <  1:2562431/125‑1 (MQ=255)
                            ttttATGAATTAGTAATAAACTAAAAATAATATCTTTTTAGATATTTTTTGTATA‑TTCAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                                    <  2:2946067/125‑1 (MQ=255)
                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTataatataaatataa                                                                                                        >  2:1457808/1‑119 (MQ=255)
                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAAtata                                                                                                      >  2:3297540/1‑128 (MQ=255)
                            atttATTAATTTTTAATAAAATAAAAAAATTATCTTTTTATATTTTTTTTTTATATTGAAAA‑TTTTAAAAAAATAAATTT‑AACTTTTAAAAAAAACC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                                    <  2:4812586/125‑1 (MQ=255)
                              gTATGAATTATTAAGTAACAAAAAATATTATCTTTTTATTTATTTTTTGTTTA‑TTCAAA‑TTTTTAAAAAATTAATTTGAAATTTTTAAAAATA‑‑‑‑TATTTTTTTTTTTTTTTATAATGGAAATATAAA‑tt                                                                                                 <  1:156092/128‑1 (MQ=255)
                              gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTA‑AATTGAAATATaa                                                                                                     >  2:6261053/1‑128 (MQ=255)
                              gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTATTatatgtaaaaataaaa                                                                                                      >  2:1970340/1‑116 (MQ=255)
                               tttttaatattaataaAATAAAAATATTATATTTTTATATATTTTTTTTTTA‑TTCAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑tt                                                                                                 <  1:5949649/118‑1 (MQ=255)
                               tATGTATTTTTAATAAACTAAAACTATTATATTTTTTTATATTTTTTTTATA‑TGCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑tt                                                                                                 <  2:768640/128‑1 (MQ=255)
                               tATGTATTAGTAATAAAATAAAAATATTATCTTTTTTTATATTTTTTTTATA‑TGCAAA‑TTTTTAAAAAAATAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑tt                                                                                                 <  1:4780168/128‑1 (MQ=255)
                               tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAA‑t                                                                                                  >  2:209138/1‑128 (MQ=255)
                                tttaaTTATTAATAAAAAAAAATTATTATTTTTTTTGATATTTTTTTTATA‑ATGAAA‑TTTTTAAAAAATTTATTTTAAATTTTTAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑tt                                                                                                 <  2:1331546/125‑1 (MQ=255)
                                  gatttAGTTATAAATTTAAATTTTTATTTTTTTTGTTATTTTTTTTTTA‑TTAAAA‑TTTTTAAAAAATTTATTTGTAATTTTTAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑tttt                                                                                               <  1:3682189/125‑1 (MQ=255)
                                    attattTAGAAAATAAAACTTTTATATTTTTTTATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTTATTTGAAAATGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGt                                                                                           <  1:3811627/128‑1 (MQ=255)
                                     ttattaATAAAATAAAAATATTATATTTTTATATATTTTTTTTATT‑TTCAAATTTTTAAAAAATTTAATTTTAA‑TTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGt                                                                                           <  1:186057/128‑1 (MQ=255)
                                     ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTAAAATATAAA‑TTTTattt                                                                                           >  1:1463461/1‑125 (MQ=255)
                                      tattaataaACTAAAAAAATAATATTTTTATATATTTTTTGTTTA‑TTCAAA‑TTTTTAAAAAATTAAATTTAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTa                                                                                          <  2:6159428/125‑1 (MQ=255)
                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATATTGGAAATATAAA‑TTTTatttat                                                                                         >  1:7243012/1‑123 (MQ=255)
                                       attaataaAATATAAATATTATGTTTTAATTTATTTTTTTTTTA‑TTTATA‑TTTTTAAATATTTATTTTAAAATTTTAAAAATTTGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAA‑TTTTAGGTAt                                                                                         <  1:5080174/126‑1 (MQ=255)
                                        ttaataaAATAAAACTATTATTTTTTTTTTTATTTTTTTTATA‑TTCAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTAt                                                                                         <  1:672754/127‑1 (MQ=255)
                                        gtaattaaataaaaataTTATATTTTTATATTATTTTTGTATA‑TTCAAA‑ATTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTAttt                                                                                       <  1:5523913/119‑1 (MQ=255)
                                        gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAA‑TTTTattaatt                                                                                        >  1:3371596/1‑122 (MQ=255)
                                        gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTAGTTAttt                                                                                       >  2:5756493/1‑128 (MQ=255)
                                         tAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTAT‑‑‑TAATGTAAATATAAA‑TTTAAGTTAttt                                                                                       >  1:6985424/1‑128 (MQ=255)
                                          aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATTAT‑‑T‑TAAATATAAA‑TTTTattttattt                                                                                      >  2:7086799/1‑120 (MQ=255)
                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTAGtttttttttt                                                                                   >  1:888678/1‑128 (MQ=255)
                                               aataaaaattTTATATTTTTTGTTTTTTTATTTATT‑ATTAAA‑TTTTTAAAAAAATAATTTTTACTTTTAAAAAAAA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTtgttgt                                                                                 <  2:6762646/126‑1 (MQ=255)
                                                   aaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTATTAAAATTTAAATATAAA‑TTTTATATATTTTTTTTTATaa                                                                             >  1:1703335/1‑128 (MQ=255)
                                                     actaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTATTTTTTTTTTTGTCTatat                                                                           >  1:5391790/1‑125 (MQ=255)
                                                      attttatTTTTTTATATTTTTTTTGTATT‑TGTAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGt                                                                           <  2:1727871/125‑1 (MQ=255)
                                                       tattatCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTAGGTATTTTTTTTGATAAGTaa                                                                         >  1:6207956/1‑127 (MQ=255)
                                                       tattatCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑c                                                                       <  1:2334967/128‑1 (MQ=255)
                                                       tattaaTTTTTTATATATTTTTTTTTTA‑TTAAAA‑TTTTTAAAAAATTAAATTTAAATTTTAAAAAA‑‑‑‑TTTCTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑c                                                                       <  2:3489098/128‑1 (MQ=255)
                                                        attatCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTATGTAT‑Ct                                                                      >  1:5673620/1‑128 (MQ=255)
                                                         ttatCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTAAAATTGAAATATAAA‑TTTTATGAATTTTTTTTTATAATTaatta                                                                      >  1:3121245/1‑124 (MQ=255)
                                                          tatCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTATTAT‑‑TGAAAAAATAAA‑TTTTAGTAATTTTTTTTTATAATTAtaaa                                                                      >  2:5622252/1‑125 (MQ=255)
                                                           atCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTATTTATTTTTTTTTATAATTaattaa                                                                     >  2:3178391/1‑123 (MQ=255)
                                                             cTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTTTTTTCTAATTAT‑CTaaa                                                                   >  2:6549521/1‑128 (MQ=255)
                                                               ttttATTTATTTTTTTTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATa                                                             <  1:2058687/128‑1 (MQ=255)
                                                               ttttAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGTAAATATAAA‑TTTTAGTTTTTTTGTTGTATAAGTTT‑ATaaaaa                                                                 >  2:900303/1‑128 (MQ=255)
                                                                tttAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑‑TTTTTTTTTTTTATAATGTAAATATAAA‑TTTTATTTATTTTTTTTTATAATTAT‑CTAAAAAAATata                                                           >  1:6081107/1‑126 (MQ=255)
                                                                 ttATATATTTTTTTTATA‑TTCAAA‑TTTTTAAAAAATTAATTTGAAATTGTAAAAAATAG‑‑‑ATTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTaaaaaact                                                              <  2:6962717/128‑3 (MQ=255)
                                                                  tAGATATTTTTTGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTAGTTATTTTTTTTTATAATTAT‑ATAAAAAAATa                                                             >  2:2510103/1‑128 (MQ=255)
                                                                   tttttttttttttttA‑TTTAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAAT‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAg                                                            <  1:2947433/123‑1 (MQ=255)
                                                                   ttattttttttttttA‑TTTAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAg                                                            <  2:3072311/123‑1 (MQ=255)
                                                                    ttttttttttGTTTT‑TTCAAA‑TTTTTATAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGa                                                           <  2:7171465/124‑1 (MQ=255)
                                                                    tattttttttttATT‑TGTAAA‑TTTTTTATAAATTAATTTTTATTTTTTAAAAAT‑‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGa                                                           <  2:4305796/124‑1 (MQ=255)
                                                                            ttGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTT‑‑‑‑TAATGGAAATATAAA‑TTTTATTTATTTTTTTTTGTAATTAT‑ATAAAAAAATATAAAGTaaaaa                                                  >  2:1887078/1‑128 (MQ=255)
                                                                            ttGTATA‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATAAAAT‑TTTTATTTATTTTTTTTTATAATTTTAATAAAAAAATaaattaaaa                                                     >  2:2762519/1‑120 (MQ=255)
                                                                               tata‑TGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTAGTTTTTTTTTTTTATAATTAT‑ATAAAAAAATATAAAGTAAAAA‑at                                               >  2:3385361/1‑128 (MQ=255)
                                                                                 tttagtaaa‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ata                                              <  1:6618513/122‑1 (MQ=255)
                                                                                 ta‑tGAAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGC‑TTTTTTTTTTTTTATTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑at                                               <  1:1969295/128‑1 (MQ=255)
                                                                                    tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTAAATAAAAA‑TTTTATTTTTTTTGTTGTATAATTAT‑ATAAAAAAATAAAAATAAAAAA‑attt                                             >  2:4160593/1‑126 (MQ=255)
                                                                                     gCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTATTTATTTTTTTTTATAAGTAT‑ATAAAAAAATAGAAATGAAAAA‑ATAtttt                                          >  1:4109384/1‑126 (MQ=255)
                                                                                      cAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATTTAAA‑TTTTAGGTTTTTTGTTGTATATGTAT‑CTAATAAAATTTAAATTAAAAA‑ATtttttt                                         >  2:1585965/1‑128 (MQ=255)
                                                                                      cAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTAGGTATTTTTTTTTCTAATTAT‑ATAAAAAAATAGAAAGTAAAAA‑TTATTTTTa                                        >  2:3889953/1‑128 (MQ=255)
                                                                                      aaaa‑TTTTTAAAAAATTTATTTTAATTTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTa                                        <  2:5497852/127‑1 (MQ=255)
                                                                                       aaa‑TTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTa                                        <  1:6306209/128‑1 (MQ=255)
                                                                                        aa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTTGGTATTTTGTTTTATAATTAT‑ATAAAAAAAAAGAAAGGAAAAA‑ATTTTGTTaa                                       >  1:580370/1‑128 (MQ=255)
                                                                                           ttttTAAAAAATTAATTTTTAATTTTAAAAAAT‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTaaaa                                     <  1:2816564/128‑1 (MQ=255)
                                                                                           ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATAAAAA‑TTTTAGTTATTTTTTTGTATAATTAT‑ATAAAAAAATAAAAAGTAAAAA‑ATAATTTTaaaa                                     >  2:2237547/1‑128 (MQ=255)
                                                                                              ataaaaaaTTAATTTTTACTTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTAAAAGt                                   <  1:1340027/126‑1 (MQ=255)
                                                                                               taaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAATGAAAAA‑ATATTTTTAAAAGTaa                                 >  2:1192167/2‑128 (MQ=255)
                                                                                                   aaattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAT‑TTTTATGTATTTTTTTTTATTAGTAT‑ATAAAAAATTATAAAGAAAAAA‑ATTTTTTTAAAATAAATAc                              >  2:5143067/2‑128 (MQ=255)
                                                                                                    aattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTAAAAAT‑GAAAAATAAATTTTTTTGAATTTTGTTGAAT‑ATTAT‑CTAAAAAAATAAAAATGAAAAATATATT‑TTAAAATTaaaaaga                            >  1:4963244/1‑123 (MQ=255)
                                                                                                    aattaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTAGGTATTTTTTTGAGTAAGTAT‑CTAAAAAAATTGAAAGTAAAAA‑AATTTTTTAAAAATATTAATa                            >  2:3747283/1‑128 (MQ=255)
                                                                                                     attaattTGTATTTTTAAAAAATATC‑TTTTTTTTTTTTTTTTATATAATGGAAATATAAA‑TTTTGGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTAAAAGTAATACt                             <  2:6072872/128‑1 (MQ=255)
                                                                                                     attaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAA‑TTTTAGTATTTTTGTTGTATAATTTT‑AAAAAAAAATATAATGTAAAAA‑ATATTTTTAAAATtaataataa                           >  1:4519909/1‑128 (MQ=255)
                                                                                                     attaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGGAAATATAAA‑TTTTATGTATTTTTTTGTATAATTAT‑CTAAAAAAATAGAAAGTAAAAA‑ATATTTTTAAAATTATTACTaa                           >  1:3957989/1‑128 (MQ=255)
                                                                                                     attaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTATAAGTAT‑ATAAAAATATAGAAAGGAAAAA‑ATATTTTTAAAATTAATAATaaa                          >  2:1071761/1‑128 (MQ=255)
                                                                                                      ttaattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATATTGTAATTATAAA‑TTTTAGGTTTTTTGTTTTGTTATTTT‑ATAAAAATATAGAAAGTAAAAT‑ATTTTTTTaaatttattaata                            >  2:5361345/1‑118 (MQ=255)
                                                                                                      ttaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTTTATAAGTAT‑ATAAAAAAATATAAAGTAAAAA‑ATTTTTTTAAATGTATTACTaaa                          >  2:3645425/1‑128 (MQ=255)
                                                                                                      ttaattTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGTTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGTAAAAA‑ATATTTTTAAAATTAATAATAAAat                        >  1:4367537/1‑128 (MQ=255)
                                                                                                      ttaattTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGTTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAAAAAGGAAAAA‑ATATTTTTAAAATTAATACTAAAat                        >  2:5280092/1‑128 (MQ=255)
                                                                                                      ttaattTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTATAAGTAT‑CTAAAAAAATATAATGGAAATA‑ATTTGTTTAAAAGTAATACTAAAat                        >  2:6409121/1‑128 (MQ=255)
                                                                                                       taattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTTGGTATTTTGTTGTCTAATTTT‑CTAAAAAAATAGAAAGTAAAAA‑ATATTGTTAAAAGTAATAATTAAat                        >  1:1148812/1‑128 (MQ=255)
                                                                                                        aattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAA‑TTTTAGTTATTTTGTTTTATAATTAT‑ATAAAAAAATATAAAGGAAAAA‑ATATTTTTAAAATTAATAATAAAat                        >  2:2665605/1‑128 (MQ=255)
                                                                                                            tGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTATAATTAT‑ATAAAAAAATATAAAGGTAAAA‑ATATTTTTAAAAGTAATAATAAAattttt                    >  2:2359311/1‑128 (MQ=255)
                                                                                                                cTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTTTAAAATTAT‑ATAAAAAAATAGAAAGTAAAAA‑ATATTTATAAAATTAAAAATAAAATTATTTAAt                >  2:4460940/1‑128 (MQ=255)
                                                                                                                  tttAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTAAAAGTAATACTAAAATTATTTAATGt              <  2:2855281/126‑1 (MQ=255)
                                                                                                                  tGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGAATTTTGTTTTCTAATTAT‑CTAAAAAAATAAAAAGGAAAAA‑ATATTTTTAAAAGTAAAACTAAAAATATTTAATGt              >  2:6867301/1‑128 (MQ=255)
                                                                                                                   gTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGAAAATAAAAA‑TTTTTGGTTTTTTGTTGTATAATTAT‑AAAAAAAAATAAAAAGTAAAAA‑ATATTTTTAAAATTAATAATAAAATAATTTAAttt              >  1:3251608/1‑126 (MQ=255)
                                                                                                                     gtaaaaaatttgtttttttttttttttttATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTAAAAGTAATACTAAAATTATTTAATGt              <  1:7119610/116‑1 (MQ=255)
                                                                                                                      ttaataaatattttttttttttttttttATAATGTAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTAAAAGTAATACTAAAATTATTTAATGtt             <  1:4272305/118‑1 (MQ=255)
                                                                                                                        aataatttttttttttttttttttttATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTAAAAGTAATACTAAAATTATTTAATGttaa           <  1:1005385/121‑1 (MQ=255)
                                                                                                                            tagcttttttttttttttttttATAATGGATTTTAAAA‑TTTTAGTTATTTTGTTGTTTATTTAT‑ATAAAAAAATATTAAGGAAATA‑ATATTTTAAAATTTAATAATAAATTTTTTTAATAttatataa       >  2:4630756/5‑123 (MQ=255)
                                                                                                                                 |                                                                                                                                  
AATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAA‑TTTTAGGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAA‑ATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTT  >  CP016816/165125‑165379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑