BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	369,745	C→T	Q76* (CAA→TAA)	JCVSYN2_01605 →	LemA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	369,745	0	C	T	99.7%	9742.3 / NA	3100	Q76* (CAA→TAA)	JCVSYN2_01605	LemA family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (1328/1759); minor base A (8/2); total (1338/1762)
Fisher's exact test for biased strand distribution p-value = 2.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3100 total aligned reads displayed.

CATTAGTTTTTGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCACAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGATCTGGAATTAA  >  CP016816/369620‑369869

cATTAGTTTTTGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATaa                                                                                                                            <  2:2435246/128‑1 (MQ=255)

 aTTAGTTTTTGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATaaa                                                                                                                           <  2:1473350/128‑1 (MQ=255)

  ttAGTTTTTGAAATACTATTATGAATATTAGGAATTATTCATGGAATTATTTTTTCATTTATAAAAATTAATTCTAAAAATCATTAAGATCAACTAGAACAAAAAGTAAAACACAATGCTTCATAAAt                                                                                                                          >  2:1994733/1‑128 (MQ=255)

     gTTTTTGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTTCATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAATAAGTACAACACAATGCTTCATAAATTGa                                                                                                                       >  1:7148354/1‑128 (MQ=255)

     gTTTTTGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTATCTCAACTAGAACAAAAAGTACAACACAATGCTACATAAATTGa                                                                                                                       >  2:6736271/1‑128 (MQ=255)

     gTTTTTGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGa                                                                                                                       >  1:4026308/1‑128 (MQ=255)

     gTTTTTGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGa                                                                                                                       >  2:6664316/1‑128 (MQ=255)

      tttttGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGAt                                                                                                                      >  2:2326562/1‑128 (MQ=255)

           gAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTa                                                                                                                 <  1:4965643/128‑1 (MQ=255)

           gAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTa                                                                                                                 <  1:1438973/128‑1 (MQ=255)

           gAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTa                                                                                                                 <  2:6157936/128‑1 (MQ=255)

           gAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTa                                                                                                                 >  2:4201880/1‑128 (MQ=255)

            aaaTACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTAt                                                                                                                <  1:5263480/128‑1 (MQ=255)

             aaTACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTATCACAAAAAGTACAACACAATGCTTCATAAATTGAGAACTAtg                                                                                                               >  1:1654093/1‑127 (MQ=255)

               tACTATTATGAATATTAGGAATTATTCCTGGAATTTTTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTa                                                                                                             <  1:5612241/128‑1 (MQ=255)

                  tattatGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGAt                                                                                                          <  1:115652/128‑1 (MQ=255)

                  tattatGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGATCAACTAGAACAAAAATTACAACACAATGCATCATAAATTGATAACAATTTACAt                                                                                                          >  1:4344904/1‑128 (MQ=255)

                  tattatGAATATTAGGAATTATTCCTGGAATTATTTATACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGAt                                                                                                          <  2:589964/128‑1 (MQ=255)

                   attatGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATc                                                                                                         >  1:3196458/1‑128 (MQ=255)

                   attatGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATc                                                                                                         <  2:652917/128‑1 (MQ=255)

                    ttatGAATATTAGGAATTATTCCTGGAATTATTTTTACTTTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGATCAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCa                                                                                                        <  2:7025702/128‑1 (MQ=255)

                    ttatGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGAACAACACAATGCTACATAAATTGATAAATATTTAGATCa                                                                                                        >  1:1764857/1‑128 (MQ=255)

                     tatGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCaa                                                                                                       <  1:3461185/128‑1 (MQ=255)

                       tGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAag                                                                                                     <  2:1308261/128‑1 (MQ=255)

                        gAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAaga                                                                                                    >  2:6879751/1‑128 (MQ=255)

                              tAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGTCTAAATATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCtgtt                                                                                              <  2:2674742/128‑1 (MQ=255)

                              tAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCtgtt                                                                                              <  2:3137399/128‑1 (MQ=255)

                               aGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCtgttg                                                                                             <  1:6623288/128‑1 (MQ=255)

                               aGGAATTATTCCTGGAATAATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCtgttg                                                                                             <  2:4407582/128‑1 (MQ=255)

                                  taatattCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACACCACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCAGTTGATa                                                                                          >  2:4537896/4‑128 (MQ=255)

                                   attattCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTAt                                                                                         <  1:6892867/128‑1 (MQ=255)

                                      attCATGGAATTATTTTTACATTTATAAAAATTAAGGATAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCa                                                                                      <  1:154957/128‑1 (MQ=255)

                                       ttCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCaa                                                                                     >  1:3876941/1‑128 (MQ=255)

                                          cTGGAATTATTTTTACATTTATAACAATTAACGCTAATAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAAt                                                                                  <  2:431438/128‑1 (MQ=255)

                                           tGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAAtt                                                                                 >  2:4056206/1‑128 (MQ=255)

                                             gAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTa                                                                               <  1:650164/128‑1 (MQ=255)

                                              aaTTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCtgttgt                                                                                            >  1:4590870/1‑114 (MQ=255)

                                              aaTTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAg                                                                              >  1:1006036/1‑128 (MQ=255)

                                               attattTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGc                                                                             <  2:2551499/128‑1 (MQ=255)

                                                 tattTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCtt                                                                           <  2:526308/128‑1 (MQ=255)

                                                 tattTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCtt                                                                           <  2:3423498/128‑1 (MQ=255)

                                                     tttACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCatt                                                                       <  1:5872892/128‑1 (MQ=255)

                                                       tACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCattat                                                                     >  2:1591794/1‑128 (MQ=255)

                                                       tACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCattat                                                                     <  2:5115838/128‑1 (MQ=255)

                                                         cATTAATAAAAATTAATGATAAAAATCATTTATCTAAACTATAAAAAAAAGTACAACAAAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCAttatta                                                                   <  2:182261/128‑1 (MQ=255)

                                                          aTTTATAAAAATTAATGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAAATATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATtattat                                                                  <  1:4167199/128‑1 (MQ=255)

                                                           tttATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATc                                                                 <  2:2791298/128‑1 (MQ=255)

                                                             tataAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCaaaa                                                                                   <  1:7001914/108‑1 (MQ=255)

                                                             tataAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTa                                                               <  1:5732624/128‑1 (MQ=255)

                                                              ataAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTaa                                                              <  1:6365439/128‑1 (MQ=255)

                                                              ataAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTaa                                                              <  1:2057395/128‑1 (MQ=255)

                                                              ataAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTaa                                                              >  2:496258/1‑128 (MQ=255)

                                                               taAAACTTAATCCTAAAAATCATTTAGCTCAAGATGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTaaa                                                             <  1:1320928/128‑1 (MQ=255)

                                                                    aTTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAAttt                                                                                <  2:1169021/104‑1 (MQ=255)

                                                                     ttAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAAtt                                                       <  2:4798698/128‑1 (MQ=255)

                                                                      tAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTg                                                      <  2:3911740/128‑1 (MQ=255)

                                                                        atgCTAAAAATCATTTAGCTCAACTAGAAAAAAAAGTACAAAAAAATTCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGaa                                                    <  1:3728623/126‑1 (MQ=255)

                                                                        aGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTAATTATTATCTAAATCAATTGaa                                                    >  1:2705324/1‑128 (MQ=255)

                                                                         ggCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAAc                                                   <  1:2327281/128‑1 (MQ=255)

                                                                         ggCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAAc                                                   <  1:4806213/128‑1 (MQ=255)

                                                                          gCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACt                                                  <  2:2919294/128‑1 (MQ=255)

                                                                            tAAAAATCATTTAGCTCAACTAGAACAAAAAGTCCAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAg                                                <  1:5970873/128‑1 (MQ=255)

                                                                             aaaaaTCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGGTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGATCTTGa                                               >  2:3693279/1‑128 (MQ=255)

                                                                              aaaaTCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAAc                                                   >  2:6285299/1‑123 (MQ=255)

                                                                              aaaaTCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGAt                                              >  2:640930/1‑128 (MQ=255)

                                                                              aaaaTCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGAt                                              >  2:866754/1‑128 (MQ=255)

                                                                              aaaaTCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATAGAACGAGAt                                              >  2:2181395/1‑128 (MQ=255)

                                                                                aaTCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATaa                                            <  1:74640/128‑1 (MQ=255)

                                                                                     tttAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATg                                       <  1:2570538/128‑1 (MQ=255)

                                                                                      ttAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGt                                      >  2:6035453/1‑128 (MQ=255)

                                                                                       tAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGtt                                     >  1:4457592/1‑128 (MQ=255)

                                                                                         gCTCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAAc                                                   >  1:6105654/1‑112 (MQ=255)

                                                                                          cTCAACTAGAACAAAAAGTTCAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATg                                  >  2:3264245/1‑128 (MQ=255)

                                                                                          cTCAAATAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATg                                  <  1:4691550/128‑1 (MQ=255)

                                                                                           tCAACTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGa                                 >  2:75769/1‑128 (MQ=255)

                                                                                              aCTAGAACAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAAATGAAGTAGATAAATATGTTATGaaaa                              >  1:1886354/1‑128 (MQ=255)

                                                                                                tAGAACAAAAAGTAGAACACAATGTTTCATAAATTGATTAGTATTTAGATCATAGTGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTTAATCAATTGAACTAGATAAAGATGTTATGAAAACa                            <  1:3088734/128‑1 (MQ=255)

                                                                                                   aaCAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGATAAATTTAGCTTCATAATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGtt                         >  1:2945324/1‑128 (MQ=255)

                                                                                                    aCAAAAAGTACCACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAATCAGTTg                        >  1:5125893/1‑128 (MQ=255)

                                                                                                    aCAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTg                        <  2:3565835/128‑1 (MQ=255)

                                                                                                    aCAAAAAGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTg                        <  1:908447/128‑1 (MQ=255)

                                                                                                       aaaaGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAg                     <  2:5512163/128‑1 (MQ=255)

                                                                                                         aaGTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCt                   >  1:2833796/1‑128 (MQ=255)

                                                                                                           gTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTta                 >  1:6272102/1‑128 (MQ=255)

                                                                                                           gTACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCAGTATTATCTAAATCAATTGAACTAGATAAAGATTATATGAAAACAGTAGCAGCTta                 >  2:562321/1‑128 (MQ=255)

                                                                                                            tACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTtat                <  1:2193125/128‑1 (MQ=255)

                                                                                                            tACAACACAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTGGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTtat                <  2:1715931/128‑1 (MQ=255)

                                                                                                            tACAACACAATGCTGCATAAATTGATATCTTTTTAGAGCAATGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTtat                <  1:6745652/128‑1 (MQ=255)

                                                                                                             acaacaCAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTtata               <  2:539999/128‑1 (MQ=255)

                                                                                                                acacAATGCTTCATAATTTGATTACTATTTAGATCAAAGAGCTGTTGTTATGCAATATTTAGCTTCATTATTATCTAAATCAATTGATCTAGATAATGATGTTATGAAAACAGTTGCAGCTTATAGAt            <  1:2444400/128‑1 (MQ=255)

                                                                                                                acacAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAATTTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGAt            >  1:3572653/1‑128 (MQ=255)

                                                                                                                acacAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGAt            >  2:3034955/1‑128 (MQ=255)

                                                                                                                acacAATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGAt            <  1:3329810/128‑1 (MQ=255)

                                                                                                                   caATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGATCTg         <  2:10106/128‑1 (MQ=255)

                                                                                                                   caATGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGATCTg         <  2:5754160/128‑1 (MQ=255)

                                                                                                                    aaTGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATACAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGa                                 <  1:5498408/103‑1 (MQ=255)

                                                                                                                     aagCTTCATATATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGATCTGGa       <  2:3798631/126‑1 (MQ=255)

                                                                                                                     aTGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGATCTGGa       <  1:5381843/128‑1 (MQ=255)

                                                                                                                      tGCTTCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTg                        <  2:7148860/110‑1 (MQ=255)

                                                                                                                          tCATAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCTGCTGATAGATCTGGATTTaa  >  2:4691380/1‑128 (MQ=255)

CATTAGTTTTTGAAATACTATTATGAATATTAGGAATTATTCCTGGAATTATTTTTACATTTATAAAAATTAAGGCTAAAAATCATTTAGCTCAACTAGAACAAAAAGTACAACACAATGCTTCACAAATTGATAACTATTTAGATCAAAGAGCTGTTGTTATGCAAAATTTAGCTTCATTATTATCTAAATCAATTGAACTAGATAAAGATGTTATGAAAACAGTTGCAGCTTATAGATCTGGAATTAA  >  CP016816/369620‑369869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`