BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	202,485	0	T	.	73.2%	469.7 / inf	448	intergenic (+263/‑412)	JCVSYN2_00840/JCVSYN2_00845	hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand): ref base T (28/75); new base . (120/162); total (181/267)
Fisher's exact test for biased strand distribution p-value = 6.47e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-02

Only 100 of 497 total aligned reads displayed.

GAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCCTTTTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATA  >  CP016816/202357‑202606

 aaGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATAACATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCCt                                                                                                                             <  2:5537549/128‑1 (MQ=255)

  aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCCtt                                                                                                                            <  1:2882142/128‑1 (MQ=255)

  aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCACtt                                                                                                                            >  1:5517191/1‑128 (MQ=255)

   gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCT‑ATTTTTTTA‑TTTTTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAAACACCtaa                                                                                                                           >  1:841574/1‑126 (MQ=255)

   gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATATTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCACtaa                                                                                                                           >  2:6288051/1‑126 (MQ=255)

    ttattttAAAAAATATATTTAATTTTAATTAAAATTACATAAAATTTATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTGGTTTATATT‑AAC‑AAAT‑AAAAAAAAAACAATCCCCttaa                                                                                                                          <  1:4259025/122‑3 (MQ=255)

     tAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTAATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCCttaaa                                                                                                                         >  1:7130488/1‑125 (MQ=255)

      aGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCAttaaaa                                                                                                                        >  1:6998864/1‑124 (MQ=255)

         gtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCCtaaaaaaat                                                                                                                     >  1:1738215/1‑120 (MQ=255)

             ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCACC‑‑Ttaaa‑‑aattaaaa                                                                                                             >  2:6122398/1‑128 (MQ=255)

              taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTCTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAAACCCC‑‑ttaaa‑‑aattaaaaa                                                                                                            >  1:6611572/1‑128 (MQ=255)

              taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTATTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑ttaaa‑‑aattaaaaa                                                                                                            >  2:5913687/1‑128 (MQ=255)

                 taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCT‑ATTTTTATA‑TTTGTTAATCTT‑CAC‑AAAA‑AAAAAAAAAACAAT‑‑CCAATAAAA‑‑AAATaaaaaaaa                                                                                                         >  2:3629491/1‑128 (MQ=255)

                 taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTTTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCAC‑‑TTAAA‑‑AATTaaaaaaat                                                                                                         >  1:3410534/1‑127 (MQ=255)

                 taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAAAAATACCC‑‑TTAAA‑‑AATTaaaaaaaa                                                                                                         >  1:5440942/1‑128 (MQ=255)

                    tgttaatgttaatTAAAAATACATAAAATTAATAATTTTTTTAATTTTCTTCCTCGTGATCT‑ATTTTTTTA‑TTTTTTTATCTT‑AAA‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaa                                                                                                      <  1:5486431/127‑1 (MQ=255)

                    atttAATGTTAATTAAAAAACAAAAAAATTAAAAATTCATATAATTTATATCCACGTTATAT‑ATTTTTTTA‑TTTGTTTATATT‑AAA‑AAAA‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaa                                                                                                      <  2:669251/126‑1 (MQ=255)

                    agTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTATTTA‑TTTTTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATACCC‑‑TTAAA‑‑AATTaaaaaaaaaaa                                                                                                      >  1:914773/1‑128 (MQ=255)

                      ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATTTATATACTCAATATCT‑AATTTTATA‑AAAAAAAATCTT‑AAA‑AAAA‑AAAAAAAAAAAAATAAC‑‑‑AAAAA‑‑AAATaaaaaaaaaaaaaa                                                                                                   >  1:5119821/1‑128 (MQ=255)

                       tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑‑AAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaa                                                                                                  >  1:7028118/1‑128 (MQ=255)

                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCT‑ATTTTTTTA‑TTTTTTTATCTT‑CAA‑AAAT‑AAAAAAAAAACAATACCATT‑‑‑AA‑‑AAATaaaaaaaaaaaaaaaa                                                                                                 >  2:787965/1‑128 (MQ=255)

                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTTTTTATATT‑AAA‑AAAT‑AAAAAAAAAAAAATCCAAATaaaaa‑‑aaaaaaaaaaaaaaaaa                                                                                                    >  1:3132969/1‑128 (MQ=255)

                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑‑AAAAAAAAACAATCCCC‑‑‑TAAA‑‑AAATtaaaaaaaaaaaaaaaa                                                                                                >  1:5813160/1‑128 (MQ=255)

                         aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCAGC‑‑TAAAA‑‑AAATaaaaaaaataaaataa                                                                                                 >  1:6124841/1‑128 (MQ=255)

                         aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAAACCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaa                                                                                                 >  1:493354/1‑128 (MQ=255)

                         aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCATCGTGATCT‑ATTTTTTTA‑TTAGTTTATCTA‑CAC‑AAAT‑AAAAAAAAAACAATAACC‑‑TTAAA‑‑AAATaaaaaaaaaaaaaaaa                                                                                                 >  2:5991862/1‑128 (MQ=255)

                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATACCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaa                                                                                                >  1:769337/1‑128 (MQ=255)

                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAAAACCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaa                                                                                                >  1:2465398/1‑128 (MQ=255)

                           gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCT‑ATTTTTTTA‑TTTTTTTATCTT‑AAC‑AAAT‑AAAAAAAAAAAAAT‑‑‑AAAATAAA‑‑AAATaaaaaaaaaaaaaaaaaaa                                                                                              >  1:2286/1‑128 (MQ=255)

                           gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAAAAA‑‑AACAATTAAA‑‑AATTaaaaaaaaaaaaaaaaaa                                                                                               >  2:3541019/1‑128 (MQ=255)

                           gTTAATTAAAAAACCATAAAATTAATAATTATTATAATTTATATAATTGTTTTTT‑ATTTTTTTA‑TTTGTTTATATT‑AAA‑AAAA‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaa                                                                                               <  2:6177513/128‑1 (MQ=255)

                            ttaaataaAAAAACATAAAATAAAAAAAAATTAAAATTTATAACCTACTTATAT‑ATTTTTTTA‑TTTTTTTATATT‑AAA‑AAAT‑AAAAAAAAAAAAAACCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaaa                                                                                              <  2:3314831/128‑1 (MQ=255)

                             taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTTTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATAAACAT‑‑‑AA‑‑AAATaaaaaaaaaaaaaaaaaaaaa                                                                                            >  1:2096177/1‑128 (MQ=255)

                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaa                                                                                            >  1:6195632/1‑128 (MQ=255)

                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAAACCCATTAAAAA‑‑Taaaaaaaaaaaaaaaaaaaaaa                                                                                              >  2:2647560/1‑128 (MQ=255)

                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAAAAAC‑‑‑ATAAA‑‑AAATaaaaaaaaaaaaaaaaaaaaaa                                                                                           >  2:6543912/1‑127 (MQ=255)

                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTATTA‑TTTGTTTATCTT‑CAA‑AAAT‑AAAAAAAAAACAATAAC‑‑ATAAAA‑‑AAATaaaaaaaaaaaaaaaaaaaaa                                                                                            >  2:2274444/1‑128 (MQ=255)

                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCATCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAATAAAAAAAAAAACAAACCAC‑‑‑TAAA‑‑AAATtaaaaaaaaaaaaaaaaaaaa                                                                                            >  1:6469794/1‑128 (MQ=255)

                              aattaaAATACCATAAACTTAATAATTATT‑TAATGTAGATCATCTTTATCT‑ATTTTTTTA‑TTTTTTTATCTT‑CAA‑AAAT‑AAAAAAAAAAAAAAACAC‑‑TAAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaa                                                                                           >  2:1916638/1‑127 (MQ=255)

                               attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCACC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaa                                                                                           >  1:5734649/1‑127 (MQ=255)

                               attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTATTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAAACC‑‑‑AATAAA‑‑AAATaaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:1583326/1‑126 (MQ=255)

                                ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTTTTTATCAT‑CAC‑AAAT‑AAAAAAAAAAAAA‑‑AACAATTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:6490392/1‑126 (MQ=255)

                                ttaaAATACCATAAACTTAATAATTATTATAATCTAAATAAACTTGATCT‑ATTTTTATA‑TATATTTATATT‑CAC‑AAAT‑AAAAAAAAAAAAA‑‑AACAAATAAA‑‑AAATaaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:2645524/1‑126 (MQ=255)

                                ttaaAAAAACATAAACTTAATAATTATTAATATTTATTTCCTCGTGATCT‑ATTTTTTTT‑TTTTTTTATCTT‑AAC‑AAAA‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaac                                                                                          <  2:4332857/128‑3 (MQ=255)

                                 taaaataaaataaaaTTAAAAAATAATAAAATGTTTATCCACGTGTTCTAATTTTTTTA‑TTT‑TTTATCTT‑AAC‑AAAT‑AAAAAAAAAACAAACCCC‑‑ATAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTa                                                                                         <  2:6899295/128‑1 (MQ=255)

                                  aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                        >  1:3629082/1‑124 (MQ=255)

                                  aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCACTTAAAAA‑‑Taaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:4120590/1‑126 (MQ=255)

                                  aaaaTACCATAAACTTAAAAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAAACACCATAAAAA‑‑Taaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:4610886/1‑126 (MQ=255)

                                   aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AAATaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                       >  1:6899141/1‑123 (MQ=255)

                                   aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAAAAATACC‑‑ATTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                       >  1:103862/1‑123 (MQ=255)

                                    aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTTTTTATCTT‑CAC‑AAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaataaaaaa                                                                                      >  1:5696405/1‑121 (MQ=255)

                                      taaaaTAAAATTAAAAATTATTTTAATTTATATACTAGTTATAT‑ATTTTTTTA‑TTTTTTTATATT‑AAA‑AAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAaaac                                                                                    <  2:5651337/124‑1 (MQ=255)

                                      tACCAAAAAATTAAAAATTCTTATAATTTATATCCTTGGTATTT‑ATTTTTTTA‑TTTTTTTTTATT‑AAA‑AAAA‑AAAAAAAAAAAAAACCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAaaac                                                                                    <  1:6262784/128‑1 (MQ=255)

                                       aCCATAAAATTAAAAATTATTTTAATGTTGATACGTTTTATTT‑ATTTTTTTT‑TTTTTTTATTTT‑CAC‑AAAA‑AAAAAAAAAAAAAACCCC‑‑TTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                   <  1:4020980/128‑1 (MQ=255)

                                        caaaaaaaTTAATAATATTTATAATTTATAATCACTTTTTAT‑ATTTTTTTT‑TTTGTTTATATT‑CAA‑AAAT‑AAAAAAAAAAAAAAACCC‑TTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                   <  2:6357242/124‑1 (MQ=255)

                                        aaaTAAAATTAATAATTATTATAATTTAATAAATTCTTATAT‑ATTTTTTTA‑ATTTTTTAAATT‑AAA‑AAAA‑AAAAAAAAAAAAAACCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                  <  2:2363935/126‑1 (MQ=255)

                                         caaaaaCTTAATAATTAATAAAATTTATAATCACGTTATAT‑AATTTTTTA‑TTTTTTTATATT‑AACAAAAA‑AAAAAAAAAACAAACCCC‑TTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                   <  2:4438101/125‑1 (MQ=255)

                                           tAAAATTAATAATTTTTAAAATTTAGATCATCGTTATAT‑ATTTTTTTT‑TTTTTTTATCTT‑TAA‑AAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑AA‑‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                             <  1:5956020/128‑1 (MQ=255)

                                             aaattAATTATTTATATAATTTACTTCATCGTTATAT‑ATTTTTTTT‑TTTGTTTATCTT‑AAC‑AAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                            <  1:5624151/125‑1 (MQ=255)

                                             aaCTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGATTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TAAAA‑‑AATTAAAAAAAAATAAACAAAAAAACAAACAAAAAActaa                                                                             >  1:6393316/1‑126 (MQ=255)

                                             aaCTTAATAATAAATAAAATTTATAACATCGTCATCT‑ATTTTTTTA‑ATTGTTTATCAT‑CAA‑AAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                            <  2:1540460/128‑1 (MQ=255)

                                              aaataaaaaTTATTAAAATATAAATCCTAGTTTTTT‑ATTTTTTAA‑ATTTTTTAAAAT‑AAA‑AAAA‑AAAAAAAAAAAAAACCC‑‑ATTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                            <  2:4173170/125‑1 (MQ=255)

                                                       ttattAAAATTTAGATCCTCGTTATAT‑ATTTTTTTA‑TTTTTTTATATT‑AAC‑AAAA‑AAAAAAAAAACAATCACC‑TTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaa                                                                    <  2:403655/125‑1 (MQ=255)

                                                                  tttataatttttaTCT‑ATTTTTTTA‑TTTTTTTATATT‑AAA‑AAAT‑AAAAAAAAAAAAATCCCCAT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                       <  2:50933/116‑1 (MQ=255)

                                                                     acctgcttctctt‑attttttta‑ttttttTA‑ATT‑AAA‑AAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTCTTTCTTAAAATTCCCTATTATACca                                                   <  2:6840175/116‑1 (MQ=255)

                                                                     aaacacGTTATTT‑ATTTTTTTA‑TTTTTTTATATT‑AAA‑AAAA‑AAAAAAAAAAAAAACCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                    <  2:905702/123‑1 (MQ=255)

                                                                       actaattaTCT‑ATTTATTTA‑TTTTTTTATATT‑AAA‑AAAA‑AAAAAAAAAAAAAAACC‑‑ATTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                   <  2:1228483/121‑1 (MQ=255)

                                                                        cacttttaat‑ATTTTTTTA‑ATTTTTAATATTAAAA‑AAAA‑AAAAAAAAAAAAAACCCCATAAAAA‑‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                     <  2:3897685/119‑1 (MQ=255)

                                                                         cttttttat‑atttttttattttttTTATTTA‑AAC‑AAAA‑AAAAAAAAAAAAATACCCTTT‑‑AA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                  <  1:3809433/120‑1 (MQ=255)

                                                                         ctattttttatttttttta‑tttttttttatt‑AAA‑AAAA‑AAAAAAAAAAAAAACCCATTAAAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                    <  2:5426165/117‑1 (MQ=255)

                                                                         aattttaat‑attatTTTA‑ATTTTTTTTATA‑AAA‑AAAA‑AAAAAAAAAAAAATCCCC‑TAAAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                  <  2:283227/120‑1 (MQ=19)

                                                                             ttttttttttttttA‑TTT‑TTTATATT‑TAA‑AAAA‑AAAAAAAAAAAAAAACC‑‑ATTAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                             <  2:4996227/121‑1 (MQ=255)

                                                                             tttat‑ATTTTTTTT‑TTTTTTATTATA‑TAA‑AAAA‑AAAAAAAAAAAAAAACCC‑‑ATAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                             <  2:548641/124‑1 (MQ=255)

                                                                              ttttatttttttta‑tttttttaTTTT‑AAA‑AAAT‑AAAAAAAAAAAAAACCCC‑ATAAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                              <  1:6095677/122‑1 (MQ=255)

                                                                              tatatttttttttt‑tttttttttttt‑AAA‑AAAA‑TAAAAAAAAAAAATACAC‑TTAAAA‑‑TATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                              <  1:5991862/122‑1 (MQ=255)

                                                                                tttaattttttt‑tttttttATATT‑AAA‑AAAA‑AAAAAAAAAAAAAAAACCATAAAAA‑‑TAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                             <  1:6303828/123‑1 (MQ=255)

                                                                                   tttttttta‑tttttttttatt‑AAA‑AAAA‑AAAAAAAAAAAAAAACAC‑TTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttattt                                         <  1:1916638/127‑1 (MQ=255)

                                                                                    tttttttt‑ttttttTATAAT‑TAA‑AAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                      <  2:2991353/128‑1 (MQ=255)

                                                                                    atttttaT‑TTTTTTTATAAT‑AAA‑AAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                      <  2:1356920/127‑1 (MQ=255)

                                                                                     ttatttA‑TTTTTTTAAAAT‑AAA‑AAAA‑AAAAAAAAAAAAAT‑ACCATTAAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                       <  2:7125456/125‑1 (MQ=255)

                                                                                       ttttt‑tttttttATATT‑TAA‑AAAT‑AATAAAAAAAAAAAAACATTAAAAA‑‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                      <  2:3120467/128‑1 (MQ=255)

                                                                                       ttttA‑TTTTTTTATATT‑AAA‑AAAT‑AAAAAAAAAAAAATACAC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                    <  2:2585224/128‑1 (MQ=255)

                                                                                       ttttA‑ATTTTTTATAAT‑AAC‑AAAA‑AAAAAAAAAAAAATACCC‑ATAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                     <  2:6084564/128‑1 (MQ=255)

                                                                                             ttttttTATATT‑AAA‑AAAA‑AAAAAAAAAAAAATCAC‑‑ATTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                               <  2:1465921/128‑1 (MQ=255)

                                                                                                  ttttttatttaaaaaT‑AAAAAAAAAAAAAAACCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                            <  2:2411851/116‑1 (MQ=255)

                                                                                                  atttttaataaaaaaT‑AAAAAAAAAAAAATACCATAT‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                            <  2:4834031/116‑1 (MQ=255)

                                                                                                    tatttttaaaaaaT‑AAAAAAAAAAAAATACC‑‑ATTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCACTATTATACCACATTTTATTTTTAAAATAAGATAtt                          <  2:5250119/118‑1 (MQ=255)

                                                                                                     aaat‑CAA‑AAAA‑AAAAAAAAAAAAAAACCCTTTAAAATTAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGatat                           <  2:5825001/125‑1 (MQ=255)

                                                                                                      cTT‑CAC‑AAAT‑‑AAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAA‑AAAAAACAAAATATATTTATTAAAATTACAAATTTAACCAAATTTTTTTTTTAAAAAAAAATATTGAAata                    >  2:6268826/1‑128 (MQ=255)

                                                                                                       aattaaaaaaa‑aaaaaaaaaaaaATACCC‑‑ATAAA‑‑AAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                      <  2:3435100/121‑1 (MQ=255)

                                                                                                        attaaaaaaa‑aaaaaaaaaaaaATACCATT‑‑‑AA‑‑AAATT‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                    <  1:3548446/122‑1 (MQ=255)

                                                                                                          attaaaaa‑aaaaaaaaaaaaaaaaCCCATTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                      <  2:4512229/124‑1 (MQ=255)

                                                                                                           taaaaaa‑aaaaaaaaaaaaaaCCCC‑‑TTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataa                   <  2:1776429/125‑1 (MQ=255)

                                                                                                           aaaaaaa‑aaaaaaaaaaaaATCACC‑ATAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                    <  2:7026761/125‑1 (MQ=255)

                                                                                                            aaaaaa‑aaaaaaaaaaaaaaaaCCCTTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                    <  2:6435599/126‑1 (MQ=255)

                                                                                                             aaaaa‑aaaaaaaaaaaaATACC‑‑ATTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata                 <  1:6735849/127‑1 (MQ=255)

                                                                                                              aaaT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAAAAAAACCCAACAATTATTTATAAAAAATACAAAATTTACCAAAATTTATTTTTTAAATAATAAATTGACAAAatat                >  2:3757588/1‑128 (MQ=255)

                                                                                                                 aaaaaaaaaaaaCAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatata             <  2:2088807/126‑1 (MQ=255)

                                                                                                                         aaaaCAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAA‑‑TAAAAACAAACGATTTTTATTAAAATTCCCTTTTTAACCACATTTATTTTTTAAAATAAGATTTTGAATAAaaatatataaaaattat   >  1:3711026/1‑119 (MQ=255)

                                                                                                                           aaCAATCCCC‑TTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACAAAAAACAAACTATATTTATTAAAATAGCAATTTTAACCAAATTTTTTTTTTAAAATAATATATTGAAATAATTTATTTATAaaat     >  2:5731556/1‑128 (MQ=255)

GAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCT‑ATTTTTTTA‑TTTGTTTATCTT‑CAC‑AAAT‑AAAAAAAAAACAATCCCCTTTTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATA  >  CP016816/202357‑202606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`